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a498  (OriGene)


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    Structured Review

    OriGene a498
    a , b Expression of REGγ in RCC tissues (T) and normal kidney tissues (N) as detected by IHC ( a ) and WB ( b ). c Expression of REGγ in four RCC cell lines <t>(A498,</t> 786-O, ACHN, and caki-1) and the normal renal tubular epithelial cell line (HK-2) as detected by western blot. d Comparison of REGγ expression in different Fuhrman grades I–IV of RCC tissue samples via IHC staining (left panel: magnification ×200, scale bar = 50 μm; right panel: magnification ×400, scale bar = 20 μm). e REGγ (PSME3) expression (median of expression intensity) in different pathological types and grades of RCC derived from Oncomine database ( https://www.oncomine.org/ ). f Kaplan–Meier analysis of the correlation between REGγ expression and the survival time in RCC patients. Cases were classified into lower expression group and higher expression group as described in methods. g Prognosis of RCC (KIRC) patients with high or low expression of REGγ (PSME3) derived from OncoLnc database ( https://www.oncolnc.org/ )
    A498, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a498/A498+(Human+Kidney+Carcinoma)+Whole+Cell+Lysate/pmc05967313-80-9-27
    Average 90 stars, based on 4 article reviews
    a498 - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "REGγ deficiency suppresses tumor progression via stabilizing CK1ε in renal cell carcinoma"

    Article Title: REGγ deficiency suppresses tumor progression via stabilizing CK1ε in renal cell carcinoma

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-018-0646-2

    a , b Expression of REGγ in RCC tissues (T) and normal kidney tissues (N) as detected by IHC ( a ) and WB ( b ). c Expression of REGγ in four RCC cell lines (A498, 786-O, ACHN, and caki-1) and the normal renal tubular epithelial cell line (HK-2) as detected by western blot. d Comparison of REGγ expression in different Fuhrman grades I–IV of RCC tissue samples via IHC staining (left panel: magnification ×200, scale bar = 50 μm; right panel: magnification ×400, scale bar = 20 μm). e REGγ (PSME3) expression (median of expression intensity) in different pathological types and grades of RCC derived from Oncomine database ( https://www.oncomine.org/ ). f Kaplan–Meier analysis of the correlation between REGγ expression and the survival time in RCC patients. Cases were classified into lower expression group and higher expression group as described in methods. g Prognosis of RCC (KIRC) patients with high or low expression of REGγ (PSME3) derived from OncoLnc database ( https://www.oncolnc.org/ )
    Figure Legend Snippet: a , b Expression of REGγ in RCC tissues (T) and normal kidney tissues (N) as detected by IHC ( a ) and WB ( b ). c Expression of REGγ in four RCC cell lines (A498, 786-O, ACHN, and caki-1) and the normal renal tubular epithelial cell line (HK-2) as detected by western blot. d Comparison of REGγ expression in different Fuhrman grades I–IV of RCC tissue samples via IHC staining (left panel: magnification ×200, scale bar = 50 μm; right panel: magnification ×400, scale bar = 20 μm). e REGγ (PSME3) expression (median of expression intensity) in different pathological types and grades of RCC derived from Oncomine database ( https://www.oncomine.org/ ). f Kaplan–Meier analysis of the correlation between REGγ expression and the survival time in RCC patients. Cases were classified into lower expression group and higher expression group as described in methods. g Prognosis of RCC (KIRC) patients with high or low expression of REGγ (PSME3) derived from OncoLnc database ( https://www.oncolnc.org/ )

    Techniques Used: Expressing, Western Blot, Immunohistochemistry, Derivative Assay

