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zeiss axioobserver 7  (Carl Zeiss)


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    Structured Review

    Carl Zeiss zeiss axioobserver 7
    Zeiss Axioobserver 7, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 98/100, based on 1033 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axio+observer+microscope/Microscope+stand+Axio+Observer+7/pm42867703-100-5-5
    Average 98 stars, based on 1033 article reviews
    zeiss axioobserver 7 - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Proximity Ligation Assay:

    Article Title: Increased Expression of α-Synuclein Impairs Trafficking of the Essential SNARE Protein Synaptobrevin-2.
    Article Snippet: Immunolabelled coverslips were washed in Milli- Q Type 1 ultrapure H2O twice, dried and mounted on microscope slides with Dako Fluorescence Mounting Medium. .. Fixed cultures, trafficking assays and PLA experiment were imaged with a Zeiss Axio Observer 7 epifluorescence microscope. .. A Zeiss Plan- Neofluar 40× oil- immersion objective (NA 1.3) and Zeiss Axiocam 506 mono microscope camera were used to capture 14- bit images with 2 × 2 binning resulting in pixels 0.227 μm in size.

    Microscopy:

    Article Title: Increased Expression of α-Synuclein Impairs Trafficking of the Essential SNARE Protein Synaptobrevin-2.
    Article Snippet: Immunolabelled coverslips were washed in Milli- Q Type 1 ultrapure H2O twice, dried and mounted on microscope slides with Dako Fluorescence Mounting Medium. .. Fixed cultures, trafficking assays and PLA experiment were imaged with a Zeiss Axio Observer 7 epifluorescence microscope. .. A Zeiss Plan- Neofluar 40× oil- immersion objective (NA 1.3) and Zeiss Axiocam 506 mono microscope camera were used to capture 14- bit images with 2 × 2 binning resulting in pixels 0.227 μm in size.

    Article Title: Mast Cells Regulate Epithelial Barrier Integrity and Myeloid Immune Responses in Experimental Colitis.
    Article Snippet: .. sentative FISH images showing bacterial rRNA signal in colonic sections gnal was detected using a Cy5-labeled probe targeting bacterial ribosomal ed with 20 ×/0.8NA objective using a Zeiss Axio Observer 7 microscope. ..

    Article Title: The Effects of Extracellular Vesicles From Cancer Cells in Acidosis or ER Stress to Endothelial Cells: Angiogenesis and Endothelial Activation
    Article Snippet: .. Images were captured on a ZEISS Axio Observer 7 microscope with 20x and 63x magnifications, the latter using the z‐stack mode at 0.24 μm intervals. ..

    Article Title: Distinct Mitochondrial Recovery Dynamics Following Taxol Removal in Triple-Negative Breast Cancer Cells.
    Article Snippet: .. Briefly, live-cell culture dishes were imaged using a wide-field fluorescence microscope (Zeiss Observer 7, White Plains, NY, USA). ..

    Article Title: A novel approach for reliable differentiation of lymphatic endothelial precursor cells in vitro.
    Article Snippet: Afterwards, samples were immunostained with anti-mouse PDPN (127402, BioLegend), anti-mouse LYVE1 (103-PA50AG, Reliatech), and anti-TdTomato (orb182397, Byorbyt) as the primary antibodies; and Alexa Fluor 647-conjugated anti-hamster (A21451, Invitrogen), Alexa Fluor 488-conjugated anti-rabbit (A11034, Molecular Probes) and Alexa Fluor 555-conjugated anti-goat (A21432, Molecular Probes) as secondary antibodies (Table 1 3 S1). .. Immunofluorescence images were taken using Zeiss AxioObserver 7 and LSM900 confocal microscope (Zeiss). .. The expression of PDPN, SCA-1, CD90.2, CD105, CD44, CD31 (PECAM1), CD34, CD45 and CD11 of the isolated and differentiated cells was detected using the APCconjugated anti-mouse PDPN (127410, BioLegend), PE-Cy7-conjugated anti-mouse SCA-1 (558162, BioLegend), anti-mouse CD90.2 (553009, BD Biosciences), antimouse CD105 (14-1051-82, ThermoFisher), anti-mouse CD44 (553134, BD Biosciences), PE-conjugated antimouse CD31 (553373, BD Biosciences), PE-conjugated anti-mouse CD34 (551387, BD Biosciences), anti-mouse CD45 (550539, BD Biosciences) and anti-mouse CD11 (M1/70.15.11.5.2, DSHB).

