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Jackson Immuno blocking buffer
Blocking Buffer, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 3759 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blocking+buffer/Normal+Goat+Serum/pm42904888-85-9-18
Average 96 stars, based on 3759 article reviews

blocking buffer - by Bioz Stars, 2026-11

96/100 stars

Images

Recombinant:

Article Title: Neural coordination of wing and leg movements during a terrestrial threat display.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-DsRed (rabbit) Takara Bio Cat#632496; RRID: AB_10013483 anti-Bruchpilot (mouse) Developmental Studies Hybridoma Bank Cat#nc82; RRID: AB_2314866 anti-GFP (rabbit) Millipore Cat# AB3080P; RRID: AB_2630379 Goat anti-rabbit IgG (H+L) Alexa Fluor 488 Thermo Fisher Scientific Cat#A11008; RRID: AB_143165 Goat anti-rabbit IgG (H+L) Alexa Fluor 568 Thermo Fisher Scientific Cat# A-11011; RRID: AB_143157 Goat anti-mouse IgG (H+L) Alexa Fluor 633 Thermo Fisher Scientific Cat#A21050; RRID: AB_141431 Biological samples Normal Goat Serum Jackson ImmunoResearch Labs Cat005-000-121; RRID: AB_2336990 Tropicana apple juice Amazon Cat#B007SNJDXM Chemicals, peptides, and recombinant proteins Paraformaldehyde, 16% solution, EM grade Electron Microscopy Sciences Cat#15710 (30525-89-4) all trans-Retinal Millipore Sigma Cat#R2500 (116-31-4) Hydrocarbon Soluble Siliconizing Fluid Thermo Fisher Scientific Cat#TS-42800 (474-02-4) Fluon (PTFE-30) Tar Heel Ants 9002-84-0 Critical commercial assays Gateway LR Clonase II Enzyme mix Thermo Fisher Scientific Cat#11791020 Deposited data Data This study https://doi.org/10.22002/ 0086c-qa365 Experimental models: Organisms/strains SplitThr Anderson Lab N/A 20E08-LexA (VK22) Anderson Lab N/A SS33004 (DNp60-split2) Jens Goldammer N/A VT058429EB1-AD (attP40) This study N/A VT058429EB2-AD (attP40) This study N/A VT058429EB3-AD (attP40) This study N/A VT058429EB4-AD (attP40) This study N/A VT058429EB5-AD (attP40) This study N/A 12G09EB1-DBD (attP2) This study N/A 12G09EB2-DBD (attP2) This study N/A 12G09EB3-DBD (attP2) This study N/A 12G09EB4-DBD (attP2) This study N/A 12G09EB5-DBD (attP2) This study N/A w[1118]; P{y[+t7.7] w[+mC]=VT058429-p65.AD}attP40 BDSC BDSC_74085 w[1118]; P{y[+t7.7] w[+mC]=R12G09-GAL4.DBD}attP2 BDSC BDSC_69079 w[1118]; P{y[+t7.7] w[+mC]=VT061518-GAL4.DBD}attP2 BDSC BDSC_72775 w[1118]; P{y[+t7.7] w[+mC]=VT045669-GAL4.DBD}attP2 BDSC BDSC_72226 w[1118]; P{y[+t7.7] w[+mC]=R54A01-p65.AD}attP40 BDSC BDSC_71091 w[1118]; P{y[+t7.7] w[+mC]=VT056582-GAL4.DBD}attP2 BDSC BDSC_75127 (Continued on next page) ll OPEN ACCESS Current Biology 36, 1–18.e1–e5, November 2, 2026 e1 Article ..

