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access array barcode library  (fluidigm)


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    Structured Review

    fluidigm access array barcode library
    Access Array Barcode Library, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 843 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/barcoded+libraries/Access+Array/pm41722379-80-20-19
    Average 93 stars, based on 843 article reviews
    access array barcode library - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Paralleled Dynamics of Arabidopsis Root Exudation and SynCom Assembly in a Controlled Environment
    Article Snippet: PCR1 amplicons were purified using the ChargeSwitch® PCR Clean-Up Kit (Invitrogen, Waltham, MA, USA) on a KingFisherTM FlexTM Purification System (ThermoFisher Scientific, Waltham, MA, USA). .. Unique Access Array barcoding primers (Standard BioTools, CA, USA) were added in a second PCR (PCR2) using 5 μL of purified PCR1 product following previously reported cycling conditions (Rensburg et al., 2025). ..

    Article Title: Argan fruit microbiomes: influence of biogeographic and soil driven environmental factors
    Article Snippet: A second PCR was used to attach Illumina sequencing adapters and index tags. .. This reaction included 5 μL of purified PCR product, 2.5 μL of Fluidigm Access Array Barcode 384, and 1X KAPA HiFi HotStart ReadyMix (Roche Sequencing Solutions), in total volume of 50 μl. ..

    Purification:

    Article Title: Paralleled Dynamics of Arabidopsis Root Exudation and SynCom Assembly in a Controlled Environment
    Article Snippet: PCR1 amplicons were purified using the ChargeSwitch® PCR Clean-Up Kit (Invitrogen, Waltham, MA, USA) on a KingFisherTM FlexTM Purification System (ThermoFisher Scientific, Waltham, MA, USA). .. Unique Access Array barcoding primers (Standard BioTools, CA, USA) were added in a second PCR (PCR2) using 5 μL of purified PCR1 product following previously reported cycling conditions (Rensburg et al., 2025). ..

    Article Title: Argan fruit microbiomes: influence of biogeographic and soil driven environmental factors
    Article Snippet: A second PCR was used to attach Illumina sequencing adapters and index tags. .. This reaction included 5 μL of purified PCR product, 2.5 μL of Fluidigm Access Array Barcode 384, and 1X KAPA HiFi HotStart ReadyMix (Roche Sequencing Solutions), in total volume of 50 μl. ..

    High Throughput Screening Assay:

    Article Title: High-frequency oscillatory ventilation during physiological-based cord clamping attenuates inflammation in preterm lambs.
    Article Snippet: 10.1136/arch d isch ild -2025-329610 o n is C h ild F etal N eo n atal E d : first p u b lish ed as F3Benincasa BC, et al. Arch Dis Child Fetal Neonatal Ed 2026;0:F1–F8. doi:10.1136/archdischild- 2025- 329610 Original research and CD163- positive cells were manually quantified using Fiji/ ImageJ (V.2.14.0, LOCI, Wisconsin, USA) and expressed as total and relative to total cell counts. .. High- throughput real- time quantitative PCR using Fluidigm Access Array System Technology (Fluidigm, California, USA) and TaqMan primers (Thermo Fisher Scientific, Massachusetts, USA) was undertaken to quantify messenger RNA (mRNA) expression of 20 curated genes (online supplemental table 1). ..

    Real-time Polymerase Chain Reaction:

    Article Title: High-frequency oscillatory ventilation during physiological-based cord clamping attenuates inflammation in preterm lambs.
    Article Snippet: 10.1136/arch d isch ild -2025-329610 o n is C h ild F etal N eo n atal E d : first p u b lish ed as F3Benincasa BC, et al. Arch Dis Child Fetal Neonatal Ed 2026;0:F1–F8. doi:10.1136/archdischild- 2025- 329610 Original research and CD163- positive cells were manually quantified using Fiji/ ImageJ (V.2.14.0, LOCI, Wisconsin, USA) and expressed as total and relative to total cell counts. .. High- throughput real- time quantitative PCR using Fluidigm Access Array System Technology (Fluidigm, California, USA) and TaqMan primers (Thermo Fisher Scientific, Massachusetts, USA) was undertaken to quantify messenger RNA (mRNA) expression of 20 curated genes (online supplemental table 1). ..

    Expressing:

    Article Title: High-frequency oscillatory ventilation during physiological-based cord clamping attenuates inflammation in preterm lambs.
    Article Snippet: 10.1136/arch d isch ild -2025-329610 o n is C h ild F etal N eo n atal E d : first p u b lish ed as F3Benincasa BC, et al. Arch Dis Child Fetal Neonatal Ed 2026;0:F1–F8. doi:10.1136/archdischild- 2025- 329610 Original research and CD163- positive cells were manually quantified using Fiji/ ImageJ (V.2.14.0, LOCI, Wisconsin, USA) and expressed as total and relative to total cell counts. .. High- throughput real- time quantitative PCR using Fluidigm Access Array System Technology (Fluidigm, California, USA) and TaqMan primers (Thermo Fisher Scientific, Massachusetts, USA) was undertaken to quantify messenger RNA (mRNA) expression of 20 curated genes (online supplemental table 1). ..

