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multiplex fluidigm array  (fluidigm)


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    fluidigm multiplex fluidigm array
    Multiplex Fluidigm Array, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/access+array+target+specific+primers/Access+Array+Multiplex+Target-Specific+Primers/pm29404863-108-3-4
    Average 93 stars, based on 60 article reviews
    multiplex fluidigm array - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Sequencing:

    Article Title: PrimerPooler: automated primer pooling to prepare library for targeted sequencing
    Article Snippet: .. Figure 6: Coverage of targeted sequence regions by Fluidigm Access Array multiplex PCR and Illumina MiSeQ sequencing. .. Two FFPE DNA samples were tested under various experimental conditions including 50- and 75-ng DNA for pre-amplication, and 1l of 2.5- and 5.0-fold diluted pre-amplified products for Fluidigm Access Array multiplex PCR.

    Article Title: A New Threat to Limber Pine (<i>Pinus flexilis</i>) Restoration in Alberta and Beyond: First Documentation of a <i>Cronartium ribicola</i> race (<i>vcr4</i>) Virulent to <i>Cr4</i>-Controlled Major Gene Resistance
    Article Snippet: The coevolution of virulence reduces the effectiveness of host resistance to pathogens, posing a direct threat to forest species and their key ecosystem functions.. This is a threat to limber pine (Pinus flexilis), an endangered species in Canada due to rapid decline mainly driven by white pine blister rust caused by Cronartium ribicola.. We present the first report of a new, virulent race of C. ribicola (designated vcr4) that overcomes limber pine major gene (Cr4) resistance (MGR).

    Multiplex Assay:

    Article Title: PrimerPooler: automated primer pooling to prepare library for targeted sequencing
    Article Snippet: .. Figure 6: Coverage of targeted sequence regions by Fluidigm Access Array multiplex PCR and Illumina MiSeQ sequencing. .. Two FFPE DNA samples were tested under various experimental conditions including 50- and 75-ng DNA for pre-amplication, and 1l of 2.5- and 5.0-fold diluted pre-amplified products for Fluidigm Access Array multiplex PCR.

    Article Title: A New Threat to Limber Pine (<i>Pinus flexilis</i>) Restoration in Alberta and Beyond: First Documentation of a <i>Cronartium ribicola</i> race (<i>vcr4</i>) Virulent to <i>Cr4</i>-Controlled Major Gene Resistance
    Article Snippet: The coevolution of virulence reduces the effectiveness of host resistance to pathogens, posing a direct threat to forest species and their key ecosystem functions.. This is a threat to limber pine (Pinus flexilis), an endangered species in Canada due to rapid decline mainly driven by white pine blister rust caused by Cronartium ribicola.. We present the first report of a new, virulent race of C. ribicola (designated vcr4) that overcomes limber pine major gene (Cr4) resistance (MGR).

    Article Title: PrimerPooler: automated primer pooling to prepare library for targeted sequencing
    Article Snippet: Figure 6: Coverage of targeted sequence regions by Fluidigm Access Array multiplex PCR and Illumina MiSeQ sequencing. .. Two FFPE DNA samples were tested under various experimental conditions including 50- and 75-ng DNA for pre-amplication, and 1l of 2.5- and 5.0-fold diluted pre-amplified products for Fluidigm Access Array multiplex PCR. ..

    Article Title: PrimerPooler: automated primer pooling to prepare library for targeted sequencing
    Article Snippet: .. Based on the two DNA samples tested, both 50ng and 75ng starting amounts of DNA yielded very similar results, and similarly there was no obvious benefit when less diluted pre-amplified product (2.5 versus 5-fold dilution) was used for Fluidigm Access Array multiplex PCR ( ). ..

    Article Title: In vitro evidence that platelet-rich plasma stimulates cellular processes involved in endometrial regeneration.
    Article Snippet: For quantitative RT-PCR analysis, 1 μg of RNA was converted to complementary DNA (cDNA) using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, USA). .. Quantitative RT-PCR with multiplex Fluidigm array All cDNA samples from cultured eSF, eMSC, BM-MSC, and Ishikawa cells in control, aPRP, naPRP, aPPP, and naPPP groups (n = 3 in each group, in duplicate) were assayed by q-RT-PCR using the Fluidigm Dynamic Array Integrated Fluidic Circuits and the BioMark HD system (www.fluidigm.com/biomarksystem.html) as previously described [40, 49]. ..

