nova2 vectors (OriGene)

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OriGene nova2 vectors

Fig. 3 MiR-7-5p directly targets <t>NOVA2</t> expression. a – The sequence of human miR-7-p and the predicted binding sites with miR-7-5p within the NOVA2 untranslated region (3′-UTR) are shown. b – MiR-7-5p treatment suppressed NOVA2 expression in A549 and SPCA-1 cells. The cells were cultured with or without miR-7-5p for 24 h, and then collected and used for western blotting assays to determine NOVA2 expression. β-actin was set as a loading control. The quantitative data from western blotting assays were measured with ImageJ software. Data are ratios of NOVA2 to β-actin. c – MiR-7-5p stimulation inhibited NOVA2 mRNA in A549 and SPCA-1 cells. A549 cells were co-transfected with luciferase plasmids containing the wild-type (WT) NOVA2 3′-UTR or mutant-type (Mut) NOVA2 3′-UTR. The cells were also treated with miR-7-5p at the same time. The cells were lysed to measure the relative luciferase activity. Quantitative data are presented as the means ± SEM, n = 3. ***p < 0.001 compared with the NC mimic group. d – NOVA2 expression in NSCLC tissues and adjacent non-tumor tissues was measured using qPCR. Quantitative data are presented as the means ± SEM. ***p < 0.001 compared with the NC mimic group. e – NOVA2 expression in a panel of human lung cell lines and human lung epithelial BEAS-2B cells. NOVA2 expression in BEAS-2B cells was set as 100%. Quantitative data are presented as the means ± SEM, n = 3. ***p < 0.001 compared with the BEAS- 2B group. f – Analysis of the correlation between miR-7-5p and NOVA2 expression in tumors. NOVA2 expression was inversely correlated with miR-7-5p expression in NSCLC tissues. The miR-206 mRNA level was set as the X axes, and the TFR1 mRNA level was set as the Y axes. R stands for goodness of fit. The p value stands for slope significance

Nova2 Vectors, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anova/NOVA2+(NM_002516)+Human+3'+UTR+Clone/pm31832068-57-3-8
Average 93 stars, based on 2 article reviews

nova2 vectors - by Bioz Stars, 2026-10

93/100 stars

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1) Product Images from "MiR-7-5p suppresses tumor metastasis of non-small cell lung cancer by targeting NOVA2."

Article Title: MiR-7-5p suppresses tumor metastasis of non-small cell lung cancer by targeting NOVA2.

Journal: Cellular & molecular biology letters

doi: 10.1186/s11658-019-0188-3

Fig. 3 MiR-7-5p directly targets NOVA2 expression. a – The sequence of human miR-7-p and the predicted binding sites with miR-7-5p within the NOVA2 untranslated region (3′-UTR) are shown. b – MiR-7-5p treatment suppressed NOVA2 expression in A549 and SPCA-1 cells. The cells were cultured with or without miR-7-5p for 24 h, and then collected and used for western blotting assays to determine NOVA2 expression. β-actin was set as a loading control. The quantitative data from western blotting assays were measured with ImageJ software. Data are ratios of NOVA2 to β-actin. c – MiR-7-5p stimulation inhibited NOVA2 mRNA in A549 and SPCA-1 cells. A549 cells were co-transfected with luciferase plasmids containing the wild-type (WT) NOVA2 3′-UTR or mutant-type (Mut) NOVA2 3′-UTR. The cells were also treated with miR-7-5p at the same time. The cells were lysed to measure the relative luciferase activity. Quantitative data are presented as the means ± SEM, n = 3. ***p < 0.001 compared with the NC mimic group. d – NOVA2 expression in NSCLC tissues and adjacent non-tumor tissues was measured using qPCR. Quantitative data are presented as the means ± SEM. ***p < 0.001 compared with the NC mimic group. e – NOVA2 expression in a panel of human lung cell lines and human lung epithelial BEAS-2B cells. NOVA2 expression in BEAS-2B cells was set as 100%. Quantitative data are presented as the means ± SEM, n = 3. ***p < 0.001 compared with the BEAS- 2B group. f – Analysis of the correlation between miR-7-5p and NOVA2 expression in tumors. NOVA2 expression was inversely correlated with miR-7-5p expression in NSCLC tissues. The miR-206 mRNA level was set as the X axes, and the TFR1 mRNA level was set as the Y axes. R stands for goodness of fit. The p value stands for slope significance

