axiovision colocalization module (Carl Zeiss)
Structured Review

Axiovision Colocalization Module, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/axiovision+colocalization+module/coefficient+colocalization+function+software/pmc05637056-69-11-14
Average 90 stars, based on 1 article reviews
Images
1) Product Images from "The α-melanocyte stimulating hormone/peroxisome proliferator activated receptor-γ pathway down-regulates proliferation in melanoma cell lines"
Article Title: The α-melanocyte stimulating hormone/peroxisome proliferator activated receptor-γ pathway down-regulates proliferation in melanoma cell lines
Journal: Journal of Experimental & Clinical Cancer Research : CR
doi: 10.1186/s13046-017-0611-4
Figure Legend Snippet: Analysis of PPARγ translocation into the nucleus and activity in response to 3 M3 exposure. ( a , b , c ) Immunofluorescence analysis of PPARγ localization in untreated cells ( A - C , G - I ) and in cells treated with 15 μM 3 M3 for 3 h ( D - F , J - L ). Immunolabeling with anti-PPARγ antibody and nuclear staining with DAPI. Scale bar: 20 μM. ( c ) Quantitative analysis of the PPARγ/DAPI colocalization signal in the nucleus. Results are express as fold increase of colocalization signal with respect to the values obtained in untreated cells and are reported as mean value ± SD (%) (* p < 0.01). ( d ) Transcriptional activity of PPARγ (fold change) by luciferase activity assay in B16-F10 and Mel 13. Cells were transfected with pGL3-(Jwt)3TKLuc reporter construct. After 24 h of transfection, cells were treated with 15 μM 3 M3. The measurement of luciferase activity was carried out 24 h after treatment. The variability of transfection was normalized with β -Gal activity. The results were expressed as fold change with respect to untreated cells. Data are mean values ± SD of three independent experiments performed in triplicate. * p < 0.01 (vs untreated cells)
Techniques Used: Translocation Assay, Activity Assay, Immunofluorescence, Immunolabeling, Staining, Luciferase, Transfection, Construct
Related Articles
Control:Article Title: Keratin-72 restricts HIV-1 infection in resting CD4 + T cells by sequestering capsids in intermediate filaments. Article Snippet: HeLa cells, MDMs, or MDDCs were fixed and stained primarily with anti-p24 that was probedwith an Alexa Fluor 488 conjugated goat anti-rabbit or anti-mouse secondary antibody (Imag-iT Fixation/Permeabilization Kit, Invitrogen) according to the manufacturer’s instructions. .. The colocalization coefficient of mCherry-KRT72 or control signals with HIV-1 core signals from INmNG (VSV-G-pseudotyped HIV-1) or anti-p24 antibody (WT HIV-1) staining was calculated by the Staining:Article Title: Keratin-72 restricts HIV-1 infection in resting CD4 + T cells by sequestering capsids in intermediate filaments. Article Snippet: HeLa cells, MDMs, or MDDCs were fixed and stained primarily with anti-p24 that was probedwith an Alexa Fluor 488 conjugated goat anti-rabbit or anti-mouse secondary antibody (Imag-iT Fixation/Permeabilization Kit, Invitrogen) according to the manufacturer’s instructions. .. The colocalization coefficient of mCherry-KRT72 or control signals with HIV-1 core signals from INmNG (VSV-G-pseudotyped HIV-1) or anti-p24 antibody (WT HIV-1) staining was calculated by the Software:Article Title: Keratin-72 restricts HIV-1 infection in resting CD4 + T cells by sequestering capsids in intermediate filaments. Article Snippet: HeLa cells, MDMs, or MDDCs were fixed and stained primarily with anti-p24 that was probedwith an Alexa Fluor 488 conjugated goat anti-rabbit or anti-mouse secondary antibody (Imag-iT Fixation/Permeabilization Kit, Invitrogen) according to the manufacturer’s instructions. .. The colocalization coefficient of mCherry-KRT72 or control signals with HIV-1 core signals from INmNG (VSV-G-pseudotyped HIV-1) or anti-p24 antibody (WT HIV-1) staining was calculated by the Article Title: Establishment and validation of a plasma oncofetal chondroitin sulfated proteoglycan for pan-cancer detection Article Snippet: The coverslips were washed and then mounted to the cover slide with an antifade mounting medium (Absin, Cat# abs9234) and left to cure for 24 h. Images were taken on a Zeiss LSM 880 confocal microscope at × 40 magnification. .. Article Title: Novel protein complexes containing autophagy and UPS components regulate proteasome-dependent PARK2 recruitment onto mitochondria and PARK2-PARK6 activity during mitophagy Article Snippet: .. Colocalization coefficient of mitochondria with LC3, PSMA7, ATG5-12, PARK6 or PARK2 was analyzed using Article Title: APOE- ε4-induced Fibronectin at the blood-brain barrier is a conserved pathological mediator of disrupted astrocyte-endothelia interaction in Alzheimer’s disease Article Snippet: .. For fluorescence intensity colocalization, Manders colocalization coefficient ( ) was calculated using Article Title: Tetraspanin-enriched microdomains play an important role in pathogenesis in the protozoan parasite Entamoeba histolytica. Article Snippet: .. Scatter plots of immunofluorescence images from Fig 3A were generated using the other:Article Title: Tumor-derived erythropoietin acts as an immunosuppressive switch in cancer immunity Article Snippet: Plasma EPO levels were determined using the Mouse Erythropoietin/EPO Quantikine ELISA Kit (R&D Systems). Article Title: S-palmitoylation determines TMEM55B-dependent positioning of lysosomes. Article Snippet: Image acquisition and processing were performed with the Fluorescence:Article Title: APOE- ε4-induced Fibronectin at the blood-brain barrier is a conserved pathological mediator of disrupted astrocyte-endothelia interaction in Alzheimer’s disease Article Snippet: .. For fluorescence intensity colocalization, Manders colocalization coefficient ( ) was calculated using Immunofluorescence:Article Title: Tetraspanin-enriched microdomains play an important role in pathogenesis in the protozoan parasite Entamoeba histolytica. Article Snippet: .. Scatter plots of immunofluorescence images from Fig 3A were generated using the Generated:Article Title: Tetraspanin-enriched microdomains play an important role in pathogenesis in the protozoan parasite Entamoeba histolytica. Article Snippet: .. Scatter plots of immunofluorescence images from Fig 3A were generated using the |