    Polymerase Chain Reaction:

    Article Title: PrimerPooler: automated primer pooling to prepare library for targeted sequencing
    Article Snippet: .. Figure 6: Coverage of targeted sequence regions by Fluidigm Access Array multiplex PCR and Illumina MiSeQ sequencing. .. Two FFPE DNA samples were tested under various experimental conditions including 50- and 75-ng DNA for pre-amplication, and 1l of 2.5- and 5.0-fold diluted pre-amplified products for Fluidigm Access Array multiplex PCR.

    Article Title: A New Threat to Limber Pine (<i>Pinus flexilis</i>) Restoration in Alberta and Beyond: First Documentation of a <i>Cronartium ribicola</i> race (<i>vcr4</i>) Virulent to <i>Cr4</i>-Controlled Major Gene Resistance
    Article Snippet: The coevolution of virulence reduces the effectiveness of host resistance to pathogens, posing a direct threat to forest species and their key ecosystem functions.. This is a threat to limber pine (Pinus flexilis), an endangered species in Canada due to rapid decline mainly driven by white pine blister rust caused by Cronartium ribicola.. We present the first report of a new, virulent race of C. ribicola (designated vcr4) that overcomes limber pine major gene (Cr4) resistance (MGR).

    Article Title: PrimerPooler: automated primer pooling to prepare library for targeted sequencing
    Article Snippet: Figure 6: Coverage of targeted sequence regions by Fluidigm Access Array multiplex PCR and Illumina MiSeQ sequencing. .. Two FFPE DNA samples were tested under various experimental conditions including 50- and 75-ng DNA for pre-amplication, and 1l of 2.5- and 5.0-fold diluted pre-amplified products for Fluidigm Access Array multiplex PCR. ..

    Article Title: PrimerPooler: automated primer pooling to prepare library for targeted sequencing
    Article Snippet: .. Based on the two DNA samples tested, both 50ng and 75ng starting amounts of DNA yielded very similar results, and similarly there was no obvious benefit when less diluted pre-amplified product (2.5 versus 5-fold dilution) was used for Fluidigm Access Array multiplex PCR ( ). ..

    Amplification:

    Article Title: A New Threat to Limber Pine (<i>Pinus flexilis</i>) Restoration in Alberta and Beyond: First Documentation of a <i>Cronartium ribicola</i> race (<i>vcr4</i>) Virulent to <i>Cr4</i>-Controlled Major Gene Resistance
    Article Snippet: The coevolution of virulence reduces the effectiveness of host resistance to pathogens, posing a direct threat to forest species and their key ecosystem functions.. This is a threat to limber pine (Pinus flexilis), an endangered species in Canada due to rapid decline mainly driven by white pine blister rust caused by Cronartium ribicola.. We present the first report of a new, virulent race of C. ribicola (designated vcr4) that overcomes limber pine major gene (Cr4) resistance (MGR).

    Formalin-fixed Paraffin-Embedded:

    Article Title: PrimerPooler: automated primer pooling to prepare library for targeted sequencing
    Article Snippet: Figure 6: Coverage of targeted sequence regions by Fluidigm Access Array multiplex PCR and Illumina MiSeQ sequencing. .. Two FFPE DNA samples were tested under various experimental conditions including 50- and 75-ng DNA for pre-amplication, and 1l of 2.5- and 5.0-fold diluted pre-amplified products for Fluidigm Access Array multiplex PCR. ..

    Quantitative RT-PCR:

    Article Title: In vitro evidence that platelet-rich plasma stimulates cellular processes involved in endometrial regeneration.
    Article Snippet: For quantitative RT-PCR analysis, 1 μg of RNA was converted to complementary DNA (cDNA) using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, USA). .. Quantitative RT-PCR with multiplex Fluidigm array All cDNA samples from cultured eSF, eMSC, BM-MSC, and Ishikawa cells in control, aPRP, naPRP, aPPP, and naPPP groups (n = 3 in each group, in duplicate) were assayed by q-RT-PCR using the Fluidigm Dynamic Array Integrated Fluidic Circuits and the BioMark HD system (www.fluidigm.com/biomarksystem.html) as previously described [40, 49]. ..

