Activity Assay:Article Title: RECQL4 requires PARP1 for recruitment to DNA damage, and PARG dePARylation facilitates its associated role in end joining.
Article Snippet: The cells were collected 48 h after transfection.The cells were collected 48 h after transfection.. The antibodies used were RECQL4 (made in house)11,40, BLM (NB100-214 Novus Biologicals), GFP (sc-8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell Signaling), PAR (4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960-CUS, Invitrogen) and Tubulin (sc5286, Santa Cruz).
Article Title: PARP1 regulates RECQL4 recruitment for DNA double-strand break repair
Article Snippet: Cells were harvested 48 h after transfection.Cells were harvested 48 h after transfection.. Antibodies used were RECQL4 (in house) 11,36, BLM (NB100-214 Novus Biologicals, GFP (sc8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell signaling), PAR ( 4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960CUS, Invitrogen) and Tubulin (sc-5286, Santa Cruz).. As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.
Control:Article Title: RECQL4 requires PARP1 for recruitment to DNA damage, and PARG dePARylation facilitates its associated role in end joining.
Article Snippet: The cells were collected 48 h after transfection.The cells were collected 48 h after transfection.. The antibodies used were RECQL4 (made in house)11,40, BLM (NB100-214 Novus Biologicals), GFP (sc-8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell Signaling), PAR (4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960-CUS, Invitrogen) and Tubulin (sc5286, Santa Cruz).
Article Title: PARP1 regulates RECQL4 recruitment for DNA double-strand break repair
Article Snippet: Cells were harvested 48 h after transfection.Cells were harvested 48 h after transfection.. Antibodies used were RECQL4 (in house) 11,36, BLM (NB100-214 Novus Biologicals, GFP (sc8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell signaling), PAR ( 4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960CUS, Invitrogen) and Tubulin (sc-5286, Santa Cruz).. As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.
Inhibition:Article Title: RECQL4 requires PARP1 for recruitment to DNA damage, and PARG dePARylation facilitates its associated role in end joining.
Article Snippet: The cells were collected 48 h after transfection.The cells were collected 48 h after transfection.. The antibodies used were RECQL4 (made in house)11,40, BLM (NB100-214 Novus Biologicals), GFP (sc-8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell Signaling), PAR (4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960-CUS, Invitrogen) and Tubulin (sc5286, Santa Cruz).
Article Title: PARP1 regulates RECQL4 recruitment for DNA double-strand break repair
Article Snippet: Cells were harvested 48 h after transfection.Cells were harvested 48 h after transfection.. Antibodies used were RECQL4 (in house) 11,36, BLM (NB100-214 Novus Biologicals, GFP (sc8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell signaling), PAR ( 4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960CUS, Invitrogen) and Tubulin (sc-5286, Santa Cruz).. As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.
Transfection:Article Title: RECQL4 requires PARP1 for recruitment to DNA damage, and PARG dePARylation facilitates its associated role in end joining.
Article Snippet: The cells were collected 48 h after transfection.The cells were collected 48 h after transfection.. The antibodies used were RECQL4 (made in house)11,40, BLM (NB100-214 Novus Biologicals), GFP (sc-8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell Signaling), PAR (4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960-CUS, Invitrogen) and Tubulin (sc5286, Santa Cruz).
Article Title: PARP1 regulates RECQL4 recruitment for DNA double-strand break repair
Article Snippet: Cells were harvested 48 h after transfection.Cells were harvested 48 h after transfection.. Antibodies used were RECQL4 (in house) 11,36, BLM (NB100-214 Novus Biologicals, GFP (sc8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell signaling), PAR ( 4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960CUS, Invitrogen) and Tubulin (sc-5286, Santa Cruz).. As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.
Software:Article Title: RECQL4 requires PARP1 for recruitment to DNA damage, and PARG dePARylation facilitates its associated role in end joining.
Article Snippet: The cells were collected 48 h after transfection.The cells were collected 48 h after transfection.. The antibodies used were RECQL4 (made in house)11,40, BLM (NB100-214 Novus Biologicals), GFP (sc-8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell Signaling), PAR (4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960-CUS, Invitrogen) and Tubulin (sc5286, Santa Cruz).
Article Title: PARP1 regulates RECQL4 recruitment for DNA double-strand break repair
Article Snippet: Cells were harvested 48 h after transfection.Cells were harvested 48 h after transfection.. Antibodies used were RECQL4 (in house) 11,36, BLM (NB100-214 Novus Biologicals, GFP (sc8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell signaling), PAR ( 4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960CUS, Invitrogen) and Tubulin (sc-5286, Santa Cruz).. As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.
Expressing:Article Title: RECQL4 requires PARP1 for recruitment to DNA damage, and PARG dePARylation facilitates its associated role in end joining.
Article Snippet: The cells were collected 48 h after transfection.The cells were collected 48 h after transfection.. The antibodies used were RECQL4 (made in house)11,40, BLM (NB100-214 Novus Biologicals), GFP (sc-8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell Signaling), PAR (4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960-CUS, Invitrogen) and Tubulin (sc5286, Santa Cruz).
