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ac-devd-afc fluorogenic substrate (Biomol GmbH)

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Biomol GmbH ac-devd-afc fluorogenic substrate
Ac Devd Afc Fluorogenic Substrate, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ac-devd-afc+fluorogenic+substrate/fluorogenic+substrate+ac+devd+amc/pmc03546013-207-7-10
Average 90 stars, based on 1 article reviews

ac-devd-afc fluorogenic substrate - by Bioz Stars, 2026-09

90/100 stars

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Article Title: Estrogens protect bone mass by inhibiting NAD + metabolism in osteoclasts
Article Snippet: Caspase-3 activity was determined by measuring the degradation of the fluorescent substrate DEVD-AFC (Biomol Research Labs, Plymouth, PA) and protein concentration was measured by a Bio-Rad detergent–compatible kit (Bio-Rad, Hercules, CA), as described previously [10].

Article Title: Estrogens decrease osteoclast number by attenuating mitochondria oxidative phosphorylation and ATP production in early osteoclast precursors
Article Snippet: Caspase-3 activity was measured by determining the degradation of the fluorometric substrate DEVD-AFC (Biomol Research Labs, Plymouth, PA) and protein concentration was measured using a Bio-Rad detergent–compatible kit (Bio-Rad, Hercules, CA), as described previously , .

Article Title: Osteocyte RANKL is required for cortical bone loss with age and is induced by senescence
Article Snippet: Caspase-3 activity was measured by quantifying the degradation of the fluorometric substrate DEVD-AFC (Biomol Research Labs).

Article Title: ECSIT is essential for RANKL-induced stimulation of mitochondria in osteoclasts and a target for the anti-osteoclastogenic effects of estrogens
Article Snippet: Caspase-3 activity was determined by measuring the degradation of the fluorescent substrate DEVD-AFC (Biomol Research Labs, Plymouth, PA) and protein concentration was measured by a Bio-Rad detergent–compatible kit (Bio-Rad, Hercules, CA), as described previously ( ).

Article Title: Identification of a new cell line permissive to porcine reproductive and respiratory syndrome virus infection and replication which is phenotypically distinct from MARC-145 cell line
Article Snippet: Then, specific substrate for caspases 3/7, the Ac-DEVD-AFC fluorogenic substrate (Biomol Research Laboratories Inc.), was added at a final concentration of 200 μM and the rate of fluorescence released was monitored with a 96-well plate fluorometer (Synergy HT, Biotek, Winooski, VT, USA).

Article Title: Ac-DEVD-CHO (caspase-3/DEVDase inhibitor) suppresses self-incompatibility–induced programmed cell death in the pollen tubes of petunia ( Petunia hybrida E. Vilm.)
Article Snippet: The caspase-3-like protease activity in pistil extracts was assayed according to the cleavage of fluorogenic substrate Ac-DEVD-AMC (Ac-Asp-GLUVAL Asp-AMC and AMC, 7-amino-4-methylcoumarin; EMD Biosciences and BIOMOL, USA).

Activity Assay:

Article Title: Blocking interleukin-1 receptor type 1 (IL-1R1) signaling in hepatocytes slows down diethylnitrosamine-induced liver tumor growth in obese mice
Article Snippet: Malondialdehyde (MDA) levels in the whole liver tissue were detected and quantitated using a Lipid Peroxidation (MDA) Colorimetric Assay Kit (BioVision, Milpitas, CA) according to the manufacturer’s protocol. .. Caspase 3 activity was determined by chromogenic peptide substrate cleavage (Ac-DEVD-AFC; Biomol, Hamburg, Germany), as described. .. NF-κB p65 activity was measured in duplicate using a TransAM NF-κB Family Kit (Active Motif, Carlsbad, CA).

Incubation:

Article Title: Effects of Wi-Fi (2.45 GHz) Exposure on Apoptosis, Sperm Parameters and Testicular Histomorphometry in Rats: A Time Course Study
Article Snippet: .. Next 100 ml proteins from lysates were incubated with Ac-DEVD-pNA in a 96-well plate at 37 ̊C for 1 hour, and colorimetric substrate (DEVD-AFC, Biomol, Plymouth Meeting, PA, USA) was preferentially cleaved by caspase- 3. ..