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customized rt2 profiler pcr array gene expression kit  (Qiagen)


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    Qiagen customized rt2 profiler pcr array gene expression kit
    Cross-talk between CoV-2-infected epithelial cells and immune cells activated MAIT and exacerbated inflammation. (A) Heatmap of immunological profile of Caco-2-ACE2-N cells infected with CoV-2 and PBMC cocultured with CoV-2-infected Caco-2-ACE2-N cells. mRNA fold change of immune genes was profiled by <t>RT2</t> Profiler <t>PCR</t> Array Gene Expression Analysis. Left panel indicated group of CoV-2-infected Caco-2-ACE2-N cells, shortly named with “Caco-2 ACE2-N”; data were presented with mRNA fold change relative to Mock-treated Caco-2-ACE2-N cells. Right panel indicated group of PBMCs cocultured with CoV-2-infected Caco-2-ACE2-N cells, shortly named with “PBMC”; data were presented with mRNA fold change relative to control PBMCs cocultured with Mock-treated Caco-2-ACE2-N cells. (B) CD69 expression profile on MAIT cells in various coculture groups. “Isotype ctr” indicated isotype antibody staining; “Mock” group indicated MAIT cells cocultured with Mock-treated IECs (Caco-2-ACE-2-N); “CoV-2” group indicated MAIT cells cocultured with CoV-2-infected IECs. (C) Representative flow cytometry dot plot of IFNγ expression in NK and MAIT cells after coculture with either Mock or CoV-2 infected IECs (Caco-2-ACE-2-N). (D) Inflammatory cytokine (IFNγ, GrzB, TNFα) expression in MAIT cells determined by FACS with positive population percentage. “Mock” indicated group of MAIT cells cocultured with Mock-treated IECs; “CoV-2” indicated group of MAIT cells cocultured with CoV-2-infected IECs (E) Depletion of IL18 by adding αIL18 Ab into medium at the beginning of coculture. Cytokine expression in MAIT with depletion of IL18 in coculture or with rIL18 addition alone in PBMC culture medium. All data are represented as mean ± SEM of three replicates. Statistical significance is indicated. (ns, not significant, **p<0.01 ***p<0.001, unpaired t test or One-way ANOVA).
    Customized Rt2 Profiler Pcr Array Gene Expression Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/array+gene+expression+profiling/rt2+profiler+pcr+array/pmc09941539-222-11-18
    Average 90 stars, based on 1 article reviews
    customized rt2 profiler pcr array gene expression kit - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "SARS-CoV-2 infection of intestinal epithelia cells sensed by RIG-I and DHX-15 evokes innate immune response and immune cross-talk"

    Article Title: SARS-CoV-2 infection of intestinal epithelia cells sensed by RIG-I and DHX-15 evokes innate immune response and immune cross-talk