Article Title: PARP1 regulates RECQL4 recruitment for DNA double-strand break repair
Article Snippet: Cells were harvested 48 h after transfection.Cells were harvested 48 h after transfection.. Antibodies used were RECQL4 (in house) 11,36, BLM (NB100-214 Novus Biologicals, GFP (sc8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell signaling), PAR ( 4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960CUS, Invitrogen) and Tubulin (sc-5286, Santa Cruz).. As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.
Staining:Article Title: RECQL4 requires PARP1 for recruitment to DNA damage, and PARG dePARylation facilitates its associated role in end joining.
Article Snippet: The cells were collected 48 h after transfection.The cells were collected 48 h after transfection.. The antibodies used were RECQL4 (made in house)11,40, BLM (NB100-214 Novus Biologicals), GFP (sc-8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell Signaling), PAR (4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960-CUS, Invitrogen) and Tubulin (sc5286, Santa Cruz).
Article Title: PARP1 regulates RECQL4 recruitment for DNA double-strand break repair
Article Snippet: Cells were harvested 48 h after transfection.Cells were harvested 48 h after transfection.. Antibodies used were RECQL4 (in house) 11,36, BLM (NB100-214 Novus Biologicals, GFP (sc8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell signaling), PAR ( 4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960CUS, Invitrogen) and Tubulin (sc-5286, Santa Cruz).. As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.
In Vitro:Article Title: RECQL4 requires PARP1 for recruitment to DNA damage, and PARG dePARylation facilitates its associated role in end joining.
Article Snippet: The cells were collected 48 h after transfection.The cells were collected 48 h after transfection.. The antibodies used were RECQL4 (made in house)11,40, BLM (NB100-214 Novus Biologicals), GFP (sc-8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell Signaling), PAR (4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960-CUS, Invitrogen) and Tubulin (sc5286, Santa Cruz).
Article Title: PARP1 regulates RECQL4 recruitment for DNA double-strand break repair
Article Snippet: Cells were harvested 48 h after transfection.Cells were harvested 48 h after transfection.. Antibodies used were RECQL4 (in house) 11,36, BLM (NB100-214 Novus Biologicals, GFP (sc8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell signaling), PAR ( 4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960CUS, Invitrogen) and Tubulin (sc-5286, Santa Cruz).. As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.
Derivative Assay:Article Title: RECQL4 requires PARP1 for recruitment to DNA damage, and PARG dePARylation facilitates its associated role in end joining.
Article Snippet: The cells were collected 48 h after transfection.The cells were collected 48 h after transfection.. The antibodies used were RECQL4 (made in house)11,40, BLM (NB100-214 Novus Biologicals), GFP (sc-8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell Signaling), PAR (4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960-CUS, Invitrogen) and Tubulin (sc5286, Santa Cruz).
Article Title: PARP1 regulates RECQL4 recruitment for DNA double-strand break repair
Article Snippet: Cells were harvested 48 h after transfection.Cells were harvested 48 h after transfection.. Antibodies used were RECQL4 (in house) 11,36, BLM (NB100-214 Novus Biologicals, GFP (sc8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell signaling), PAR ( 4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960CUS, Invitrogen) and Tubulin (sc-5286, Santa Cruz).. As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.
Western Blot:Article Title: RECQL4 requires PARP1 for recruitment to DNA damage, and PARG dePARylation facilitates its associated role in end joining.
Article Snippet: The cells were collected 48 h after transfection.The cells were collected 48 h after transfection.. The antibodies used were RECQL4 (made in house)11,40, BLM (NB100-214 Novus Biologicals), GFP (sc-8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell Signaling), PAR (4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960-CUS, Invitrogen) and Tubulin (sc5286, Santa Cruz).
Article Title: PARP1 regulates RECQL4 recruitment for DNA double-strand break repair
Article Snippet: Cells were harvested 48 h after transfection.Cells were harvested 48 h after transfection.. Antibodies used were RECQL4 (in house) 11,36, BLM (NB100-214 Novus Biologicals, GFP (sc8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell signaling), PAR ( 4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960CUS, Invitrogen) and Tubulin (sc-5286, Santa Cruz).. As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.
Binding Assay:Article Title: RECQL4 requires PARP1 for recruitment to DNA damage, and PARG dePARylation facilitates its associated role in end joining.
Article Snippet: The cells were collected 48 h after transfection.The cells were collected 48 h after transfection.. The antibodies used were RECQL4 (made in house)11,40, BLM (NB100-214 Novus Biologicals), GFP (sc-8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell Signaling), PAR (4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960-CUS, Invitrogen) and Tubulin (sc5286, Santa Cruz).
Article Title: PARP1 regulates RECQL4 recruitment for DNA double-strand break repair
Article Snippet: Cells were harvested 48 h after transfection.Cells were harvested 48 h after transfection.. Antibodies used were RECQL4 (in house) 11,36, BLM (NB100-214 Novus Biologicals, GFP (sc8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell signaling), PAR ( 4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960CUS, Invitrogen) and Tubulin (sc-5286, Santa Cruz).. As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.
