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airyscan joint deconvolution processing module  (Carl Zeiss)


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    Structured Review

    Carl Zeiss airyscan joint deconvolution processing module
    ( A , B ) HUVEC were treated with histamine (100 µM, Sigma-Aldrich) or thrombin (1 U/ml, CalBiochem) for 10 min, or left untreated with equivalent volumes of vehicle (media) as controls. Cells were subsequently fixed, permeabilized and stained for plakoglobin, β-catenin, and VE-cadherin, all visualized in grayscale to ensure unbiased comparative analysis. Higher magnification images (63X) were acquired using a Zeiss LSM 980 confocal microscope equipped with an <t>Airyscan</t> detector ( A ), while lower magnification images (40X) were captured on a Zeiss LSM 880 confocal microscope ( B ). Scale bars, 25 µm.
    Airyscan Joint Deconvolution Processing Module, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 94/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/airyscan+joint+deconvolution+processing+module/ZEN+Module+Deconvolution/pmc13043941-504-8-17
    Average 94 stars, based on 35 article reviews
    airyscan joint deconvolution processing module - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Plakoglobin transmits tension across VE-cadherin for vascular leak formation and leukocyte diapedesis"

    Article Title: Plakoglobin transmits tension across VE-cadherin for vascular leak formation and leukocyte diapedesis

    Journal: The EMBO Journal

    doi: 10.1038/s44318-026-00732-0

    ( A , B ) HUVEC were treated with histamine (100 µM, Sigma-Aldrich) or thrombin (1 U/ml, CalBiochem) for 10 min, or left untreated with equivalent volumes of vehicle (media) as controls. Cells were subsequently fixed, permeabilized and stained for plakoglobin, β-catenin, and VE-cadherin, all visualized in grayscale to ensure unbiased comparative analysis. Higher magnification images (63X) were acquired using a Zeiss LSM 980 confocal microscope equipped with an Airyscan detector ( A ), while lower magnification images (40X) were captured on a Zeiss LSM 880 confocal microscope ( B ). Scale bars, 25 µm.
    Figure Legend Snippet: ( A , B ) HUVEC were treated with histamine (100 µM, Sigma-Aldrich) or thrombin (1 U/ml, CalBiochem) for 10 min, or left untreated with equivalent volumes of vehicle (media) as controls. Cells were subsequently fixed, permeabilized and stained for plakoglobin, β-catenin, and VE-cadherin, all visualized in grayscale to ensure unbiased comparative analysis. Higher magnification images (63X) were acquired using a Zeiss LSM 980 confocal microscope equipped with an Airyscan detector ( A ), while lower magnification images (40X) were captured on a Zeiss LSM 880 confocal microscope ( B ). Scale bars, 25 µm.

    Techniques Used: Staining, Microscopy

    Related Articles

    Microscopy:

    Article Title: Dynamic Mitotic Localization of the Centrosomal Kinases CDK1, Plk, AurK, and Nek2 in Dictyostelium amoebae
    Article Snippet: .. Image acquisition and microscopy were performed at a Zeiss AxioObserver system equipped with a Zeiss Axiocam 506 mono, a PlanApo 1.4/100× lens, and ZEN 2012 Software (blue edition), including the iterative deconvolution module (Carl Zeiss Mikroskopie GmbH, Jena, Germany). ..

    Software:

    Article Title: Dynamic Mitotic Localization of the Centrosomal Kinases CDK1, Plk, AurK, and Nek2 in Dictyostelium amoebae
    Article Snippet: .. Image acquisition and microscopy were performed at a Zeiss AxioObserver system equipped with a Zeiss Axiocam 506 mono, a PlanApo 1.4/100× lens, and ZEN 2012 Software (blue edition), including the iterative deconvolution module (Carl Zeiss Mikroskopie GmbH, Jena, Germany). ..

    Article Title: Defining the minimal structural requirements of DivIVA in filamentous Actinomycetota
    Article Snippet: To enable image deconvolution, point-spread functions (PSF) were determined using beads with excitation/emission wavelengths of 505/515 nm (green) and 633/660 nm (deep red) from the PS-Speck TM Microscope Point Source Kit (Invitrogen). .. The beads were imaged and PSFs calculated using the Deconvolution module in Zen software (Zeiss, ver 3.9). ..

    Article Title: Essential and dispensable domains of DivIVA for walled growth in filamentous Actinomycetota
    Article Snippet: To enable image deconvolution, point-spread functions (PSF) were determined using beads with excitation/emission wavelengths of 505/515 nm (green) and 633/660 nm (deep red) from the PS-Speck Microscope Point Source Kit (Invitrogen). .. The beads were imaged and PSFs calculated using the Deconvolution module in Zen software (Zeiss, ver 3.9). ..

    Article Title: Essential and dispensable domains of DivIVA for walled growth in filamentous Actinomycetota.
    Article Snippet: To enable image deconvolution, point-spread functions (PSF) were determined using beads with excitation/emission wavelengths of 505/515 nm (green) and 633/660 nm (deep red) from the PS-Speck Microscope Point Source Kit (Invitrogen). .. The beads were imaged and PSFs calculated using the Deconvolution module in Zen software (Zeiss, ver 3.9). ..

    Article Title: Plakoglobin transmits tension across VE-cadherin for vascular leak formation and leukocyte diapedesis.
    Article Snippet: .. Zeiss LSM 980 Airyscan acquisitions were processed with Airyscan Joint Deconvolution processing module in ZEN blue software (Zeiss) to improve signal-to-noise and spatial resolution; LSM 880 images were used without further deconvolution. ..

