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Microzone Corp sequence enhance/dilution buffer better buffer
Sequence Enhance/Dilution Buffer Better Buffer, supplied by Microzone Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/better+buffer+sequencing+buffer/better+buffer/pm15885500-196-24-29
Average 90 stars, based on 1 article reviews
sequence enhance/dilution buffer better buffer - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Amplification:

Article Title: Genetic portrait of Lisboa immigrant population from Cabo Verde with mitochondrial DNA analysis.
Article Snippet: The amplified products were purified with ExoSAPIT (USB Corporation, Ohio, USA) method. .. The light and heavy chains from each of the two fragments amplified from the mtDNA control region were sequenced in a final volume of 8 μL containing Better Buffer (Microzone, Sussex, UK), Big Dye Terminator ver. .. 3.1 Cycle Sequence (ABI, Foster City, USA) and primers at 2.5 μM, in a GeneAmp PCR system 9700 (ABI, Foster City, USA) thermo cycler.

Article Title: Sequencing CYP2D6 for the detection of poor-metabolizers in post-mortem blood samples with tramadol.
Article Snippet: After purification with ExoSAP-IT1 (Affymetrix) the amplification was confirmed by SDS-PAGE Electrophoresis with Silver Staining in a Phastsystem (GE Healthcare). .. The modified Sanger sequencing reaction was performed with BigDye Terminator v.3.1 Cycle Sequencing Kit of AB, using 4 mL of Better Buffer (Microzone); DMSO; 500 nM of each of the PCR primers and 1 mL of amplified template, toa final volume of 10 mL. ..

Control:

Article Title: Genetic portrait of Lisboa immigrant population from Cabo Verde with mitochondrial DNA analysis.
Article Snippet: The amplified products were purified with ExoSAPIT (USB Corporation, Ohio, USA) method. .. The light and heavy chains from each of the two fragments amplified from the mtDNA control region were sequenced in a final volume of 8 μL containing Better Buffer (Microzone, Sussex, UK), Big Dye Terminator ver. .. 3.1 Cycle Sequence (ABI, Foster City, USA) and primers at 2.5 μM, in a GeneAmp PCR system 9700 (ABI, Foster City, USA) thermo cycler.

Sequencing:

Article Title: Profilin1 E117G is a moderate risk factor for amyotrophic lateral sclerosis
Article Snippet: The PCR product was purified from the amplification mixture using microCLEAN PCR clean-up kit (Microzone), and the repeat region of interest was sequenced using ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit V.1.1. .. 15 μL sequencing reactions comprised of 5–20 ng of DNA template, 1 μL BigDye mix, 5 μL Better Buffer (Microzone), 5 pmol of the forward and reverse sequencing primer (same as PCR primers), 1 μL betaine and were made up with an appropriate volume of nuclease-free water. ..

Article Title: Diagnosis of Spinocerebellar Ataxias Caused by Trinucleotide Repeat Expansions.
Article Snippet: Spinocerebellar ataxias (SCAs) are a group of disorders that are both clinically and genetically heterogeneous.. They usually demonstrate onset in adulthood, but some forms may have juvenile or infantile onset.. There are many different types of SCA, demonstrating different modes of inheritance and types of mutation.

Article Title: Sequencing CYP2D6 for the detection of poor-metabolizers in post-mortem blood samples with tramadol.
Article Snippet: After purification with ExoSAP-IT1 (Affymetrix) the amplification was confirmed by SDS-PAGE Electrophoresis with Silver Staining in a Phastsystem (GE Healthcare). .. The modified Sanger sequencing reaction was performed with BigDye Terminator v.3.1 Cycle Sequencing Kit of AB, using 4 mL of Better Buffer (Microzone); DMSO; 500 nM of each of the PCR primers and 1 mL of amplified template, toa final volume of 10 mL. ..

Article Title: Novel class II alpha MHC variability in a small peripheral Atlantic salmon population.
Article Snippet: Background: The highly polymorphic genes of the major histocompatibility complex (MHC) are some of the most studied immune related genes in vertebrates.. MHC genes encode for proteins involved in the adaptive immune response by presenting pathogen-derived antigens to Tcells.. MHC diversity can be maintained by balancing selection driven by pathogens and/or by mate choice.

Article Title: Diversity of aminopeptidases, derived from four lepidopteran gene duplications, and polycalins expressed in the midgut of Helicoverpa armigera : Identification of proteins binding the δ -endotoxin, Cry1Ac of Bacillus thuringiensis
Article Snippet: Clones containing aminopeptidase sequences were further sequenced with a CEQTM 2000 (Dye Terminator Cycle Sequencing with Quick Start Kit) from Beckman Coulter. .. Sequencing reactions contained, in 10 μl total volume, 100–300 ng plasmid DNA, 2 μl Quick Start Master Mix, 2 μl Better Buffer (Microzone Ltd., West Sussex, UK), and 10 pmol reverse primer. ..

Article Title: PRNP allelic series from 19 years of prion protein gene sequencing at the MRC Prion Unit.
Article Snippet: Amplicons were cleaned using MicroCLEAN (Microzone) then resuspended in 40μl 18MΩ ddH20. .. Sequencing reactions included BigDye v1.1 (Applied Biosystems) and BetterBuffer (Microzone) and both forward and reverse DNA strands were assessed. .. Standard run conditions were applied to electrophoresis of sequenced products on an Applied Biosystems 3130xl, using polymer POP7, 50cm arrays and standard run module with sample injection time of 15 seconds.

