Review



ac-devd-afc fluorogenic substrate  (Biomol GmbH)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Biomol GmbH ac-devd-afc fluorogenic substrate
    Ac Devd Afc Fluorogenic Substrate, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ac-devd-afc+substrate/fluorogenic+substrate+ac+devd+amc/pmc03546013-207-7-10
    Average 90 stars, based on 1 article reviews
    ac-devd-afc fluorogenic substrate - by Bioz Stars, 2026-11
    90/100 stars

    Images

    Related Articles

    other:

    Article Title: Estrogens protect bone mass by inhibiting NAD + metabolism in osteoclasts
    Article Snippet: Caspase-3 activity was determined by measuring the degradation of the fluorescent substrate DEVD-AFC (Biomol Research Labs, Plymouth, PA) and protein concentration was measured by a Bio-Rad detergent–compatible kit (Bio-Rad, Hercules, CA), as described previously [10].

    Article Title: Estrogens decrease osteoclast number by attenuating mitochondria oxidative phosphorylation and ATP production in early osteoclast precursors
    Article Snippet: Caspase-3 activity was measured by determining the degradation of the fluorometric substrate DEVD-AFC (Biomol Research Labs, Plymouth, PA) and protein concentration was measured using a Bio-Rad detergent–compatible kit (Bio-Rad, Hercules, CA), as described previously , .

    Article Title: Osteocyte RANKL is required for cortical bone loss with age and is induced by senescence
    Article Snippet: Caspase-3 activity was measured by quantifying the degradation of the fluorometric substrate DEVD-AFC (Biomol Research Labs).

    Article Title: ECSIT is essential for RANKL-induced stimulation of mitochondria in osteoclasts and a target for the anti-osteoclastogenic effects of estrogens
    Article Snippet: Caspase-3 activity was determined by measuring the degradation of the fluorescent substrate DEVD-AFC (Biomol Research Labs, Plymouth, PA) and protein concentration was measured by a Bio-Rad detergent–compatible kit (Bio-Rad, Hercules, CA), as described previously ( ).

    Article Title: Identification of a new cell line permissive to porcine reproductive and respiratory syndrome virus infection and replication which is phenotypically distinct from MARC-145 cell line
    Article Snippet: Then, specific substrate for caspases 3/7, the Ac-DEVD-AFC fluorogenic substrate (Biomol Research Laboratories Inc.), was added at a final concentration of 200 μM and the rate of fluorescence released was monitored with a 96-well plate fluorometer (Synergy HT, Biotek, Winooski, VT, USA).

    Article Title: Ac-DEVD-CHO (caspase-3/DEVDase inhibitor) suppresses self-incompatibility–induced programmed cell death in the pollen tubes of petunia ( Petunia hybrida E. Vilm.)
    Article Snippet: The caspase-3-like protease activity in pistil extracts was assayed according to the cleavage of fluorogenic substrate Ac-DEVD-AMC (Ac-Asp-GLUVAL Asp-AMC and AMC, 7-amino-4-methylcoumarin; EMD Biosciences and BIOMOL, USA).

    Activity Assay:

    Article Title: Blocking interleukin-1 receptor type 1 (IL-1R1) signaling in hepatocytes slows down diethylnitrosamine-induced liver tumor growth in obese mice
    Article Snippet: Malondialdehyde (MDA) levels in the whole liver tissue were detected and quantitated using a Lipid Peroxidation (MDA) Colorimetric Assay Kit (BioVision, Milpitas, CA) according to the manufacturer’s protocol. .. Caspase 3 activity was determined by chromogenic peptide substrate cleavage (Ac-DEVD-AFC; Biomol, Hamburg, Germany), as described. .. NF-κB p65 activity was measured in duplicate using a TransAM NF-κB Family Kit (Active Motif, Carlsbad, CA).