Article Title: Molecular mechanism of residue-specific nucleosome acetylation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-Histone H3 Abcam Cat# ab10799; RRID: AB_470239 Rabbit anti-Histone H4K16ac Millipore Cat# 07-329; RRID: AB_310525 Rabbit anti-Histone H4K12ac Millipore Cat# 07-595; RRID: AB_310740 Rabbit anti-Histone H4K5ac Active Motif Cat# 39699; RRID: AB_2793311 Mouse anti-GFP Roche Cat# 11814460001; RRID: AB_390913 Rabbit anti-MSL1 Gilfillan et al.104 N/A Guinea pig anti-MSL2 Albig et al.105 N/A Rabbit anti-MSL2 Straub et al.106 N/A Rabbit anti-MOF Prestel et al.107 N/A Rat anti-MSL3 clone 1C9 Straub et al.108 N/A Mouse anti-Lamin (T40) Saumweber/Becker laboratories N/A Goat anti-rabbit-IRDye680RD LICORbio Cat# 926-68071; RRID: AB_10956166 Goat anti-rabbit-IRDye800CW LICORbio Cat# 926-32211; RRID: AB_621843 Goat anti-mouse-IRDye680RD LICORbio Cat# 926-68070; RRID: AB_10956588 Goat anti-mouse-IRDye800CW LICORbio Cat# 926-32210; RRID: AB_621842 Donkey anti-guinea-pig-IRDye800CW LICORbio Cat# 926-32411; RRID: AB_1850024 Donkey anti-mouse-Alexa488 Jackson Immuno Research Cat# 715-545-151; RRID: AB_2341099 Donkey anti-rat-Cy3 Jackson Immuno Research Cat# 712-165-153; RRID: AB_2340667 Donkey anti-rabbit-Alexa647 Jackson Immuno Research Cat# 711-605-152; RRID: AB_2492288 Bacterial and virus strains E. coli chemically competent DH5α-T1R ThermoFisher Cat# 12297016 E. coli electrocompetent DH10MultiBac Geneva Biotech N/A E. coli chemically competent BL21 Star (DE3) pLysS ThermoFisher Cat# C602003 Chemicals, peptides, and recombinant proteins Sf-900 II SFM ThermoFisher Cat# 10902088 Gibco Schneider’s Drosophila medium ThermoFisher Cat# 21720001 FBS supreme, fetal bovine serum PAN-Biotech Cat# P30-3031 Effectene Transfection Reagent Qiagen Cat# 301425 Blasticidin S Gibco Cat# A11139-03 Formaldehyde, methanol-free Polysciences Cat# 04018-1 Normal Goat Serum Jackson Immuno Research Cat# 005-000-121 DAPI ThermoFisher Cat# D1306 VECTASHIELD Antifade Mounting Medium Vector Laboratories Cat# H-1000-10 Human histones H2A, H2B, H3, H4 The Histone Source, Colorado State University Cat# HH2A; HH2B; HH3; HH4 Human histone H3K36me3 and matching human histone H3 Biosyntan N/A Latency-associated nuclear antigen peptide (MAPPGMRLRSGRSTGAPLTRGS) Peptide Specialty Laboratories N/A Latency-associated nuclear antigen peptide, R9E mutant (MAPPGMRLESGRSTGAPLTRGS) Peptide Specialty Laboratories N/A Anti-DYKDDDDK G1 Affinity Resin Genscript Corporation Cat# L00432-1 (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 86, 1–18.e1–e10, November 19, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER FLAG peptide MedChemExpress Cat# HY-P0223 Acetyl Coenzyme A trisodium salt Sigma-Aldrich Cat# A2056 Deposited data MSL HAT-NCP This study PDB: 9R2U EMDB ID: 53542 (composite map) 53541 (overall refinement) 53540 (local refinement) MSL HAT- H3K36me3 NCP This study PDB: 9R2Y EMDB ID: 53546 (composite map) 53545 (overall refinement) 53544 (local refinement) MSL3 CD – H3K36me3 NCP This study PDB: 9R2W EMDB ID: 53543 Cryo-EM structure of nucleosome core particle composed of the Widom 601 DNA sequence Wang et al.109 PDB: 7OHC Imaging data, Fiji macro and R codes This study Zenodo:https://doi.org/10.5281/zenodo.

Electron Microscopy:

Virus:

Drosophila:

Transfection:

Mutagenesis:

Laser-Scanning Microscopy:

Article Title: Lymphangiogenesis driven by VEGF-C reshapes tumor immune landscape and enables tumor eradication by viral immunotherapy in mouse melanoma models.
Article Snippet: Vero african green monkey kidney epithelial cells (ATCC Cat# .. REAGENT or RESOURCE SOURCE IDENTIFIER ZEISS LSM 780 Laser Scanning Microscope Zeiss RRID: SCR_020922 Leica Bond RX Automated Stainer Leica Biosystems Cat# 21.2821 Octomacs Dissociator Miltenyi Cat# 130–134-029 Countess II Automated Cell Counter Thermo Fisher Scientific RRID: SCR_025370 Nanazoomer Digital Slide Scanner Hamamatsu 40 μm filter Fisher Scientific Cat# 22–363-547 70 μm filter Fisher Scientific Cat# 22–363-548 Acrylamide Biorad Cat# 161–0148 Bovine Serum Albumin (Fraction V) Fisher Cat# BP1600-100 Buffer RLT Plus Qiagen Cat# 1053393 Collagenase D Roche Cat# 50–100-3282 Cytoseal XYL Fisher Scientific Cat# 50–209-0573 Dako Dual Endogenous Enzyme Block Agilent Cat# S2003 DAPI Sigma-Aldrich Cat# D9542 DMEM – high glucose, no L-glutamine Gibco Cat# 11960–044 Fetal Bovine Serum (FBS) Corning Cat# 35–010-CV Fluorescence Mounting Medium Dako/Agilent Cat# S3023 Glutamax 100X Invitrogen Cat# 35050061 Glycergel Aqueous Mounting Media DAKO Cat# C0563 Goat Serum Jackson Immunoresearch Cat# 005–000-121 IHC/ISH Superblock Solution Leica Biosystems Cat# PV6122 Laemmli buffer Bioworld Cat# 10570021 L-glutamine Sigma Cat# D6429 NaN3 Fisher Scientific Cat# S0489100G OCT Compound Thermo Fisher Scientific Cat# 23–730-571 PBS Gibco Cat# 14–190-250 Penicillin-Streptomycin Gibco Cat# 15–070-063 PowerUpTM SYBRTM Green Master Mix Applied Biosystems Cat# A25741 Puromycin Sigma-Aldrich Cat# 540411 PVDF membrane Biorad Cat# 1620177 RBC lysis buffer eBioscience Cat# 00–4300-54 Triton X-100 Sigma-Aldrich Cat# 93443 Tween 20 Sigma-Aldrich Cat# P1379 Fixable Viability Dye eFluorTM 780 Thermo Fisher Scientific Cat #. .. 65–0865-14 22 Cell Reports 45, 118130, October 27, 2026 CCL-81) were maintained in DMEM medium supplemented with 10% FBS, 1% Glutamax-100X (Invitrogen), penicillin and streptomycin.1 All cell lines were IMPACT tested for mycoplasma and other mouse pathogens.

