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Clinical and Laboratory Standards Institute agar well-diffusion method
Agar Well Diffusion Method, supplied by Clinical and Laboratory Standards Institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/agar-well+diffusion+method/agar+well+diffusion+method/pmc11983983-71-18-26
Average 90 stars, based on 1 article reviews
agar well-diffusion method - by Bioz Stars, 2026-10
90/100 stars

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Diffusion-based Assay:

Article Title: Evaluation of Antibacterial Activity of Trichilia dregeana Sond. (Meliaceae) Methanolic Extract Against Gram-Positive and Gram-Negative Bacteria.
Article Snippet: .. The antibacterial activities of the crude extract of Trichilia dregeana Sond. were evaluated using the agar well diffusion method (Clinical and Laboratory Standards Institute 2018). ..

Article Title: Evaluation of Antibacterial Activity of Trichilia dregeana Sond. (Meliaceae) Methanolic Extract Against Gram‐Positive and Gram‐Negative Bacteria
Article Snippet: .. The antibacterial activities of the crude extract of Trichilia dregeana Sond. were evaluated using the agar well diffusion method (Clinical and Laboratory Standards Institute ). ..

Article Title: Removal of tetracycline antibiotic activity in water by stable cubic phase barium stannate-perovskite nanoparticles under energy-efficient blue light LED irradiation
Article Snippet: .. Antibacterial activity of TC (10 mg L −1 ) before and after treatment by BSO/LED system was assessed by the agar well diffusion method (Clinical and Laboratory Standards Institute, CLSI) as per standard protocol. ..

Article Title: Comprehensive assessment of the antidiarrheal properties of polyphenols from Psidium cattleianum Sabine's aerial parts.
Article Snippet: Ethnopharmacological relevance: Diarrhea is associated with high morbidity and mortality rates in developing countries and is ranked the second leading cause of infant mortality worldwide.. Psidium cattleianum Sabine leaves have been traditionally used as an anti-diarrheal remedy, however, no scientific study has thoroughly investigated its anti-diarrheal potential.. Aim of the study: To unravel the polyphenolic profile and explore the antidiarrheal potential of the defatted aqueous methanol extract (DAME) of Psidium cattleianum Sabine aerial parts through in vivo and in vitro experiments, alongside an inclusive in-silico analysis.

Article Title: Exploring antimicrobial properties and oral health benefits of salix subserrata willd: unveiling the potential beyond its use as traditional chewing stick.
Article Snippet: .. Antibacterial agar well diffusion assay The antibacterial activity of the crude extract was primarily evaluated qualitatively using an agar well diffusion method at various test doses (mg/mL) based on available plant mass, The Clinical and Laboratory Standards Institute guideline (CLSI M100) [52] was followed with minor adjustments to fit the current experimental setup. ..

Article Title: Novel DNA gyrase mutations in levofloxacin-resistant Helicobacter pylori isolates from southern Vietnam.
Article Snippet: .. H. pylori ATCC 43504 was used as a control strain, with an MIC of 0.064 μg/mL for levofloxacin.23 Quality control for antibiotic susceptibility testing was conducted in accordance with Clinical and Laboratory Standards Institute (CLSI) M45 guidelines, employing both agar diffusion and broth dilution methods.21,22 ..

other:

Article Title: Anti-biofilm and anti-quorum sensing activities of galloylquinic acid against clinical isolates of multidrug-resistant Pseudomonas aeruginosa in open wound infection: in vitro and in vivo efficacy studies.
Article Snippet: Preliminary evaluation of the antibacterial activity of GQAs against all test P. aeruginosa isolates was performed using the agar well-diffusion method as previously clarified by the Clinical and Laboratory Standards Institute [23].

Article Title: Anti-biofilm and anti-quorum sensing activities of galloylquinic acid against clinical isolates of multidrug-resistant Pseudomonas aeruginosa in open wound infection: in vitro and in vivo efficacy studies
Article Snippet: Preliminary evaluation of the antibacterial activity of GQAs against all test P. aeruginosa isolates was performed using the agar well-diffusion method as previously clarified by the Clinical and Laboratory Standards Institute [ ].

Activity Assay:

Article Title: Removal of tetracycline antibiotic activity in water by stable cubic phase barium stannate-perovskite nanoparticles under energy-efficient blue light LED irradiation
Article Snippet: .. Antibacterial activity of TC (10 mg L −1 ) before and after treatment by BSO/LED system was assessed by the agar well diffusion method (Clinical and Laboratory Standards Institute, CLSI) as per standard protocol. ..