    Incubation:

    Article Title: Effects of Wi-Fi (2.45 GHz) Exposure on Apoptosis, Sperm Parameters and Testicular Histomorphometry in Rats: A Time Course Study
    Article Snippet: .. Next 100 ml proteins from lysates were incubated with Ac-DEVD-pNA in a 96-well plate at 37 ̊C for 1 hour, and colorimetric substrate (DEVD-AFC, Biomol, Plymouth Meeting, PA, USA) was preferentially cleaved by caspase- 3. ..



    Similar Products

    casp substrates ac devd afc  (MedChemExpress)
    94
    MedChemExpress casp substrates ac devd afc
    Casp Substrates Ac Devd Afc, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ac-devd-afc+substrate/Ac-DEVD-AFC/10__1016_slash_j__aquaculture__2025__742828-123-1-10
    Average 94 stars, based on 1 article reviews
    casp substrates ac devd afc - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier
    substrate ac-devd-afc #4027914  (Bachem)
    90
    Bachem substrate ac-devd-afc #4027914
    Substrate Ac Devd Afc #4027914, supplied by Bachem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ac-devd-afc+substrate/substrate+ac+devd+afc++4027914/pm40331965-295-1-6
    Average 90 stars, based on 1 article reviews
    substrate ac-devd-afc #4027914 - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    chromogenic peptide substrate cleavage ac-devd-afc  (Biomol GmbH)
    90
    Biomol GmbH chromogenic peptide substrate cleavage ac-devd-afc
    Chromogenic Peptide Substrate Cleavage Ac Devd Afc, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ac-devd-afc+substrate/fluorogenic+substrate+ac+devd+amc/pmc11608749-69-6-11
    Average 90 stars, based on 1 article reviews
    chromogenic peptide substrate cleavage ac-devd-afc - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    ac-devd-afc substrate  (Cayman Chemical)
    90
    Cayman Chemical ac-devd-afc substrate
    Ac Devd Afc Substrate, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ac-devd-afc+substrate/ac+devd+afc+substrate/pm38716700-304-30-31
    Average 90 stars, based on 1 article reviews
    ac-devd-afc substrate - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    ac-devd-afc substrate  (AAT Bioquest)
    90
    AAT Bioquest ac-devd-afc substrate
    Ac Devd Afc Substrate, supplied by AAT Bioquest, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ac-devd-afc+substrate/ac+devd+afc+substrate/pm38688061-82-20-24
    Average 90 stars, based on 1 article reviews
    ac-devd-afc substrate - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    ac-devd-afc substrate  (Enzo Biochem)
    90
    Enzo Biochem ac-devd-afc substrate
    Primary B-CLL cells (A, B) and B-cell cancer lines (C, D) treated with GSK-7975A (GSK, 10, 20, 50 μM) for 48 hours. Alive and dead cells were determined using LIVE/DEAD® staining Kits and analyzed by flow cytometry. (A, C) Representative flow cytometry plots. (B, D) Quantification of the percentage of dead cells in (A, C). Cell mortality was also determined by flow cytometry after annexin V/PI staining (E, F). The percentage of total apoptotic cells was calculated as the percentage of annexin V-FITC positive plus annexin V-FITC/PI-positive population. (G, <t>H)</t> <t>Caspase-3</t> activity determined using <t>Ac-DEVD-AFC</t> as substrate after 24 hours with GSK-7975A (GSK, 0-50 μM) or Synta66 (S66, 0-50 μM) in primary B-CLL cells and B-cell cancer lines. Data from B-cell cancer lines are representative of at least 3 independent experiments. Statistical significance was analyzed by two-tailed, unpaired Student’s t test.
    Ac Devd Afc Substrate, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ac-devd-afc+substrate/ac+devd+afc+substrate/bio_rxiv__2023__12__12__571243-132-14-16
    Average 90 stars, based on 1 article reviews
    ac-devd-afc substrate - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    caspase‐3,7 substrate (ac‐devd‐afc  (Millipore)
    90
    Millipore caspase‐3,7 substrate (ac‐devd‐afc
    Primary B-CLL cells (A, B) and B-cell cancer lines (C, D) treated with GSK-7975A (GSK, 10, 20, 50 μM) for 48 hours. Alive and dead cells were determined using LIVE/DEAD® staining Kits and analyzed by flow cytometry. (A, C) Representative flow cytometry plots. (B, D) Quantification of the percentage of dead cells in (A, C). Cell mortality was also determined by flow cytometry after annexin V/PI staining (E, F). The percentage of total apoptotic cells was calculated as the percentage of annexin V-FITC positive plus annexin V-FITC/PI-positive population. (G, <t>H)</t> <t>Caspase-3</t> activity determined using <t>Ac-DEVD-AFC</t> as substrate after 24 hours with GSK-7975A (GSK, 0-50 μM) or Synta66 (S66, 0-50 μM) in primary B-CLL cells and B-cell cancer lines. Data from B-cell cancer lines are representative of at least 3 independent experiments. Statistical significance was analyzed by two-tailed, unpaired Student’s t test.
    Caspase‐3,7 Substrate (Ac‐Devd‐Afc, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ac-devd-afc+substrate/caspase+3+7+substrate++ac+devd+afc/pm37797174-439-39-50
    Average 90 stars, based on 1 article reviews
