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oligonucleotide-based comparative genomic hybridization (cgh) arrays  (Agilent technologies)


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    Agilent technologies oligonucleotide-based comparative genomic hybridization (cgh) arrays
    Oligonucleotide Based Comparative Genomic Hybridization (Cgh) Arrays, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/array-based+comparative+genomic+hybridization+(array-cgh/pmc04136899-165-20-26
    Average 90 stars, based on 1 article reviews
    oligonucleotide-based comparative genomic hybridization (cgh) arrays - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    In Vitro:

    Article Title: Mapping and mutation of the conserved DNA polymerase interaction motif (DPIM) located in the C-terminal domain of fission yeast DNA polymerase δ subunit Cdc27
    Article Snippet: This vector was then modified by addition of the S. cerevisiae LEU2 gene and S. pombe ars1 , to make pHBLA-Cdc27. .. The cdc27 + gene was then subjected to oligonucleotide-directed in vitro mutagenesis using the QuikChange method (Stratagene) with oligonucleotides CDC27-QC1 (5'-GTACGCGACAACGAAA GCTGCAgcggccgcC TTGGTTACTAAGGAAGAAG-3', with mutated sequence underlined and NotI site in lower case) and CDC27-QC2 (5'-CTTCTTCCTTAGTAACCAA GgcggccgcTGCAGC TTTCGTTGTCGCGTAC-3'), to create plasmid pHBLA-Cdc27-Q1. ..

    Mutagenesis:

    Article Title: Mapping and mutation of the conserved DNA polymerase interaction motif (DPIM) located in the C-terminal domain of fission yeast DNA polymerase δ subunit Cdc27
    Article Snippet: This vector was then modified by addition of the S. cerevisiae LEU2 gene and S. pombe ars1 , to make pHBLA-Cdc27. .. The cdc27 + gene was then subjected to oligonucleotide-directed in vitro mutagenesis using the QuikChange method (Stratagene) with oligonucleotides CDC27-QC1 (5'-GTACGCGACAACGAAA GCTGCAgcggccgcC TTGGTTACTAAGGAAGAAG-3', with mutated sequence underlined and NotI site in lower case) and CDC27-QC2 (5'-CTTCTTCCTTAGTAACCAA GgcggccgcTGCAGC TTTCGTTGTCGCGTAC-3'), to create plasmid pHBLA-Cdc27-Q1. ..

    Article Title: A Novel Variant in TPM3 Causing Muscle Weakness and Concomitant Hypercontractile Phenotype
    Article Snippet: .. The missense mutation was introduced using PCR-based oligonucleotide-directed mutagenesis kit (Agilent Technologies, Santa Clara, CA, USA) and the following forward oligonucleotide: 5′GAT CAT ATG GCT TCT ATG ATG GGC GCT ATC AAA AAA AAA ATG CAG3′ Codon that was mutated is underlined. .. The oligonucleotide was synthesized and HPLC purified by Eurofins Genomics (Ebersberg, Germany).

    Sequencing:

    Article Title: Mapping and mutation of the conserved DNA polymerase interaction motif (DPIM) located in the C-terminal domain of fission yeast DNA polymerase δ subunit Cdc27
    Article Snippet: This vector was then modified by addition of the S. cerevisiae LEU2 gene and S. pombe ars1 , to make pHBLA-Cdc27. .. The cdc27 + gene was then subjected to oligonucleotide-directed in vitro mutagenesis using the QuikChange method (Stratagene) with oligonucleotides CDC27-QC1 (5'-GTACGCGACAACGAAA GCTGCAgcggccgcC TTGGTTACTAAGGAAGAAG-3', with mutated sequence underlined and NotI site in lower case) and CDC27-QC2 (5'-CTTCTTCCTTAGTAACCAA GgcggccgcTGCAGC TTTCGTTGTCGCGTAC-3'), to create plasmid pHBLA-Cdc27-Q1. ..

    Plasmid Preparation:

    Article Title: Mapping and mutation of the conserved DNA polymerase interaction motif (DPIM) located in the C-terminal domain of fission yeast DNA polymerase δ subunit Cdc27
    Article Snippet: This vector was then modified by addition of the S. cerevisiae LEU2 gene and S. pombe ars1 , to make pHBLA-Cdc27. .. The cdc27 + gene was then subjected to oligonucleotide-directed in vitro mutagenesis using the QuikChange method (Stratagene) with oligonucleotides CDC27-QC1 (5'-GTACGCGACAACGAAA GCTGCAgcggccgcC TTGGTTACTAAGGAAGAAG-3', with mutated sequence underlined and NotI site in lower case) and CDC27-QC2 (5'-CTTCTTCCTTAGTAACCAA GgcggccgcTGCAGC TTTCGTTGTCGCGTAC-3'), to create plasmid pHBLA-Cdc27-Q1. ..