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2022.1035711

    Cross-talk between CoV-2-infected epithelial cells and immune cells activated MAIT and exacerbated inflammation. (A) Heatmap of immunological profile of Caco-2-ACE2-N cells infected with CoV-2 and PBMC cocultured with CoV-2-infected Caco-2-ACE2-N cells. mRNA fold change of immune genes was profiled by RT2 Profiler PCR Array Gene Expression Analysis. Left panel indicated group of CoV-2-infected Caco-2-ACE2-N cells, shortly named with “Caco-2 ACE2-N”; data were presented with mRNA fold change relative to Mock-treated Caco-2-ACE2-N cells. Right panel indicated group of PBMCs cocultured with CoV-2-infected Caco-2-ACE2-N cells, shortly named with “PBMC”; data were presented with mRNA fold change relative to control PBMCs cocultured with Mock-treated Caco-2-ACE2-N cells. (B) CD69 expression profile on MAIT cells in various coculture groups. “Isotype ctr” indicated isotype antibody staining; “Mock” group indicated MAIT cells cocultured with Mock-treated IECs (Caco-2-ACE-2-N); “CoV-2” group indicated MAIT cells cocultured with CoV-2-infected IECs. (C) Representative flow cytometry dot plot of IFNγ expression in NK and MAIT cells after coculture with either Mock or CoV-2 infected IECs (Caco-2-ACE-2-N). (D) Inflammatory cytokine (IFNγ, GrzB, TNFα) expression in MAIT cells determined by FACS with positive population percentage. “Mock” indicated group of MAIT cells cocultured with Mock-treated IECs; “CoV-2” indicated group of MAIT cells cocultured with CoV-2-infected IECs (E) Depletion of IL18 by adding αIL18 Ab into medium at the beginning of coculture. Cytokine expression in MAIT with depletion of IL18 in coculture or with rIL18 addition alone in PBMC culture medium. All data are represented as mean ± SEM of three replicates. Statistical significance is indicated. (ns, not significant, **p<0.01 ***p<0.001, unpaired t test or One-way ANOVA).
    Figure Legend Snippet: Cross-talk between CoV-2-infected epithelial cells and immune cells activated MAIT and exacerbated inflammation. (A) Heatmap of immunological profile of Caco-2-ACE2-N cells infected with CoV-2 and PBMC cocultured with CoV-2-infected Caco-2-ACE2-N cells. mRNA fold change of immune genes was profiled by RT2 Profiler PCR Array Gene Expression Analysis. Left panel indicated group of CoV-2-infected Caco-2-ACE2-N cells, shortly named with “Caco-2 ACE2-N”; data were presented with mRNA fold change relative to Mock-treated Caco-2-ACE2-N cells. Right panel indicated group of PBMCs cocultured with CoV-2-infected Caco-2-ACE2-N cells, shortly named with “PBMC”; data were presented with mRNA fold change relative to control PBMCs cocultured with Mock-treated Caco-2-ACE2-N cells. (B) CD69 expression profile on MAIT cells in various coculture groups. “Isotype ctr” indicated isotype antibody staining; “Mock” group indicated MAIT cells cocultured with Mock-treated IECs (Caco-2-ACE-2-N); “CoV-2” group indicated MAIT cells cocultured with CoV-2-infected IECs. (C) Representative flow cytometry dot plot of IFNγ expression in NK and MAIT cells after coculture with either Mock or CoV-2 infected IECs (Caco-2-ACE-2-N). (D) Inflammatory cytokine (IFNγ, GrzB, TNFα) expression in MAIT cells determined by FACS with positive population percentage. “Mock” indicated group of MAIT cells cocultured with Mock-treated IECs; “CoV-2” indicated group of MAIT cells cocultured with CoV-2-infected IECs (E) Depletion of IL18 by adding αIL18 Ab into medium at the beginning of coculture. Cytokine expression in MAIT with depletion of IL18 in coculture or with rIL18 addition alone in PBMC culture medium. All data are represented as mean ± SEM of three replicates. Statistical significance is indicated. (ns, not significant, **p<0.01 ***p<0.001, unpaired t test or One-way ANOVA).

    Techniques Used: Infection, Gene Expression, Control, Expressing, Staining, Flow Cytometry

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Evidence for a JAK2/STAT3 proinflammatory and vasculogenic mimicry interrelated molecular signature in adipocyte-derived mesenchymal stromal/stem cells
    Article Snippet: .. Premade RT 2 Profiler PCR arrays for Human Inflammatory Cytokines and Receptors (PAHS-011ZD) were purchased from QIAGEN and used following the manufacturer’s instructions. .. Briefly, the genomic DNA was removed before 0.5 μg of total RNA was reverse transcribed via the RT 2 First Strand Kit (QIAGEN, 330404).

    Article Title: Long non-coding RNA CHROMR regulates antiviral immunity in humans
    Article Snippet: .. RNA was isolated from THP-1 macrophages stable overexpressing CHROMR or an empty vector control, reverse-transcribed, and qPCR analysis of Type I Interferon Response genes was performed using RT2 Profiler PCR Arrays (Qiagen, PAHS-016ZA) according to manufacturer's protocol. ..

    Article Title: Evidence for a JAK2/STAT3 proinflammatory and vasculogenic mimicry interrelated molecular signature in adipocyte-derived mesenchymal stromal/stem cells.
    Article Snippet: .. Premade RT2 Profiler PCR arrays for Human Inflammatory Cytokines and Receptors (PAHS-011ZD) were purchased from QIAGEN and used following the manufacturer’s instructions. .. Briefly, the genomic DNA was removed before 0.5 μg of total RNA was reverse transcribed via the RT2 First Strand Kit (QIAGEN, 330404).