    a PSME3 upregulation is closely correlated with renal cell carcinoma revealed by KEGG pathway analysis. b GSEA indicated that cell proliferation and anti-apoptosis were positively correlated with elevated PSME3 expression in RCC from database GSE89563 (GO_0008284 and GO_0006915). NES, normalized enrichment score. c Stable knockdown of REGγ (shREGγ) in RCC cell lines (ACHN and A498) confirmed by WB. d , e Effect of shREGγ on RCC cells growth as determined by MTT assay. f Representative images of colony formation of RCC cells after transfection of shREGγ versus shNC. g , h Representative images of EdU incorporation assay after transfection of shREGγ versus shNC. i , j Representative flow cytometry plots of cell cycle distribution from ACHN and A498 cells transfected with shREGγ and shNC. k , l Apoptosis rate from ACHN and A498 cells after transfected with shREGγ and shNC as detected by flow cytometry. Data are shown as mean ± SD. * P < 0.05
    Figure Legend Snippet: a PSME3 upregulation is closely correlated with renal cell carcinoma revealed by KEGG pathway analysis. b GSEA indicated that cell proliferation and anti-apoptosis were positively correlated with elevated PSME3 expression in RCC from database GSE89563 (GO_0008284 and GO_0006915). NES, normalized enrichment score. c Stable knockdown of REGγ (shREGγ) in RCC cell lines (ACHN and A498) confirmed by WB. d , e Effect of shREGγ on RCC cells growth as determined by MTT assay. f Representative images of colony formation of RCC cells after transfection of shREGγ versus shNC. g , h Representative images of EdU incorporation assay after transfection of shREGγ versus shNC. i , j Representative flow cytometry plots of cell cycle distribution from ACHN and A498 cells transfected with shREGγ and shNC. k , l Apoptosis rate from ACHN and A498 cells after transfected with shREGγ and shNC as detected by flow cytometry. Data are shown as mean ± SD. * P < 0.05

    Techniques Used: Expressing, MTT Assay, Transfection, Flow Cytometry

    a , b Representative images and the relative quantification of wound-healing assay in RCC cells transfected with shREGγ and shNC. c , d Representative images and the relative quantification of transwell invasion assay in ACHN and A498 cells transfected with shREGγ and shNC. Data are shown as mean ± SD. * P < 0.05
    Figure Legend Snippet: a , b Representative images and the relative quantification of wound-healing assay in RCC cells transfected with shREGγ and shNC. c , d Representative images and the relative quantification of transwell invasion assay in ACHN and A498 cells transfected with shREGγ and shNC. Data are shown as mean ± SD. * P < 0.05

    Techniques Used: Wound Healing Assay, Transfection, Transwell Invasion Assay

    Related Articles

    Transfection:

    Article Title: MicroRNA-185 inhibits cell proliferation and induces cell apoptosis by targeting VEGFA directly in von Hippel-Lindau-inactivated clear cell renal cell carcinoma.
    Article Snippet: Objectives: The von Hippel-Lindau (VHL) gene acts as a tumor suppressor in most clear cell renal cell carcinomas (ccRCCs).. Tumor growth in ccRCCs relies on many factors that result from the loss of VHL.. This study aims to identify new microRNAs with therapeutic potential for VHL-inactivated ccRCCs.

    Small Interfering RNA:

    Article Title: MicroRNA-185 inhibits cell proliferation and induces cell apoptosis by targeting VEGFA directly in von Hippel-Lindau-inactivated clear cell renal cell carcinoma.
    Article Snippet: Objectives: The von Hippel-Lindau (VHL) gene acts as a tumor suppressor in most clear cell renal cell carcinomas (ccRCCs).. Tumor growth in ccRCCs relies on many factors that result from the loss of VHL.. This study aims to identify new microRNAs with therapeutic potential for VHL-inactivated ccRCCs.

    Plasmid Preparation:

    Article Title: MicroRNA-185 inhibits cell proliferation and induces cell apoptosis by targeting VEGFA directly in von Hippel-Lindau-inactivated clear cell renal cell carcinoma.
    Article Snippet: Objectives: The von Hippel-Lindau (VHL) gene acts as a tumor suppressor in most clear cell renal cell carcinomas (ccRCCs).. Tumor growth in ccRCCs relies on many factors that result from the loss of VHL.. This study aims to identify new microRNAs with therapeutic potential for VHL-inactivated ccRCCs.

    Knockdown:

    Article Title: REGγ deficiency suppresses tumor progression via stabilizing CK1ε in renal cell carcinoma
    Article Snippet: .. The stable REGγ knockdown RCC cell lines ACHN and A498 were generated by integration of retroviral shREGγ vectors specific for REGγ or a control gene (GFP) from OriGene (Rockville, MD), which was performed as previously described . .. Transduced cells were selected in puromycin (Invitrogen, CA, USA).