    Amplification:

    Article Title: Fungal community structure and network connectivity as indicators of soil health under long-term land use.
    Article Snippet: Sequencing (Miseq) was performed at the Hylabs Laboratory Ltd. (Rehovot, Israel; www.hylabs.co.il) sequencing facility using an Illumina sequencing platform (Illumina Inc., San Diego, CA, USA). .. Protocol PCR2: 2ul sample from PCR1 amplified sample containing CS1/CS2 adaptors was amplified for 10 cycles in 10ul using Fluidigm Access Array Barcode library according to manufacturer’s protocol (2ul barcode per reaction). .. DNA was purified using Kapa Pure Beads at a ratio of 0.65X and quantified with qubit using Denovix DsDNA high sensitivity assay.

    Sequencing:

    Article Title: Genetic variations in AAK1 and ADAM17 associated with circulatory cytokines changes influence COVID-19 susceptibility and severity
    Article Snippet: .. The samples were prepared using the Fluidigm 48.48 Access Array integrated fluidic circuit (IFC) (Fluidigm Europe B.V., Netherlands) for sequencing using the Ion 520TM Chip on an Ion S5 XL Semiconductor (ThermoFisher, USA) as previously described [ ]. ..

    Article Title: Genetic variations in AAK1 and ADAM17 associated with circulatory cytokines changes influence COVID-19 susceptibility and severity.
    Article Snippet: .. The samples were prepared using the Fluidigm 48.48 Access Array integrated fluidic circuit (IFC) (Fluidigm Europe B.V., Netherlands) for sequencing using the Ion 520TM Chip on an Ion S5 XL Semiconductor (ThermoFisher, USA) as previously described (30). ..

    Mutagenesis:

    Article Title: Familial medullary thyroid carcinoma secondary to an SLC30A9 intragenic deletion and translation reinitiation
    Article Snippet: .. The probands from the RET mutation-negative MTC families and all patients with RET mutation-negative sporadic MTC were sequenced for SLC30A9 (NM_006345.4) using the Fluidigm Access Array (Fluidigm, South San Francisco, CA, USA). ..

    Immunopeptidomics:

    Article Title: Dynamics of natural and pharmacologic control of an SIV variant with an envelope trafficking defect
    Article Snippet: .. Briefly, a panel of oligonucleotides flanking the highly polymorphic peptide domains encoded by exon 2 of MHC class I and class II loci were used for multiplex PCRs with an Access Array 48.48 (Standard BioTools) following the manufacturer’s protocol. .. This amplicon library was sequenced on an Illumina MiSeq instrument, and the resulting sequence reads were mapped against a custom reference database of Mane MHC sequences ( https://github.com/dholab/mhc_genotyper ).

    Multiplex Assay:

    Article Title: Dynamics of natural and pharmacologic control of an SIV variant with an envelope trafficking defect
    Article Snippet: .. Briefly, a panel of oligonucleotides flanking the highly polymorphic peptide domains encoded by exon 2 of MHC class I and class II loci were used for multiplex PCRs with an Access Array 48.48 (Standard BioTools) following the manufacturer’s protocol. .. This amplicon library was sequenced on an Illumina MiSeq instrument, and the resulting sequence reads were mapped against a custom reference database of Mane MHC sequences ( https://github.com/dholab/mhc_genotyper ).



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    Image Search Results


    a) Schematic overview of the experimental design. Mouse PDAC cell lines were clonally barcoded using a lentiviral library (1), expanded and orthotopically transplanted into syngeneic immunocompetent mice (2–3), followed by scRNA-seq profiling of resultant tumours (4). Expressed barcode tracing enabled unambiguous separation of malignant (TAG⁺) from host-derived non-malignant cells (right panel, UMAPs depicting barcoded (TAG + ) (top) and malignant (bottom) cells). b-e) UMAPs of the integrated Mouse PDAC Atlas coloured by dataset (b), sex (c), treatment type (d), and model (orthotopic syngeneic immunocompetent allografts vs. autochthonous GEMMs (e). f) Sample-wise cell-type composition across treatment types, datasets, and models. Autochthonous tumours were dominated by classical epithelial-like malignant states, whereas orthotopic allografts displayed greater heterogeneity with an enrichment of EMT, hypoxic, and mesenchymal programs. g ) Level 3 hierarchical annotation of the Mouse Atlas using the same multi-tiered scheme as the Human Atlas, resolving lymphoid, myeloid, stromal, endocrine, exocrine, endothelial, and malignant compartments. h-j) Substate resolution of major immune and stromal lineages: CD4⁺ T cells (h), CD8⁺ T cells (i), and macrophages (j), showing distinct regulatory, effector, angiogenic, and lipid-processing programs. k) Validation of double-positive (DP) CD4⁺CD8⁺ T cells at the transcriptomics level (transcription density plots, left panel) and at the protein level by flow cytometry (right panel). l) UMAP showing DP T cells coloured by species (left) and Pearson correlation of mouse DP T cell gene expression against the human DP T cell archetype (right).

