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rt2 profiler pcr array gene expression for senescence-associated genes in 96-well plates  (Qiagen)


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    Qiagen rt2 profiler pcr array gene expression for senescence-associated genes in 96-well plates
    Rt2 Profiler Pcr Array Gene Expression For Senescence Associated Genes In 96 Well Plates, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/array+gene+expression+profiling/abi+7500/pmc09963163-170-16-24
    Average 90 stars, based on 1 article reviews
    rt2 profiler pcr array gene expression for senescence-associated genes in 96-well plates - by Bioz Stars, 2026-11
    90/100 stars

    Images

    Related Articles

    other:

    Article Title: Influence of Bio-Fertilizer Type and Amount Jointly on Microbial Community Composition, Crop Production and Soil Health
    Article Snippet: The ABI 7500 Real-Time PCR detection system (MoBio Laboratories, Carlsbad, CA, USA) was used to quantify the copy number of bacterial 16S rRNA gene.

    Article Title: Interleukin-33 Expression on Treatment Outcomes and Prognosis in Brazilian Breast Cancer Patients Undergoing Neoadjuvant Chemotherapy.
    Article Snippet: Quantitative PCR was performed at 7500 Fast Real-Time PCR® (Qiagen, USA) with the following setup: initial denaturation at 95 ◦C for 10 min, followed by 40 cycles of 95 ◦C for 15 s, 60 ◦C for 50 s, and 30 s at 72 ◦C.

    Article Title: Interleukin-33 Expression on Treatment Outcomes and Prognosis in Brazilian Breast Cancer Patients Undergoing Neoadjuvant Chemotherapy
    Article Snippet: Quantitative PCR was performed at 7500 Fast Real-Time PCR ® (Qiagen, USA) with the following setup: initial denaturation at 95 °C for 10 min, followed by 40 cycles of 95 °C for 15 s, 60 °C for 50 s, and 30 s at 72 °C.

    Gene Expression:

    Article Title: Neutralization of IL-33 modifies the type 2 and type 3 inflammatory signature of viral induced asthma exacerbation
    Article Snippet: The total product of this reaction was diluted in SYBR Green ROX qPCR master mix and nuclease-free, deionized and distilled water (Hyclone, Logan, UT) and distributed across a 96-well Mouse Allergy & Asthma RT 2 Profiler PCR array (Qiagen). .. The plate was analyzed on the ABI 7500 using a 25 uL reaction volume and cycling at 95.0 °C for 10 min, followed by 40 cycles at 95.0 °C for 15 s, 60.0 °C for 1 min. Ct values below 35 cycles were considered positive, and analysis for changes in gene expression was completed using the data analysis software available from Qiagen. ..

    Article Title: Heterogenous Differences in Cellular Senescent Phenotypes in Pre-Eclampsia and IUGR following Quantitative Assessment of Multiple Biomarkers of Senescence
    Article Snippet: Placental tissue was mechanically homogenised in Trizol Reagent (InvitrogenTM Thermo Fisher Scientific, Dublin, Ireland) using TissueLyser II (Qiagen, Manchester, UK) and RNA was isolated which was subsequently purified using the Qiagen RNeasy ® Plus Mini (#74134) clean-up protocol according to the manufacturer’s instructions. cDNA synthesis was performed using the Superscript IV Double-Stranded cDNA Synthesis Kit (InvitrogenTM Thermo Fisher Scientific, Ireland). .. Initial assessment for senescence-associated genes were performed using the RT2 Profiler PCR Array Gene Expression for senescence-associated genes in 96-well plates (Cat no. PAHS-050Z, Qiagen, Ireland). ..

    Article Title: Profiles of lipid, protein and microRNA expression in exosomes derived from intestinal epithelial cells after ischemia-reperfusion injury in a cellular hypoxia model
    Article Snippet: The extracted RNA was then reverse-transcribed to cDNA using the miScript II RT Kit (Qiagen). .. The gene expression profile of 84 miRNAs was examined using a qRT-PCR assay with the miScript miRNA PCR Array (Human miFinder in 96-well, Qiagen, Germany; 331221-MIHS-001ZD-2) according to the manufacturer’s instructions. ..

