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blm helicase inhibition  (MedChemExpress)


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    Structured Review

    MedChemExpress blm helicase inhibition
    Blm Helicase Inhibition, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blm/BLM-IN-1/pm41990564-29-1-8
    Average 94 stars, based on 3 article reviews
    blm helicase inhibition - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Inhibition:

    Article Title: Characterization of post-mitotic DNA synthesis and its regulation in human cells
    Article Snippet: .. For replication stress induction, cells were treated with 1 μM ATR inhibitor AZ-20 (ATRi, Tocris Bioscience, 5198) for 24 hours; 0.2 μM Aphidicolin (APH, Sigma-Aldrich, A0781) for 24 hours; or the combination of 1 μM AZ-20 and 0.2 μM APH for 24 h. For acute inhibition, cells were treated with 1 μM SUMO inhibitor ML-792 (SUMOi, Selleckchem, S8697) for 2 h; 20 μM ATR inhibitor VE-821 (ATRi, Sigma-Aldrich, SML1415) or AZ-20 (ATRi, Tocris Bioscience, 5198) for 2 h; 2 μM BLM inhibitor BLM-IN-1 (BLMi, Med Chem Express, HY- 111756) for 2 h; or 1 μM ubiquitin E1 inhibitor TAK-243 (E1i, Med Chem Express, HY-100487) for 4 h. Cloning of pcDNA3.1(+)-TurboID-RPA32-HA plasmid To generate the pcDNA3.1(+)-TurboID-RPA32-HA construct, mCherryCry2WT was deleted from the pcDNA5-TurboID-RPA2-mCherryCry2WT plasmid94 and replaced with an HA.1 tag using primers TurboID-RPA2_mCh_Cry2_del_HA-stop_fw and TurboID-RPA2_mCh_Cry2_del_rev, followed by blunt-end ligation. ..

    Article Title: BLM helicase deficiency impairs porcine oocyte maturation via induction of organelle dysfunction.
    Article Snippet: BLM helicase plays a well-established role in safeguarding genomic integrity and cellular homeostasis by regulating DNA replication, transcription, and repair.. However, its function during meiotic maturation in porcine oocytes remains largely unexplored.. Here, we first delineated the subcellular localization of BLM helicase at key stages of porcine oocyte meiotic progression.

    Ubiquitin Proteomics:

    Article Title: Characterization of post-mitotic DNA synthesis and its regulation in human cells
    Article Snippet: .. For replication stress induction, cells were treated with 1 μM ATR inhibitor AZ-20 (ATRi, Tocris Bioscience, 5198) for 24 hours; 0.2 μM Aphidicolin (APH, Sigma-Aldrich, A0781) for 24 hours; or the combination of 1 μM AZ-20 and 0.2 μM APH for 24 h. For acute inhibition, cells were treated with 1 μM SUMO inhibitor ML-792 (SUMOi, Selleckchem, S8697) for 2 h; 20 μM ATR inhibitor VE-821 (ATRi, Sigma-Aldrich, SML1415) or AZ-20 (ATRi, Tocris Bioscience, 5198) for 2 h; 2 μM BLM inhibitor BLM-IN-1 (BLMi, Med Chem Express, HY- 111756) for 2 h; or 1 μM ubiquitin E1 inhibitor TAK-243 (E1i, Med Chem Express, HY-100487) for 4 h. Cloning of pcDNA3.1(+)-TurboID-RPA32-HA plasmid To generate the pcDNA3.1(+)-TurboID-RPA32-HA construct, mCherryCry2WT was deleted from the pcDNA5-TurboID-RPA2-mCherryCry2WT plasmid94 and replaced with an HA.1 tag using primers TurboID-RPA2_mCh_Cry2_del_HA-stop_fw and TurboID-RPA2_mCh_Cry2_del_rev, followed by blunt-end ligation. ..