    Polymerase Chain Reaction:

    Article Title: A Novel Variant in TPM3 Causing Muscle Weakness and Concomitant Hypercontractile Phenotype
    Article Snippet: .. The missense mutation was introduced using PCR-based oligonucleotide-directed mutagenesis kit (Agilent Technologies, Santa Clara, CA, USA) and the following forward oligonucleotide: 5′GAT CAT ATG GCT TCT ATG ATG GGC GCT ATC AAA AAA AAA ATG CAG3′ Codon that was mutated is underlined. .. The oligonucleotide was synthesized and HPLC purified by Eurofins Genomics (Ebersberg, Germany).

    Microarray:

    Article Title: A translocation t(6;14) in two cases of leiomyosarcoma: Molecular cytogenetic and array-based comparative genomic hybridization characterization.
    Article Snippet: A translocation t(6;14) in two cases of leiomyosarcoma: Molecular cytogenetic and array-based comparative genomic hybridization characterization Marieke A. de Graaff , Daniëlle de Jong , Inge H. Briaire-de Bruijn , Pancras C.W.. Hogendoorn , Judith V.M.G.. Bovée , Károly Szuhai * a Department of Pathology, Leiden University Medical Center (LUMC), Leiden, The Netherlands; b Department of Molecular Cell Biology, LUMC, Leiden, The Netherlands

    Formalin-fixed Paraffin-Embedded:

    Article Title: Mitotic index, microvascular proliferation, and necrosis define 3 groups of 1p/19q codeleted anaplastic oligodendrogliomas associated with different genomic alterations
    Article Snippet: Two types of platforms were used: HumanCNV370-Quad and Human610-Quad from Illumina. .. 14 When only DNA extracted from FFPE tissue was available ( n = 23), the genomic profile was assessed using oligonucleotide-based comparative genomic hybridization (CGH) arrays (Agilent). ..

    Article Title: CDKN2A homozygous deletion is a strong adverse prognosis factor in diffuse malignant IDH -mutant gliomas
    Article Snippet: Two types of platforms were used: HumanCNV370-Quad and Human610-Quad from Illumina. .. When only DNA extracted from formalin-fixed paraffin embedded tissue was available, CDKN2A homozygous deletion was assessed using oligonucleotide-based CGH arrays (Agilent, n = 111) or next generation sequencing (NGS) (BrainCap Custom Capture from Roche, n = 227). ..

    Hybridization:

    Article Title: Mitotic index, microvascular proliferation, and necrosis define 3 groups of 1p/19q codeleted anaplastic oligodendrogliomas associated with different genomic alterations
    Article Snippet: Two types of platforms were used: HumanCNV370-Quad and Human610-Quad from Illumina. .. 14 When only DNA extracted from FFPE tissue was available ( n = 23), the genomic profile was assessed using oligonucleotide-based comparative genomic hybridization (CGH) arrays (Agilent). ..

    other:

    Article Title: The Lipid Metabolism as Target and Modulator of BOLD-100 Anticancer Activity: Crosstalk with Histone Acetylation.
    Article Snippet: Whole genome gene expression analyses were performed on 4 × 44 K oligonucleotide-based microarrays (G4845A, Agilent Technologies, Santa Clara, CA, USA) according to manufacturer’s instructions.

    Article Title: Tool for diagnosis and prognosis of mature B-cell neoplasms
    Article Snippet: In MatBA array-CGH, the oligonucleotide-based microarray is designed by CGI (using eArray by Agilent Technologies) and manufactured by Agilent.

    Article Title: Parental Imbalances Involving Chromosomes 15q and 22q May Predispose to the Formation of De Novo Pathogenic Microdeletions and Microduplications in the Offspring
    Article Snippet: To confirm the results of array-CGH, fluorescence in situ hybridization (FISH) experiments were performed on metaphase chromosomes of mother and daughter of family 1 with the following probes: subtelomeric Tel7qter probe (TelVysion 7q SpectrumOrange; Vysis/Abbott, Inc., Downers Grove, IL), oligonucleotide-based probe 15q13.3 (chr15∶31,775,207-31,899,230) (SureFISH G100005G, Agilent Technologies), BAC probe RP11-231A23 (chr15∶58,181,400- 58,324,747 )(Bluegnome, Cambridge, UK) mapping at 15q22.2.

    Next-Generation Sequencing:

    Article Title: CDKN2A homozygous deletion is a strong adverse prognosis factor in diffuse malignant IDH -mutant gliomas
    Article Snippet: Two types of platforms were used: HumanCNV370-Quad and Human610-Quad from Illumina. .. When only DNA extracted from formalin-fixed paraffin embedded tissue was available, CDKN2A homozygous deletion was assessed using oligonucleotide-based CGH arrays (Agilent, n = 111) or next generation sequencing (NGS) (BrainCap Custom Capture from Roche, n = 227). ..



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