    Software:

    Article Title: Neutralization of IL-33 modifies the type 2 and type 3 inflammatory signature of viral induced asthma exacerbation
    Article Snippet: The total product of this reaction was diluted in SYBR Green ROX qPCR master mix and nuclease-free, deionized and distilled water (Hyclone, Logan, UT) and distributed across a 96-well Mouse Allergy & Asthma RT 2 Profiler PCR array (Qiagen). .. The plate was analyzed on the ABI 7500 using a 25 uL reaction volume and cycling at 95.0 °C for 10 min, followed by 40 cycles at 95.0 °C for 15 s, 60.0 °C for 1 min. Ct values below 35 cycles were considered positive, and analysis for changes in gene expression was completed using the data analysis software available from Qiagen. ..

    Polymerase Chain Reaction:

    Article Title: Heterogenous Differences in Cellular Senescent Phenotypes in Pre-Eclampsia and IUGR following Quantitative Assessment of Multiple Biomarkers of Senescence
    Article Snippet: Placental tissue was mechanically homogenised in Trizol Reagent (InvitrogenTM Thermo Fisher Scientific, Dublin, Ireland) using TissueLyser II (Qiagen, Manchester, UK) and RNA was isolated which was subsequently purified using the Qiagen RNeasy ® Plus Mini (#74134) clean-up protocol according to the manufacturer’s instructions. cDNA synthesis was performed using the Superscript IV Double-Stranded cDNA Synthesis Kit (InvitrogenTM Thermo Fisher Scientific, Ireland). .. Initial assessment for senescence-associated genes were performed using the RT2 Profiler PCR Array Gene Expression for senescence-associated genes in 96-well plates (Cat no. PAHS-050Z, Qiagen, Ireland). ..

    Article Title: Profiles of lipid, protein and microRNA expression in exosomes derived from intestinal epithelial cells after ischemia-reperfusion injury in a cellular hypoxia model
    Article Snippet: The extracted RNA was then reverse-transcribed to cDNA using the miScript II RT Kit (Qiagen). .. The gene expression profile of 84 miRNAs was examined using a qRT-PCR assay with the miScript miRNA PCR Array (Human miFinder in 96-well, Qiagen, Germany; 331221-MIHS-001ZD-2) according to the manufacturer’s instructions. ..

    Quantitative RT-PCR:

    Article Title: Profiles of lipid, protein and microRNA expression in exosomes derived from intestinal epithelial cells after ischemia-reperfusion injury in a cellular hypoxia model
    Article Snippet: The extracted RNA was then reverse-transcribed to cDNA using the miScript II RT Kit (Qiagen). .. The gene expression profile of 84 miRNAs was examined using a qRT-PCR assay with the miScript miRNA PCR Array (Human miFinder in 96-well, Qiagen, Germany; 331221-MIHS-001ZD-2) according to the manufacturer’s instructions. ..

    DNA Extraction:

    Article Title: Broad ecological threats of an invasive hornet revealed through a deep sequencing approach.
    Article Snippet: After incubation with proteinase K, the lysates were stored at room temperature (for no longer than 6 months, as advised by the manufacturer, Qiagen) until a suitable number were prepared to be purified with the 96 well-plate kit. .. At that time, the lysates were centrifuged to pellet undissolved particles, and 200 μl of clear supernatant was transferred to the Qiagen collection tubes for DNA extraction from tissue in 96 well-plate format, following the manufacturer’s protocol for extraction from tissue. ..

