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binding buffer (annexin v-fitc/pi double staining cell apoptosis detection kit)  (Keygen Biotech)

 
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    Structured Review

    Keygen Biotech binding buffer (annexin v-fitc/pi double staining cell apoptosis detection kit)
    Effect of taurine on the <t>apoptosis</t> of human non-small cell lung cancer A549 cell line. *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.
    Binding Buffer (Annexin V Fitc/Pi Double Staining Cell Apoptosis Detection Kit), supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/binding+buffer+cell+apoptosis+kit/binding+buffer/pmc05840730-54-19-28
    Average 90 stars, based on 1 article reviews
    binding buffer (annexin v-fitc/pi double staining cell apoptosis detection kit) - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells"

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    Journal: Oncology Letters

    doi: 10.3892/ol.2018.8036

    Effect of taurine on the apoptosis of human non-small cell lung cancer A549 cell line. *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.
    Figure Legend Snippet: Effect of taurine on the apoptosis of human non-small cell lung cancer A549 cell line. *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.

    Techniques Used: Control

    Hoechst 33342 staining for the effect of taurine on the apoptosis of cell line A549 (magnification, ×200). *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.
    Figure Legend Snippet: Hoechst 33342 staining for the effect of taurine on the apoptosis of cell line A549 (magnification, ×200). *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.

    Techniques Used: Staining, Control

    Effect of taurine on the expression of PUMA, Bax and Bcl-2 proteins in human lung cancer A549 cell line. Values are expressed as the mean ± standard deviation; n=3. *P<0.05 vs. control group. Tau, taurine; DDP, cisplatin; Bcl-2, BCL2 apoptosis regulator; Bax, BCL2 associated X, apoptosis regulator; PUMA, p53 upregulated modulator of apoptosis; IOD, integrated option density.
    Figure Legend Snippet: Effect of taurine on the expression of PUMA, Bax and Bcl-2 proteins in human lung cancer A549 cell line. Values are expressed as the mean ± standard deviation; n=3. *P<0.05 vs. control group. Tau, taurine; DDP, cisplatin; Bcl-2, BCL2 apoptosis regulator; Bax, BCL2 associated X, apoptosis regulator; PUMA, p53 upregulated modulator of apoptosis; IOD, integrated option density.

    Techniques Used: Expressing, Standard Deviation, Control

    Suppressive effect of taurine and PUMA expression on the growth of lung cancer A549 cell-derived xenograft tumors in nude mice. (A) The growth curve of tumor in nude mice bearing human non-small cell lung cancer A549 cell xenografts. (B) Tumors in nude mice bearing human non-small cell lung cancer A549 cells xenografts. (C) The tumor inhibition rate following 27 days of treatment. Values are expressed as the mean ± SD; n=5. **P<0.01 vs. negative or control; # P<0.05 vs. the PUMA or taurine group. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis.
    Figure Legend Snippet: Suppressive effect of taurine and PUMA expression on the growth of lung cancer A549 cell-derived xenograft tumors in nude mice. (A) The growth curve of tumor in nude mice bearing human non-small cell lung cancer A549 cell xenografts. (B) Tumors in nude mice bearing human non-small cell lung cancer A549 cells xenografts. (C) The tumor inhibition rate following 27 days of treatment. Values are expressed as the mean ± SD; n=5. **P<0.01 vs. negative or control; # P<0.05 vs. the PUMA or taurine group. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis.

    Techniques Used: Expressing, Derivative Assay, Inhibition, Control

    Taurine alters expression of PUMA in lung cancer A549 cell-derived xenograft tumors in nude mice (magnification, ×400). (A) Control; (B) negative; (C) exogenous PUMA; (D) taurine; (E) taurine and exogenous PUMA. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis.
    Figure Legend Snippet: Taurine alters expression of PUMA in lung cancer A549 cell-derived xenograft tumors in nude mice (magnification, ×400). (A) Control; (B) negative; (C) exogenous PUMA; (D) taurine; (E) taurine and exogenous PUMA. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis.

