blm Search Results


blm antibody  (Bioss)
93
Bioss blm antibody
Blm Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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gene exp blm hs00172060 m1  (Thermo Fisher)
87
Thermo Fisher gene exp blm hs00172060 m1
Gene Exp Blm Hs00172060 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 87 stars, based on 1 article reviews
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a300 110a  (Bethyl)
95
Bethyl a300 110a
A300 110a, supplied by Bethyl, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
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blm  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology blm
FIGURE 3: Depletion <t>of</t> <t>Smc5</t> or Smc6 disrupts chromosome segregation. (A) Example images of the anaphase bridges (red arrowheads) and lagging chromosomes (green arrowheads) frequently seen in Smc5/6-depleted cells. Scale bar: 10 μm. (B) Examples of the formation of micronuclei in Smc5- and Smc6-depleted cells. Green arrows indicate micronuclei containing the centromere marker CREST; red arrows indicate micronuclei negative for the CREST signal. Scale bar: 10 μm. (C) Representative images of PICH (red) and <t>BLM</t> (green) immunofluorescence in anaphase bridges. DNA was counterstained with DAPI (blue). Scale bar: 10 μm. (D) Frequency of anaphase bridges and lagging chromosomes within the anaphase chromosomes observed in the control and siRNA-treated cells. Three hundred anaphases were analyzed per condition. Bar graph shows mean ± SD from three independent experiments; p value < 0.05; **, p = 0.001–0.01; two-tailed Student’s t test. (E) Frequency of anaphase bridges positive for PICH and BLM, PICH only, or BLM only. One hundred anaphase bridges were analyzed for each sample.
Blm, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/blm/product/Santa Cruz Biotechnology
Average 94 stars, based on 1 article reviews
blm - by Bioz Stars, 2026-03
94/100 stars
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blm  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc blm
FIGURE 3: Depletion <t>of</t> <t>Smc5</t> or Smc6 disrupts chromosome segregation. (A) Example images of the anaphase bridges (red arrowheads) and lagging chromosomes (green arrowheads) frequently seen in Smc5/6-depleted cells. Scale bar: 10 μm. (B) Examples of the formation of micronuclei in Smc5- and Smc6-depleted cells. Green arrows indicate micronuclei containing the centromere marker CREST; red arrows indicate micronuclei negative for the CREST signal. Scale bar: 10 μm. (C) Representative images of PICH (red) and <t>BLM</t> (green) immunofluorescence in anaphase bridges. DNA was counterstained with DAPI (blue). Scale bar: 10 μm. (D) Frequency of anaphase bridges and lagging chromosomes within the anaphase chromosomes observed in the control and siRNA-treated cells. Three hundred anaphases were analyzed per condition. Bar graph shows mean ± SD from three independent experiments; p value < 0.05; **, p = 0.001–0.01; two-tailed Student’s t test. (E) Frequency of anaphase bridges positive for PICH and BLM, PICH only, or BLM only. One hundred anaphase bridges were analyzed for each sample.
Blm, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/blm/product/Cell Signaling Technology Inc
Average 94 stars, based on 1 article reviews
blm - by Bioz Stars, 2026-03
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gfp blm  (Addgene inc)
93
Addgene inc gfp blm
FIGURE 3: Depletion <t>of</t> <t>Smc5</t> or Smc6 disrupts chromosome segregation. (A) Example images of the anaphase bridges (red arrowheads) and lagging chromosomes (green arrowheads) frequently seen in Smc5/6-depleted cells. Scale bar: 10 μm. (B) Examples of the formation of micronuclei in Smc5- and Smc6-depleted cells. Green arrows indicate micronuclei containing the centromere marker CREST; red arrows indicate micronuclei negative for the CREST signal. Scale bar: 10 μm. (C) Representative images of PICH (red) and <t>BLM</t> (green) immunofluorescence in anaphase bridges. DNA was counterstained with DAPI (blue). Scale bar: 10 μm. (D) Frequency of anaphase bridges and lagging chromosomes within the anaphase chromosomes observed in the control and siRNA-treated cells. Three hundred anaphases were analyzed per condition. Bar graph shows mean ± SD from three independent experiments; p value < 0.05; **, p = 0.001–0.01; two-tailed Student’s t test. (E) Frequency of anaphase bridges positive for PICH and BLM, PICH only, or BLM only. One hundred anaphase bridges were analyzed for each sample.
Gfp Blm, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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pegfp blm  (Addgene inc)
90
Addgene inc pegfp blm
FIGURE 3: Depletion <t>of</t> <t>Smc5</t> or Smc6 disrupts chromosome segregation. (A) Example images of the anaphase bridges (red arrowheads) and lagging chromosomes (green arrowheads) frequently seen in Smc5/6-depleted cells. Scale bar: 10 μm. (B) Examples of the formation of micronuclei in Smc5- and Smc6-depleted cells. Green arrows indicate micronuclei containing the centromere marker CREST; red arrows indicate micronuclei negative for the CREST signal. Scale bar: 10 μm. (C) Representative images of PICH (red) and <t>BLM</t> (green) immunofluorescence in anaphase bridges. DNA was counterstained with DAPI (blue). Scale bar: 10 μm. (D) Frequency of anaphase bridges and lagging chromosomes within the anaphase chromosomes observed in the control and siRNA-treated cells. Three hundred anaphases were analyzed per condition. Bar graph shows mean ± SD from three independent experiments; p value < 0.05; **, p = 0.001–0.01; two-tailed Student’s t test. (E) Frequency of anaphase bridges positive for PICH and BLM, PICH only, or BLM only. One hundred anaphase bridges were analyzed for each sample.
