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ac-devd-afc caspase-3 substrate  (Cayman Chemical)


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    Structured Review

    Cayman Chemical ac-devd-afc caspase-3 substrate
    Ac Devd Afc Caspase 3 Substrate, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ac-devd-afc+substrate/ac+devd+amc+fluorogenic+substrate/pmc10294886-46-29-30
    Average 90 stars, based on 1 article reviews
    ac-devd-afc caspase-3 substrate - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: Anticancer potential of nerolidol on acute lymphoblastic leukemia cells through the interactions with the NF-κB/STAT-3 and PI3K/Akt signaling pathways.
    Article Snippet: The activity of the caspases caspase-3 (cat. No. 32203), caspase-9 (cat. No. 13915) and caspase-8 (cat. No. 13913) was determined with an enzyme-linked immunosorbent assay (ELISA) kit procured from Cayman Chemical (Ann Arbor, USA).

    Article Title: Perisurgical colony stimulating factor one treatment ameliorates liver ischaemia/reperfusion injury in rats
    Article Snippet: The fluorogenic caspase 3 substrate Ac-DEVD-AMC (Cayman, 14986) (10 mmol/L) was dissolved in DMSO and added to initiate the reaction, followed by an incubation for 1 hour at 37 °C.

    Article Title: Ruthenium(II) polypyridyl complexes with benzoxazole derivatives and non-innocent ligands as effective antioxidants in human neuroblasts.
    Article Snippet: Ruthenium(II) polypyridyl complexes continue to raise increasing interest for the encouraging results in several biomedical areas.. Considering their vast chemical-physical repertoire, in particular the possibility to switch from the sensitization of reactive oxygen species (ROS) to ROS-scavenging abilities by tuning the nature of their ligands, it is therefore surprising that their potential as antioxidants has not been largely investigated so far.. Herein, we explored the antioxidant behaviour of the novel ruthenium compound [Ru(dbpy)(2,3-DAN)Cl]PF6 (Ru1), featuring a benzoxazole derivative (dpby = 2,6-bis(4-methyl-2-benzoxazolyl)pyridine) and the noninnocent 2,3-diamminonaftalene (2,3-DAN) ligand, along with the reference tpy-containing analogue [Ru(tpy)(2,3-DAN)Cl]PF6 (Ru2) (tpy = 2,2’:6’,2’’-terpyridine).

    Article Title: Perisurgical Colony Stimulating Factor 1 (CSF1) treatment ameliorates liver ischaemia/reperfusion injury in rats
    Article Snippet: The fluorogenic caspase 3 substrate Ac-DEVD-AMC (Cayman, 14986) (10 mM) was dissolved in DMSO and added to initiate the reaction, followed by an incubation for 1 h at 37 °C.

    Incubation:

    Article Title: HES1 potentiates high salt stress response as an enhancer of NFAT5-DNA binding
    Article Snippet: .. Supernatants of centrifuged cell extracts were mixed with caspase 3 substrate (Cayman CHEMICAL), PBS, DTT, and 2× Reaction Buffer (BioVision) in a black microplate and incubated at 37 °C for 2 h. Caspase activity was read at 400 nm/505 nm (Excitation/Emission) using the Varioskan Flash. ..

    Article Title: New Difunctional Derivatives of Betulin: Preparation, Characterization and Antiproliferative Potential.
    Article Snippet: .. After the incubation, 40 μL of each sample was transferred to a white 96-well plate (Corning, USA) containing 160 μL of the reaction buffer (20 mM HEPES; 10% sucrose; 100 mM NaCl; 1 mM EDTA; 10 mM DTT; and 0.02% Trition X-100, pH 7.3) (HIIET, Wrocław, Poland) with a 9 μM AC-DEVD-AMC (Cayman Chemical, Ann Arbor, MI, USA) fluorogenic substrate (λex = 360 nm, λem = 460 nm). .. The correlation of the fluorescence increase with the caspase-3/7 level was continuously recorded at 37 ◦C for 120 min using Biotek Synergy H4 (Biokom, Warsaw, Poland).