    Techniques Used: Expressing, Derivative Assay, Control

    Taurine alters the expression of Bcl-2 in A549 lung cancer cell-derived xenograft tumors in nude mice (magnification, ×400). (A) Control; (B) negative; (C) exogenous PUMA; (D) taurine; (E) taurine and exogenous PUMA. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis; Bcl-2, BCL2, apoptosis regulator.
    Figure Legend Snippet: Taurine alters the expression of Bcl-2 in A549 lung cancer cell-derived xenograft tumors in nude mice (magnification, ×400). (A) Control; (B) negative; (C) exogenous PUMA; (D) taurine; (E) taurine and exogenous PUMA. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis; Bcl-2, BCL2, apoptosis regulator.

    Techniques Used: Expressing, Derivative Assay, Control

    Effect of taurine on the expression of PUMA, Bax and Bcl-2 in A549 lung cancer cell-derived xenograft tumors in nude mice. **P<0.01 vs. control or negative; # P<0.01 vs. PUMA or taurine (mean ± standard deviation; n=5). Bcl-2, BCL2 apoptosis regulator; Bax, BCL2 associated X, apoptosis regulator; PUMA, p53 upregulated modulator of apoptosis.
    Figure Legend Snippet: Effect of taurine on the expression of PUMA, Bax and Bcl-2 in A549 lung cancer cell-derived xenograft tumors in nude mice. **P<0.01 vs. control or negative; # P<0.01 vs. PUMA or taurine (mean ± standard deviation; n=5). Bcl-2, BCL2 apoptosis regulator; Bax, BCL2 associated X, apoptosis regulator; PUMA, p53 upregulated modulator of apoptosis.

    Techniques Used: Expressing, Derivative Assay, Control, Standard Deviation

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    Binding Assay:

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    Article Snippet: .. After washing, the cells were re-suspended using a binding buffer (KeyGen Biotech, Nanjing, China). ..

    Article Title: Network Pharmacological Prediction and Experimental Analyses Reveal That Naringin Alleviates Osteoarthritis Progression by Targeting MMP13
    Article Snippet: .. After washing, the cells were re-suspended using a binding buffer (KeyGen Biotech, Nanjing, China). ..

    Article Title: Ginsenoside Rg1 ameliorates renal ischemia-reperfusion injury by inhibiting FABP1-regulated Nrf2/HO-1 pathway
    Article Snippet: after incubating the cells for 24 h, the medium was refreshed, and 10 μL of CCK-8 reagent (biosharp, Guangzhou, China) was added to each well. the plates were kept in the dark for incubation, and cell viability was determined by measuring the absorbance at 450 nm using a microplate reader (ELx800, biotek, uSa). .. Cells and tissue samples were gathered, spun at 1000 r/min for 5 min, then resuspended in 100 μL of PbS, followed by the addition of 500 μL of 1 × annexin V binding buffer (KeyGEN, Nanjing, China), and add 5 μL of annexin VFItC (KeyGEN, Nanjing, China), mix gently, and incubate for 15 min at room temperature under the condition of avoiding light, add 5 μL of propidium iodide (PI, KeyGEN, Nanjing, China), and store the samples on ice, away from light, and then use a Cytoflex flow cytometer (beckman Coulter, uSa) to identify apoptosis within 60 min. ..

    Article Title: Exploration of the pharmacological mechanism of Evodia rutaecarpa on esophageal cancer based on comprehensive analysis of compounds and disease targets.
    Article Snippet: Fixed cells were centrifuged, the supernatant discarded, and washed with PBS before staining with propidium iodide (PI) using the Cell Cycle and Apoptosis Analysis Kit (Beyotime, C1052, China), which contains RNase A. .. The cells were then resuspended in the binding buffer from the Annexin V-FITC/PI Apoptosis Detection Kit (KeyGen, KGA1102, China), stained with Annexin V-FITC and PI, and incubated at room temperature in the dark for 15 min. Apoptosis was analyzed using a CaleCyte V1.0 flow cytometer (Kailer, China). ..

