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annexin v-fitc and pi reagents in binding buffer using the annexin v-fitc/pi cell apoptosis detection kit  (Beyotime)


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    Beyotime annexin v-fitc and pi reagents in binding buffer using the annexin v-fitc/pi cell apoptosis detection kit
    Annexin V Fitc And Pi Reagents In Binding Buffer Using The Annexin V Fitc/Pi Cell Apoptosis Detection Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/binding+buffer+cell+apoptosis+kit/annexin+v+fitc+and+pi+reagents+in+binding+buffer+using+the+annexin+v+fitc+pi+cell+apoptosis+detection+kit/pmc04933872-32-32-48
    Average 90 stars, based on 1 article reviews
    annexin v-fitc and pi reagents in binding buffer using the annexin v-fitc/pi cell apoptosis detection kit - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: Upregulation of MicroRNA-214 Contributes to the Development of Vascular Remodeling in Hypoxia-induced Pulmonary Hypertension Via Targeting CCNL2
    Article Snippet: After 48 h, the cells treated under various conditions were incubated in 1.5% H 2 O 2 for 24 h. The cells were then starved in medium containing 0.1% FBS; stained with Annexin V-FITC and PI reagents in binding buffer using the Annexin V-FITC/PI cell apoptosis detection kit (Beyotime Institute of Biotechnology, Beijing, China); and analyzed using a FACSCalibur flow cytometer.

    Binding Assay:

    Article Title: miR-221 regulates proliferation and apoptosis of ovarian cancer cells by targeting BMF.
    Article Snippet: .. Cells were re-suspended in 100 μl binding buffer (part of the apoptosis detection kit), then cells were incubated with a cell apoptosis detection kit [10 μl (15 M) Annexin V dye (FITC) and 5 μl (15 M) PI dye; Beyotime Institute of Biotechnology, Haimen, China] in the dark for 15 min at 37 ̊C. .. Cell apoptosis was detected with a Beckman Coulter FC 500 MCL/MPL flow cytometer and analyzed using FCS Express 3.0 software (DeNovo software, Glendale, CA, USA).

    Incubation:

    Article Title: miR-221 regulates proliferation and apoptosis of ovarian cancer cells by targeting BMF.
    Article Snippet: .. Cells were re-suspended in 100 μl binding buffer (part of the apoptosis detection kit), then cells were incubated with a cell apoptosis detection kit [10 μl (15 M) Annexin V dye (FITC) and 5 μl (15 M) PI dye; Beyotime Institute of Biotechnology, Haimen, China] in the dark for 15 min at 37 ̊C. .. Cell apoptosis was detected with a Beckman Coulter FC 500 MCL/MPL flow cytometer and analyzed using FCS Express 3.0 software (DeNovo software, Glendale, CA, USA).

    Modification:

    Article Title: Long-Circulating and Targeted Liposomes Co-loading Cisplatin and Mifamurtide: Formulation and Delivery in Osteosarcoma Cells.
    Article Snippet: Osteosarcoma (OS) is one of the most common primary bone sarcoma with high malignant degree and poor prognosis, for which there is an urgent need to develop novel therapeutic approaches.. Recent research has revealed that mifamurtide significantly improved the outcome of OS patients when combined with adjuvant chemotherapy drugs including cisplatin (DDP).. The present study aimed to construct a drug delivery system co-loading DDP and mifamurtide.

    Double Staining:

    Article Title: Long-Circulating and Targeted Liposomes Co-loading Cisplatin and Mifamurtide: Formulation and Delivery in Osteosarcoma Cells.
    Article Snippet: Osteosarcoma (OS) is one of the most common primary bone sarcoma with high malignant degree and poor prognosis, for which there is an urgent need to develop novel therapeutic approaches.. Recent research has revealed that mifamurtide significantly improved the outcome of OS patients when combined with adjuvant chemotherapy drugs including cisplatin (DDP).. The present study aimed to construct a drug delivery system co-loading DDP and mifamurtide.



