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bluescript-ks ii plasmid vector  (Agilent technologies)


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    Structured Review

    Agilent technologies bluescript-ks ii plasmid vector
    Bluescript Ks Ii Plasmid Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bluescript+ii+ks++plasmid/pmc01664936-85-13-17
    Average 90 stars, based on 1 article reviews
    bluescript-ks ii plasmid vector - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Amplification:

    Article Title: Human T-cell lymphotrophic virus type-I tax gene induces secretion of human macrophage inflammatory protein-1alpha.
    Article Snippet: E t Human T-cell lymphotropic virus I (HTLV-I) encodes or a 40-kDa protein, Tax, which is important for the mmortalization of T cells.. Tax has been shown to ransactivate several cellular genes.. In this study, we how that MIP-1a is selectively expressed and sereted in the tax transfected Jurkat cell line upon itogen stimulation.

    Article Title: Skipping of exon 14 and possible instability of both the mRNA and the resultant truncated protein underlie a common cholesteryl ester transfer protein deficiency in Japan.
    Article Snippet: .. DNA sequencing was performed in principle by direct sequencing of DNA fragments amplified by asymmetrical PCR.18 Several fragments were also sequenced after subcloning into the plasmid vector Bluescript II (Stratagene). ..

    Plasmid Preparation:

    Article Title: Human T-cell lymphotrophic virus type-I tax gene induces secretion of human macrophage inflammatory protein-1alpha.
    Article Snippet: E t Human T-cell lymphotropic virus I (HTLV-I) encodes or a 40-kDa protein, Tax, which is important for the mmortalization of T cells.. Tax has been shown to ransactivate several cellular genes.. In this study, we how that MIP-1a is selectively expressed and sereted in the tax transfected Jurkat cell line upon itogen stimulation.

    Article Title: A novel endothelial-derived lipase that modulates HDL metabolism.
    Article Snippet: 1Department of Medicine, University of Pennsylvania Health System, Philadelphia, Pennsylvania 19104-6100, USA.. 2Cardiovascular Biology Department, Rhone-Poulenc Rorer Research and Development, Collegeville, Pennsylvania 19426-0107, USA.. Correspondence should be addressed to D.J.R. (e-mail: rader@mail.med.upenn.edu).

    Article Title: Skipping of exon 14 and possible instability of both the mRNA and the resultant truncated protein underlie a common cholesteryl ester transfer protein deficiency in Japan.
    Article Snippet: .. DNA sequencing was performed in principle by direct sequencing of DNA fragments amplified by asymmetrical PCR.18 Several fragments were also sequenced after subcloning into the plasmid vector Bluescript II (Stratagene). ..

    Article Title: Mouse Model of Hyperkinesis Implicates SNAP-25 in Behavioral Regulation
    Article Snippet: .. The nearly full-length SNAP-25 cDNA clone p8.71 ( Oyler et al., 1989 ), which contains the majority of the 5′-untranslated region, the entire open reading encoding the major b isoform expressed in adult brain, and most of the 3′-untranslated region cloned in the plasmid vector Bluescript II KS (Stratagene, San Diego, CA), was modified by PCR to generate a Bsp EI site in the 5′-untranslated region corresponding to the unique but polymorphic Bsp EI site of the genomic DNA clone pG1.1. pG1.1 is a plasmid subclone isolated from a lambda recombinant library constructed of partial Mbo I genomic DNA fragments of strain 129/By mice. ..

    DNA Sequencing:

    Article Title: Skipping of exon 14 and possible instability of both the mRNA and the resultant truncated protein underlie a common cholesteryl ester transfer protein deficiency in Japan.
    Article Snippet: .. DNA sequencing was performed in principle by direct sequencing of DNA fragments amplified by asymmetrical PCR.18 Several fragments were also sequenced after subcloning into the plasmid vector Bluescript II (Stratagene). ..

    Sequencing:

    Article Title: Skipping of exon 14 and possible instability of both the mRNA and the resultant truncated protein underlie a common cholesteryl ester transfer protein deficiency in Japan.
    Article Snippet: .. DNA sequencing was performed in principle by direct sequencing of DNA fragments amplified by asymmetrical PCR.18 Several fragments were also sequenced after subcloning into the plasmid vector Bluescript II (Stratagene). ..

    Polymerase Chain Reaction:

    Article Title: Skipping of exon 14 and possible instability of both the mRNA and the resultant truncated protein underlie a common cholesteryl ester transfer protein deficiency in Japan.
    Article Snippet: .. DNA sequencing was performed in principle by direct sequencing of DNA fragments amplified by asymmetrical PCR.18 Several fragments were also sequenced after subcloning into the plasmid vector Bluescript II (Stratagene). ..

    Article Title: Mouse Model of Hyperkinesis Implicates SNAP-25 in Behavioral Regulation
    Article Snippet: .. The nearly full-length SNAP-25 cDNA clone p8.71 ( Oyler et al., 1989 ), which contains the majority of the 5′-untranslated region, the entire open reading encoding the major b isoform expressed in adult brain, and most of the 3′-untranslated region cloned in the plasmid vector Bluescript II KS (Stratagene, San Diego, CA), was modified by PCR to generate a Bsp EI site in the 5′-untranslated region corresponding to the unique but polymorphic Bsp EI site of the genomic DNA clone pG1.1. pG1.1 is a plasmid subclone isolated from a lambda recombinant library constructed of partial Mbo I genomic DNA fragments of strain 129/By mice. ..

