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rt 2 profiler pcr array—human dna damage signaling pathway gene expression evaluation kit  (Qiagen)


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    Qiagen rt 2 profiler pcr array—human dna damage signaling pathway gene expression evaluation kit
    Genes analyzed with the <t> RT 2 Profiler PCR Array—Human </t> DNA Damage Signaling Pathway kit (Qiagen, Germantown, MD, USA).
    Rt 2 Profiler Pcr Array—Human Dna Damage Signaling Pathway Gene Expression Evaluation Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/array+gene+expression+profiling/rt2+profiler+pcr+array+human+dna+repair/pmc10605129-209-18-31
    Average 90 stars, based on 1 article reviews
    rt 2 profiler pcr array—human dna damage signaling pathway gene expression evaluation kit - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "DNA Double-Strand Break Repair Inhibitors: YU238259, A12B4C3 and DDRI-18 Overcome the Cisplatin Resistance in Human Ovarian Cancer Cells, but Not under Hypoxia Conditions"

    Article Title: DNA Double-Strand Break Repair Inhibitors: YU238259, A12B4C3 and DDRI-18 Overcome the Cisplatin Resistance in Human Ovarian Cancer Cells, but Not under Hypoxia Conditions

    Journal: Current Issues in Molecular Biology

    doi: 10.3390/cimb45100500

    Genes analyzed with the  RT 2 Profiler PCR Array—Human  DNA Damage Signaling Pathway kit (Qiagen, Germantown, MD, USA).
    Figure Legend Snippet: Genes analyzed with the RT 2 Profiler PCR Array—Human DNA Damage Signaling Pathway kit (Qiagen, Germantown, MD, USA).

    Techniques Used:

    Related Articles

    Polymerase Chain Reaction:

    Article Title: A Novel DNA Repair‐Gene Model to Predict Responses to Immunotherapy and Prognosis in Patients With EGFR ‐Mutant Non‐Small Cell Lung Cancer
    Article Snippet: .. A total of 84 DDR genes were obtained from GeneGlobe Design & Analysis Hub: RT 2 Profiler PCR Array Human DNA Damage Signaling Pathway ( https://geneglobe.qiagen.com/ro/product‐groups/rt2‐profiler‐pcr‐arrays/PAHS‐029Z ). ..

    Article Title: Interplay between MycN and c-Myc regulates radioresistance and cancer stem cell phenotype in neuroblastoma upon glutamine deprivation.
    Article Snippet: .. Expression levels of 84 DNA repair genes were analysed using RT2 Profiler PCR Array Human DNA Repair (Qiagen, #PAHS-042Z) according to the manufacturer’s recommendations. .. Expression of 84 CSC-related genes was analysed by RT2 Profiler PCR Array Human Cancer Stem Cells (Qiagen, #PAHS-176Z) according to the manufacturer’s recommendations.

    Article Title: DNA Double-Strand Break Repair Inhibitors: YU238259, A12B4C3 and DDRI-18 Overcome the Cisplatin Resistance in Human Ovarian Cancer Cells, but Not under Hypoxia Conditions
    Article Snippet: RNA was obtained from cells cultured under aerobic and hypoxic conditions. cDNA synthesis on the mRNA template was performed using the RT2 HT First Strand Kit (Qiagen, Germantown, MD, USA). .. Expression of genes related to cell response to DNA damage was carried out using Real-time PCR using the RT 2 Profiler PCR Array—Human DNA Damage Signaling Pathway gene expression evaluation kit (Qiagen, Germantown, MD, USA). ..

    Article Title: The Curcumin Analog PAC Is a Potential Solution for the Treatment of Triple-Negative Breast Cancer by Modulating the Gene Expression of DNA Repair Pathways
    Article Snippet: .. To determine whether PAC modulates DNA repair pathways, real-time PCR was performed using RT 2 Profiler PCR Array Human DNA Repair (PAHS-042Z) from Qiagen according to manufacturer instructions [ , ]. ..