Pegfp Blm, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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blm  (Cayman Chemical)
94
Cayman Chemical blm
FIGURE 3: Depletion <t>of</t> <t>Smc5</t> or Smc6 disrupts chromosome segregation. (A) Example images of the anaphase bridges (red arrowheads) and lagging chromosomes (green arrowheads) frequently seen in Smc5/6-depleted cells. Scale bar: 10 μm. (B) Examples of the formation of micronuclei in Smc5- and Smc6-depleted cells. Green arrows indicate micronuclei containing the centromere marker CREST; red arrows indicate micronuclei negative for the CREST signal. Scale bar: 10 μm. (C) Representative images of PICH (red) and <t>BLM</t> (green) immunofluorescence in anaphase bridges. DNA was counterstained with DAPI (blue). Scale bar: 10 μm. (D) Frequency of anaphase bridges and lagging chromosomes within the anaphase chromosomes observed in the control and siRNA-treated cells. Three hundred anaphases were analyzed per condition. Bar graph shows mean ± SD from three independent experiments; p value < 0.05; **, p = 0.001–0.01; two-tailed Student’s t test. (E) Frequency of anaphase bridges positive for PICH and BLM, PICH only, or BLM only. One hundred anaphase bridges were analyzed for each sample.
Blm, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
blm - by Bioz Stars, 2026-03
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anti blm antibody  (Novus Biologicals)
92
Novus Biologicals anti blm antibody
FIGURE 3: Depletion <t>of</t> <t>Smc5</t> or Smc6 disrupts chromosome segregation. (A) Example images of the anaphase bridges (red arrowheads) and lagging chromosomes (green arrowheads) frequently seen in Smc5/6-depleted cells. Scale bar: 10 μm. (B) Examples of the formation of micronuclei in Smc5- and Smc6-depleted cells. Green arrows indicate micronuclei containing the centromere marker CREST; red arrows indicate micronuclei negative for the CREST signal. Scale bar: 10 μm. (C) Representative images of PICH (red) and <t>BLM</t> (green) immunofluorescence in anaphase bridges. DNA was counterstained with DAPI (blue). Scale bar: 10 μm. (D) Frequency of anaphase bridges and lagging chromosomes within the anaphase chromosomes observed in the control and siRNA-treated cells. Three hundred anaphases were analyzed per condition. Bar graph shows mean ± SD from three independent experiments; p value < 0.05; **, p = 0.001–0.01; two-tailed Student’s t test. (E) Frequency of anaphase bridges positive for PICH and BLM, PICH only, or BLM only. One hundred anaphase bridges were analyzed for each sample.
Anti Blm Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
anti blm antibody - by Bioz Stars, 2026-03
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gene exp blm hs01119891 m1  (Thermo Fisher)
93
Thermo Fisher gene exp blm hs01119891 m1
FIGURE 3: Depletion <t>of</t> <t>Smc5</t> or Smc6 disrupts chromosome segregation. (A) Example images of the anaphase bridges (red arrowheads) and lagging chromosomes (green arrowheads) frequently seen in Smc5/6-depleted cells. Scale bar: 10 μm. (B) Examples of the formation of micronuclei in Smc5- and Smc6-depleted cells. Green arrows indicate micronuclei containing the centromere marker CREST; red arrows indicate micronuclei negative for the CREST signal. Scale bar: 10 μm. (C) Representative images of PICH (red) and <t>BLM</t> (green) immunofluorescence in anaphase bridges. DNA was counterstained with DAPI (blue). Scale bar: 10 μm. (D) Frequency of anaphase bridges and lagging chromosomes within the anaphase chromosomes observed in the control and siRNA-treated cells. Three hundred anaphases were analyzed per condition. Bar graph shows mean ± SD from three independent experiments; p value < 0.05; **, p = 0.001–0.01; two-tailed Student’s t test. (E) Frequency of anaphase bridges positive for PICH and BLM, PICH only, or BLM only. One hundred anaphase bridges were analyzed for each sample.
Gene Exp Blm Hs01119891 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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plasmids 80070  (Addgene inc)
90
Addgene inc plasmids 80070
FIGURE 3: Depletion <t>of</t> <t>Smc5</t> or Smc6 disrupts chromosome segregation. (A) Example images of the anaphase bridges (red arrowheads) and lagging chromosomes (green arrowheads) frequently seen in Smc5/6-depleted cells. Scale bar: 10 μm. (B) Examples of the formation of micronuclei in Smc5- and Smc6-depleted cells. Green arrows indicate micronuclei containing the centromere marker CREST; red arrows indicate micronuclei negative for the CREST signal. Scale bar: 10 μm. (C) Representative images of PICH (red) and <t>BLM</t> (green) immunofluorescence in anaphase bridges. DNA was counterstained with DAPI (blue). Scale bar: 10 μm. (D) Frequency of anaphase bridges and lagging chromosomes within the anaphase chromosomes observed in the control and siRNA-treated cells. Three hundred anaphases were analyzed per condition. Bar graph shows mean ± SD from three independent experiments; p value < 0.05; **, p = 0.001–0.01; two-tailed Student’s t test. (E) Frequency of anaphase bridges positive for PICH and BLM, PICH only, or BLM only. One hundred anaphase bridges were analyzed for each sample.
Plasmids 80070, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Image Search Results