    caspase‐3,7 substrate (ac‐devd‐afc - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    ac-devd-afc caspase-3 substrate  (Cayman Chemical)
    90
    Cayman Chemical ac-devd-afc caspase-3 substrate
    Primary B-CLL cells (A, B) and B-cell cancer lines (C, D) treated with GSK-7975A (GSK, 10, 20, 50 μM) for 48 hours. Alive and dead cells were determined using LIVE/DEAD® staining Kits and analyzed by flow cytometry. (A, C) Representative flow cytometry plots. (B, D) Quantification of the percentage of dead cells in (A, C). Cell mortality was also determined by flow cytometry after annexin V/PI staining (E, F). The percentage of total apoptotic cells was calculated as the percentage of annexin V-FITC positive plus annexin V-FITC/PI-positive population. (G, <t>H)</t> <t>Caspase-3</t> activity determined using <t>Ac-DEVD-AFC</t> as substrate after 24 hours with GSK-7975A (GSK, 0-50 μM) or Synta66 (S66, 0-50 μM) in primary B-CLL cells and B-cell cancer lines. Data from B-cell cancer lines are representative of at least 3 independent experiments. Statistical significance was analyzed by two-tailed, unpaired Student’s t test.
    Ac Devd Afc Caspase 3 Substrate, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ac-devd-afc+substrate/ac+devd+amc+fluorogenic+substrate/pmc10294886-46-29-30
    Average 90 stars, based on 1 article reviews
    ac-devd-afc caspase-3 substrate - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    caspase substrates ac-devd-afc  (Tocris)
    90
    Tocris caspase substrates ac-devd-afc
    Primary B-CLL cells (A, B) and B-cell cancer lines (C, D) treated with GSK-7975A (GSK, 10, 20, 50 μM) for 48 hours. Alive and dead cells were determined using LIVE/DEAD® staining Kits and analyzed by flow cytometry. (A, C) Representative flow cytometry plots. (B, D) Quantification of the percentage of dead cells in (A, C). Cell mortality was also determined by flow cytometry after annexin V/PI staining (E, F). The percentage of total apoptotic cells was calculated as the percentage of annexin V-FITC positive plus annexin V-FITC/PI-positive population. (G, <t>H)</t> <t>Caspase-3</t> activity determined using <t>Ac-DEVD-AFC</t> as substrate after 24 hours with GSK-7975A (GSK, 0-50 μM) or Synta66 (S66, 0-50 μM) in primary B-CLL cells and B-cell cancer lines. Data from B-cell cancer lines are representative of at least 3 independent experiments. Statistical significance was analyzed by two-tailed, unpaired Student’s t test.
    Caspase Substrates Ac Devd Afc, supplied by Tocris, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ac-devd-afc+substrate/caspase+substrates+ac+devd+afc/pm36982628-415-7-15
    Average 90 stars, based on 1 article reviews
    caspase substrates ac-devd-afc - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    ac-devd-afc fluorogenic substrate  (Biomol GmbH)
    90
    Biomol GmbH ac-devd-afc fluorogenic substrate
    Primary B-CLL cells (A, B) and B-cell cancer lines (C, D) treated with GSK-7975A (GSK, 10, 20, 50 μM) for 48 hours. Alive and dead cells were determined using LIVE/DEAD® staining Kits and analyzed by flow cytometry. (A, C) Representative flow cytometry plots. (B, D) Quantification of the percentage of dead cells in (A, C). Cell mortality was also determined by flow cytometry after annexin V/PI staining (E, F). The percentage of total apoptotic cells was calculated as the percentage of annexin V-FITC positive plus annexin V-FITC/PI-positive population. (G, <t>H)</t> <t>Caspase-3</t> activity determined using <t>Ac-DEVD-AFC</t> as substrate after 24 hours with GSK-7975A (GSK, 0-50 μM) or Synta66 (S66, 0-50 μM) in primary B-CLL cells and B-cell cancer lines. Data from B-cell cancer lines are representative of at least 3 independent experiments. Statistical significance was analyzed by two-tailed, unpaired Student’s t test.
    Ac Devd Afc Fluorogenic Substrate, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ac-devd-afc+substrate/fluorogenic+substrate+ac+devd+amc/pmc03546013-207-7-10
    Average 90 stars, based on 1 article reviews
    ac-devd-afc fluorogenic substrate - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Primary B-CLL cells (A, B) and B-cell cancer lines (C, D) treated with GSK-7975A (GSK, 10, 20, 50 μM) for 48 hours. Alive and dead cells were determined using LIVE/DEAD® staining Kits and analyzed by flow cytometry. (A, C) Representative flow cytometry plots. (B, D) Quantification of the percentage of dead cells in (A, C). Cell mortality was also determined by flow cytometry after annexin V/PI staining (E, F). The percentage of total apoptotic cells was calculated as the percentage of annexin V-FITC positive plus annexin V-FITC/PI-positive population. (G, H) Caspase-3 activity determined using Ac-DEVD-AFC as substrate after 24 hours with GSK-7975A (GSK, 0-50 μM) or Synta66 (S66, 0-50 μM) in primary B-CLL cells and B-cell cancer lines. Data from B-cell cancer lines are representative of at least 3 independent experiments. Statistical significance was analyzed by two-tailed, unpaired Student’s t test.