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    Image Search Results


    Effect of taurine on the apoptosis of human non-small cell lung cancer A549 cell line. *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Effect of taurine on the apoptosis of human non-small cell lung cancer A549 cell line. *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Control

    Hoechst 33342 staining for the effect of taurine on the apoptosis of cell line A549 (magnification, ×200). *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Hoechst 33342 staining for the effect of taurine on the apoptosis of cell line A549 (magnification, ×200). *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Staining, Control

    Effect of taurine on the expression of PUMA, Bax and Bcl-2 proteins in human lung cancer A549 cell line. Values are expressed as the mean ± standard deviation; n=3. *P<0.05 vs. control group. Tau, taurine; DDP, cisplatin; Bcl-2, BCL2 apoptosis regulator; Bax, BCL2 associated X, apoptosis regulator; PUMA, p53 upregulated modulator of apoptosis; IOD, integrated option density.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Effect of taurine on the expression of PUMA, Bax and Bcl-2 proteins in human lung cancer A549 cell line. Values are expressed as the mean ± standard deviation; n=3. *P<0.05 vs. control group. Tau, taurine; DDP, cisplatin; Bcl-2, BCL2 apoptosis regulator; Bax, BCL2 associated X, apoptosis regulator; PUMA, p53 upregulated modulator of apoptosis; IOD, integrated option density.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Expressing, Standard Deviation, Control

    Suppressive effect of taurine and PUMA expression on the growth of lung cancer A549 cell-derived xenograft tumors in nude mice. (A) The growth curve of tumor in nude mice bearing human non-small cell lung cancer A549 cell xenografts. (B) Tumors in nude mice bearing human non-small cell lung cancer A549 cells xenografts. (C) The tumor inhibition rate following 27 days of treatment. Values are expressed as the mean ± SD; n=5. **P<0.01 vs. negative or control; # P<0.05 vs. the PUMA or taurine group. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Suppressive effect of taurine and PUMA expression on the growth of lung cancer A549 cell-derived xenograft tumors in nude mice. (A) The growth curve of tumor in nude mice bearing human non-small cell lung cancer A549 cell xenografts. (B) Tumors in nude mice bearing human non-small cell lung cancer A549 cells xenografts. (C) The tumor inhibition rate following 27 days of treatment. Values are expressed as the mean ± SD; n=5. **P<0.01 vs. negative or control; # P<0.05 vs. the PUMA or taurine group. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Expressing, Derivative Assay, Inhibition, Control

    Taurine alters expression of PUMA in lung cancer A549 cell-derived xenograft tumors in nude mice (magnification, ×400). (A) Control; (B) negative; (C) exogenous PUMA; (D) taurine; (E) taurine and exogenous PUMA. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Taurine alters expression of PUMA in lung cancer A549 cell-derived xenograft tumors in nude mice (magnification, ×400). (A) Control; (B) negative; (C) exogenous PUMA; (D) taurine; (E) taurine and exogenous PUMA. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Expressing, Derivative Assay, Control

    Taurine alters the expression of Bcl-2 in A549 lung cancer cell-derived xenograft tumors in nude mice (magnification, ×400). (A) Control; (B) negative; (C) exogenous PUMA; (D) taurine; (E) taurine and exogenous PUMA. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis; Bcl-2, BCL2, apoptosis regulator.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Taurine alters the expression of Bcl-2 in A549 lung cancer cell-derived xenograft tumors in nude mice (magnification, ×400). (A) Control; (B) negative; (C) exogenous PUMA; (D) taurine; (E) taurine and exogenous PUMA. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis; Bcl-2, BCL2, apoptosis regulator.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Expressing, Derivative Assay, Control

    Effect of taurine on the expression of PUMA, Bax and Bcl-2 in A549 lung cancer cell-derived xenograft tumors in nude mice. **P<0.01 vs. control or negative; # P<0.01 vs. PUMA or taurine (mean ± standard deviation; n=5). Bcl-2, BCL2 apoptosis regulator; Bax, BCL2 associated X, apoptosis regulator; PUMA, p53 upregulated modulator of apoptosis.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Effect of taurine on the expression of PUMA, Bax and Bcl-2 in A549 lung cancer cell-derived xenograft tumors in nude mice. **P<0.01 vs. control or negative; # P<0.01 vs. PUMA or taurine (mean ± standard deviation; n=5). Bcl-2, BCL2 apoptosis regulator; Bax, BCL2 associated X, apoptosis regulator; PUMA, p53 upregulated modulator of apoptosis.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Expressing, Derivative Assay, Control, Standard Deviation