Article Title: Exploring antimicrobial properties and oral health benefits of salix subserrata willd: unveiling the potential beyond its use as traditional chewing stick.
Article Snippet: .. Antibacterial agar well diffusion assay The antibacterial activity of the crude extract was primarily evaluated qualitatively using an agar well diffusion method at various test doses (mg/mL) based on available plant mass, The Clinical and Laboratory Standards Institute guideline (CLSI M100) [52] was followed with minor adjustments to fit the current experimental setup. ..

Control:

Article Title: Novel DNA gyrase mutations in levofloxacin-resistant Helicobacter pylori isolates from southern Vietnam.
Article Snippet: .. H. pylori ATCC 43504 was used as a control strain, with an MIC of 0.064 μg/mL for levofloxacin.23 Quality control for antibiotic susceptibility testing was conducted in accordance with Clinical and Laboratory Standards Institute (CLSI) M45 guidelines, employing both agar diffusion and broth dilution methods.21,22 ..



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Image Search Results


Journal: ACS Omega

Article Title: Synthesis and Characterization of Luminescent and Antibacterial Europium-Titanate nanotubes

doi: 10.1021/acsomega.5c08930

Figure Lengend Snippet: Antibacterial Activity of the Nanomaterials against S. aureus ATCC 25923 and E. coli ATCC 25922

Article Snippet: The antibacterial properties of TiNts, TiNts/Eu, tta, phen, [Eu (tta) 3 phen], and TiNts [Eu (tta) 3 phen] was first evaluated by the agar well diffusion method against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923.

Techniques: Activity Assay, Concentration Assay, Control

Antibacterial activity of TiNts, [Eu­(tta) 3 phen], TiNtsTi/Eu and TiNts­[Eu­(tta) 3 phen] against S. aureus ATCC 25923 and E. coli ATCC 25922. Note: 1 (3 mg mL –1 ), 2 (6 mg mL –1 ), 3 (12 mg mL –1 ), 4 (25 mg mL –1 ). B, corresponds to the negative control (sterilized deionized water); C, corresponds to the gentamicin (10 μg). All tests were performed in triplicate for each bacterium.

Journal: ACS Omega

Article Title: Synthesis and Characterization of Luminescent and Antibacterial Europium-Titanate nanotubes

doi: 10.1021/acsomega.5c08930

Figure Lengend Snippet: Antibacterial activity of TiNts, [Eu­(tta) 3 phen], TiNtsTi/Eu and TiNts­[Eu­(tta) 3 phen] against S. aureus ATCC 25923 and E. coli ATCC 25922. Note: 1 (3 mg mL –1 ), 2 (6 mg mL –1 ), 3 (12 mg mL –1 ), 4 (25 mg mL –1 ). B, corresponds to the negative control (sterilized deionized water); C, corresponds to the gentamicin (10 μg). All tests were performed in triplicate for each bacterium.

Article Snippet: The antibacterial properties of TiNts, TiNts/Eu, tta, phen, [Eu (tta) 3 phen], and TiNts [Eu (tta) 3 phen] was first evaluated by the agar well diffusion method against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923.

Techniques: Activity Assay, Negative Control

Antibacterial activity of TiNts­[Eu­(tta) 3 phen] against S. aureus ATCC 25923 and E. coli ATCC 25922. Note: 1 (6 mg mL –1 ), 2 (12 mg mL –1 ), 3 (25 mg mL –1 ), 4 (50 mg mL –1 ), 5 (100 mg mL –1 ).

Journal: ACS Omega

Article Title: Synthesis and Characterization of Luminescent and Antibacterial Europium-Titanate nanotubes

doi: 10.1021/acsomega.5c08930

Figure Lengend Snippet: Antibacterial activity of TiNts­[Eu­(tta) 3 phen] against S. aureus ATCC 25923 and E. coli ATCC 25922. Note: 1 (6 mg mL –1 ), 2 (12 mg mL –1 ), 3 (25 mg mL –1 ), 4 (50 mg mL –1 ), 5 (100 mg mL –1 ).

Article Snippet: The antibacterial properties of TiNts, TiNts/Eu, tta, phen, [Eu (tta) 3 phen], and TiNts [Eu (tta) 3 phen] was first evaluated by the agar well diffusion method against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923.

Techniques: Activity Assay