barcoding strategies (Oxford Nanopore)
90
Structured Review
Oxford Nanopore
barcoding strategies
Barcoding Strategies, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/barcoding+strategy/barcodes/pmc08244870-148-9-6
Average 90 stars, based on 1 article reviews
Barcoding Strategies, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/barcoding+strategy/barcodes/pmc08244870-148-9-6
Average 90 stars, based on 1 article reviews
barcoding strategies - by Bioz Stars,
2026-09
90/100 stars
Images
1) Product Images from "A CRISPR-based assay for the study of eukaryotic DNA repair onboard the International Space Station"
Article Title: A CRISPR-based assay for the study of eukaryotic DNA repair onboard the International Space Station
Journal: PLoS ONE
doi: 10.1371/journal.pone.0253403
Figure Legend Snippet: A. Map of the pVG1 vector . This vector contains CRISPR machinery: Cas9, guide RNAs targeting ADE2 , a repair template that introduces two stop codons and an EcoRI site into the ADE2 gene, and the URA3 gene for positive selection. B. ADE2 mutant colonies are easily distinguished from those bearing the wild type ADE2 sequence. ADE2 is not essential for survival, but S . cerevisiae with mutations in this gene turn red due to the buildup of purine precursors in the vacuole . Wild type S . cerevisiae colonies are white. C. Adaptation of S . cerevisiae transformation and CRISPR/Cas9 genome editing protocols for use onboard the ISS. Prior to launch, cells were grown in liquid culture on Earth, pelleted by centrifugation, and frozen in glycerol at -80°C for transport to the ISS. Step 1, transformation: transformation mixture and pVG1 vector were added to thawed cells. The miniPCR thermal cycler was used as a heat block to induce transformation. Following transformation, cells were plated on synthetic defined agar lacking uracil (SDA-URA) and grown at room temperature for six days when the phenotype of the colonies was assessed. Step 2, DNA extraction: A pipette tip was used to transfer a small number of cells from four red and four white colonies to the DNA extraction buffer. Cells were heated in the miniPCR thermal cycler to 95°C to extract the DNA. Step 3, PCR and barcoding: DNA extract was directly added to PCR reagents. PCR was performed to amplify the 5’ end of the ADE2 gene. Sequencing barcodes were added at this step. Step 4, sample pooling and magnetic bead clean up: PCR product was pooled and purified during a magnetic bead cleanup step. Step 5, nanopore sequencing: Purified PCR product was sequenced by nanopore sequencing. Data was downlinked to the ground where sequences were assessed.
Techniques Used: Plasmid Preparation, CRISPR, Selection, Mutagenesis, Sequencing, Transformation Assay, Centrifugation, Blocking Assay, DNA Extraction, Transferring, Purification, Nanopore Sequencing
Related Articles
Extraction:Article Title: Uncovering the magnitude of African pangolin poaching with extensive nanopore DNA genotyping of seized scales. Article Snippet: .. The key innovations in our approach lie in the use of Oxford Nanopore Technologies ( other:Article Title: Discovery and characterization of a novel telomerase alternative splicing isoform that protects lung cancer cells from chemotherapy induced cell death. Article Snippet: After size exclusion of the TERT speci c library, Article Title: Draft genome of Brasenia schreberi, a worldwide distributed and endangered aquatic plant Article Snippet: : Pipeline for Article Title: A Longitudinal Study of Dominant E. coli Lineages and Antimicrobial Resistance in the Gut of Children Living in an Upper Middle-income Country Article Snippet: Article Title: Discovery and characterization of a novel telomerase alternative splicing isoform that protects lung cancer cells from chemotherapy induced cell death. Article Snippet: After size exclusion of the TERT specific library, Amplification:Article Title: Evolution of isoform-level gene expression patterns across tissues during lotus species divergence. Article Snippet: A SuperScript IV First-Strand Synthesis System (Invitrogen, USA) was used for full-length mRNA reverse transcription. .. According to the Oxford Nanopore recommended protocol, Ligation:Article Title: Genetic evidence that uptake of the fluorescent analog 2NBDG occurs independently of known glucose transporters Article Snippet: .. Equimolar concentrations of amplicons from different reactions were then pooled and 300fmol of the sample was end-prep treated and ligated with Sequencing:Article Title: Genetic evidence that uptake of the fluorescent analog 2NBDG occurs independently of known glucose transporters Article Snippet: .. Equimolar concentrations of amplicons from different reactions were then pooled and 300fmol of the sample was end-prep treated and ligated with |