    Generated:

    Article Title: REGγ deficiency suppresses tumor progression via stabilizing CK1ε in renal cell carcinoma
    Article Snippet: .. The stable REGγ knockdown RCC cell lines ACHN and A498 were generated by integration of retroviral shREGγ vectors specific for REGγ or a control gene (GFP) from OriGene (Rockville, MD), which was performed as previously described . .. Transduced cells were selected in puromycin (Invitrogen, CA, USA).

    Retroviral:

    Article Title: REGγ deficiency suppresses tumor progression via stabilizing CK1ε in renal cell carcinoma
    Article Snippet: .. The stable REGγ knockdown RCC cell lines ACHN and A498 were generated by integration of retroviral shREGγ vectors specific for REGγ or a control gene (GFP) from OriGene (Rockville, MD), which was performed as previously described . .. Transduced cells were selected in puromycin (Invitrogen, CA, USA).

    Control:

    Article Title: REGγ deficiency suppresses tumor progression via stabilizing CK1ε in renal cell carcinoma
    Article Snippet: .. The stable REGγ knockdown RCC cell lines ACHN and A498 were generated by integration of retroviral shREGγ vectors specific for REGγ or a control gene (GFP) from OriGene (Rockville, MD), which was performed as previously described . .. Transduced cells were selected in puromycin (Invitrogen, CA, USA).



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    ( A ) Schematic illustration of the experimental setup with four groups: (i) unmodified 293T cells, (ii) 293T cells modified with GFP only, (iii) 293T cells modified with TRAIL + GFP , and (iv) cell-based microrobots—293T cells modified with TRAIL + GFP and then conjugated to magnetic Janus particles. 293T cells were seeded in the upper chamber of a transwell system; healthy hAMSCs or <t>A498</t> cancer cells were seeded in the lower chamber. Cell viability was assessed after 3 days of coculture. Created in BioRender. N. O. Dogan (2026), https://biorender.com/3lke7fg . ( B ) TRAIL secretion was measured in all groups at 48 hours posttransfection. Data are presented as the means ± SD from n = 3 technical replicates. One-way ANOVA with Tukey’s post hoc test; n.s. indicates P ≥ 0.05, and **** P < 0.0001. ( C ) CellTiter-Glo viability assays showing a minimal effect on healthy hAMSC viability across groups, while A498 cancer cells exhibited significant death in both TRAIL + GFP and TRAIL + GFP + particles groups. Data are presented as the means ± SD from n = 10 technical replicates. One-way ANOVA with Tukey’s post hoc test; n.s. indicates P ≥ 0.05; * P < 0.05, ** P < 0.01, and **** P < 0.0001. ( D ) Representative live/dead fluorescence images confirming the selective cytotoxicity of cell-based microrobots on A498 cancer cells, with no detectable harm on healthy hAMSCs. Scale bars, 100 μm.
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    Image Search Results


    ( A ) Schematic illustration of the experimental setup with four groups: (i) unmodified 293T cells, (ii) 293T cells modified with GFP only, (iii) 293T cells modified with TRAIL + GFP , and (iv) cell-based microrobots—293T cells modified with TRAIL + GFP and then conjugated to magnetic Janus particles. 293T cells were seeded in the upper chamber of a transwell system; healthy hAMSCs or A498 cancer cells were seeded in the lower chamber. Cell viability was assessed after 3 days of coculture. Created in BioRender. N. O. Dogan (2026), https://biorender.com/3lke7fg . ( B ) TRAIL secretion was measured in all groups at 48 hours posttransfection. Data are presented as the means ± SD from n = 3 technical replicates. One-way ANOVA with Tukey’s post hoc test; n.s. indicates P ≥ 0.05, and **** P < 0.0001. ( C ) CellTiter-Glo viability assays showing a minimal effect on healthy hAMSC viability across groups, while A498 cancer cells exhibited significant death in both TRAIL + GFP and TRAIL + GFP + particles groups. Data are presented as the means ± SD from n = 10 technical replicates. One-way ANOVA with Tukey’s post hoc test; n.s. indicates P ≥ 0.05; * P < 0.05, ** P < 0.01, and **** P < 0.0001. ( D ) Representative live/dead fluorescence images confirming the selective cytotoxicity of cell-based microrobots on A498 cancer cells, with no detectable harm on healthy hAMSCs. Scale bars, 100 μm.

