p-bluescript ii ks1 (Agilent technologies)
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p-bluescript ii ks1
P Bluescript Ii Ks1, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bluescript+ii+ks1/pm18924152-23-6-9
Average 90 stars, based on 1 article reviews
P Bluescript Ii Ks1, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bluescript+ii+ks1/pm18924152-23-6-9
Average 90 stars, based on 1 article reviews
p-bluescript ii ks1 - by Bioz Stars,
2026-11
90/100 stars
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Amplification:Article Title: The GacS sensor kinase controls phenotypic reversion of small colony variants isolated from biofilms of Pseudomonas aeruginosa PA14. Article Snippet: A 2-kb fragment of the gacS gene was amplified by PCR (94 1C for 5 min, then 35 cycles consisting of 94 1C for 30 s, 65 1C for 30 s, 72 1C for 2 min, followed by a terminal incubation at 72 1C for 7 min, then held at 15 1C) from P. aeruginosa PA14 genomic DNA using Platinum Pfx polymerase (Invitrogen, Carlsbad, CA) and Prod7 forward and reverse primers. .. Once amplified, the fragment was isolated and ligated into the EcoRV site of Isolation:Article Title: The GacS sensor kinase controls phenotypic reversion of small colony variants isolated from biofilms of Pseudomonas aeruginosa PA14. Article Snippet: A 2-kb fragment of the gacS gene was amplified by PCR (94 1C for 5 min, then 35 cycles consisting of 94 1C for 30 s, 65 1C for 30 s, 72 1C for 2 min, followed by a terminal incubation at 72 1C for 7 min, then held at 15 1C) from P. aeruginosa PA14 genomic DNA using Platinum Pfx polymerase (Invitrogen, Carlsbad, CA) and Prod7 forward and reverse primers. .. Once amplified, the fragment was isolated and ligated into the EcoRV site of Plasmid Preparation:Article Title: The GacS sensor kinase controls phenotypic reversion of small colony variants isolated from biofilms of Pseudomonas aeruginosa PA14. Article Snippet: A 2-kb fragment of the gacS gene was amplified by PCR (94 1C for 5 min, then 35 cycles consisting of 94 1C for 30 s, 65 1C for 30 s, 72 1C for 2 min, followed by a terminal incubation at 72 1C for 7 min, then held at 15 1C) from P. aeruginosa PA14 genomic DNA using Platinum Pfx polymerase (Invitrogen, Carlsbad, CA) and Prod7 forward and reverse primers. .. Once amplified, the fragment was isolated and ligated into the EcoRV site of Construct:Article Title: The GacS sensor kinase controls phenotypic reversion of small colony variants isolated from biofilms of Pseudomonas aeruginosa PA14. Article Snippet: A 2-kb fragment of the gacS gene was amplified by PCR (94 1C for 5 min, then 35 cycles consisting of 94 1C for 30 s, 65 1C for 30 s, 72 1C for 2 min, followed by a terminal incubation at 72 1C for 7 min, then held at 15 1C) from P. aeruginosa PA14 genomic DNA using Platinum Pfx polymerase (Invitrogen, Carlsbad, CA) and Prod7 forward and reverse primers. .. Once amplified, the fragment was isolated and ligated into the EcoRV site of other:Article Title: Role of high-molecular weight tropomyosins in TGF-beta-mediated control of cell motility. Article Snippet: Rat tropomyosin 3 (TM3) cDNA43 was subcloned in Article Title: NG2 Proteoglycan Expression in Mouse Skin: Altered Postnatal Skin Development in the NG2 Null Mouse Article Snippet: In Situ Hybridization A 1-kb fragment corresponding to mouse NG2 base pairs 5458–6358 (AN2) (Schneider et al. 2001) was amplified from mouse RNA by RT-PCR and inserted into the pBluescript II KS(1) vector (Stratagene; La Jolla, CA). Article Title: Zebrafish gonadotropins and their receptors: II. Cloning and characterization of zebrafish follicle-stimulating hormone and luteinizing hormone subunits--their spatial-temporal expression patterns and receptor specificity. Article Snippet: The amplified PCR fragments were resolved on agarose gel, isolated, cloned into Article Title: Characterization ofCampylobacter concisushemolysins Article Snippet: In DNA manipulation techniques, Article Title: NG2-expressing cells in the nervous system revealed by the NG2-EYFP-knockin mouse. Article Snippet: Khalad Karram, Sandra Goebbels, Markus Schwab, Katja Jennissen, Gerald Seifert, Christian Steinhäuser, Klaus-Armin Nave, and Jacqueline Trotter* Molecular Cell Biology, Department of Biology, Johannes Gutenberg University of Mainz, 55128 Mainz, Germany Max Planck Institute of Experimental Medicine, Department of Neurogenetics, 37075 Göttingen, Germany Institute of Cellular Neurosciences, Medical Faculty, University of Bonn, 53105 Bonn, Germany Purification:Article Title: Molecular markers detecting an ectomycorrhizal Suillus collinitus strain on Pinus halepensis roots suggest successful inoculation and persistence in Mediterranean nursery and plantation. Article Snippet: .. It was purified, treated with T4 DNA polymerase and T4 polynucleotide kinase (New England BioLabs Inc., Beverly, MA, USA) (Mello et al., 1999), and cloned into EcoRV-digested and dephosphorylated Clone Assay:Article Title: Molecular markers detecting an ectomycorrhizal Suillus collinitus strain on Pinus halepensis roots suggest successful inoculation and persistence in Mediterranean nursery and plantation. Article Snippet: .. It was purified, treated with T4 DNA polymerase and T4 polynucleotide kinase (New England BioLabs Inc., Beverly, MA, USA) (Mello et al., 1999), and cloned into EcoRV-digested and dephosphorylated |