    Article Title: Mouse Model of Sutured Endothelial Keratoplasty Technique.
    Article Snippet: Following mRNA extraction, cDNA was prepared using the RT2 First Strand Kit (QIAGEN, Hilden, Germany). .. The PCR array method was used for the simultaneous analysis of multiple gene expressions using RT2 Profiler PCR Arrays (QIAGEN). .. The RT2 Profiler PCR Array for Mouse Innate & Adaptive Immune Responses (PAMM-052ZA, QIAGEN) was used for this experiment.

    Article Title:
    Article Snippet: .. Collagen I (2D model) IPF CDM (3D model)a b Supplementary Fig.6 | Gene expression of IPF KRT5+ cells compared to healthy KRT5+ cells cultured on collagen I or IPF CDMs. a,b Volcano plots showing gene expression changes determined by Qiagen RT2 PCR Array between KRT5+ cells from IPF patients (n = 3) vs. healthy controls (n = 3) cultured on a collagen I or b IPF CDMs. ..

    Article Title: Metabolic reprogramming during ineffective erythropoiesis in β-thalassemia/HbE disease.
    Article Snippet: The cDNA was mixed with RT2 SYBR Green qPCR Mastermix (QIAGEN, cat. No. 330500). .. The metabolic gene expression profiles were determined using an RT2 Profiler PCR array with a 96-well plate format coated with specific primers and probes of 84 glucose metabolic genes (Qiagen). .. The PCR array plate was subjected to a real-time PCR machine (Biorad, CFX 96).

    Article Title:
    Article Snippet: .. To test the gene expression of 84 genes involved in DNA damage signaling pathways, the RT2 ProfilerTM PCR Array (Mouse DNA Damage Signaling Pathway from Qiagen) was used and performed as described in the manufacturer's protocol. .. Initially, cDNA was prepared using RT2 First Strand Kit (Qiagen) according to the manufacturer's protocol.

    Article Title: The potassium channel K 2P 2.1 shapes the morphology and function of brain endothelial cells via actin network remodeling
    Article Snippet: .. For analyzing the transcriptome with RT 2 Profiler PCR array (Qiagen), RT 2 First Stand Kit (Qiagen) was used for cDNA synthesis. .. For analysis of transcripts related to cytoskeletal regulation, cDNA was prepared for quantitative real time PCR (qRT-PCR) using the RT 2 SYBR Green qPCR Mastermix (Qiagen) and loaded onto plates from the RT 2 Profiler PCR Array Mouse Cytoskeleton Regulators (Qiagen, PAMM-088Z).

    Isolation:

    Article Title: Long non-coding RNA CHROMR regulates antiviral immunity in humans
    Article Snippet: .. RNA was isolated from THP-1 macrophages stable overexpressing CHROMR or an empty vector control, reverse-transcribed, and qPCR analysis of Type I Interferon Response genes was performed using RT2 Profiler PCR Arrays (Qiagen, PAHS-016ZA) according to manufacturer's protocol. ..

    Plasmid Preparation:

    Article Title: Long non-coding RNA CHROMR regulates antiviral immunity in humans
    Article Snippet: .. RNA was isolated from THP-1 macrophages stable overexpressing CHROMR or an empty vector control, reverse-transcribed, and qPCR analysis of Type I Interferon Response genes was performed using RT2 Profiler PCR Arrays (Qiagen, PAHS-016ZA) according to manufacturer's protocol. ..

    Control:

    Article Title: Long non-coding RNA CHROMR regulates antiviral immunity in humans
    Article Snippet: .. RNA was isolated from THP-1 macrophages stable overexpressing CHROMR or an empty vector control, reverse-transcribed, and qPCR analysis of Type I Interferon Response genes was performed using RT2 Profiler PCR Arrays (Qiagen, PAHS-016ZA) according to manufacturer's protocol. ..