    Cloning:

    Article Title: Characterization of post-mitotic DNA synthesis and its regulation in human cells
    Article Snippet: .. For replication stress induction, cells were treated with 1 μM ATR inhibitor AZ-20 (ATRi, Tocris Bioscience, 5198) for 24 hours; 0.2 μM Aphidicolin (APH, Sigma-Aldrich, A0781) for 24 hours; or the combination of 1 μM AZ-20 and 0.2 μM APH for 24 h. For acute inhibition, cells were treated with 1 μM SUMO inhibitor ML-792 (SUMOi, Selleckchem, S8697) for 2 h; 20 μM ATR inhibitor VE-821 (ATRi, Sigma-Aldrich, SML1415) or AZ-20 (ATRi, Tocris Bioscience, 5198) for 2 h; 2 μM BLM inhibitor BLM-IN-1 (BLMi, Med Chem Express, HY- 111756) for 2 h; or 1 μM ubiquitin E1 inhibitor TAK-243 (E1i, Med Chem Express, HY-100487) for 4 h. Cloning of pcDNA3.1(+)-TurboID-RPA32-HA plasmid To generate the pcDNA3.1(+)-TurboID-RPA32-HA construct, mCherryCry2WT was deleted from the pcDNA5-TurboID-RPA2-mCherryCry2WT plasmid94 and replaced with an HA.1 tag using primers TurboID-RPA2_mCh_Cry2_del_HA-stop_fw and TurboID-RPA2_mCh_Cry2_del_rev, followed by blunt-end ligation. ..

    Plasmid Preparation:

    Article Title: Characterization of post-mitotic DNA synthesis and its regulation in human cells
    Article Snippet: .. For replication stress induction, cells were treated with 1 μM ATR inhibitor AZ-20 (ATRi, Tocris Bioscience, 5198) for 24 hours; 0.2 μM Aphidicolin (APH, Sigma-Aldrich, A0781) for 24 hours; or the combination of 1 μM AZ-20 and 0.2 μM APH for 24 h. For acute inhibition, cells were treated with 1 μM SUMO inhibitor ML-792 (SUMOi, Selleckchem, S8697) for 2 h; 20 μM ATR inhibitor VE-821 (ATRi, Sigma-Aldrich, SML1415) or AZ-20 (ATRi, Tocris Bioscience, 5198) for 2 h; 2 μM BLM inhibitor BLM-IN-1 (BLMi, Med Chem Express, HY- 111756) for 2 h; or 1 μM ubiquitin E1 inhibitor TAK-243 (E1i, Med Chem Express, HY-100487) for 4 h. Cloning of pcDNA3.1(+)-TurboID-RPA32-HA plasmid To generate the pcDNA3.1(+)-TurboID-RPA32-HA construct, mCherryCry2WT was deleted from the pcDNA5-TurboID-RPA2-mCherryCry2WT plasmid94 and replaced with an HA.1 tag using primers TurboID-RPA2_mCh_Cry2_del_HA-stop_fw and TurboID-RPA2_mCh_Cry2_del_rev, followed by blunt-end ligation. ..

    Construct:

    Article Title: Characterization of post-mitotic DNA synthesis and its regulation in human cells
    Article Snippet: .. For replication stress induction, cells were treated with 1 μM ATR inhibitor AZ-20 (ATRi, Tocris Bioscience, 5198) for 24 hours; 0.2 μM Aphidicolin (APH, Sigma-Aldrich, A0781) for 24 hours; or the combination of 1 μM AZ-20 and 0.2 μM APH for 24 h. For acute inhibition, cells were treated with 1 μM SUMO inhibitor ML-792 (SUMOi, Selleckchem, S8697) for 2 h; 20 μM ATR inhibitor VE-821 (ATRi, Sigma-Aldrich, SML1415) or AZ-20 (ATRi, Tocris Bioscience, 5198) for 2 h; 2 μM BLM inhibitor BLM-IN-1 (BLMi, Med Chem Express, HY- 111756) for 2 h; or 1 μM ubiquitin E1 inhibitor TAK-243 (E1i, Med Chem Express, HY-100487) for 4 h. Cloning of pcDNA3.1(+)-TurboID-RPA32-HA plasmid To generate the pcDNA3.1(+)-TurboID-RPA32-HA construct, mCherryCry2WT was deleted from the pcDNA5-TurboID-RPA2-mCherryCry2WT plasmid94 and replaced with an HA.1 tag using primers TurboID-RPA2_mCh_Cry2_del_HA-stop_fw and TurboID-RPA2_mCh_Cry2_del_rev, followed by blunt-end ligation. ..