    Article Title: Septins regulate cytokinesis and multicellular development in the closest living relatives of animals
    Article Snippet: .. Clonal populations were then screened for nuclear mCherry fluorescence using a Zeiss Axio Observer.Z1/7 Widefield microscope with a Hamamatsu Orca-Flash 4.0 LT CMOS Digital Camera (Hamamatsu Photonics) (hereafter, “Zeiss Axio Observer Widefield microscope”). ..

    FISH:

    Article Title: Mast Cells Regulate Epithelial Barrier Integrity and Myeloid Immune Responses in Experimental Colitis.
    Article Snippet: .. sentative FISH images showing bacterial rRNA signal in colonic sections gnal was detected using a Cy5-labeled probe targeting bacterial ribosomal ed with 20 ×/0.8NA objective using a Zeiss Axio Observer 7 microscope. ..

    Mass Spectrometry:

    Article Title: Compartment-specific PPARδ activity controls the outcome of cancer-immune cell competition and immunotherapy efficacy.
    Article Snippet: .. MALDI mass spectrometry imaging To perform spatial metabolomics94, AKPS tumors from WT or PPARδ-iGOF mice were dissected at day 21, weighed, snap-frozen in liquid nitrogen, stored in −80 °C, and cryosectioned at 10 μm onto indium tin oxide-coated slides, with serial H&E-stained sections scanned at ×20 on a Zeiss Axio Observer 7. .. For small-molecule metabolite imaging, a 1,5-diaminonaphthalene hydrochloride matrix solution (4.3 mg ml−1 in a 4.5:5:0.5 mixture of HPLC-grade water, ethanol and 1 M hydrochloric acid, vol/vol/vol) was applied to an indium tin oxide-coated slide using a robotic sprayer (HTX Imaging) with the following parameters: four passes, 0.09 ml min−1 flow rate, 1,200 mm min−1 spray velocity, 75 °C spray nozzle temperature, 55 °C heated tray temperature, 10 psi nitrogen gas pressure, and 2-mm track spacing.

    Imaging:

    Article Title: Compartment-specific PPARδ activity controls the outcome of cancer-immune cell competition and immunotherapy efficacy.
    Article Snippet: .. MALDI mass spectrometry imaging To perform spatial metabolomics94, AKPS tumors from WT or PPARδ-iGOF mice were dissected at day 21, weighed, snap-frozen in liquid nitrogen, stored in −80 °C, and cryosectioned at 10 μm onto indium tin oxide-coated slides, with serial H&E-stained sections scanned at ×20 on a Zeiss Axio Observer 7. .. For small-molecule metabolite imaging, a 1,5-diaminonaphthalene hydrochloride matrix solution (4.3 mg ml−1 in a 4.5:5:0.5 mixture of HPLC-grade water, ethanol and 1 M hydrochloric acid, vol/vol/vol) was applied to an indium tin oxide-coated slide using a robotic sprayer (HTX Imaging) with the following parameters: four passes, 0.09 ml min−1 flow rate, 1,200 mm min−1 spray velocity, 75 °C spray nozzle temperature, 55 °C heated tray temperature, 10 psi nitrogen gas pressure, and 2-mm track spacing.

    Article Title: Cellular self-organization orchestrates ferroptosis propagation.
    Article Snippet: .. Time-lapse imaging experiments were performed using a Zeiss Axio Observer 7 inverted microscope equipped with a X-Cite Xylis (Excelitas Technologies, XT720S) LED illumination system, Definite Focus 2 system, and a Prime BSI Scientific CMOS camera (Photometrics), under controlled temperature (37 ◦C), atmosphere (5% CO2), and humidity (90%–100%) in an environmental chamber. .. Images were taken with 2 × 2 binning using a Zeiss 10x/0.45 M27 Plan-Apochromat objective.

    Fluorescence:

    Article Title: Distinct Mitochondrial Recovery Dynamics Following Taxol Removal in Triple-Negative Breast Cancer Cells.
    Article Snippet: .. Briefly, live-cell culture dishes were imaged using a wide-field fluorescence microscope (Zeiss Observer 7, White Plains, NY, USA). ..

    Article Title: Septins regulate cytokinesis and multicellular development in the closest living relatives of animals
    Article Snippet: .. Clonal populations were then screened for nuclear mCherry fluorescence using a Zeiss Axio Observer.Z1/7 Widefield microscope with a Hamamatsu Orca-Flash 4.0 LT CMOS Digital Camera (Hamamatsu Photonics) (hereafter, “Zeiss Axio Observer Widefield microscope”). ..