    Article Title: PARP Inhibitors Effectively Reduce MAPK Inhibitor Resistant Melanoma Cell Growth and Synergize with MAPK Inhibitors through a Synthetic Lethal Interaction In Vitro and In Vivo
    Article Snippet: .. RT 2 Profiler PCR Array Human DNA Repair and p53 Signaling Pathway (Qiagen PAHS-042) were used. ..

    Expressing:

    Article Title: Interplay between MycN and c-Myc regulates radioresistance and cancer stem cell phenotype in neuroblastoma upon glutamine deprivation.
    Article Snippet: .. Expression levels of 84 DNA repair genes were analysed using RT2 Profiler PCR Array Human DNA Repair (Qiagen, #PAHS-042Z) according to the manufacturer’s recommendations. .. Expression of 84 CSC-related genes was analysed by RT2 Profiler PCR Array Human Cancer Stem Cells (Qiagen, #PAHS-176Z) according to the manufacturer’s recommendations.

    Article Title: DNA Double-Strand Break Repair Inhibitors: YU238259, A12B4C3 and DDRI-18 Overcome the Cisplatin Resistance in Human Ovarian Cancer Cells, but Not under Hypoxia Conditions
    Article Snippet: RNA was obtained from cells cultured under aerobic and hypoxic conditions. cDNA synthesis on the mRNA template was performed using the RT2 HT First Strand Kit (Qiagen, Germantown, MD, USA). .. Expression of genes related to cell response to DNA damage was carried out using Real-time PCR using the RT 2 Profiler PCR Array—Human DNA Damage Signaling Pathway gene expression evaluation kit (Qiagen, Germantown, MD, USA). ..

    other:

    Article Title: Dysregulation of DNA repair genes in Fuchs endothelial corneal dystrophy.
    Article Snippet: Fuchs Endothelial Corneal Dystrophy (FECD), a late-onset oxidative stress disorder, is the most common cause of corneal endothelial degeneration and is genetically associated with CTG repeat expansion in Transcription Factor 4 (TCF4).. We previously reported accumulation of nuclear (nDNA) and mitochondrial (mtDNA) damage in FECD.. Specifically, mtDNA damage was a prominent finding in development of disease in the ultraviolet-A (UVA) induced FECD mouse model. We hypothesize that an aberrant DNA repair may contribute to the increased DNA damage seen in FECD.

    Article Title: Oct4 confers stemness and radioresistance to head and neck squamous cell carcinoma by regulating the homologous recombination factors PSMC3IP and RAD54L
    Article Snippet: Gene expression 187 analysis of 83 DNA repair genes was performed with the RT2 Profiler PCR Array Human 188 DNA Repair (Qiagen, #PAHS-042Z) according to the manufacturer's instructions.

    Article Title: Combinatorial Effect of PLK1 Inhibition with Temozolomide and Radiation in Glioblastoma
    Article Snippet: LN229 cells were collected after 24 h of treatment followed by the release of drugs for additional 24 h. RNA was extracted by RNeasy plus mini kit (Qiagen, Cat:74134, Hilden, Germany) and cDNA was prepared by RT2 First Strand Kit (Qiagen, Cat:33041).

    Real-time Polymerase Chain Reaction:

    Article Title: DNA Double-Strand Break Repair Inhibitors: YU238259, A12B4C3 and DDRI-18 Overcome the Cisplatin Resistance in Human Ovarian Cancer Cells, but Not under Hypoxia Conditions
    Article Snippet: RNA was obtained from cells cultured under aerobic and hypoxic conditions. cDNA synthesis on the mRNA template was performed using the RT2 HT First Strand Kit (Qiagen, Germantown, MD, USA). .. Expression of genes related to cell response to DNA damage was carried out using Real-time PCR using the RT 2 Profiler PCR Array—Human DNA Damage Signaling Pathway gene expression evaluation kit (Qiagen, Germantown, MD, USA). ..