    Article Title: Targeting Retinoic Acid Receptor-Related Orphan Receptor α to Prevent Cataracts: A New Pathway Through Nerve Growth Factor Activation.
    Article Snippet: Aims: Cataracts are the leading cause of blindness worldwide, and the mechanism underlying cataract formation is linked to the oxidative damage and the apoptosis of lens epithelial cells.. Retinoic acid Receptor-related orphan receptor a (RORa), a transcription factor, prevents oxidative stress and cell apoptosis.. RORa is decreased in the lens from patients with cataract, but it remains unclear whether decreases in RORa are attributed to cataract formation.

    Article Title: Licochalcone B suppresses oxidative stress and apoptosis accompanied by upregulating Nrf2/HO-1 pathway to ameliorate diabetic nephropathy in mice
    Article Snippet: .. Cells were resuspended with 500 μL Binding Buffer (KeyGEN, Nanjing, China). .. Afterwards, cells were stained with 5 μL Annexin V–FITC and 5 μL Propidium iodide (KeyGEN, Jiangsu, China) for 15 min at room temperature in darkness.

    Article Title: Ginsenoside Rg1 ameliorates renal ischemia-reperfusion injury by inhibiting FABP1-regulated Nrf2/HO-1 pathway
    Article Snippet: .. Cells and tissue samples were gathered, spun at 1000 r/min for 5 min, then resuspended in 100 μL of PBS, followed by the addition of 500 μL of 1 × Annexin V Binding Buffer (KeyGEN, Nanjing, China), and add 5 μL of Annexin VFITC (KeyGEN, Nanjing, China), mix gently, and incubate for 15 min at room temperature under the condition of avoiding light, add 5 μL of propidium iodide (PI, KeyGEN, Nanjing, China), and store the samples on ice, away from light, and then use a Cytoflex flow cytometer (Beckman Coulter, USA) to identify apoptosis within 60 min. ..

    Article Title: Methyltransferase complex subunit METTL3 maintains genome stability of erythroid cells via MTHFD1-mediated nucleotide biosynthesis
    Article Snippet: .. After 2 washes, the cell pellet was resuspended in 200 μL binding buffer (KeyGEN BioTECH, KGA1102-100). ..

    Flow Cytometry:

    Article Title: Ginsenoside Rg1 ameliorates renal ischemia-reperfusion injury by inhibiting FABP1-regulated Nrf2/HO-1 pathway
    Article Snippet: after incubating the cells for 24 h, the medium was refreshed, and 10 μL of CCK-8 reagent (biosharp, Guangzhou, China) was added to each well. the plates were kept in the dark for incubation, and cell viability was determined by measuring the absorbance at 450 nm using a microplate reader (ELx800, biotek, uSa). .. Cells and tissue samples were gathered, spun at 1000 r/min for 5 min, then resuspended in 100 μL of PbS, followed by the addition of 500 μL of 1 × annexin V binding buffer (KeyGEN, Nanjing, China), and add 5 μL of annexin VFItC (KeyGEN, Nanjing, China), mix gently, and incubate for 15 min at room temperature under the condition of avoiding light, add 5 μL of propidium iodide (PI, KeyGEN, Nanjing, China), and store the samples on ice, away from light, and then use a Cytoflex flow cytometer (beckman Coulter, uSa) to identify apoptosis within 60 min. ..

    Article Title: Exploration of the pharmacological mechanism of Evodia rutaecarpa on esophageal cancer based on comprehensive analysis of compounds and disease targets.
    Article Snippet: Fixed cells were centrifuged, the supernatant discarded, and washed with PBS before staining with propidium iodide (PI) using the Cell Cycle and Apoptosis Analysis Kit (Beyotime, C1052, China), which contains RNase A. .. The cells were then resuspended in the binding buffer from the Annexin V-FITC/PI Apoptosis Detection Kit (KeyGen, KGA1102, China), stained with Annexin V-FITC and PI, and incubated at room temperature in the dark for 15 min. Apoptosis was analyzed using a CaleCyte V1.0 flow cytometer (Kailer, China). ..