    Subcloning:

    Article Title: Skipping of exon 14 and possible instability of both the mRNA and the resultant truncated protein underlie a common cholesteryl ester transfer protein deficiency in Japan.
    Article Snippet: .. DNA sequencing was performed in principle by direct sequencing of DNA fragments amplified by asymmetrical PCR.18 Several fragments were also sequenced after subcloning into the plasmid vector Bluescript II (Stratagene). ..

    Clone Assay:

    Article Title: Mouse Model of Hyperkinesis Implicates SNAP-25 in Behavioral Regulation
    Article Snippet: .. The nearly full-length SNAP-25 cDNA clone p8.71 ( Oyler et al., 1989 ), which contains the majority of the 5′-untranslated region, the entire open reading encoding the major b isoform expressed in adult brain, and most of the 3′-untranslated region cloned in the plasmid vector Bluescript II KS (Stratagene, San Diego, CA), was modified by PCR to generate a Bsp EI site in the 5′-untranslated region corresponding to the unique but polymorphic Bsp EI site of the genomic DNA clone pG1.1. pG1.1 is a plasmid subclone isolated from a lambda recombinant library constructed of partial Mbo I genomic DNA fragments of strain 129/By mice. ..

    Modification:

    Article Title: Mouse Model of Hyperkinesis Implicates SNAP-25 in Behavioral Regulation
    Article Snippet: .. The nearly full-length SNAP-25 cDNA clone p8.71 ( Oyler et al., 1989 ), which contains the majority of the 5′-untranslated region, the entire open reading encoding the major b isoform expressed in adult brain, and most of the 3′-untranslated region cloned in the plasmid vector Bluescript II KS (Stratagene, San Diego, CA), was modified by PCR to generate a Bsp EI site in the 5′-untranslated region corresponding to the unique but polymorphic Bsp EI site of the genomic DNA clone pG1.1. pG1.1 is a plasmid subclone isolated from a lambda recombinant library constructed of partial Mbo I genomic DNA fragments of strain 129/By mice. ..

    Isolation:

    Article Title: Mouse Model of Hyperkinesis Implicates SNAP-25 in Behavioral Regulation
    Article Snippet: .. The nearly full-length SNAP-25 cDNA clone p8.71 ( Oyler et al., 1989 ), which contains the majority of the 5′-untranslated region, the entire open reading encoding the major b isoform expressed in adult brain, and most of the 3′-untranslated region cloned in the plasmid vector Bluescript II KS (Stratagene, San Diego, CA), was modified by PCR to generate a Bsp EI site in the 5′-untranslated region corresponding to the unique but polymorphic Bsp EI site of the genomic DNA clone pG1.1. pG1.1 is a plasmid subclone isolated from a lambda recombinant library constructed of partial Mbo I genomic DNA fragments of strain 129/By mice. ..

    Recombinant:

    Article Title: Mouse Model of Hyperkinesis Implicates SNAP-25 in Behavioral Regulation
    Article Snippet: .. The nearly full-length SNAP-25 cDNA clone p8.71 ( Oyler et al., 1989 ), which contains the majority of the 5′-untranslated region, the entire open reading encoding the major b isoform expressed in adult brain, and most of the 3′-untranslated region cloned in the plasmid vector Bluescript II KS (Stratagene, San Diego, CA), was modified by PCR to generate a Bsp EI site in the 5′-untranslated region corresponding to the unique but polymorphic Bsp EI site of the genomic DNA clone pG1.1. pG1.1 is a plasmid subclone isolated from a lambda recombinant library constructed of partial Mbo I genomic DNA fragments of strain 129/By mice. ..

    Construct:

    Article Title: Mouse Model of Hyperkinesis Implicates SNAP-25 in Behavioral Regulation
    Article Snippet: .. The nearly full-length SNAP-25 cDNA clone p8.71 ( Oyler et al., 1989 ), which contains the majority of the 5′-untranslated region, the entire open reading encoding the major b isoform expressed in adult brain, and most of the 3′-untranslated region cloned in the plasmid vector Bluescript II KS (Stratagene, San Diego, CA), was modified by PCR to generate a Bsp EI site in the 5′-untranslated region corresponding to the unique but polymorphic Bsp EI site of the genomic DNA clone pG1.1. pG1.1 is a plasmid subclone isolated from a lambda recombinant library constructed of partial Mbo I genomic DNA fragments of strain 129/By mice. ..

    other:

    Article Title: Rat transcobalamin: cloning and regulation of mRNA expression
    Article Snippet: The two clones were then subcloned into the Eco R 1 site of bluescript-KS II plasmid vector (Stratagene).

    Article Title: Neovascularization in the anterior segment of the rabbit eye by experimental anterior ischemia.
    Article Snippet: Received: 1 March 2001 Revised: 12 November 2001 Accepted: 26 November 2001 Published online: 29 January 2002 © Springer-Verlag 2002 Abstract Purpose: To investigate the effects of anterior ischemia accompanied by neither retinal nor choroidal ischemia on the anterior segment of the eye.. Methods: Both long posterior ciliary arteries in the right eye of 14 rabbits were directly cauterized with an electric coagulator.. The eyes were enucleated 1, 2, 4, 7, 9 or 14 days after cauterization, then fixed with 4% paraformaldehyde.



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