Automated:

Blocking Assay:

Article Title: Antisense Oligonucleotides that Are Toxic to Prion-Infected Mice Share an Emergent Mechanism.
Article Snippet: Some, but not all, gapmer antisense oligonucleotides (ASOs) delivered into cerebrospinal fluid exhibit fatal subacute toxicity in late-stage prion-infected mice but not in uninfected mice.. Here, we performed a battery of in vivo studies demonstrating that the toxicity is compoundand diseasestage-dependent, and not predicted by behavioral or molecular measurements in uninfected animals.. A panel of toxic ASOs all elicited a consistent transcriptomic response signature in prion-infected mice, which emerged over the course of 7 days post dose.

Article Title: USP10 Silencing Prevents Scarring and Inflammation in a Modified Rabbit Model of Glaucoma Filtration Surgery.
Article Snippet: .. To reduce nonspecific binding, the sections were treated with blocking buffer containing 10% normal goat serum in PBS (Jackson ImmunoResearch Labs) for 20 minutes. ..

Article Title: Sex chromosome and gonadal regulation of cardiac myofibroblast activation decoupled using mechanically tunable hydrogels and the Four Core Genotypes model
Article Snippet: Next, slides were rinsed three times with PBS then permeabilized with 0.1% Triton-X/PBS (Fisher Scientific, Cat. No. AC215682500) for 30 minutes. .. Slides were rinsed three times and placed in blocking buffer solution containing 4% Normal Goat Serum (NGS, Jackson ImmunoResearch, Cat. No. 005-000-121) and 5% bovine serum albumin (BSA, Millipore Sigma, Cat. No. A9576) for 1 hour at RT. ..

Fluorescence:

Immunohistochemistry:

Membrane:

Red Blood Cell Lysis:

Next-Generation Sequencing:

Article Title: Electrical synapses mediate visual orientation behavior.
Article Snippet: After the injection, neurobiotin was allowed to diffuse via gap junctions for ∼30 min. Immunohistochemistry Following the fillings, the brains were fixed with 4% v/v PFA (158127, Sigma-Aldrich) in PBS for 30 min at room temperature. .. Next, the fixative was washed out with PBS, the brains solubilized in PBST (0.5% Triton X100, T9284, Sigma-Aldrich) for 1 h, and blocked in 7.5% v/v NGS (Normal Goat Serum, 005-000-121, Jackson ImmunoResearch) in PBST for 1-2 h at room temperature. .. Brains were then incubated in primary antibodies: chicken anti-GFP (1:1000, Abcam #ab13970), mouse anti-Bruchpilot (1:10, DSHB nc82, RRID: AB_2314866), and streptavidin-AF647 (1:500, Invitrogen S21374) for 4 h at room temperature and 24 h at 4◦C.

Allele-specific Oligonucleotide:

Incubation:

Labeling:

Nonspecific Binding:

Organoids:

Article Title: Workflow and instrumentation for multiscale correlative imaging in voluminous cryogenic specimens
Article Snippet: The IF buffer contained 7.6 mg/ml NaCl (Sigma-Aldrich, Cat.#1.06404), Tween-20 (Sigma-Aldrich, Cat.#P9416), 1.87 mg/ml Na 2 HPO 4 *7H 2 O (Sigma-Aldrich, Cat.#S9390), 0.4 mg/ml NaH 2 PO 4 *H 2 O (Sigma-Aldrich, Cat.#S9638), 0.5 mg/ml NaN 3 (Sigma-Aldrich, Cat.#S8032), 10 mg/ml BSA (Sigma-Aldrich, Cat.#A9418), 20 μl/ml Triton X-100 (Sigma-Aldrich, Cat.#T8787) at final concentrations. .. Permeabilized organoids were blocked with 10% goat serum (Jackson Immunoresearch, Cat.# 005-000-121) for 4 hours at 4 °C. .. Primary antibodies (Mouse-anti GM130 1:100 (BDbiosciences, Cat.# 610823), Rat anti-E-Cadherin 1:150 (Thermo Fisher Scientific, Cat.# 13-1900), Rabbit polyclonal anti-ZO1 1:500 (Thermo Fischer Scientific, Cat.# 61-7300)) were diluted in the primary blocking buffer (5% goat serum in IF buffer) and incubated overnight at 4°C.

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