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primer target specific pcr amplification  (fluidigm)


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    Structured Review

    fluidigm primer target specific pcr amplification
    Primer Target Specific Pcr Amplification, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 843 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/access+array+target+specific+primers/Access+Array/pm37657678-56-22-38
    Average 93 stars, based on 843 article reviews
    primer target specific pcr amplification - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Paralleled Dynamics of Arabidopsis Root Exudation and SynCom Assembly in a Controlled Environment
    Article Snippet: PCR1 amplicons were purified using the ChargeSwitch® PCR Clean-Up Kit (Invitrogen, Waltham, MA, USA) on a KingFisherTM FlexTM Purification System (ThermoFisher Scientific, Waltham, MA, USA). .. Unique Access Array barcoding primers (Standard BioTools, CA, USA) were added in a second PCR (PCR2) using 5 μL of purified PCR1 product following previously reported cycling conditions (Rensburg et al., 2025). ..

    Article Title: Argan fruit microbiomes: influence of biogeographic and soil driven environmental factors
    Article Snippet: A second PCR was used to attach Illumina sequencing adapters and index tags. .. This reaction included 5 μL of purified PCR product, 2.5 μL of Fluidigm Access Array Barcode 384, and 1X KAPA HiFi HotStart ReadyMix (Roche Sequencing Solutions), in total volume of 50 μl. ..

    Purification:

    Article Title: Paralleled Dynamics of Arabidopsis Root Exudation and SynCom Assembly in a Controlled Environment
    Article Snippet: PCR1 amplicons were purified using the ChargeSwitch® PCR Clean-Up Kit (Invitrogen, Waltham, MA, USA) on a KingFisherTM FlexTM Purification System (ThermoFisher Scientific, Waltham, MA, USA). .. Unique Access Array barcoding primers (Standard BioTools, CA, USA) were added in a second PCR (PCR2) using 5 μL of purified PCR1 product following previously reported cycling conditions (Rensburg et al., 2025). ..

    Article Title: Argan fruit microbiomes: influence of biogeographic and soil driven environmental factors
    Article Snippet: A second PCR was used to attach Illumina sequencing adapters and index tags. .. This reaction included 5 μL of purified PCR product, 2.5 μL of Fluidigm Access Array Barcode 384, and 1X KAPA HiFi HotStart ReadyMix (Roche Sequencing Solutions), in total volume of 50 μl. ..

    High Throughput Screening Assay:

    Article Title: High-frequency oscillatory ventilation during physiological-based cord clamping attenuates inflammation in preterm lambs.
    Article Snippet: 10.1136/arch d isch ild -2025-329610 o n is C h ild F etal N eo n atal E d : first p u b lish ed as F3Benincasa BC, et al. Arch Dis Child Fetal Neonatal Ed 2026;0:F1–F8. doi:10.1136/archdischild- 2025- 329610 Original research and CD163- positive cells were manually quantified using Fiji/ ImageJ (V.2.14.0, LOCI, Wisconsin, USA) and expressed as total and relative to total cell counts. .. High- throughput real- time quantitative PCR using Fluidigm Access Array System Technology (Fluidigm, California, USA) and TaqMan primers (Thermo Fisher Scientific, Massachusetts, USA) was undertaken to quantify messenger RNA (mRNA) expression of 20 curated genes (online supplemental table 1). ..

    Real-time Polymerase Chain Reaction:

    Article Title: High-frequency oscillatory ventilation during physiological-based cord clamping attenuates inflammation in preterm lambs.
    Article Snippet: 10.1136/arch d isch ild -2025-329610 o n is C h ild F etal N eo n atal E d : first p u b lish ed as F3Benincasa BC, et al. Arch Dis Child Fetal Neonatal Ed 2026;0:F1–F8. doi:10.1136/archdischild- 2025- 329610 Original research and CD163- positive cells were manually quantified using Fiji/ ImageJ (V.2.14.0, LOCI, Wisconsin, USA) and expressed as total and relative to total cell counts. .. High- throughput real- time quantitative PCR using Fluidigm Access Array System Technology (Fluidigm, California, USA) and TaqMan primers (Thermo Fisher Scientific, Massachusetts, USA) was undertaken to quantify messenger RNA (mRNA) expression of 20 curated genes (online supplemental table 1). ..

    Expressing:

    Article Title: High-frequency oscillatory ventilation during physiological-based cord clamping attenuates inflammation in preterm lambs.
    Article Snippet: 10.1136/arch d isch ild -2025-329610 o n is C h ild F etal N eo n atal E d : first p u b lish ed as F3Benincasa BC, et al. Arch Dis Child Fetal Neonatal Ed 2026;0:F1–F8. doi:10.1136/archdischild- 2025- 329610 Original research and CD163- positive cells were manually quantified using Fiji/ ImageJ (V.2.14.0, LOCI, Wisconsin, USA) and expressed as total and relative to total cell counts. .. High- throughput real- time quantitative PCR using Fluidigm Access Array System Technology (Fluidigm, California, USA) and TaqMan primers (Thermo Fisher Scientific, Massachusetts, USA) was undertaken to quantify messenger RNA (mRNA) expression of 20 curated genes (online supplemental table 1). ..