    Article Title: Targeting Retinoic Acid Receptor-Related Orphan Receptor α to Prevent Cataracts: A New Pathway Through Nerve Growth Factor Activation.
    Article Snippet: Aims: Cataracts are the leading cause of blindness worldwide, and the mechanism underlying cataract formation is linked to the oxidative damage and the apoptosis of lens epithelial cells.. Retinoic acid Receptor-related orphan receptor a (RORa), a transcription factor, prevents oxidative stress and cell apoptosis.. RORa is decreased in the lens from patients with cataract, but it remains unclear whether decreases in RORa are attributed to cataract formation.

    Article Title: Ginsenoside Rg1 ameliorates renal ischemia-reperfusion injury by inhibiting FABP1-regulated Nrf2/HO-1 pathway
    Article Snippet: .. Cells and tissue samples were gathered, spun at 1000 r/min for 5 min, then resuspended in 100 μL of PBS, followed by the addition of 500 μL of 1 × Annexin V Binding Buffer (KeyGEN, Nanjing, China), and add 5 μL of Annexin VFITC (KeyGEN, Nanjing, China), mix gently, and incubate for 15 min at room temperature under the condition of avoiding light, add 5 μL of propidium iodide (PI, KeyGEN, Nanjing, China), and store the samples on ice, away from light, and then use a Cytoflex flow cytometer (Beckman Coulter, USA) to identify apoptosis within 60 min. ..

    Staining:

    Article Title: Exploration of the pharmacological mechanism of Evodia rutaecarpa on esophageal cancer based on comprehensive analysis of compounds and disease targets.
    Article Snippet: Fixed cells were centrifuged, the supernatant discarded, and washed with PBS before staining with propidium iodide (PI) using the Cell Cycle and Apoptosis Analysis Kit (Beyotime, C1052, China), which contains RNase A. .. The cells were then resuspended in the binding buffer from the Annexin V-FITC/PI Apoptosis Detection Kit (KeyGen, KGA1102, China), stained with Annexin V-FITC and PI, and incubated at room temperature in the dark for 15 min. Apoptosis was analyzed using a CaleCyte V1.0 flow cytometer (Kailer, China). ..

    Incubation:

    Article Title: Exploration of the pharmacological mechanism of Evodia rutaecarpa on esophageal cancer based on comprehensive analysis of compounds and disease targets.
    Article Snippet: Fixed cells were centrifuged, the supernatant discarded, and washed with PBS before staining with propidium iodide (PI) using the Cell Cycle and Apoptosis Analysis Kit (Beyotime, C1052, China), which contains RNase A. .. The cells were then resuspended in the binding buffer from the Annexin V-FITC/PI Apoptosis Detection Kit (KeyGen, KGA1102, China), stained with Annexin V-FITC and PI, and incubated at room temperature in the dark for 15 min. Apoptosis was analyzed using a CaleCyte V1.0 flow cytometer (Kailer, China). ..

    Article Title: Targeting Retinoic Acid Receptor-Related Orphan Receptor α to Prevent Cataracts: A New Pathway Through Nerve Growth Factor Activation.
    Article Snippet: Aims: Cataracts are the leading cause of blindness worldwide, and the mechanism underlying cataract formation is linked to the oxidative damage and the apoptosis of lens epithelial cells.. Retinoic acid Receptor-related orphan receptor a (RORa), a transcription factor, prevents oxidative stress and cell apoptosis.. RORa is decreased in the lens from patients with cataract, but it remains unclear whether decreases in RORa are attributed to cataract formation.