    Inverted Microscopy:

    Article Title: Cellular self-organization orchestrates ferroptosis propagation.
    Article Snippet: .. Time-lapse imaging experiments were performed using a Zeiss Axio Observer 7 inverted microscope equipped with a X-Cite Xylis (Excelitas Technologies, XT720S) LED illumination system, Definite Focus 2 system, and a Prime BSI Scientific CMOS camera (Photometrics), under controlled temperature (37 ◦C), atmosphere (5% CO2), and humidity (90%–100%) in an environmental chamber. .. Images were taken with 2 × 2 binning using a Zeiss 10x/0.45 M27 Plan-Apochromat objective.

    Immunofluorescence:

    Article Title: A novel approach for reliable differentiation of lymphatic endothelial precursor cells in vitro.
    Article Snippet: Afterwards, samples were immunostained with anti-mouse PDPN (127402, BioLegend), anti-mouse LYVE1 (103-PA50AG, Reliatech), and anti-TdTomato (orb182397, Byorbyt) as the primary antibodies; and Alexa Fluor 647-conjugated anti-hamster (A21451, Invitrogen), Alexa Fluor 488-conjugated anti-rabbit (A11034, Molecular Probes) and Alexa Fluor 555-conjugated anti-goat (A21432, Molecular Probes) as secondary antibodies (Table 1 3 S1). .. Immunofluorescence images were taken using Zeiss AxioObserver 7 and LSM900 confocal microscope (Zeiss). .. The expression of PDPN, SCA-1, CD90.2, CD105, CD44, CD31 (PECAM1), CD34, CD45 and CD11 of the isolated and differentiated cells was detected using the APCconjugated anti-mouse PDPN (127410, BioLegend), PE-Cy7-conjugated anti-mouse SCA-1 (558162, BioLegend), anti-mouse CD90.2 (553009, BD Biosciences), antimouse CD105 (14-1051-82, ThermoFisher), anti-mouse CD44 (553134, BD Biosciences), PE-conjugated antimouse CD31 (553373, BD Biosciences), PE-conjugated anti-mouse CD34 (551387, BD Biosciences), anti-mouse CD45 (550539, BD Biosciences) and anti-mouse CD11 (M1/70.15.11.5.2, DSHB).

    Confocal:

    Article Title: A novel approach for reliable differentiation of lymphatic endothelial precursor cells in vitro.
    Article Snippet: Afterwards, samples were immunostained with anti-mouse PDPN (127402, BioLegend), anti-mouse LYVE1 (103-PA50AG, Reliatech), and anti-TdTomato (orb182397, Byorbyt) as the primary antibodies; and Alexa Fluor 647-conjugated anti-hamster (A21451, Invitrogen), Alexa Fluor 488-conjugated anti-rabbit (A11034, Molecular Probes) and Alexa Fluor 555-conjugated anti-goat (A21432, Molecular Probes) as secondary antibodies (Table 1 3 S1). .. Immunofluorescence images were taken using Zeiss AxioObserver 7 and LSM900 confocal microscope (Zeiss). .. The expression of PDPN, SCA-1, CD90.2, CD105, CD44, CD31 (PECAM1), CD34, CD45 and CD11 of the isolated and differentiated cells was detected using the APCconjugated anti-mouse PDPN (127410, BioLegend), PE-Cy7-conjugated anti-mouse SCA-1 (558162, BioLegend), anti-mouse CD90.2 (553009, BD Biosciences), antimouse CD105 (14-1051-82, ThermoFisher), anti-mouse CD44 (553134, BD Biosciences), PE-conjugated antimouse CD31 (553373, BD Biosciences), PE-conjugated anti-mouse CD34 (551387, BD Biosciences), anti-mouse CD45 (550539, BD Biosciences) and anti-mouse CD11 (M1/70.15.11.5.2, DSHB).

    Widefield:

    Article Title: Septins regulate cytokinesis and multicellular development in the closest living relatives of animals
    Article Snippet: .. Clonal populations were then screened for nuclear mCherry fluorescence using a Zeiss Axio Observer.Z1/7 Widefield microscope with a Hamamatsu Orca-Flash 4.0 LT CMOS Digital Camera (Hamamatsu Photonics) (hereafter, “Zeiss Axio Observer Widefield microscope”). ..



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