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axio observer7 inverted fluorescence microscope  (Carl Zeiss)


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    Structured Review

    Carl Zeiss axio observer7 inverted fluorescence microscope
    Axio Observer7 Inverted Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 96/100, based on 4909 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axio+observer7+microscope/Inverted+microscope+Axio+Observer+7+materials/pm40545106-117-7-12
    Average 96 stars, based on 4909 article reviews
    axio observer7 inverted fluorescence microscope - by Bioz Stars, 2026-11
    96/100 stars

    Images

    Related Articles

    Inverted Microscopy:

    Article Title: Depletion of S100A4+ stromal cells results in abnormal nipple development and nursing failure
    Article Snippet: .. Whole-mounts were photographed using an inverted microscope Zeiss Axio Observer.7 with LSM 880 laser scanning unit using 405 nm, 488 nm and 561 nm lasers, a GaAsP detector, and Plan-Apochromat objectives 10x/0.45 AIR, Plan-Apochromat 25x / 0.80 MIM with oil immersion (all Zeiss). ..

    Article Title: Cadherin-11 integrates Piezo1 and interleukin-6 signaling to promote fibroblast activation
    Article Snippet: The next day, hydrogels were washed 3 times with PBS then incubated with rhodamine phalloidin (1:400, ThermoFisher R415) and secondary antibodies (AlexaFluor 488 goat anti-rabbit IgG, 1:400; AlexaFluor 647 goat anti-mouse, 1:400) for 2 h at room temperature protected from light. .. Hydrogels were again rinsed with PBS 3 times then stained with 4′,6-diamidino-2-phenylindole (DAPI, 1:5000, ThermoFisher D1306) for 5 min. After rinsing 3 times with PBS, hydrogels were stored protected from light at 4°C until imaging on a Zeiss AxioObserver 7 inverted microscope. .. Cell area, cell shape index (form factor), and staining intensity of CDH11 and Piezo1 were evaluated using a CellProfiler (Broad Institute, Harvard/MIT) pipeline.

    Fluorescence:

    Article Title: On-demand cancer immunotherapy via single-cell encapsulation of synthetic circuit–engineered cells
    Article Snippet: .. Fluorescent images were acquired using an inverted fluorescence microscope (Axio Observer 7, Zeiss, Germany) with standard fluorescein isothiocyanate [excitation/emission (ex/em), 495/515 nm] and tetramethyl rhodamine isothiocyanate (ex/em, 535/617 nm) filter sets for calcein-AM (green fluorescence, viable cells) and PI (red fluorescence, nonviable cells), respectively. .. Fluorescent images were acquired using an inverted fluorescence microscope (Axio Observer 7, Zeiss, Germany) with standard fluorescein isothiocyanate [excitation/emission (ex/em), 495/515 nm] and tetramethyl rhodamine isothiocyanate (ex/em, 535/617 nm) filter sets for calcein-AM (green fluorescence, viable cells) and PI (red fluorescence, nonviable cells), respectively.

    Article Title: Biosurfactant polysaccharide drives dispersal of exopolysaccharide-coated myxospores in Myxococcus xanthus
    Article Snippet: .. For detection of red-fluorescent mCherry signal, slides were imaged at 40× magnification with an alpha Plan-Apochromat oil-immersion objective (captured with 50% LED illumination intensity, 150-ms exposure time, binning mode: 2 × 2, reflector: 45 Texas Red) on a Zeiss AxioObserver 7 inverted fluorescence microscope with an Axiocam 512 camera. ..

    Article Title: Effort experience as a driver of behavioral modulation in Caenorhabditis elegans
    Article Snippet: .. The chip was mounted on a Zeiss AxioObserver 7 inverted fluorescence microscope with a 40× air objective and a Photometrics Prime 95B sCMOS camera. ..

    Article Title: Chromosome-scale assembly of yeast genomes using enzyme-free, dense optical genome mapping.
    Article Snippet: .. Stretched and stained DNA molecules in the nanochannels were then imaged by using an inverted fluorescence microscope (Zeiss Axio Observer D.1) equipped with a 63x oil immersion objective (NA = 1.46, Zeiss), a Colibri 7 light source (Zeiss), and a Photometrics Evolve EMCCD camera. .. Systematic acquisition of videos of up to 100 frames was carriedout for eachmolecule, utilizing anexposure timeof 100msand a FITC filter set (Zeiss).

    Article Title: A single valine to leucine switch disrupts Plasmodium falciparum AP2-G DNA binding and reveals GDV1’s role in ap2-g activation
    Article Snippet: .. Live imaging was performed using Zeiss inverted fluorescence microscope (Axio Observer 7) under 630x magnification and ZEN v3.0 (blue edition) in-built analysis software. ..

    Microscopy:

    Article Title: On-demand cancer immunotherapy via single-cell encapsulation of synthetic circuit–engineered cells
    Article Snippet: .. Fluorescent images were acquired using an inverted fluorescence microscope (Axio Observer 7, Zeiss, Germany) with standard fluorescein isothiocyanate [excitation/emission (ex/em), 495/515 nm] and tetramethyl rhodamine isothiocyanate (ex/em, 535/617 nm) filter sets for calcein-AM (green fluorescence, viable cells) and PI (red fluorescence, nonviable cells), respectively. .. Fluorescent images were acquired using an inverted fluorescence microscope (Axio Observer 7, Zeiss, Germany) with standard fluorescein isothiocyanate [excitation/emission (ex/em), 495/515 nm] and tetramethyl rhodamine isothiocyanate (ex/em, 535/617 nm) filter sets for calcein-AM (green fluorescence, viable cells) and PI (red fluorescence, nonviable cells), respectively.