    Hemagglutination Assay:

    Article Title: Characterization of post-mitotic DNA synthesis and its regulation in human cells
    Article Snippet: .. For replication stress induction, cells were treated with 1 μM ATR inhibitor AZ-20 (ATRi, Tocris Bioscience, 5198) for 24 hours; 0.2 μM Aphidicolin (APH, Sigma-Aldrich, A0781) for 24 hours; or the combination of 1 μM AZ-20 and 0.2 μM APH for 24 h. For acute inhibition, cells were treated with 1 μM SUMO inhibitor ML-792 (SUMOi, Selleckchem, S8697) for 2 h; 20 μM ATR inhibitor VE-821 (ATRi, Sigma-Aldrich, SML1415) or AZ-20 (ATRi, Tocris Bioscience, 5198) for 2 h; 2 μM BLM inhibitor BLM-IN-1 (BLMi, Med Chem Express, HY- 111756) for 2 h; or 1 μM ubiquitin E1 inhibitor TAK-243 (E1i, Med Chem Express, HY-100487) for 4 h. Cloning of pcDNA3.1(+)-TurboID-RPA32-HA plasmid To generate the pcDNA3.1(+)-TurboID-RPA32-HA construct, mCherryCry2WT was deleted from the pcDNA5-TurboID-RPA2-mCherryCry2WT plasmid94 and replaced with an HA.1 tag using primers TurboID-RPA2_mCh_Cry2_del_HA-stop_fw and TurboID-RPA2_mCh_Cry2_del_rev, followed by blunt-end ligation. ..

    Ligation:

    Article Title: Characterization of post-mitotic DNA synthesis and its regulation in human cells
    Article Snippet: .. For replication stress induction, cells were treated with 1 μM ATR inhibitor AZ-20 (ATRi, Tocris Bioscience, 5198) for 24 hours; 0.2 μM Aphidicolin (APH, Sigma-Aldrich, A0781) for 24 hours; or the combination of 1 μM AZ-20 and 0.2 μM APH for 24 h. For acute inhibition, cells were treated with 1 μM SUMO inhibitor ML-792 (SUMOi, Selleckchem, S8697) for 2 h; 20 μM ATR inhibitor VE-821 (ATRi, Sigma-Aldrich, SML1415) or AZ-20 (ATRi, Tocris Bioscience, 5198) for 2 h; 2 μM BLM inhibitor BLM-IN-1 (BLMi, Med Chem Express, HY- 111756) for 2 h; or 1 μM ubiquitin E1 inhibitor TAK-243 (E1i, Med Chem Express, HY-100487) for 4 h. Cloning of pcDNA3.1(+)-TurboID-RPA32-HA plasmid To generate the pcDNA3.1(+)-TurboID-RPA32-HA construct, mCherryCry2WT was deleted from the pcDNA5-TurboID-RPA2-mCherryCry2WT plasmid94 and replaced with an HA.1 tag using primers TurboID-RPA2_mCh_Cry2_del_HA-stop_fw and TurboID-RPA2_mCh_Cry2_del_rev, followed by blunt-end ligation. ..

    Saline:

    Article Title: Elephantopus scaber L. Ethanolic Leaves Extract Modulates IL-2 Production and T-Lymphocyte Activation in Pulmonary Fibrosis Mice Model
    Article Snippet: The ethanol extract of E. scaber leaves will be dissolved in corn oil (Mazola, ACH Food Companies Inc., US) and given daily for 14 days by oral gavages. .. Bleomycin sulfate (MedChemExpress LLC, US) 10 mg was dissolved in 1 mL phosphate-buffered saline (PBS) and divided into propylene tubes containing 200 mL. ..



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