    Reverse Transcription:

    Article Title: Long non-coding RNA CHROMR regulates antiviral immunity in humans
    Article Snippet: .. RNA was isolated from THP-1 macrophages stable overexpressing CHROMR or an empty vector control, reverse-transcribed, and qPCR analysis of Type I Interferon Response genes was performed using RT2 Profiler PCR Arrays (Qiagen, PAHS-016ZA) according to manufacturer's protocol. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Long non-coding RNA CHROMR regulates antiviral immunity in humans
    Article Snippet: .. RNA was isolated from THP-1 macrophages stable overexpressing CHROMR or an empty vector control, reverse-transcribed, and qPCR analysis of Type I Interferon Response genes was performed using RT2 Profiler PCR Arrays (Qiagen, PAHS-016ZA) according to manufacturer's protocol. ..

    Gene Expression:

    Article Title:
    Article Snippet: .. Collagen I (2D model) IPF CDM (3D model)a b Supplementary Fig.6 | Gene expression of IPF KRT5+ cells compared to healthy KRT5+ cells cultured on collagen I or IPF CDMs. a,b Volcano plots showing gene expression changes determined by Qiagen RT2 PCR Array between KRT5+ cells from IPF patients (n = 3) vs. healthy controls (n = 3) cultured on a collagen I or b IPF CDMs. ..

    Article Title: Metabolic reprogramming during ineffective erythropoiesis in β-thalassemia/HbE disease.
    Article Snippet: The cDNA was mixed with RT2 SYBR Green qPCR Mastermix (QIAGEN, cat. No. 330500). .. The metabolic gene expression profiles were determined using an RT2 Profiler PCR array with a 96-well plate format coated with specific primers and probes of 84 glucose metabolic genes (Qiagen). .. The PCR array plate was subjected to a real-time PCR machine (Biorad, CFX 96).

    Article Title:
    Article Snippet: .. To test the gene expression of 84 genes involved in DNA damage signaling pathways, the RT2 ProfilerTM PCR Array (Mouse DNA Damage Signaling Pathway from Qiagen) was used and performed as described in the manufacturer's protocol. .. Initially, cDNA was prepared using RT2 First Strand Kit (Qiagen) according to the manufacturer's protocol.

    Cell Culture:

    Article Title:
    Article Snippet: .. Collagen I (2D model) IPF CDM (3D model)a b Supplementary Fig.6 | Gene expression of IPF KRT5+ cells compared to healthy KRT5+ cells cultured on collagen I or IPF CDMs. a,b Volcano plots showing gene expression changes determined by Qiagen RT2 PCR Array between KRT5+ cells from IPF patients (n = 3) vs. healthy controls (n = 3) cultured on a collagen I or b IPF CDMs. ..

    Protein-Protein interactions:

    Article Title:
    Article Snippet: .. To test the gene expression of 84 genes involved in DNA damage signaling pathways, the RT2 ProfilerTM PCR Array (Mouse DNA Damage Signaling Pathway from Qiagen) was used and performed as described in the manufacturer's protocol. .. Initially, cDNA was prepared using RT2 First Strand Kit (Qiagen) according to the manufacturer's protocol.

    cDNA Synthesis:

    Article Title: The potassium channel K 2P 2.1 shapes the morphology and function of brain endothelial cells via actin network remodeling
    Article Snippet: .. For analyzing the transcriptome with RT 2 Profiler PCR array (Qiagen), RT 2 First Stand Kit (Qiagen) was used for cDNA synthesis. .. For analysis of transcripts related to cytoskeletal regulation, cDNA was prepared for quantitative real time PCR (qRT-PCR) using the RT 2 SYBR Green qPCR Mastermix (Qiagen) and loaded onto plates from the RT 2 Profiler PCR Array Mouse Cytoskeleton Regulators (Qiagen, PAMM-088Z).