FIGURE 3: Depletion of Smc5 or Smc6 disrupts chromosome segregation. (A) Example images of the anaphase bridges (red arrowheads) and lagging chromosomes (green arrowheads) frequently seen in Smc5/6-depleted cells. Scale bar: 10 μm. (B) Examples of the formation of micronuclei in Smc5- and Smc6-depleted cells. Green arrows indicate micronuclei containing the centromere marker CREST; red arrows indicate micronuclei negative for the CREST signal. Scale bar: 10 μm. (C) Representative images of PICH (red) and BLM (green) immunofluorescence in anaphase bridges. DNA was counterstained with DAPI (blue). Scale bar: 10 μm. (D) Frequency of anaphase bridges and lagging chromosomes within the anaphase chromosomes observed in the control and siRNA-treated cells. Three hundred anaphases were analyzed per condition. Bar graph shows mean ± SD from three independent experiments; p value < 0.05; **, p = 0.001–0.01; two-tailed Student’s t test. (E) Frequency of anaphase bridges positive for PICH and BLM, PICH only, or BLM only. One hundred anaphase bridges were analyzed for each sample.

Journal: Molecular Biology of the Cell

Article Title: Smc5/6-mediated regulation of replication progression contributes to chromosome assembly during mitosis in human cells

doi: 10.1091/mbc.e13-01-0020

Figure Lengend Snippet: FIGURE 3: Depletion of Smc5 or Smc6 disrupts chromosome segregation. (A) Example images of the anaphase bridges (red arrowheads) and lagging chromosomes (green arrowheads) frequently seen in Smc5/6-depleted cells. Scale bar: 10 μm. (B) Examples of the formation of micronuclei in Smc5- and Smc6-depleted cells. Green arrows indicate micronuclei containing the centromere marker CREST; red arrows indicate micronuclei negative for the CREST signal. Scale bar: 10 μm. (C) Representative images of PICH (red) and BLM (green) immunofluorescence in anaphase bridges. DNA was counterstained with DAPI (blue). Scale bar: 10 μm. (D) Frequency of anaphase bridges and lagging chromosomes within the anaphase chromosomes observed in the control and siRNA-treated cells. Three hundred anaphases were analyzed per condition. Bar graph shows mean ± SD from three independent experiments; p value < 0.05; **, p = 0.001–0.01; two-tailed Student’s t test. (E) Frequency of anaphase bridges positive for PICH and BLM, PICH only, or BLM only. One hundred anaphase bridges were analyzed for each sample.

Article Snippet: Antibodies against other proteins were as follows: SMC6L1 (M01, Cl7one 2E6; Abnova, Taipei, Taiwan), Smc6 (sc-365742, clone A-3; Santa Cruz Biotechnology, Santa Cruz, CA), SMC5 (18038; Abcam, Cambridge, UK), BLM (sc-7790, Santa Cruz Biotechnology), PICH (Clone 142-26-3; Millipore, Billerica, MA), DNA topoisomerase IIα (D081-1, clone 8D2; MBL, Aichi, Japan), DNA topoisomerase IIα (74715; Abcam), cyclin B (610219; BD PharMingen, Lexington, KY), α-tubulin (T6074, clone B-5-1-2; Sigma-Aldrich, St. Louis, MO), GFP (290; Abcam), and phospho– histone H3 (Ser-10) (9701; Cell Signaling Technology, Danvers, MA), cyclin B1 (K0128-3, clone V152; MBL), phospho–histone H3 (Ser-10) (9706, clone 6G3; Cell Signaling Technology), histone H2B (ab1790; Abcam), and γ-H2AX (A300-081A; Bethyl, Montgomery, TX).