    Article Title: The Curcumin Analog PAC Is a Potential Solution for the Treatment of Triple-Negative Breast Cancer by Modulating the Gene Expression of DNA Repair Pathways
    Article Snippet: .. To determine whether PAC modulates DNA repair pathways, real-time PCR was performed using RT 2 Profiler PCR Array Human DNA Repair (PAHS-042Z) from Qiagen according to manufacturer instructions [ , ]. ..

    Gene Expression:

    Article Title: DNA Double-Strand Break Repair Inhibitors: YU238259, A12B4C3 and DDRI-18 Overcome the Cisplatin Resistance in Human Ovarian Cancer Cells, but Not under Hypoxia Conditions
    Article Snippet: RNA was obtained from cells cultured under aerobic and hypoxic conditions. cDNA synthesis on the mRNA template was performed using the RT2 HT First Strand Kit (Qiagen, Germantown, MD, USA). .. Expression of genes related to cell response to DNA damage was carried out using Real-time PCR using the RT 2 Profiler PCR Array—Human DNA Damage Signaling Pathway gene expression evaluation kit (Qiagen, Germantown, MD, USA). ..



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    Image Search Results


    Genes analyzed with the  RT 2 Profiler PCR Array—Human  DNA Damage Signaling Pathway kit (Qiagen, Germantown, MD, USA).

    Journal: Current Issues in Molecular Biology

    Article Title: DNA Double-Strand Break Repair Inhibitors: YU238259, A12B4C3 and DDRI-18 Overcome the Cisplatin Resistance in Human Ovarian Cancer Cells, but Not under Hypoxia Conditions

    doi: 10.3390/cimb45100500

    Figure Lengend Snippet: Genes analyzed with the RT 2 Profiler PCR Array—Human DNA Damage Signaling Pathway kit (Qiagen, Germantown, MD, USA).

    Article Snippet: Expression of genes related to cell response to DNA damage was carried out using Real-time PCR using the RT 2 Profiler PCR Array—Human DNA Damage Signaling Pathway gene expression evaluation kit (Qiagen, Germantown, MD, USA).

    Techniques:

    Cross-talk between CoV-2-infected epithelial cells and immune cells activated MAIT and exacerbated inflammation. (A) Heatmap of immunological profile of Caco-2-ACE2-N cells infected with CoV-2 and PBMC cocultured with CoV-2-infected Caco-2-ACE2-N cells. mRNA fold change of immune genes was profiled by RT2 Profiler PCR Array Gene Expression Analysis. Left panel indicated group of CoV-2-infected Caco-2-ACE2-N cells, shortly named with “Caco-2 ACE2-N”; data were presented with mRNA fold change relative to Mock-treated Caco-2-ACE2-N cells. Right panel indicated group of PBMCs cocultured with CoV-2-infected Caco-2-ACE2-N cells, shortly named with “PBMC”; data were presented with mRNA fold change relative to control PBMCs cocultured with Mock-treated Caco-2-ACE2-N cells. (B) CD69 expression profile on MAIT cells in various coculture groups. “Isotype ctr” indicated isotype antibody staining; “Mock” group indicated MAIT cells cocultured with Mock-treated IECs (Caco-2-ACE-2-N); “CoV-2” group indicated MAIT cells cocultured with CoV-2-infected IECs. (C) Representative flow cytometry dot plot of IFNγ expression in NK and MAIT cells after coculture with either Mock or CoV-2 infected IECs (Caco-2-ACE-2-N). (D) Inflammatory cytokine (IFNγ, GrzB, TNFα) expression in MAIT cells determined by FACS with positive population percentage. “Mock” indicated group of MAIT cells cocultured with Mock-treated IECs; “CoV-2” indicated group of MAIT cells cocultured with CoV-2-infected IECs (E) Depletion of IL18 by adding αIL18 Ab into medium at the beginning of coculture. Cytokine expression in MAIT with depletion of IL18 in coculture or with rIL18 addition alone in PBMC culture medium. All data are represented as mean ± SEM of three replicates. Statistical significance is indicated. (ns, not significant, **p<0.01 ***p<0.001, unpaired t test or One-way ANOVA).