    Cell Culture:

    Article Title: In vitro evidence that platelet-rich plasma stimulates cellular processes involved in endometrial regeneration.
    Article Snippet: For quantitative RT-PCR analysis, 1 μg of RNA was converted to complementary DNA (cDNA) using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, USA). .. Quantitative RT-PCR with multiplex Fluidigm array All cDNA samples from cultured eSF, eMSC, BM-MSC, and Ishikawa cells in control, aPRP, naPRP, aPPP, and naPPP groups (n = 3 in each group, in duplicate) were assayed by q-RT-PCR using the Fluidigm Dynamic Array Integrated Fluidic Circuits and the BioMark HD system (www.fluidigm.com/biomarksystem.html) as previously described [40, 49]. ..

    Control:

    Article Title: In vitro evidence that platelet-rich plasma stimulates cellular processes involved in endometrial regeneration.
    Article Snippet: For quantitative RT-PCR analysis, 1 μg of RNA was converted to complementary DNA (cDNA) using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, USA). .. Quantitative RT-PCR with multiplex Fluidigm array All cDNA samples from cultured eSF, eMSC, BM-MSC, and Ishikawa cells in control, aPRP, naPRP, aPPP, and naPPP groups (n = 3 in each group, in duplicate) were assayed by q-RT-PCR using the Fluidigm Dynamic Array Integrated Fluidic Circuits and the BioMark HD system (www.fluidigm.com/biomarksystem.html) as previously described [40, 49]. ..



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    Image Search Results


    Comparison of available tools for constructing libraries for next-generation sequencing

    Journal: Biology Methods & Protocols

    Article Title: PrimerPooler: automated primer pooling to prepare library for targeted sequencing

    doi: 10.1093/biomethods/bpx006

    Figure Lengend Snippet: Comparison of available tools for constructing libraries for next-generation sequencing

    Article Snippet: Based on the two DNA samples tested, both 50ng and 75ng starting amounts of DNA yielded very similar results, and similarly there was no obvious benefit when less diluted pre-amplified product (2.5 versus 5-fold dilution) was used for Fluidigm Access Array multiplex PCR ( ).

    Techniques: Comparison, Multiplex Assay, Control

    Coverage of targeted sequence regions by Fluidigm Access Array multiplex PCR and Illumina MiSeQ sequencing. Two FFPE DNA samples were tested under various experimental conditions including 50- and 75-ng DNA for pre-amplication, and 1l of 2.5- and 5.0-fold diluted pre-amplified products for Fluidigm Access Array multiplex PCR. An average of 3269 reads was achieved for the targeted regions, with 95% of targets covered by at least 50 reads, the minimal depth of reads for confident variant calling.

    Journal: Biology Methods & Protocols

    Article Title: PrimerPooler: automated primer pooling to prepare library for targeted sequencing

    doi: 10.1093/biomethods/bpx006

    Figure Lengend Snippet: Coverage of targeted sequence regions by Fluidigm Access Array multiplex PCR and Illumina MiSeQ sequencing. Two FFPE DNA samples were tested under various experimental conditions including 50- and 75-ng DNA for pre-amplication, and 1l of 2.5- and 5.0-fold diluted pre-amplified products for Fluidigm Access Array multiplex PCR. An average of 3269 reads was achieved for the targeted regions, with 95% of targets covered by at least 50 reads, the minimal depth of reads for confident variant calling.

    Article Snippet: Based on the two DNA samples tested, both 50ng and 75ng starting amounts of DNA yielded very similar results, and similarly there was no obvious benefit when less diluted pre-amplified product (2.5 versus 5-fold dilution) was used for Fluidigm Access Array multiplex PCR ( ).

    Techniques: Sequencing, Multiplex Assay, Amplification, Variant Assay