    Journal: Science Advances

    Article Title: Genetically engineered human cell–based microrobots for selective cancer cell death

    doi: 10.1126/sciadv.aea9831

    Figure Lengend Snippet: ( A ) Schematic illustration of the experimental setup with four groups: (i) unmodified 293T cells, (ii) 293T cells modified with GFP only, (iii) 293T cells modified with TRAIL + GFP , and (iv) cell-based microrobots—293T cells modified with TRAIL + GFP and then conjugated to magnetic Janus particles. 293T cells were seeded in the upper chamber of a transwell system; healthy hAMSCs or A498 cancer cells were seeded in the lower chamber. Cell viability was assessed after 3 days of coculture. Created in BioRender. N. O. Dogan (2026), https://biorender.com/3lke7fg . ( B ) TRAIL secretion was measured in all groups at 48 hours posttransfection. Data are presented as the means ± SD from n = 3 technical replicates. One-way ANOVA with Tukey’s post hoc test; n.s. indicates P ≥ 0.05, and **** P < 0.0001. ( C ) CellTiter-Glo viability assays showing a minimal effect on healthy hAMSC viability across groups, while A498 cancer cells exhibited significant death in both TRAIL + GFP and TRAIL + GFP + particles groups. Data are presented as the means ± SD from n = 10 technical replicates. One-way ANOVA with Tukey’s post hoc test; n.s. indicates P ≥ 0.05; * P < 0.05, ** P < 0.01, and **** P < 0.0001. ( D ) Representative live/dead fluorescence images confirming the selective cytotoxicity of cell-based microrobots on A498 cancer cells, with no detectable harm on healthy hAMSCs. Scale bars, 100 μm.

    Article Snippet: ACHN and A498 human renal adenocarcinomas (ATCC) and ONCO-DG-1 human ovarian adenocarcinomas (DSMZ) were cultivated in Roswell Park Memorial Institute (RPMI) 1640 medium (Gibco).

    Techniques: Modification, Fluorescence

    ( A ) Differential interference contrast imaging demonstrating the magnetic guidance of cell-based microrobots toward a 3D tumor spheroid. Scale bars, 100 μm. ( B ) As shown by live/dead fluorescence imaging, conditioned medium from cell-based microrobots induced increased tumor cell death in 3D A498 renal adenocarcinoma tumor spheroids, unlike medium from unmodified 293T cells. Scale bars, 100 μm. ( C ) CellTiter-Glo 3D assay revealed significantly reduced tumor spheroid viability after 24 hours of incubation with conditioned medium from cell-based microrobots compared to medium from unmodified 293T cells. Data are presented as the means ± SD, n = 6 technical replicates. One-way ANOVA with Tukey’s post hoc test; **** P < 0.0001.

    Journal: Science Advances

    Article Title: Genetically engineered human cell–based microrobots for selective cancer cell death

    doi: 10.1126/sciadv.aea9831

    Figure Lengend Snippet: ( A ) Differential interference contrast imaging demonstrating the magnetic guidance of cell-based microrobots toward a 3D tumor spheroid. Scale bars, 100 μm. ( B ) As shown by live/dead fluorescence imaging, conditioned medium from cell-based microrobots induced increased tumor cell death in 3D A498 renal adenocarcinoma tumor spheroids, unlike medium from unmodified 293T cells. Scale bars, 100 μm. ( C ) CellTiter-Glo 3D assay revealed significantly reduced tumor spheroid viability after 24 hours of incubation with conditioned medium from cell-based microrobots compared to medium from unmodified 293T cells. Data are presented as the means ± SD, n = 6 technical replicates. One-way ANOVA with Tukey’s post hoc test; **** P < 0.0001.

    Article Snippet: ACHN and A498 human renal adenocarcinomas (ATCC) and ONCO-DG-1 human ovarian adenocarcinomas (DSMZ) were cultivated in Roswell Park Memorial Institute (RPMI) 1640 medium (Gibco).

    Techniques: Imaging, Fluorescence, Incubation