primer antisense mix (New England Biolabs)
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Primer Antisense Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1645 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antisense+primer/Random+Primer+Mix/pm40062677-82-22-38
Average 97 stars, based on 1645 article reviews
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cDNA Synthesis:Article Title: Nuclear envelope budding enables export of large transcripts in muscle cells. Article Snippet: RNA was eluted in 50 μl of nuclease-free water and the concentration was determined using a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific). .. First-strand cDNA synthesis was performed on all RNA samples using the LunaScript® RT Master Mix Kit (NEB #E3025L) combined with the Article Title: Essential role of cytochrome bc 1 in Pseudomonas aeruginosa cell physiology and virulence Article Snippet: .. Supernatant total RNA was extracted using TRIzol LS (Thermo Fisher Scientific, Waltham, MA) and quantified on a Qubit 3.0 fluorometer using the AccuBlue Broad Range RNA Quantitation Kit (Biotium, Fremont, CA), followed by cDNA synthesis with the ProtoScript II First Strand cDNA Synthesis Kit using the Construct:Article Title: Metabolic and behavioral effects of neurofibromin result from differential recruitment of MAPK and mTOR signaling. Article Snippet: RNA was isolated with the Qiagen RNeasy Lipid Tissue Mini Kit. .. Complimentary DNA library was constructed using LunaScript RT Master Mix (New England Biolabs, catalog #E3025L) with the Article Title: Metabolic and behavioral effects of neurofibromin result from differential recruitment of MAPK and mTOR signaling Article Snippet: RNA was isolated with the Qiagen RNeasy Lipid Tissue Mini Kit. .. Complimentary DNA library was constructed using LunaScript RT Master Mix (New England Biolabs, catalog #E3025L) with the Quantitative RT-PCR:Article Title: Anti-PECAM-1 antibodies: another tool for visualization of Reissner´s fiber and the subcommissural organ in rat central nervous system. Article Snippet: .. The cDNA for the RT-qPCR was prepared by reverse transcription of the extracted total RNA and a mixture of random hexamer primers and oligoT primers ( Article Title: Anti-PECAM-1 antibodies: another tool for visualization of Reissner´s fiber and the subcommissural organ in rat central nervous system Article Snippet: .. The cDNA for the RT-qPCR was prepared by reverse transcription of the extracted total RNA and a mixture of random hexamer primers and oligoT primers ( Reverse Transcription:Article Title: Anti-PECAM-1 antibodies: another tool for visualization of Reissner´s fiber and the subcommissural organ in rat central nervous system. Article Snippet: .. The cDNA for the RT-qPCR was prepared by reverse transcription of the extracted total RNA and a mixture of random hexamer primers and oligoT primers ( Article Title: Anti-PECAM-1 antibodies: another tool for visualization of Reissner´s fiber and the subcommissural organ in rat central nervous system Article Snippet: .. The cDNA for the RT-qPCR was prepared by reverse transcription of the extracted total RNA and a mixture of random hexamer primers and oligoT primers ( Article Title: Comparative Analysis of Prenatal Stress Models: Placental and Neurodevelopmental Outcomes in Mice Article Snippet: .. E14 and E18 placenta and ventral forebrain from one male and one female per litter were randomly selected for RNA isolation (Trizol) and quantified and verified by Spectrophotometer (Nanodrop). cDNA was synthesized (New England Biolabs AMV Reverse Transcriptase; NEB M0227L, Random Hexamer:Article Title: Anti-PECAM-1 antibodies: another tool for visualization of Reissner´s fiber and the subcommissural organ in rat central nervous system. Article Snippet: .. The cDNA for the RT-qPCR was prepared by reverse transcription of the extracted total RNA and a mixture of random hexamer primers and oligoT primers ( Article Title: Anti-PECAM-1 antibodies: another tool for visualization of Reissner´s fiber and the subcommissural organ in rat central nervous system Article Snippet: .. The cDNA for the RT-qPCR was prepared by reverse transcription of the extracted total RNA and a mixture of random hexamer primers and oligoT primers ( Quantitation Assay:Article Title: Essential role of cytochrome bc 1 in Pseudomonas aeruginosa cell physiology and virulence Article Snippet: .. Supernatant total RNA was extracted using TRIzol LS (Thermo Fisher Scientific, Waltham, MA) and quantified on a Qubit 3.0 fluorometer using the AccuBlue Broad Range RNA Quantitation Kit (Biotium, Fremont, CA), followed by cDNA synthesis with the ProtoScript II First Strand cDNA Synthesis Kit using the Real-time Polymerase Chain Reaction:Article Title: Essential role of cytochrome bc 1 in Pseudomonas aeruginosa cell physiology and virulence Article Snippet: .. Supernatant total RNA was extracted using TRIzol LS (Thermo Fisher Scientific, Waltham, MA) and quantified on a Qubit 3.0 fluorometer using the AccuBlue Broad Range RNA Quantitation Kit (Biotium, Fremont, CA), followed by cDNA synthesis with the ProtoScript II First Strand cDNA Synthesis Kit using the Software:Article Title: Essential role of cytochrome bc 1 in Pseudomonas aeruginosa cell physiology and virulence Article Snippet: .. Supernatant total RNA was extracted using TRIzol LS (Thermo Fisher Scientific, Waltham, MA) and quantified on a Qubit 3.0 fluorometer using the AccuBlue Broad Range RNA Quantitation Kit (Biotium, Fremont, CA), followed by cDNA synthesis with the ProtoScript II First Strand cDNA Synthesis Kit using the Isolation:Article Title: Comparative Analysis of Prenatal Stress Models: Placental and Neurodevelopmental Outcomes in Mice Article Snippet: .. E14 and E18 placenta and ventral forebrain from one male and one female per litter were randomly selected for RNA isolation (Trizol) and quantified and verified by Spectrophotometer (Nanodrop). cDNA was synthesized (New England Biolabs AMV Reverse Transcriptase; NEB M0227L, Spectrophotometry:Article Title: Comparative Analysis of Prenatal Stress Models: Placental and Neurodevelopmental Outcomes in Mice Article Snippet: .. E14 and E18 placenta and ventral forebrain from one male and one female per litter were randomly selected for RNA isolation (Trizol) and quantified and verified by Spectrophotometer (Nanodrop). cDNA was synthesized (New England Biolabs AMV Reverse Transcriptase; NEB M0227L, Synthesized:Article Title: Comparative Analysis of Prenatal Stress Models: Placental and Neurodevelopmental Outcomes in Mice Article Snippet: .. E14 and E18 placenta and ventral forebrain from one male and one female per litter were randomly selected for RNA isolation (Trizol) and quantified and verified by Spectrophotometer (Nanodrop). cDNA was synthesized (New England Biolabs AMV Reverse Transcriptase; NEB M0227L, |