    Journal: bioRxiv

    Article Title: Blocking Orai1 constitutive activity inhibits B-cell cancer migration and synergistically acts with drugs to reduce B-CLL cell survival

    doi: 10.1101/2023.12.12.571243

    Figure Lengend Snippet: Primary B-CLL cells (A, B) and B-cell cancer lines (C, D) treated with GSK-7975A (GSK, 10, 20, 50 μM) for 48 hours. Alive and dead cells were determined using LIVE/DEAD® staining Kits and analyzed by flow cytometry. (A, C) Representative flow cytometry plots. (B, D) Quantification of the percentage of dead cells in (A, C). Cell mortality was also determined by flow cytometry after annexin V/PI staining (E, F). The percentage of total apoptotic cells was calculated as the percentage of annexin V-FITC positive plus annexin V-FITC/PI-positive population. (G, H) Caspase-3 activity determined using Ac-DEVD-AFC as substrate after 24 hours with GSK-7975A (GSK, 0-50 μM) or Synta66 (S66, 0-50 μM) in primary B-CLL cells and B-cell cancer lines. Data from B-cell cancer lines are representative of at least 3 independent experiments. Statistical significance was analyzed by two-tailed, unpaired Student’s t test.

    Article Snippet: As reported and described previously [ ], activity of caspase-3 was performed by using Ac-DEVD-AFC substrate (Enzo Life Sciences, France).

    Techniques: Staining, Flow Cytometry, Activity Assay, Two Tailed Test