Marker:Article Title: RECQL4 requires PARP1 for recruitment to DNA damage, and PARG dePARylation facilitates its associated role in end joining.
Article Snippet: The cells were collected 48 h after transfection.The cells were collected 48 h after transfection.. The antibodies used were RECQL4 (made in house)11,40, BLM (NB100-214 Novus Biologicals), GFP (sc-8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell Signaling), PAR (4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960-CUS, Invitrogen) and Tubulin (sc5286, Santa Cruz).
Article Title: PARP1 regulates RECQL4 recruitment for DNA double-strand break repair
Article Snippet: Cells were harvested 48 h after transfection.Cells were harvested 48 h after transfection.. Antibodies used were RECQL4 (in house) 11,36, BLM (NB100-214 Novus Biologicals, GFP (sc8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell signaling), PAR ( 4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960CUS, Invitrogen) and Tubulin (sc-5286, Santa Cruz).. As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.
Knockdown:Article Title: RECQL4 requires PARP1 for recruitment to DNA damage, and PARG dePARylation facilitates its associated role in end joining.
Article Snippet: The cells were collected 48 h after transfection.The cells were collected 48 h after transfection.. The antibodies used were RECQL4 (made in house)11,40, BLM (NB100-214 Novus Biologicals), GFP (sc-8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell Signaling), PAR (4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960-CUS, Invitrogen) and Tubulin (sc5286, Santa Cruz).
Article Title: PARP1 regulates RECQL4 recruitment for DNA double-strand break repair
Article Snippet: Cells were harvested 48 h after transfection.Cells were harvested 48 h after transfection.. Antibodies used were RECQL4 (in house) 11,36, BLM (NB100-214 Novus Biologicals, GFP (sc8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell signaling), PAR ( 4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960CUS, Invitrogen) and Tubulin (sc-5286, Santa Cruz).. As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.
Amplification:Article Title: RECQL4 requires PARP1 for recruitment to DNA damage, and PARG dePARylation facilitates its associated role in end joining.
Article Snippet: The cells were collected 48 h after transfection.The cells were collected 48 h after transfection.. The antibodies used were RECQL4 (made in house)11,40, BLM (NB100-214 Novus Biologicals), GFP (sc-8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell Signaling), PAR (4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960-CUS, Invitrogen) and Tubulin (sc5286, Santa Cruz).
Article Title: PARP1 regulates RECQL4 recruitment for DNA double-strand break repair
Article Snippet: Cells were harvested 48 h after transfection.Cells were harvested 48 h after transfection.. Antibodies used were RECQL4 (in house) 11,36, BLM (NB100-214 Novus Biologicals, GFP (sc8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell signaling), PAR ( 4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960CUS, Invitrogen) and Tubulin (sc-5286, Santa Cruz).. As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.
Construct:Article Title: RECQL4 requires PARP1 for recruitment to DNA damage, and PARG dePARylation facilitates its associated role in end joining.
Article Snippet: The cells were collected 48 h after transfection.The cells were collected 48 h after transfection.. The antibodies used were RECQL4 (made in house)11,40, BLM (NB100-214 Novus Biologicals), GFP (sc-8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell Signaling), PAR (4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960-CUS, Invitrogen) and Tubulin (sc5286, Santa Cruz).
Article Title: PARP1 regulates RECQL4 recruitment for DNA double-strand break repair
Article Snippet: Cells were harvested 48 h after transfection.Cells were harvested 48 h after transfection.. Antibodies used were RECQL4 (in house) 11,36, BLM (NB100-214 Novus Biologicals, GFP (sc8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell signaling), PAR ( 4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960CUS, Invitrogen) and Tubulin (sc-5286, Santa Cruz).. As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.
Plasmid Preparation:Article Title: RECQL4 requires PARP1 for recruitment to DNA damage, and PARG dePARylation facilitates its associated role in end joining.
Article Snippet: The cells were collected 48 h after transfection.The cells were collected 48 h after transfection.. The antibodies used were RECQL4 (made in house)11,40, BLM (NB100-214 Novus Biologicals), GFP (sc-8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell Signaling), PAR (4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960-CUS, Invitrogen) and Tubulin (sc5286, Santa Cruz).
Article Title: PARP1 regulates RECQL4 recruitment for DNA double-strand break repair
Article Snippet: Cells were harvested 48 h after transfection.Cells were harvested 48 h after transfection.. Antibodies used were RECQL4 (in house) 11,36, BLM (NB100-214 Novus Biologicals, GFP (sc8334, Santa Cruz), DNA ligase I (sc-20222, Santa Cruz), DNA ligase III (1F3, GeneTex), PARP1 (9542L, Cell signaling), PAR ( 4336-BPC-100, Trevigen), XRCC1 (GTX23133, GeneTex), FLAG (F1804, Sigma), V5 (R960CUS, Invitrogen) and Tubulin (sc-5286, Santa Cruz).. As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.As previously described 18, the recombinant RECQL4 protein was purified from the BL21 E. coli system and BLM was purified using a baculovirus/insect cell expression system.
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