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    Image Search Results


    Effect of taurine on the apoptosis of human non-small cell lung cancer A549 cell line. *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Effect of taurine on the apoptosis of human non-small cell lung cancer A549 cell line. *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Control

    Hoechst 33342 staining for the effect of taurine on the apoptosis of cell line A549 (magnification, ×200). *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Hoechst 33342 staining for the effect of taurine on the apoptosis of cell line A549 (magnification, ×200). *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Staining, Control

    Effect of taurine on the expression of PUMA, Bax and Bcl-2 proteins in human lung cancer A549 cell line. Values are expressed as the mean ± standard deviation; n=3. *P<0.05 vs. control group. Tau, taurine; DDP, cisplatin; Bcl-2, BCL2 apoptosis regulator; Bax, BCL2 associated X, apoptosis regulator; PUMA, p53 upregulated modulator of apoptosis; IOD, integrated option density.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Effect of taurine on the expression of PUMA, Bax and Bcl-2 proteins in human lung cancer A549 cell line. Values are expressed as the mean ± standard deviation; n=3. *P<0.05 vs. control group. Tau, taurine; DDP, cisplatin; Bcl-2, BCL2 apoptosis regulator; Bax, BCL2 associated X, apoptosis regulator; PUMA, p53 upregulated modulator of apoptosis; IOD, integrated option density.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Expressing, Standard Deviation, Control

    Suppressive effect of taurine and PUMA expression on the growth of lung cancer A549 cell-derived xenograft tumors in nude mice. (A) The growth curve of tumor in nude mice bearing human non-small cell lung cancer A549 cell xenografts. (B) Tumors in nude mice bearing human non-small cell lung cancer A549 cells xenografts. (C) The tumor inhibition rate following 27 days of treatment. Values are expressed as the mean ± SD; n=5. **P<0.01 vs. negative or control; # P<0.05 vs. the PUMA or taurine group. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Suppressive effect of taurine and PUMA expression on the growth of lung cancer A549 cell-derived xenograft tumors in nude mice. (A) The growth curve of tumor in nude mice bearing human non-small cell lung cancer A549 cell xenografts. (B) Tumors in nude mice bearing human non-small cell lung cancer A549 cells xenografts. (C) The tumor inhibition rate following 27 days of treatment. Values are expressed as the mean ± SD; n=5. **P<0.01 vs. negative or control; # P<0.05 vs. the PUMA or taurine group. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Expressing, Derivative Assay, Inhibition, Control

    Taurine alters expression of PUMA in lung cancer A549 cell-derived xenograft tumors in nude mice (magnification, ×400). (A) Control; (B) negative; (C) exogenous PUMA; (D) taurine; (E) taurine and exogenous PUMA. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Taurine alters expression of PUMA in lung cancer A549 cell-derived xenograft tumors in nude mice (magnification, ×400). (A) Control; (B) negative; (C) exogenous PUMA; (D) taurine; (E) taurine and exogenous PUMA. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Expressing, Derivative Assay, Control

    Taurine alters the expression of Bcl-2 in A549 lung cancer cell-derived xenograft tumors in nude mice (magnification, ×400). (A) Control; (B) negative; (C) exogenous PUMA; (D) taurine; (E) taurine and exogenous PUMA. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis; Bcl-2, BCL2, apoptosis regulator.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Taurine alters the expression of Bcl-2 in A549 lung cancer cell-derived xenograft tumors in nude mice (magnification, ×400). (A) Control; (B) negative; (C) exogenous PUMA; (D) taurine; (E) taurine and exogenous PUMA. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis; Bcl-2, BCL2, apoptosis regulator.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Expressing, Derivative Assay, Control

    Effect of taurine on the expression of PUMA, Bax and Bcl-2 in A549 lung cancer cell-derived xenograft tumors in nude mice. **P<0.01 vs. control or negative; # P<0.01 vs. PUMA or taurine (mean ± standard deviation; n=5). Bcl-2, BCL2 apoptosis regulator; Bax, BCL2 associated X, apoptosis regulator; PUMA, p53 upregulated modulator of apoptosis.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Effect of taurine on the expression of PUMA, Bax and Bcl-2 in A549 lung cancer cell-derived xenograft tumors in nude mice. **P<0.01 vs. control or negative; # P<0.01 vs. PUMA or taurine (mean ± standard deviation; n=5). Bcl-2, BCL2 apoptosis regulator; Bax, BCL2 associated X, apoptosis regulator; PUMA, p53 upregulated modulator of apoptosis.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Expressing, Derivative Assay, Control, Standard Deviation