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    Cross-talk between CoV-2-infected epithelial cells and immune cells activated MAIT and exacerbated inflammation. (A) Heatmap of immunological profile of Caco-2-ACE2-N cells infected with CoV-2 and PBMC cocultured with CoV-2-infected Caco-2-ACE2-N cells. mRNA fold change of immune genes was profiled by <t>RT2</t> Profiler <t>PCR</t> Array Gene Expression Analysis. Left panel indicated group of CoV-2-infected Caco-2-ACE2-N cells, shortly named with “Caco-2 ACE2-N”; data were presented with mRNA fold change relative to Mock-treated Caco-2-ACE2-N cells. Right panel indicated group of PBMCs cocultured with CoV-2-infected Caco-2-ACE2-N cells, shortly named with “PBMC”; data were presented with mRNA fold change relative to control PBMCs cocultured with Mock-treated Caco-2-ACE2-N cells. (B) CD69 expression profile on MAIT cells in various coculture groups. “Isotype ctr” indicated isotype antibody staining; “Mock” group indicated MAIT cells cocultured with Mock-treated IECs (Caco-2-ACE-2-N); “CoV-2” group indicated MAIT cells cocultured with CoV-2-infected IECs. (C) Representative flow cytometry dot plot of IFNγ expression in NK and MAIT cells after coculture with either Mock or CoV-2 infected IECs (Caco-2-ACE-2-N). (D) Inflammatory cytokine (IFNγ, GrzB, TNFα) expression in MAIT cells determined by FACS with positive population percentage. “Mock” indicated group of MAIT cells cocultured with Mock-treated IECs; “CoV-2” indicated group of MAIT cells cocultured with CoV-2-infected IECs (E) Depletion of IL18 by adding αIL18 Ab into medium at the beginning of coculture. Cytokine expression in MAIT with depletion of IL18 in coculture or with rIL18 addition alone in PBMC culture medium. All data are represented as mean ± SEM of three replicates. Statistical significance is indicated. (ns, not significant, **p<0.01 ***p<0.001, unpaired t test or One-way ANOVA).
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    Image Search Results


    Genes analyzed with the RT 2 Profiler PCR Array—Human DNA Damage Signaling Pathway kit (Qiagen, Germantown, MD, USA).

    Journal: Current Issues in Molecular Biology

    Article Title: DNA Double-Strand Break Repair Inhibitors: YU238259, A12B4C3 and DDRI-18 Overcome the Cisplatin Resistance in Human Ovarian Cancer Cells, but Not under Hypoxia Conditions

    doi: 10.3390/cimb45100500

    Figure Lengend Snippet: Genes analyzed with the RT 2 Profiler PCR Array—Human DNA Damage Signaling Pathway kit (Qiagen, Germantown, MD, USA).

    Article Snippet: Expression of genes related to cell response to DNA damage was carried out using Real-time PCR using the RT 2 Profiler PCR Array—Human DNA Damage Signaling Pathway gene expression evaluation kit (Qiagen, Germantown, MD, USA).

    Techniques:

    Cross-talk between CoV-2-infected epithelial cells and immune cells activated MAIT and exacerbated inflammation. (A) Heatmap of immunological profile of Caco-2-ACE2-N cells infected with CoV-2 and PBMC cocultured with CoV-2-infected Caco-2-ACE2-N cells. mRNA fold change of immune genes was profiled by RT2 Profiler PCR Array Gene Expression Analysis. Left panel indicated group of CoV-2-infected Caco-2-ACE2-N cells, shortly named with “Caco-2 ACE2-N”; data were presented with mRNA fold change relative to Mock-treated Caco-2-ACE2-N cells. Right panel indicated group of PBMCs cocultured with CoV-2-infected Caco-2-ACE2-N cells, shortly named with “PBMC”; data were presented with mRNA fold change relative to control PBMCs cocultured with Mock-treated Caco-2-ACE2-N cells. (B) CD69 expression profile on MAIT cells in various coculture groups. “Isotype ctr” indicated isotype antibody staining; “Mock” group indicated MAIT cells cocultured with Mock-treated IECs (Caco-2-ACE-2-N); “CoV-2” group indicated MAIT cells cocultured with CoV-2-infected IECs. (C) Representative flow cytometry dot plot of IFNγ expression in NK and MAIT cells after coculture with either Mock or CoV-2 infected IECs (Caco-2-ACE-2-N). (D) Inflammatory cytokine (IFNγ, GrzB, TNFα) expression in MAIT cells determined by FACS with positive population percentage. “Mock” indicated group of MAIT cells cocultured with Mock-treated IECs; “CoV-2” indicated group of MAIT cells cocultured with CoV-2-infected IECs (E) Depletion of IL18 by adding αIL18 Ab into medium at the beginning of coculture. Cytokine expression in MAIT with depletion of IL18 in coculture or with rIL18 addition alone in PBMC culture medium. All data are represented as mean ± SEM of three replicates. Statistical significance is indicated. (ns, not significant, **p<0.01 ***p<0.001, unpaired t test or One-way ANOVA).