Techniques: Marker, Immunofluorescence, Control, Two Tailed Test

FIGURE 8: Replication-related DNA damage. RPE-1 cells transfected with an siRNA against Smc5 or Smc6 or a control mock were analyzed at the specified time points after release from serum starvation. (A) Representative immunofluorescence of γH2AX (red) and BLM (green) foci formation in control cells (top panels) and in cells depleted of Smc6 (bottom panels). DAPI staining is shown in blue. A positive control for γH2AX and BLM staining is provided by low doze of aphidicolin treatment (aph 0.4 μM; middle panels). Scale bar: 10 μm. (B) Frequency of γH2AX-positive cells at indicated time points after the release from serum starvation. (C) Frequency of γH2AX-positive cells at the 33-h time point, with or without additional RO-3306 treatment for 12 h, and further classified according to the number of foci per cell as indicated (n > 31 per condition). Pink bars denote means of foci number per cell. (D) Giemsa-stained spread chromosomes from cells depleted of Smc6 or mock (control) were evaluated for their morphology and are summarized in the histogram. Three hundred cells were examined for each experiment. (E) Frequency of curly axial staining of topo IIα in Smc5/6-depleted cells in the presence or absence of RO-3306 for an additional 12 h. Bar graph shows mean ± SD from three independent experiments; p value < 0.05; *, p = 0.01–0.05; **, p = 0.001–0.01; two-tailed Student’s t test.

Journal: Molecular Biology of the Cell

Article Title: Smc5/6-mediated regulation of replication progression contributes to chromosome assembly during mitosis in human cells

doi: 10.1091/mbc.e13-01-0020

Figure Lengend Snippet: FIGURE 8: Replication-related DNA damage. RPE-1 cells transfected with an siRNA against Smc5 or Smc6 or a control mock were analyzed at the specified time points after release from serum starvation. (A) Representative immunofluorescence of γH2AX (red) and BLM (green) foci formation in control cells (top panels) and in cells depleted of Smc6 (bottom panels). DAPI staining is shown in blue. A positive control for γH2AX and BLM staining is provided by low doze of aphidicolin treatment (aph 0.4 μM; middle panels). Scale bar: 10 μm. (B) Frequency of γH2AX-positive cells at indicated time points after the release from serum starvation. (C) Frequency of γH2AX-positive cells at the 33-h time point, with or without additional RO-3306 treatment for 12 h, and further classified according to the number of foci per cell as indicated (n > 31 per condition). Pink bars denote means of foci number per cell. (D) Giemsa-stained spread chromosomes from cells depleted of Smc6 or mock (control) were evaluated for their morphology and are summarized in the histogram. Three hundred cells were examined for each experiment. (E) Frequency of curly axial staining of topo IIα in Smc5/6-depleted cells in the presence or absence of RO-3306 for an additional 12 h. Bar graph shows mean ± SD from three independent experiments; p value < 0.05; *, p = 0.01–0.05; **, p = 0.001–0.01; two-tailed Student’s t test.

Article Snippet: Antibodies against other proteins were as follows: SMC6L1 (M01, Cl7one 2E6; Abnova, Taipei, Taiwan), Smc6 (sc-365742, clone A-3; Santa Cruz Biotechnology, Santa Cruz, CA), SMC5 (18038; Abcam, Cambridge, UK), BLM (sc-7790, Santa Cruz Biotechnology), PICH (Clone 142-26-3; Millipore, Billerica, MA), DNA topoisomerase IIα (D081-1, clone 8D2; MBL, Aichi, Japan), DNA topoisomerase IIα (74715; Abcam), cyclin B (610219; BD PharMingen, Lexington, KY), α-tubulin (T6074, clone B-5-1-2; Sigma-Aldrich, St. Louis, MO), GFP (290; Abcam), and phospho– histone H3 (Ser-10) (9701; Cell Signaling Technology, Danvers, MA), cyclin B1 (K0128-3, clone V152; MBL), phospho–histone H3 (Ser-10) (9706, clone 6G3; Cell Signaling Technology), histone H2B (ab1790; Abcam), and γ-H2AX (A300-081A; Bethyl, Montgomery, TX).

Techniques: Transfection, Control, Immunofluorescence, Staining, Positive Control, Two Tailed Test