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: SARS-CoV-2 infection of intestinal epithelia cells sensed by RIG-I and DHX-15 evokes innate immune response and immune cross-talk

    doi: 10.3389/fcimb.2022.1035711

    Figure Lengend Snippet: Cross-talk between CoV-2-infected epithelial cells and immune cells activated MAIT and exacerbated inflammation. (A) Heatmap of immunological profile of Caco-2-ACE2-N cells infected with CoV-2 and PBMC cocultured with CoV-2-infected Caco-2-ACE2-N cells. mRNA fold change of immune genes was profiled by RT2 Profiler PCR Array Gene Expression Analysis. Left panel indicated group of CoV-2-infected Caco-2-ACE2-N cells, shortly named with “Caco-2 ACE2-N”; data were presented with mRNA fold change relative to Mock-treated Caco-2-ACE2-N cells. Right panel indicated group of PBMCs cocultured with CoV-2-infected Caco-2-ACE2-N cells, shortly named with “PBMC”; data were presented with mRNA fold change relative to control PBMCs cocultured with Mock-treated Caco-2-ACE2-N cells. (B) CD69 expression profile on MAIT cells in various coculture groups. “Isotype ctr” indicated isotype antibody staining; “Mock” group indicated MAIT cells cocultured with Mock-treated IECs (Caco-2-ACE-2-N); “CoV-2” group indicated MAIT cells cocultured with CoV-2-infected IECs. (C) Representative flow cytometry dot plot of IFNγ expression in NK and MAIT cells after coculture with either Mock or CoV-2 infected IECs (Caco-2-ACE-2-N). (D) Inflammatory cytokine (IFNγ, GrzB, TNFα) expression in MAIT cells determined by FACS with positive population percentage. “Mock” indicated group of MAIT cells cocultured with Mock-treated IECs; “CoV-2” indicated group of MAIT cells cocultured with CoV-2-infected IECs (E) Depletion of IL18 by adding αIL18 Ab into medium at the beginning of coculture. Cytokine expression in MAIT with depletion of IL18 in coculture or with rIL18 addition alone in PBMC culture medium. All data are represented as mean ± SEM of three replicates. Statistical significance is indicated. (ns, not significant, **p<0.01 ***p<0.001, unpaired t test or One-way ANOVA).

    Article Snippet: After that, PBMC were harvested for immune genes profiling with customized RT2 Profiler PCR Array Gene Expression Kit (Qiagen).

    Techniques: Infection, Gene Expression, Control, Expressing, Staining, Flow Cytometry

    Quality of human ORF features on GMAP chip . (a) Intensity signals from the GMAP array (X-axis) plotted against signals from the Human Gene 1.0 ST array (Y-axis) following amplification of human ORFeome v5.1 pools and hybridization of the resulting probe to each of these arrays. Common features between the two arrays were used to calculate the Pearson correlation coefficient. (b) Distribution of signal intensities from replicate GMAP or Human 1.0 ST arrays as described in (a) for shared features. Hybridization behaviour for the huORF features compared to the (c) huGene features or the (d) 22-mer hairpin features for corresponding genes.

    Journal: BMC Genomics

    Article Title: A comprehensive platform for highly multiplexed mammalian functional genetic screens

    doi: 10.1186/1471-2164-12-213

    Figure Lengend Snippet: Quality of human ORF features on GMAP chip . (a) Intensity signals from the GMAP array (X-axis) plotted against signals from the Human Gene 1.0 ST array (Y-axis) following amplification of human ORFeome v5.1 pools and hybridization of the resulting probe to each of these arrays. Common features between the two arrays were used to calculate the Pearson correlation coefficient. (b) Distribution of signal intensities from replicate GMAP or Human 1.0 ST arrays as described in (a) for shared features. Hybridization behaviour for the huORF features compared to the (c) huGene features or the (d) 22-mer hairpin features for corresponding genes.

    Article Snippet: For comparative purposes, we also included up to three additional features found on the human expression profiling Affymetrix Gene 1.0 ST array for 18,088 of these sequences.

    Techniques: Amplification, Hybridization