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: SARS-CoV-2 infection of intestinal epithelia cells sensed by RIG-I and DHX-15 evokes innate immune response and immune cross-talk

    doi: 10.3389/fcimb.2022.1035711

    Figure Lengend Snippet: Cross-talk between CoV-2-infected epithelial cells and immune cells activated MAIT and exacerbated inflammation. (A) Heatmap of immunological profile of Caco-2-ACE2-N cells infected with CoV-2 and PBMC cocultured with CoV-2-infected Caco-2-ACE2-N cells. mRNA fold change of immune genes was profiled by RT2 Profiler PCR Array Gene Expression Analysis. Left panel indicated group of CoV-2-infected Caco-2-ACE2-N cells, shortly named with “Caco-2 ACE2-N”; data were presented with mRNA fold change relative to Mock-treated Caco-2-ACE2-N cells. Right panel indicated group of PBMCs cocultured with CoV-2-infected Caco-2-ACE2-N cells, shortly named with “PBMC”; data were presented with mRNA fold change relative to control PBMCs cocultured with Mock-treated Caco-2-ACE2-N cells. (B) CD69 expression profile on MAIT cells in various coculture groups. “Isotype ctr” indicated isotype antibody staining; “Mock” group indicated MAIT cells cocultured with Mock-treated IECs (Caco-2-ACE-2-N); “CoV-2” group indicated MAIT cells cocultured with CoV-2-infected IECs. (C) Representative flow cytometry dot plot of IFNγ expression in NK and MAIT cells after coculture with either Mock or CoV-2 infected IECs (Caco-2-ACE-2-N). (D) Inflammatory cytokine (IFNγ, GrzB, TNFα) expression in MAIT cells determined by FACS with positive population percentage. “Mock” indicated group of MAIT cells cocultured with Mock-treated IECs; “CoV-2” indicated group of MAIT cells cocultured with CoV-2-infected IECs (E) Depletion of IL18 by adding αIL18 Ab into medium at the beginning of coculture. Cytokine expression in MAIT with depletion of IL18 in coculture or with rIL18 addition alone in PBMC culture medium. All data are represented as mean ± SEM of three replicates. Statistical significance is indicated. (ns, not significant, **p<0.01 ***p<0.001, unpaired t test or One-way ANOVA).

    Article Snippet: After that, PBMC were harvested for immune genes profiling with customized RT2 Profiler PCR Array Gene Expression Kit (Qiagen).

    Techniques: Infection, Gene Expression, Control, Expressing, Staining, Flow Cytometry

    Quality of human ORF features on GMAP chip . (a) Intensity signals from the GMAP array (X-axis) plotted against signals from the Human Gene 1.0 ST array (Y-axis) following amplification of human ORFeome v5.1 pools and hybridization of the resulting probe to each of these arrays. Common features between the two arrays were used to calculate the Pearson correlation coefficient. (b) Distribution of signal intensities from replicate GMAP or Human 1.0 ST arrays as described in (a) for shared features. Hybridization behaviour for the huORF features compared to the (c) huGene features or the (d) 22-mer hairpin features for corresponding genes.

    Journal: BMC Genomics

    Article Title: A comprehensive platform for highly multiplexed mammalian functional genetic screens

    doi: 10.1186/1471-2164-12-213

    Figure Lengend Snippet: Quality of human ORF features on GMAP chip . (a) Intensity signals from the GMAP array (X-axis) plotted against signals from the Human Gene 1.0 ST array (Y-axis) following amplification of human ORFeome v5.1 pools and hybridization of the resulting probe to each of these arrays. Common features between the two arrays were used to calculate the Pearson correlation coefficient. (b) Distribution of signal intensities from replicate GMAP or Human 1.0 ST arrays as described in (a) for shared features. Hybridization behaviour for the huORF features compared to the (c) huGene features or the (d) 22-mer hairpin features for corresponding genes.

    Article Snippet: For comparative purposes, we also included up to three additional features found on the human expression profiling Affymetrix Gene 1.0 ST array for 18,088 of these sequences.

    Techniques: Amplification, Hybridization