    Amplification:

    Article Title: Fungal community structure and network connectivity as indicators of soil health under long-term land use.
    Article Snippet: Sequencing (Miseq) was performed at the Hylabs Laboratory Ltd. (Rehovot, Israel; www.hylabs.co.il) sequencing facility using an Illumina sequencing platform (Illumina Inc., San Diego, CA, USA). .. Protocol PCR2: 2ul sample from PCR1 amplified sample containing CS1/CS2 adaptors was amplified for 10 cycles in 10ul using Fluidigm Access Array Barcode library according to manufacturer’s protocol (2ul barcode per reaction). .. DNA was purified using Kapa Pure Beads at a ratio of 0.65X and quantified with qubit using Denovix DsDNA high sensitivity assay.

    Sequencing:

    Article Title: Genetic variations in AAK1 and ADAM17 associated with circulatory cytokines changes influence COVID-19 susceptibility and severity
    Article Snippet: .. The samples were prepared using the Fluidigm 48.48 Access Array integrated fluidic circuit (IFC) (Fluidigm Europe B.V., Netherlands) for sequencing using the Ion 520TM Chip on an Ion S5 XL Semiconductor (ThermoFisher, USA) as previously described [ ]. ..

    Article Title: Genetic variations in AAK1 and ADAM17 associated with circulatory cytokines changes influence COVID-19 susceptibility and severity.
    Article Snippet: .. The samples were prepared using the Fluidigm 48.48 Access Array integrated fluidic circuit (IFC) (Fluidigm Europe B.V., Netherlands) for sequencing using the Ion 520TM Chip on an Ion S5 XL Semiconductor (ThermoFisher, USA) as previously described (30). ..

    Mutagenesis:

    Article Title: Familial medullary thyroid carcinoma secondary to an SLC30A9 intragenic deletion and translation reinitiation
    Article Snippet: .. The probands from the RET mutation-negative MTC families and all patients with RET mutation-negative sporadic MTC were sequenced for SLC30A9 (NM_006345.4) using the Fluidigm Access Array (Fluidigm, South San Francisco, CA, USA). ..

    Immunopeptidomics:

    Article Title: Dynamics of natural and pharmacologic control of an SIV variant with an envelope trafficking defect
    Article Snippet: .. Briefly, a panel of oligonucleotides flanking the highly polymorphic peptide domains encoded by exon 2 of MHC class I and class II loci were used for multiplex PCRs with an Access Array 48.48 (Standard BioTools) following the manufacturer’s protocol. .. This amplicon library was sequenced on an Illumina MiSeq instrument, and the resulting sequence reads were mapped against a custom reference database of Mane MHC sequences ( https://github.com/dholab/mhc_genotyper ).

    Multiplex Assay:

    Article Title: Dynamics of natural and pharmacologic control of an SIV variant with an envelope trafficking defect
    Article Snippet: .. Briefly, a panel of oligonucleotides flanking the highly polymorphic peptide domains encoded by exon 2 of MHC class I and class II loci were used for multiplex PCRs with an Access Array 48.48 (Standard BioTools) following the manufacturer’s protocol. .. This amplicon library was sequenced on an Illumina MiSeq instrument, and the resulting sequence reads were mapped against a custom reference database of Mane MHC sequences ( https://github.com/dholab/mhc_genotyper ).



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    Comparison of available tools for constructing libraries for next-generation sequencing

    Journal: Biology Methods & Protocols

    Article Title: PrimerPooler: automated primer pooling to prepare library for targeted sequencing

    doi: 10.1093/biomethods/bpx006

    Figure Lengend Snippet: Comparison of available tools for constructing libraries for next-generation sequencing

    Article Snippet: Based on the two DNA samples tested, both 50ng and 75ng starting amounts of DNA yielded very similar results, and similarly there was no obvious benefit when less diluted pre-amplified product (2.5 versus 5-fold dilution) was used for Fluidigm Access Array multiplex PCR ( ).

    Techniques: Comparison, Multiplex Assay, Control

    Coverage of targeted sequence regions by Fluidigm Access Array multiplex PCR and Illumina MiSeQ sequencing. Two FFPE DNA samples were tested under various experimental conditions including 50- and 75-ng DNA for pre-amplication, and 1l of 2.5- and 5.0-fold diluted pre-amplified products for Fluidigm Access Array multiplex PCR. An average of 3269 reads was achieved for the targeted regions, with 95% of targets covered by at least 50 reads, the minimal depth of reads for confident variant calling.

    Journal: Biology Methods & Protocols

    Article Title: PrimerPooler: automated primer pooling to prepare library for targeted sequencing

    doi: 10.1093/biomethods/bpx006

    Figure Lengend Snippet: Coverage of targeted sequence regions by Fluidigm Access Array multiplex PCR and Illumina MiSeQ sequencing. Two FFPE DNA samples were tested under various experimental conditions including 50- and 75-ng DNA for pre-amplication, and 1l of 2.5- and 5.0-fold diluted pre-amplified products for Fluidigm Access Array multiplex PCR. An average of 3269 reads was achieved for the targeted regions, with 95% of targets covered by at least 50 reads, the minimal depth of reads for confident variant calling.

    Article Snippet: Based on the two DNA samples tested, both 50ng and 75ng starting amounts of DNA yielded very similar results, and similarly there was no obvious benefit when less diluted pre-amplified product (2.5 versus 5-fold dilution) was used for Fluidigm Access Array multiplex PCR ( ).

    Techniques: Sequencing, Multiplex Assay, Amplification, Variant Assay