    Article Title: Dynamic regulation of autophagy during Semliki Forest virus infection of neuroblastoma cells
    Article Snippet: The secondary antibody, anti-rabbit AlexaFluor488, was diluted with blocking buffer and incubated at room temperature for 40 min. Coverslips were mounted using FluoroshieldTM with a DAPI mounting medium (Sigma-Aldrich, UK). .. Imaging of multiple z -axis positions was performed using a Zeiss AxioImager M2 system, and images were deconvoluted using the Zeiss Zen software deconvolution module. .. For quantification of LC3-positive punctae, images of only the DyLight 550 channel were selected at random and spots were counted in ImageJ.

    Article Title: Dynamic regulation of autophagy during Semliki Forest virus infection of neuroblastoma cells.
    Article Snippet: The secondary antibody, anti- rabbit AlexaFluor488, was diluted with blocking buffer and incubated at room temperature for 40 min. Coverslips were mounted using FluoroshieldTM with a DAPI mounting medium (Sigma- Aldrich, UK). .. Imaging of multiple z- axis positions was performed using a Zeiss AxioImager M2 system, and images were deconvoluted using the Zeiss Zen software deconvolution module. ..

    Article Title: Plakoglobin transmits tension across VE-cadherin for vascular leak formation and leukocyte diapedesis
    Article Snippet: .. Zeiss LSM 980 Airyscan acquisitions were processed with Airyscan Joint Deconvolution processing module in ZEN blue software (Zeiss) to improve signal-to-noise and spatial resolution; LSM 880 images were used without further deconvolution. ..

    Imaging:

    Article Title: Dynamic regulation of autophagy during Semliki Forest virus infection of neuroblastoma cells
    Article Snippet: The secondary antibody, anti-rabbit AlexaFluor488, was diluted with blocking buffer and incubated at room temperature for 40 min. Coverslips were mounted using FluoroshieldTM with a DAPI mounting medium (Sigma-Aldrich, UK). .. Imaging of multiple z -axis positions was performed using a Zeiss AxioImager M2 system, and images were deconvoluted using the Zeiss Zen software deconvolution module. .. For quantification of LC3-positive punctae, images of only the DyLight 550 channel were selected at random and spots were counted in ImageJ.

    Article Title: Dynamic regulation of autophagy during Semliki Forest virus infection of neuroblastoma cells.
    Article Snippet: The secondary antibody, anti- rabbit AlexaFluor488, was diluted with blocking buffer and incubated at room temperature for 40 min. Coverslips were mounted using FluoroshieldTM with a DAPI mounting medium (Sigma- Aldrich, UK). .. Imaging of multiple z- axis positions was performed using a Zeiss AxioImager M2 system, and images were deconvoluted using the Zeiss Zen software deconvolution module. ..



    Similar Products

    airyscan joint deconvolution processing module  (Carl Zeiss)
    94
    Carl Zeiss airyscan joint deconvolution processing module
    ( A , B ) HUVEC were treated with histamine (100 µM, Sigma-Aldrich) or thrombin (1 U/ml, CalBiochem) for 10 min, or left untreated with equivalent volumes of vehicle (media) as controls. Cells were subsequently fixed, permeabilized and stained for plakoglobin, β-catenin, and VE-cadherin, all visualized in grayscale to ensure unbiased comparative analysis. Higher magnification images (63X) were acquired using a Zeiss LSM 980 confocal microscope equipped with an <t>Airyscan</t> detector ( A ), while lower magnification images (40X) were captured on a Zeiss LSM 880 confocal microscope ( B ). Scale bars, 25 µm.
    Airyscan Joint Deconvolution Processing Module, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/airyscan+joint+deconvolution+processing+module/ZEN+Module+Deconvolution/pmc13043941-504-8-17
    Average 94 stars, based on 1 article reviews
    airyscan joint deconvolution processing module - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A , B ) HUVEC were treated with histamine (100 µM, Sigma-Aldrich) or thrombin (1 U/ml, CalBiochem) for 10 min, or left untreated with equivalent volumes of vehicle (media) as controls. Cells were subsequently fixed, permeabilized and stained for plakoglobin, β-catenin, and VE-cadherin, all visualized in grayscale to ensure unbiased comparative analysis. Higher magnification images (63X) were acquired using a Zeiss LSM 980 confocal microscope equipped with an Airyscan detector ( A ), while lower magnification images (40X) were captured on a Zeiss LSM 880 confocal microscope ( B ). Scale bars, 25 µm.

    Journal: The EMBO Journal

    Article Title: Plakoglobin transmits tension across VE-cadherin for vascular leak formation and leukocyte diapedesis

    doi: 10.1038/s44318-026-00732-0

    Figure Lengend Snippet: ( A , B ) HUVEC were treated with histamine (100 µM, Sigma-Aldrich) or thrombin (1 U/ml, CalBiochem) for 10 min, or left untreated with equivalent volumes of vehicle (media) as controls. Cells were subsequently fixed, permeabilized and stained for plakoglobin, β-catenin, and VE-cadherin, all visualized in grayscale to ensure unbiased comparative analysis. Higher magnification images (63X) were acquired using a Zeiss LSM 980 confocal microscope equipped with an Airyscan detector ( A ), while lower magnification images (40X) were captured on a Zeiss LSM 880 confocal microscope ( B ). Scale bars, 25 µm.

    Article Snippet: Zeiss LSM 980 Airyscan acquisitions were processed with Airyscan Joint Deconvolution processing module in ZEN blue software (Zeiss) to improve signal-to-noise and spatial resolution; LSM 880 images were used without further deconvolution.

    Techniques: Staining, Microscopy