    Article Title: New Difunctional Derivatives of Betulin: Preparation, Characterization and Antiproliferative Potential
    Article Snippet: .. After the incubation, 40 μL of each sample was transferred to a white 96-well plate (Corning, USA) containing 160 μL of the reaction buffer (20 mM HEPES; 10% sucrose; 100 mM NaCl; 1 mM EDTA; 10 mM DTT; and 0.02% Trition X-100, pH 7.3) (HIIET, Wrocław, Poland) with a 9 μM AC-DEVD-AMC (Cayman Chemical, Ann Arbor, MI, USA) fluorogenic substrate (λ ex = 360 nm, λ em = 460 nm). .. The correlation of the fluorescence increase with the caspase-3/7 level was continuously recorded at 37 °C for 120 min using Biotek Synergy H4 (Biokom, Warsaw, Poland).

    Activity Assay:

    Article Title: HES1 potentiates high salt stress response as an enhancer of NFAT5-DNA binding
    Article Snippet: .. Supernatants of centrifuged cell extracts were mixed with caspase 3 substrate (Cayman CHEMICAL), PBS, DTT, and 2× Reaction Buffer (BioVision) in a black microplate and incubated at 37 °C for 2 h. Caspase activity was read at 400 nm/505 nm (Excitation/Emission) using the Varioskan Flash. ..



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    Image Search Results


    Primary B-CLL cells (A, B) and B-cell cancer lines (C, D) treated with GSK-7975A (GSK, 10, 20, 50 μM) for 48 hours. Alive and dead cells were determined using LIVE/DEAD® staining Kits and analyzed by flow cytometry. (A, C) Representative flow cytometry plots. (B, D) Quantification of the percentage of dead cells in (A, C). Cell mortality was also determined by flow cytometry after annexin V/PI staining (E, F). The percentage of total apoptotic cells was calculated as the percentage of annexin V-FITC positive plus annexin V-FITC/PI-positive population. (G, H) Caspase-3 activity determined using Ac-DEVD-AFC as substrate after 24 hours with GSK-7975A (GSK, 0-50 μM) or Synta66 (S66, 0-50 μM) in primary B-CLL cells and B-cell cancer lines. Data from B-cell cancer lines are representative of at least 3 independent experiments. Statistical significance was analyzed by two-tailed, unpaired Student’s t test.

    Journal: bioRxiv

    Article Title: Blocking Orai1 constitutive activity inhibits B-cell cancer migration and synergistically acts with drugs to reduce B-CLL cell survival

    doi: 10.1101/2023.12.12.571243

    Figure Lengend Snippet: Primary B-CLL cells (A, B) and B-cell cancer lines (C, D) treated with GSK-7975A (GSK, 10, 20, 50 μM) for 48 hours. Alive and dead cells were determined using LIVE/DEAD® staining Kits and analyzed by flow cytometry. (A, C) Representative flow cytometry plots. (B, D) Quantification of the percentage of dead cells in (A, C). Cell mortality was also determined by flow cytometry after annexin V/PI staining (E, F). The percentage of total apoptotic cells was calculated as the percentage of annexin V-FITC positive plus annexin V-FITC/PI-positive population. (G, H) Caspase-3 activity determined using Ac-DEVD-AFC as substrate after 24 hours with GSK-7975A (GSK, 0-50 μM) or Synta66 (S66, 0-50 μM) in primary B-CLL cells and B-cell cancer lines. Data from B-cell cancer lines are representative of at least 3 independent experiments. Statistical significance was analyzed by two-tailed, unpaired Student’s t test.

    Article Snippet: As reported and described previously [ ], activity of caspase-3 was performed by using Ac-DEVD-AFC substrate (Enzo Life Sciences, France).

    Techniques: Staining, Flow Cytometry, Activity Assay, Two Tailed Test