    Extraction:

    Article Title: Broad ecological threats of an invasive hornet revealed through a deep sequencing approach.
    Article Snippet: After incubation with proteinase K, the lysates were stored at room temperature (for no longer than 6 months, as advised by the manufacturer, Qiagen) until a suitable number were prepared to be purified with the 96 well-plate kit. .. At that time, the lysates were centrifuged to pellet undissolved particles, and 200 μl of clear supernatant was transferred to the Qiagen collection tubes for DNA extraction from tissue in 96 well-plate format, following the manufacturer’s protocol for extraction from tissue. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Treatment planning for immunotherapy based treatments using non-thermal ablation techniques
    Article Snippet: .. The resulting RNA samples went through 1st strand synthesis and qPCR amplification in an ABI 7500 FAST thermocycler, according to the Qiagen RT2 profiler protocol. .. A custom, canine specific, superarray developed and validated in our Lab was used, based on the Qiagen RT2 profiler platform.

    Amplification:

    Article Title: Treatment planning for immunotherapy based treatments using non-thermal ablation techniques
    Article Snippet: .. The resulting RNA samples went through 1st strand synthesis and qPCR amplification in an ABI 7500 FAST thermocycler, according to the Qiagen RT2 profiler protocol. .. A custom, canine specific, superarray developed and validated in our Lab was used, based on the Qiagen RT2 profiler platform.



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    Image Search Results


    Genes analyzed with the RT 2 Profiler PCR Array—Human DNA Damage Signaling Pathway kit (Qiagen, Germantown, MD, USA).

    Journal: Current Issues in Molecular Biology

    Article Title: DNA Double-Strand Break Repair Inhibitors: YU238259, A12B4C3 and DDRI-18 Overcome the Cisplatin Resistance in Human Ovarian Cancer Cells, but Not under Hypoxia Conditions

    doi: 10.3390/cimb45100500

    Figure Lengend Snippet: Genes analyzed with the RT 2 Profiler PCR Array—Human DNA Damage Signaling Pathway kit (Qiagen, Germantown, MD, USA).

    Article Snippet: Expression of genes related to cell response to DNA damage was carried out using Real-time PCR using the RT 2 Profiler PCR Array—Human DNA Damage Signaling Pathway gene expression evaluation kit (Qiagen, Germantown, MD, USA).

    Techniques:

    Cross-talk between CoV-2-infected epithelial cells and immune cells activated MAIT and exacerbated inflammation. (A) Heatmap of immunological profile of Caco-2-ACE2-N cells infected with CoV-2 and PBMC cocultured with CoV-2-infected Caco-2-ACE2-N cells. mRNA fold change of immune genes was profiled by RT2 Profiler PCR Array Gene Expression Analysis. Left panel indicated group of CoV-2-infected Caco-2-ACE2-N cells, shortly named with “Caco-2 ACE2-N”; data were presented with mRNA fold change relative to Mock-treated Caco-2-ACE2-N cells. Right panel indicated group of PBMCs cocultured with CoV-2-infected Caco-2-ACE2-N cells, shortly named with “PBMC”; data were presented with mRNA fold change relative to control PBMCs cocultured with Mock-treated Caco-2-ACE2-N cells. (B) CD69 expression profile on MAIT cells in various coculture groups. “Isotype ctr” indicated isotype antibody staining; “Mock” group indicated MAIT cells cocultured with Mock-treated IECs (Caco-2-ACE-2-N); “CoV-2” group indicated MAIT cells cocultured with CoV-2-infected IECs. (C) Representative flow cytometry dot plot of IFNγ expression in NK and MAIT cells after coculture with either Mock or CoV-2 infected IECs (Caco-2-ACE-2-N). (D) Inflammatory cytokine (IFNγ, GrzB, TNFα) expression in MAIT cells determined by FACS with positive population percentage. “Mock” indicated group of MAIT cells cocultured with Mock-treated IECs; “CoV-2” indicated group of MAIT cells cocultured with CoV-2-infected IECs (E) Depletion of IL18 by adding αIL18 Ab into medium at the beginning of coculture. Cytokine expression in MAIT with depletion of IL18 in coculture or with rIL18 addition alone in PBMC culture medium. All data are represented as mean ± SEM of three replicates. Statistical significance is indicated. (ns, not significant, **p<0.01 ***p<0.001, unpaired t test or One-way ANOVA).

