access array barcode library for illumina primers (fluidigm)
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Access Array Barcode Library For Illumina Primers, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 843 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/access+array+barcode+primers/Access+Array/pmc11317036-64-3-12
Average 93 stars, based on 843 article reviews
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1) Product Images from "Quantitating primer-template interactions using deconstructed PCR"
Article Title: Quantitating primer-template interactions using deconstructed PCR
Journal: PeerJ
doi: 10.7717/peerj.17787
Figure Legend Snippet: CS1, common sequence 1 linker sequence. CS2, common sequence 2 linker sequence. BC, barcode. F, Forward primer. R, Reverse primer, P5/P7, Illumina primers, PE1/PE2, Fluidigm Access Array Barcode Library Illumina adapters. In stage 1 (linear copying only), individual samples are cycled for four cycles with locus-specific primers and Fluidigm barcoded primers. Subsequently, all reactions are pooled and purified together, and then amplified with Illumina P5 and P7 primers in stage 2 (exponential amplification with primers targeting linker sequences). During stage 1, linear copying of templates leads to products which contain Illumina sequencing adapters, sample-specific barcodes, and the locus-specific region of interest. Only fragments with Illumina adapters and barcodes are exponentially amplified in stage 2. Locus-specific primer sequences can be modified as needed.
Techniques Used: Sequencing, Purification, Amplification, Modification
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