Journal: Frontiers in Immunology

Article Title: UPF1-dependent glycolysis driven by LINC00887 facilitates macrophage M2 polarization in clear cell renal cell carcinoma

doi: 10.3389/fimmu.2026.1833383

Figure Lengend Snippet: LINC00887 is highly expressed and its impact on aerobic glycolysis in ccRCC, (A) Analysis of LINC00887 mRNA levels in ccRCC and adjacent normal tissues from TCGA-KIRC dataset. (B) qRT-PCR test of LINC00887 mRNA levels in HK-2, 786-O, Caki-1, and A498 cell lines. (C) KEGG pathway analysis of signaling pathways enriched by LINC00887 in ccRCC. (D) KEGG analysis showing enrichment of LINC00887 in the glycolysis pathway in ccRCC. (E) Pearson correlation analysis between LINC00887 and glycolytic marker genes. 786-O cells were transfected with either oe-NC or oe-LINC00887, respectively. (F) qRT-PCR was performed to verify transfection efficiency. (G) qRT-PCR measurement of glycolytic marker genes LDH-A, HK2, and GLUT1 mRNA levels in each group. (H) immunoblotting analysis of glycolytic marker LDH-A, HK2, and GLUT1 protein levels in each group. (I) Seahorse metabolic analyzer was used to measure ECAR in each group, calculating glycolytic value and glycolytic capacity. (J) Seahorse metabolic analyzer was used to measure OCR in each group, calculating ATP production, and maximal respiration. (K–M) Kits were used to measure glucose consumption (K) , lactate production (L) , and ATP yield (M) . *indicates p < 0.05. ccRCC, Clear cell renal cell carcinoma; qRT-PCR, Quantitative reverse transcription polymerase chain reaction; KEGG, Kyoto Encyclopedia of Genes and Genomes; LDH-A, Lactate dehydrogenase A; HK2, Hexokinase 2; GLUT1, Glucose transporter 1; ECAR, Extracellular acidification rate; OCR, Oxygen consumption rate; ATP, Adenosine triphosphate.

Article Snippet: Caki-1 cells were kept in 5A medium (30-2007, ATCC, USA), 786-O cells in DMEM-H (11965092, Gibco, USA), and A498 cells in EMEM (30-2003, ATCC, USA).

Techniques: Analysis, Quantitative RT-PCR, Protein-Protein interactions, Marker, Transfection, Western Blot, Reverse Transcription, Polymerase Chain Reaction

Journal: Frontiers in Immunology

Article Title: UPF1-dependent glycolysis driven by LINC00887 facilitates macrophage M2 polarization in clear cell renal cell carcinoma

doi: 10.3389/fimmu.2026.1833383

Figure Lengend Snippet: LINC00887-mediated UPF1 suppresses ccRCC malignant progression, (A) mRNA levels of UPF1 in ccRCC tumor tissues versus adjacent normal tissues as analyzed by TCGA-KIRC dataset. (B) qRT-PCR detection of UPF1 mRNA in HK-2, Caki-1, 786-O, and A498 cells. (C) Immunoblotting assessment of UPF1 protein in renal cell lines. (D) CCK-8 assay for cell viability in 786-O cells expressing sh-NC or sh-UPF1 and Caki-1 cells expressing oe-NC or oe-UPF1. (E) Colony formation assay determined cell proliferation in the transfected cells. (F) Scratch assay evaluated cell migration. (G) Transwell assessed cell invasion. (H) Flow cytometry analyzed apoptosis in different cell groups. (I) Flow cytometry examined cell cycle distribution in various cell groups. *, p < 0.05. ccRCC, Clear cell renal cell carcinoma; UPF1, Up-frameshift protein 1. qRT-PCR, Quantitative reverse transcription polymerase chain reaction; TCGA, The cancer genome atlas; KIRC, Kidney renal clear cell carcinoma.

Article Snippet: Caki-1 cells were kept in 5A medium (30-2007, ATCC, USA), 786-O cells in DMEM-H (11965092, Gibco, USA), and A498 cells in EMEM (30-2003, ATCC, USA).

Techniques: Quantitative RT-PCR, Western Blot, CCK-8 Assay, Expressing, Colony Assay, Transfection, Scratch Assay, Migration, Flow Cytometry, Reverse Transcription, Polymerase Chain Reaction

Journal: Science Advances

Article Title: Genetically engineered human cell–based microrobots for selective cancer cell death

doi: 10.1126/sciadv.aea9831

Figure Lengend Snippet: ( A ) Schematic illustration of the experimental setup with four groups: (i) unmodified 293T cells, (ii) 293T cells modified with GFP only, (iii) 293T cells modified with TRAIL + GFP , and (iv) cell-based microrobots—293T cells modified with TRAIL + GFP and then conjugated to magnetic Janus particles. 293T cells were seeded in the upper chamber of a transwell system; healthy hAMSCs or A498 cancer cells were seeded in the lower chamber. Cell viability was assessed after 3 days of coculture. Created in BioRender. N. O. Dogan (2026), https://biorender.com/3lke7fg . ( B ) TRAIL secretion was measured in all groups at 48 hours posttransfection. Data are presented as the means ± SD from n = 3 technical replicates. One-way ANOVA with Tukey’s post hoc test; n.s. indicates P ≥ 0.05, and **** P < 0.0001. ( C ) CellTiter-Glo viability assays showing a minimal effect on healthy hAMSC viability across groups, while A498 cancer cells exhibited significant death in both TRAIL + GFP and TRAIL + GFP + particles groups. Data are presented as the means ± SD from n = 10 technical replicates. One-way ANOVA with Tukey’s post hoc test; n.s. indicates P ≥ 0.05; * P < 0.05, ** P < 0.01, and **** P < 0.0001. ( D ) Representative live/dead fluorescence images confirming the selective cytotoxicity of cell-based microrobots on A498 cancer cells, with no detectable harm on healthy hAMSCs. Scale bars, 100 μm.

Article Snippet: ACHN and A498 human renal adenocarcinomas (ATCC) and ONCO-DG-1 human ovarian adenocarcinomas (DSMZ) were cultivated in Roswell Park Memorial Institute (RPMI) 1640 medium (Gibco).

Techniques: Modification, Fluorescence

Journal: Science Advances

Article Title: Genetically engineered human cell–based microrobots for selective cancer cell death

doi: 10.1126/sciadv.aea9831

Figure Lengend Snippet: ( A ) Differential interference contrast imaging demonstrating the magnetic guidance of cell-based microrobots toward a 3D tumor spheroid. Scale bars, 100 μm. ( B ) As shown by live/dead fluorescence imaging, conditioned medium from cell-based microrobots induced increased tumor cell death in 3D A498 renal adenocarcinoma tumor spheroids, unlike medium from unmodified 293T cells. Scale bars, 100 μm. ( C ) CellTiter-Glo 3D assay revealed significantly reduced tumor spheroid viability after 24 hours of incubation with conditioned medium from cell-based microrobots compared to medium from unmodified 293T cells. Data are presented as the means ± SD, n = 6 technical replicates. One-way ANOVA with Tukey’s post hoc test; **** P < 0.0001.

Article Snippet: ACHN and A498 human renal adenocarcinomas (ATCC) and ONCO-DG-1 human ovarian adenocarcinomas (DSMZ) were cultivated in Roswell Park Memorial Institute (RPMI) 1640 medium (Gibco).

Techniques: Imaging, Fluorescence, Incubation