Figure Legend Snippet: Fig. 3 MiR-7-5p directly targets NOVA2 expression. a – The sequence of human miR-7-p and the predicted binding sites with miR-7-5p within the NOVA2 untranslated region (3′-UTR) are shown. b – MiR-7-5p treatment suppressed NOVA2 expression in A549 and SPCA-1 cells. The cells were cultured with or without miR-7-5p for 24 h, and then collected and used for western blotting assays to determine NOVA2 expression. β-actin was set as a loading control. The quantitative data from western blotting assays were measured with ImageJ software. Data are ratios of NOVA2 to β-actin. c – MiR-7-5p stimulation inhibited NOVA2 mRNA in A549 and SPCA-1 cells. A549 cells were co-transfected with luciferase plasmids containing the wild-type (WT) NOVA2 3′-UTR or mutant-type (Mut) NOVA2 3′-UTR. The cells were also treated with miR-7-5p at the same time. The cells were lysed to measure the relative luciferase activity. Quantitative data are presented as the means ± SEM, n = 3. ***p < 0.001 compared with the NC mimic group. d – NOVA2 expression in NSCLC tissues and adjacent non-tumor tissues was measured using qPCR. Quantitative data are presented as the means ± SEM. ***p < 0.001 compared with the NC mimic group. e – NOVA2 expression in a panel of human lung cell lines and human lung epithelial BEAS-2B cells. NOVA2 expression in BEAS-2B cells was set as 100%. Quantitative data are presented as the means ± SEM, n = 3. ***p < 0.001 compared with the BEAS- 2B group. f – Analysis of the correlation between miR-7-5p and NOVA2 expression in tumors. NOVA2 expression was inversely correlated with miR-7-5p expression in NSCLC tissues. The miR-206 mRNA level was set as the X axes, and the TFR1 mRNA level was set as the Y axes. R stands for goodness of fit. The p value stands for slope significance

Techniques Used: Expressing, Sequencing, Binding Assay, Cell Culture, Western Blot, Control, Software, Transfection, Luciferase, Mutagenesis, Activity Assay

Fig. 4 NOVA2 overexpression reverses the inhibitory effect of miR-7-5p on A549 and SPCA-1 cells. a – NOVA2 overexpression partly decreased the miR-7-5p-mediated inhibitory effect on the proliferation of A549 and SPCA-1 cells. b and c – NOVA2 expression weakened the miR-7-5p-induced inhibitory effect on the migration of A549 and SPCA-1 cells. A549 and SPCA-1 cells were transfected with NOVA2 vector. After 24 h, the transfected cells were used for wound-healing assays. Representative images and quantitative data are shown in B and C, respectively. d and e – NOVA2 overexpression attenuated the miR-7-5p-mediated effect on the invasion of A549 and SPCA-1 cells. Representative images and quantitative data are shown in D and E, respectively. Quantitative data are presented as the means ± SEM. ***p < 0.001 compared with NC vector group. f – NOVA2 overexpression blocked miR-7-5p-mediated downregulation of EMT markers. A549 and SPCA-1 cells were transfected with NOVA2 vector or NC vector and then treated with miR-7-5p for 24 h. After that, the cells were collected and used for western blotting assays. The quantitative data from western blotting assays were measured with ImageJ software. Data are ratios of respective EMT marker to β-actin

Figure Legend Snippet: Fig. 4 NOVA2 overexpression reverses the inhibitory effect of miR-7-5p on A549 and SPCA-1 cells. a – NOVA2 overexpression partly decreased the miR-7-5p-mediated inhibitory effect on the proliferation of A549 and SPCA-1 cells. b and c – NOVA2 expression weakened the miR-7-5p-induced inhibitory effect on the migration of A549 and SPCA-1 cells. A549 and SPCA-1 cells were transfected with NOVA2 vector. After 24 h, the transfected cells were used for wound-healing assays. Representative images and quantitative data are shown in B and C, respectively. d and e – NOVA2 overexpression attenuated the miR-7-5p-mediated effect on the invasion of A549 and SPCA-1 cells. Representative images and quantitative data are shown in D and E, respectively. Quantitative data are presented as the means ± SEM. ***p < 0.001 compared with NC vector group. f – NOVA2 overexpression blocked miR-7-5p-mediated downregulation of EMT markers. A549 and SPCA-1 cells were transfected with NOVA2 vector or NC vector and then treated with miR-7-5p for 24 h. After that, the cells were collected and used for western blotting assays. The quantitative data from western blotting assays were measured with ImageJ software. Data are ratios of respective EMT marker to β-actin

Techniques Used: Over Expression, Expressing, Migration, Transfection, Plasmid Preparation, Western Blot, Software, Marker

Expressing:

Article Title: Resina fotopolimerizable para impresora 3D, resinas a base de PMMA de termocurado, cámara de post-polimerización, rugosidad superficial, pulido
Article Snippet: Varianza Mínimo Máximo GRUPO A (1) 10 0,1629 0,0379 0,0014 0,1000 0,2240 GRUPO B (2) 10 0,1086 0,0296 0,0009 0,0775 0,1610 Reporte de medias VAR Media 95% LCL 95% UCL GRUPO A (1) 0,1629 0,1358 0,1900 GRUPO B (2) 0,1086 0,0874 0,1297 Diferencia de medias (1-2) 0,0544 0,0224 0,0863 Prueba t suponiendo varianzas iguales Diferencia de Medias Hipotetizada 0,0000 Diferencia de medias 0,0544 Varianza Combinada 0,0012 Estadístico de la prueba 3,5758 Grados de Libertad 18 Test de Student t Valor Crítico (5%) -1,7341 Valor p 0,9989 H1 (5%) rechazado ANOVA Origen de la Variación d.f.

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nova2 vectors (OriGene)

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1) Product Images from "MiR-7-5p suppresses tumor metastasis of non-small cell lung cancer by targeting NOVA2."

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Expressing:

Sequencing:

Binding Assay:

Cell Culture:

Western Blot:

Control:

Software:

Transfection:

Luciferase:

Mutagenesis:

Activity Assay:

Over Expression:

Migration:

Plasmid Preparation:

Marker:

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