    Article Title: Biosurfactant polysaccharide drives dispersal of exopolysaccharide-coated myxospores in Myxococcus xanthus
    Article Snippet: .. For detection of red-fluorescent mCherry signal, slides were imaged at 40× magnification with an alpha Plan-Apochromat oil-immersion objective (captured with 50% LED illumination intensity, 150-ms exposure time, binning mode: 2 × 2, reflector: 45 Texas Red) on a Zeiss AxioObserver 7 inverted fluorescence microscope with an Axiocam 512 camera. ..

    Article Title: Effort experience as a driver of behavioral modulation in Caenorhabditis elegans
    Article Snippet: .. The chip was mounted on a Zeiss AxioObserver 7 inverted fluorescence microscope with a 40× air objective and a Photometrics Prime 95B sCMOS camera. ..

    Article Title: Chromosome-scale assembly of yeast genomes using enzyme-free, dense optical genome mapping.
    Article Snippet: .. Stretched and stained DNA molecules in the nanochannels were then imaged by using an inverted fluorescence microscope (Zeiss Axio Observer D.1) equipped with a 63x oil immersion objective (NA = 1.46, Zeiss), a Colibri 7 light source (Zeiss), and a Photometrics Evolve EMCCD camera. .. Systematic acquisition of videos of up to 100 frames was carriedout for eachmolecule, utilizing anexposure timeof 100msand a FITC filter set (Zeiss).

    Article Title: A single valine to leucine switch disrupts Plasmodium falciparum AP2-G DNA binding and reveals GDV1’s role in ap2-g activation
    Article Snippet: .. Live imaging was performed using Zeiss inverted fluorescence microscope (Axio Observer 7) under 630x magnification and ZEN v3.0 (blue edition) in-built analysis software. ..

    Inverted Epifluorescence:

    Article Title: Stiffness and viscoelasticity of adipose tissue decellularized extracellular matrix hydrogels influence proliferation, growth pattern, migration and invasion of breast cancer cells.
    Article Snippet: .. The cells were grown in the dECM-derived hydrogels for 15 days, with images taken on days 1, 5, 8, 12 and 15 with the AxioObserver 7 inverted epifluorescence microscope (Zeiss). ..

    Staining:

    Article Title: Chromosome-scale assembly of yeast genomes using enzyme-free, dense optical genome mapping.
    Article Snippet: .. Stretched and stained DNA molecules in the nanochannels were then imaged by using an inverted fluorescence microscope (Zeiss Axio Observer D.1) equipped with a 63x oil immersion objective (NA = 1.46, Zeiss), a Colibri 7 light source (Zeiss), and a Photometrics Evolve EMCCD camera. .. Systematic acquisition of videos of up to 100 frames was carriedout for eachmolecule, utilizing anexposure timeof 100msand a FITC filter set (Zeiss).

    Article Title: Cadherin-11 integrates Piezo1 and interleukin-6 signaling to promote fibroblast activation
    Article Snippet: The next day, hydrogels were washed 3 times with PBS then incubated with rhodamine phalloidin (1:400, ThermoFisher R415) and secondary antibodies (AlexaFluor 488 goat anti-rabbit IgG, 1:400; AlexaFluor 647 goat anti-mouse, 1:400) for 2 h at room temperature protected from light. .. Hydrogels were again rinsed with PBS 3 times then stained with 4′,6-diamidino-2-phenylindole (DAPI, 1:5000, ThermoFisher D1306) for 5 min. After rinsing 3 times with PBS, hydrogels were stored protected from light at 4°C until imaging on a Zeiss AxioObserver 7 inverted microscope. .. Cell area, cell shape index (form factor), and staining intensity of CDH11 and Piezo1 were evaluated using a CellProfiler (Broad Institute, Harvard/MIT) pipeline.

    Imaging:

    Article Title: Cadherin-11 integrates Piezo1 and interleukin-6 signaling to promote fibroblast activation
    Article Snippet: The next day, hydrogels were washed 3 times with PBS then incubated with rhodamine phalloidin (1:400, ThermoFisher R415) and secondary antibodies (AlexaFluor 488 goat anti-rabbit IgG, 1:400; AlexaFluor 647 goat anti-mouse, 1:400) for 2 h at room temperature protected from light. .. Hydrogels were again rinsed with PBS 3 times then stained with 4′,6-diamidino-2-phenylindole (DAPI, 1:5000, ThermoFisher D1306) for 5 min. After rinsing 3 times with PBS, hydrogels were stored protected from light at 4°C until imaging on a Zeiss AxioObserver 7 inverted microscope. .. Cell area, cell shape index (form factor), and staining intensity of CDH11 and Piezo1 were evaluated using a CellProfiler (Broad Institute, Harvard/MIT) pipeline.

    Article Title: A single valine to leucine switch disrupts Plasmodium falciparum AP2-G DNA binding and reveals GDV1’s role in ap2-g activation
    Article Snippet: .. Live imaging was performed using Zeiss inverted fluorescence microscope (Axio Observer 7) under 630x magnification and ZEN v3.0 (blue edition) in-built analysis software. ..

    Software:

    Article Title: A single valine to leucine switch disrupts Plasmodium falciparum AP2-G DNA binding and reveals GDV1’s role in ap2-g activation
    Article Snippet: .. Live imaging was performed using Zeiss inverted fluorescence microscope (Axio Observer 7) under 630x magnification and ZEN v3.0 (blue edition) in-built analysis software. ..



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