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: SARS-CoV-2 infection of intestinal epithelia cells sensed by RIG-I and DHX-15 evokes innate immune response and immune cross-talk

    doi: 10.3389/fcimb.2022.1035711

    Figure Lengend Snippet: Cross-talk between CoV-2-infected epithelial cells and immune cells activated MAIT and exacerbated inflammation. (A) Heatmap of immunological profile of Caco-2-ACE2-N cells infected with CoV-2 and PBMC cocultured with CoV-2-infected Caco-2-ACE2-N cells. mRNA fold change of immune genes was profiled by RT2 Profiler PCR Array Gene Expression Analysis. Left panel indicated group of CoV-2-infected Caco-2-ACE2-N cells, shortly named with “Caco-2 ACE2-N”; data were presented with mRNA fold change relative to Mock-treated Caco-2-ACE2-N cells. Right panel indicated group of PBMCs cocultured with CoV-2-infected Caco-2-ACE2-N cells, shortly named with “PBMC”; data were presented with mRNA fold change relative to control PBMCs cocultured with Mock-treated Caco-2-ACE2-N cells. (B) CD69 expression profile on MAIT cells in various coculture groups. “Isotype ctr” indicated isotype antibody staining; “Mock” group indicated MAIT cells cocultured with Mock-treated IECs (Caco-2-ACE-2-N); “CoV-2” group indicated MAIT cells cocultured with CoV-2-infected IECs. (C) Representative flow cytometry dot plot of IFNγ expression in NK and MAIT cells after coculture with either Mock or CoV-2 infected IECs (Caco-2-ACE-2-N). (D) Inflammatory cytokine (IFNγ, GrzB, TNFα) expression in MAIT cells determined by FACS with positive population percentage. “Mock” indicated group of MAIT cells cocultured with Mock-treated IECs; “CoV-2” indicated group of MAIT cells cocultured with CoV-2-infected IECs (E) Depletion of IL18 by adding αIL18 Ab into medium at the beginning of coculture. Cytokine expression in MAIT with depletion of IL18 in coculture or with rIL18 addition alone in PBMC culture medium. All data are represented as mean ± SEM of three replicates. Statistical significance is indicated. (ns, not significant, **p<0.01 ***p<0.001, unpaired t test or One-way ANOVA).

    Article Snippet: After that, PBMC were harvested for immune genes profiling with customized RT2 Profiler PCR Array Gene Expression Kit (Qiagen).

    Techniques: Infection, Gene Expression, Control, Expressing, Staining, Flow Cytometry

    Quality of human ORF features on GMAP chip . (a) Intensity signals from the GMAP array (X-axis) plotted against signals from the Human Gene 1.0 ST array (Y-axis) following amplification of human ORFeome v5.1 pools and hybridization of the resulting probe to each of these arrays. Common features between the two arrays were used to calculate the Pearson correlation coefficient. (b) Distribution of signal intensities from replicate GMAP or Human 1.0 ST arrays as described in (a) for shared features. Hybridization behaviour for the huORF features compared to the (c) huGene features or the (d) 22-mer hairpin features for corresponding genes.

    Journal: BMC Genomics

    Article Title: A comprehensive platform for highly multiplexed mammalian functional genetic screens

    doi: 10.1186/1471-2164-12-213

    Figure Lengend Snippet: Quality of human ORF features on GMAP chip . (a) Intensity signals from the GMAP array (X-axis) plotted against signals from the Human Gene 1.0 ST array (Y-axis) following amplification of human ORFeome v5.1 pools and hybridization of the resulting probe to each of these arrays. Common features between the two arrays were used to calculate the Pearson correlation coefficient. (b) Distribution of signal intensities from replicate GMAP or Human 1.0 ST arrays as described in (a) for shared features. Hybridization behaviour for the huORF features compared to the (c) huGene features or the (d) 22-mer hairpin features for corresponding genes.

    Article Snippet: For comparative purposes, we also included up to three additional features found on the human expression profiling Affymetrix Gene 1.0 ST array for 18,088 of these sequences.

    Techniques: Amplification, Hybridization