    Journal: bioRxiv

    Article Title: Cross-species single-cell atlases chart progression, therapy-driven remodelling and immune evasion in pancreatic cancer

    doi: 10.64898/2026.03.19.712924

    Figure Lengend Snippet: a) Schematic overview of the experimental design. Mouse PDAC cell lines were clonally barcoded using a lentiviral library (1), expanded and orthotopically transplanted into syngeneic immunocompetent mice (2–3), followed by scRNA-seq profiling of resultant tumours (4). Expressed barcode tracing enabled unambiguous separation of malignant (TAG⁺) from host-derived non-malignant cells (right panel, UMAPs depicting barcoded (TAG + ) (top) and malignant (bottom) cells). b-e) UMAPs of the integrated Mouse PDAC Atlas coloured by dataset (b), sex (c), treatment type (d), and model (orthotopic syngeneic immunocompetent allografts vs. autochthonous GEMMs (e). f) Sample-wise cell-type composition across treatment types, datasets, and models. Autochthonous tumours were dominated by classical epithelial-like malignant states, whereas orthotopic allografts displayed greater heterogeneity with an enrichment of EMT, hypoxic, and mesenchymal programs. g ) Level 3 hierarchical annotation of the Mouse Atlas using the same multi-tiered scheme as the Human Atlas, resolving lymphoid, myeloid, stromal, endocrine, exocrine, endothelial, and malignant compartments. h-j) Substate resolution of major immune and stromal lineages: CD4⁺ T cells (h), CD8⁺ T cells (i), and macrophages (j), showing distinct regulatory, effector, angiogenic, and lipid-processing programs. k) Validation of double-positive (DP) CD4⁺CD8⁺ T cells at the transcriptomics level (transcription density plots, left panel) and at the protein level by flow cytometry (right panel). l) UMAP showing DP T cells coloured by species (left) and Pearson correlation of mouse DP T cell gene expression against the human DP T cell archetype (right).

    Article Snippet: For clonal and state-fate analysis by single-cell RNA-seq, primary mouse PDAC cells were clonally tagged with expressed DNA barcodes using the LARRY Barcode Library (Addgene #140024; RRID: Addgene 140024) .

    Techniques: Derivative Assay, Biomarker Discovery, Transcriptomics, Flow Cytometry, Gene Expression

    a Schematic representation of experimental workflow for single-nucleus short-read RNA-sequencing and Kinnex long-read RNA-sequencing. Figure 1a. was partially created with BioRender. https://BioRender.com/c44n540 . b UMAP plot colored by cell type assignments. c Bar plot showing the proportions of each cell type in AD and ND samples (ns: not significant; Two-sided t-test). d Differentially expressed genes (DEGs) identified across different cell types in AD and ND samples (absolute log2 fold change > 0.25 and adjusted p -value < 0.05, Wilcoxon Rank Sum test with Bonferroni correction). e–h Circos plots of five selected Gene Ontology (GO) terms for excitatory neurons, microglia, oligodendrocytes, and astrocytes with associated genes (false discovery rate (FDR) of <0.05 with the Benjamini-Hochberg (BH) test). Conserved GO terms are shown in the same color.

    Journal: Communications Biology

    Article Title: RNA isoform diversity, splicing variants and switching in single cells of the Alzheimer’s disease brain

    doi: 10.1038/s42003-026-09759-9

    Figure Lengend Snippet: a Schematic representation of experimental workflow for single-nucleus short-read RNA-sequencing and Kinnex long-read RNA-sequencing. Figure 1a. was partially created with BioRender. https://BioRender.com/c44n540 . b UMAP plot colored by cell type assignments. c Bar plot showing the proportions of each cell type in AD and ND samples (ns: not significant; Two-sided t-test). d Differentially expressed genes (DEGs) identified across different cell types in AD and ND samples (absolute log2 fold change > 0.25 and adjusted p -value < 0.05, Wilcoxon Rank Sum test with Bonferroni correction). e–h Circos plots of five selected Gene Ontology (GO) terms for excitatory neurons, microglia, oligodendrocytes, and astrocytes with associated genes (false discovery rate (FDR) of <0.05 with the Benjamini-Hochberg (BH) test). Conserved GO terms are shown in the same color.

    Article Snippet: Single-nucleus barcoded cDNA libraries were generated using the 10X Genomics Single Cell 3’ v3.1 kit.

    Techniques: RNA Sequencing