Polymerase Chain Reaction:

Article Title: Profilin1 E117G is a moderate risk factor for amyotrophic lateral sclerosis
Article Snippet: The PCR product was purified from the amplification mixture using microCLEAN PCR clean-up kit (Microzone), and the repeat region of interest was sequenced using ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit V.1.1. .. 15 μL sequencing reactions comprised of 5–20 ng of DNA template, 1 μL BigDye mix, 5 μL Better Buffer (Microzone), 5 pmol of the forward and reverse sequencing primer (same as PCR primers), 1 μL betaine and were made up with an appropriate volume of nuclease-free water. ..

Article Title: Diagnosis of Spinocerebellar Ataxias Caused by Trinucleotide Repeat Expansions.
Article Snippet: Spinocerebellar ataxias (SCAs) are a group of disorders that are both clinically and genetically heterogeneous.. They usually demonstrate onset in adulthood, but some forms may have juvenile or infantile onset.. There are many different types of SCA, demonstrating different modes of inheritance and types of mutation.

Article Title: Sequencing CYP2D6 for the detection of poor-metabolizers in post-mortem blood samples with tramadol.
Article Snippet: After purification with ExoSAP-IT1 (Affymetrix) the amplification was confirmed by SDS-PAGE Electrophoresis with Silver Staining in a Phastsystem (GE Healthcare). .. The modified Sanger sequencing reaction was performed with BigDye Terminator v.3.1 Cycle Sequencing Kit of AB, using 4 mL of Better Buffer (Microzone); DMSO; 500 nM of each of the PCR primers and 1 mL of amplified template, toa final volume of 10 mL. ..

Article Title: Novel class II alpha MHC variability in a small peripheral Atlantic salmon population.
Article Snippet: Background: The highly polymorphic genes of the major histocompatibility complex (MHC) are some of the most studied immune related genes in vertebrates.. MHC genes encode for proteins involved in the adaptive immune response by presenting pathogen-derived antigens to Tcells.. MHC diversity can be maintained by balancing selection driven by pathogens and/or by mate choice.

Article Title: p63 is an alternative p53 repressor in melanoma that confers chemoresistance and a poor prognosis
Article Snippet: PCR products were sequenced using the BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems). .. 0.3 μl PCR product was added to a reaction containing 1 μl BigDye terminator mix (version 3.1; Applied Biosystems), 1 μl of 10 μM primer, and 3 μl Better Buffer (Microzone) made up to 11.5 μl with dH 2 O. ..

High Performance Liquid Chromatography:

Article Title: Diagnosis of Spinocerebellar Ataxias Caused by Trinucleotide Repeat Expansions.
Article Snippet: Spinocerebellar ataxias (SCAs) are a group of disorders that are both clinically and genetically heterogeneous.. They usually demonstrate onset in adulthood, but some forms may have juvenile or infantile onset.. There are many different types of SCA, demonstrating different modes of inheritance and types of mutation.

Purification:

Article Title: Diagnosis of Spinocerebellar Ataxias Caused by Trinucleotide Repeat Expansions.
Article Snippet: Spinocerebellar ataxias (SCAs) are a group of disorders that are both clinically and genetically heterogeneous.. They usually demonstrate onset in adulthood, but some forms may have juvenile or infantile onset.. There are many different types of SCA, demonstrating different modes of inheritance and types of mutation.

Article Title: Novel class II alpha MHC variability in a small peripheral Atlantic salmon population.
Article Snippet: Background: The highly polymorphic genes of the major histocompatibility complex (MHC) are some of the most studied immune related genes in vertebrates.. MHC genes encode for proteins involved in the adaptive immune response by presenting pathogen-derived antigens to Tcells.. MHC diversity can be maintained by balancing selection driven by pathogens and/or by mate choice.

Polyacrylamide Gel Electrophoresis:

Article Title: Diagnosis of Spinocerebellar Ataxias Caused by Trinucleotide Repeat Expansions.
Article Snippet: Spinocerebellar ataxias (SCAs) are a group of disorders that are both clinically and genetically heterogeneous.. They usually demonstrate onset in adulthood, but some forms may have juvenile or infantile onset.. There are many different types of SCA, demonstrating different modes of inheritance and types of mutation.

Modification:

Article Title: Sequencing CYP2D6 for the detection of poor-metabolizers in post-mortem blood samples with tramadol.
Article Snippet: After purification with ExoSAP-IT1 (Affymetrix) the amplification was confirmed by SDS-PAGE Electrophoresis with Silver Staining in a Phastsystem (GE Healthcare). .. The modified Sanger sequencing reaction was performed with BigDye Terminator v.3.1 Cycle Sequencing Kit of AB, using 4 mL of Better Buffer (Microzone); DMSO; 500 nM of each of the PCR primers and 1 mL of amplified template, toa final volume of 10 mL. ..

Plasmid Preparation:

Article Title: Diversity of aminopeptidases, derived from four lepidopteran gene duplications, and polycalins expressed in the midgut of Helicoverpa armigera : Identification of proteins binding the δ -endotoxin, Cry1Ac of Bacillus thuringiensis
Article Snippet: Clones containing aminopeptidase sequences were further sequenced with a CEQTM 2000 (Dye Terminator Cycle Sequencing with Quick Start Kit) from Beckman Coulter. .. Sequencing reactions contained, in 10 μl total volume, 100–300 ng plasmid DNA, 2 μl Quick Start Master Mix, 2 μl Better Buffer (Microzone Ltd., West Sussex, UK), and 10 pmol reverse primer. ..



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