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lambda fix ii mouse 129/sv genomic library  (Agilent technologies)


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    Agilent technologies lambda fix ii mouse 129/sv genomic library
    Lambda Fix Ii Mouse 129/Sv Genomic Library, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a+lambda+129/pmc04156920-381-16-19
    Average 90 stars, based on 1 article reviews
    lambda fix ii mouse 129/sv genomic library - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Identification and functional characterization of paxillin as a target of protein tyrosine phosphatase receptor T
    Article Snippet: A genomic clone encompassing exons containing the catalytic domains of PTPRT was isolated from the 129/SvJ genomic library (Stratagene).

    Article Title: Genes encoding several poly (ADP-ribose) glycohydrolase (PARG) enzymes, the proteins and fragments thereof, and antibodies immunoreactive therewith
    Article Snippet: A mouse 129/SV genomic library (Stratagene) containing mouse genomic sequences in λ phage was screened using a 2.49 kb fragment of the mouse PARG cDNA as a probe.

    Article Title: Dose-dependent Smad1 , Smad5 and Smad8 signaling in the early mouse embryo
    Article Snippet: A 129/SvJ genomic library (Stratagene) was screened using a full-length Smad8 cDNA clone as a probe to isolate Smad8 genomic fragments.

    Article Title: Abcd2 Is a Strong Modifier of the Metabolic Impairments in Peritoneal Macrophages of Abcd1 -Deficient Mice
    Article Snippet: A mouse 129/SvJ genomic library (λFIXII; Stratagene) was screened with a probe covering nucleotides 16−1,078 of the murine Abcd2 cDNA (GenBankTM Accession No. NM 011994).

    Sequencing:

    Article Title: Mx1-Cre mediated Rgs12 conditional knockout mice exhibit increased bone mass phenotype
    Article Snippet: .. Rgs12 genomic DNA fragments from a 129/Sv genomic library (Stratagene) were screened with a 32 P-labeled 1.0 kb Rgs12 cDNA fragment as a probe and confirmed by restriction mapping and sequencing. ..

    Plasmid Preparation:

    Article Title: Imaging Activity in Neurons and Glia with a Polr2a -based and Cre-dependent GCaMP5G-IRES-tdTomato Reporter Mouse
    Article Snippet: Statistical calculations were performed either with MATLAB (MathWorks Inc., Natick, MA) or GraphPad Prism 6.0 (GraphPad Software, La Jolla, CA). .. The targeting vector shown in was prepared with DNA isolated from a lambda FIX II mouse 129/SV genomic library (Stratagene). ..

    Isolation:

    Article Title: Imaging Activity in Neurons and Glia with a Polr2a -based and Cre-dependent GCaMP5G-IRES-tdTomato Reporter Mouse
    Article Snippet: Statistical calculations were performed either with MATLAB (MathWorks Inc., Natick, MA) or GraphPad Prism 6.0 (GraphPad Software, La Jolla, CA). .. The targeting vector shown in was prepared with DNA isolated from a lambda FIX II mouse 129/SV genomic library (Stratagene). ..

    Article Title: Control of kidney, eye and limb expression of Bmp7 by an enhancer element highly conserved between species
    Article Snippet: Bmp7 cDNA clones isolated from an embryonic kidney library (Stratagene) using the Bmp7 coding sequence probe were sequenced, and their 5’ termini compared to the Bmp7 genomic sequence. .. A phage clone spanning approximately 20 kilobases (kb) surrounding the first exon of Bmp7 was isolated from a 129 SVJ genomic library (Stratagene) by screening using a 32 P-labeled random primed probe representing the first exon of Bmp7 using standard procedures ( Sambrook et al., 1989 ). ..

    Random Primed:

    Article Title: Control of kidney, eye and limb expression of Bmp7 by an enhancer element highly conserved between species
    Article Snippet: Bmp7 cDNA clones isolated from an embryonic kidney library (Stratagene) using the Bmp7 coding sequence probe were sequenced, and their 5’ termini compared to the Bmp7 genomic sequence. .. A phage clone spanning approximately 20 kilobases (kb) surrounding the first exon of Bmp7 was isolated from a 129 SVJ genomic library (Stratagene) by screening using a 32 P-labeled random primed probe representing the first exon of Bmp7 using standard procedures ( Sambrook et al., 1989 ). ..



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    Image Search Results


    Human multiple myeloma cell line U266-ffluc was i.v. (tail vein) injected into NSG mice. After 4 weeks, the mice were randomized into 3 groups comprising 8–9 mice each and treated with i.v. (tail vein) injections of 3 mg/kg of the ADC, the unconjugated antibody, and vehicle alone. (A) Human lambda light chain concentrations correlating with tumor burden were determined by ELISA at the indicated time points and plotted as mean ± SD values. The p value (t-test) compares the ADC group to the unconjugated antibody group. (B) Kaplan-Meier survival curve. The p value (log-rank test) compares the ADC group to the unconjugated antibody group. See also Figure S3 and Table S3.

    Journal: Cell chemical biology

    Article Title: Stable and potent selenomab-drug conjugates

    doi: 10.1016/j.chembiol.2017.02.012

    Figure Lengend Snippet: Human multiple myeloma cell line U266-ffluc was i.v. (tail vein) injected into NSG mice. After 4 weeks, the mice were randomized into 3 groups comprising 8–9 mice each and treated with i.v. (tail vein) injections of 3 mg/kg of the ADC, the unconjugated antibody, and vehicle alone. (A) Human lambda light chain concentrations correlating with tumor burden were determined by ELISA at the indicated time points and plotted as mean ± SD values. The p value (t-test) compares the ADC group to the unconjugated antibody group. (B) Kaplan-Meier survival curve. The p value (log-rank test) compares the ADC group to the unconjugated antibody group. See also Figure S3 and Table S3.

    Article Snippet: Human Lambda ELISA Quantitation Set , Bethyl Laboratories , Cat#E80-116.

    Techniques: Injection, Enzyme-linked Immunosorbent Assay

    (A) Human breast cancer cell line KPL-4 was xenografted into the mammary fat pads of female NSG mice, grown to ~150 mm3, randomized into 5 groups comprising 6 mice each, and treated with i.v. (tail vein) injections of the indicated ADCs and controls four times every week at 5 mg/kg. Mean ± SD values are plotted. (B) Four female CD-1 mice were injected i.v. with 6 mg/kg of anti-HER2 scFv-Fc(Ser396Sec)/CN29. The plasma concentrations of total antibody and intact ADC were quantified by ELISA at the indicated time points, using HER2 ECD for capturing and either anti-His mAb (total antibody) or anti-MMAF mAb (intact ADC) for detection. Shown are mean ± SD values for each time point. See also Figure S5, Figure S6, Figure S7, and Table S6.

    Journal: Cell chemical biology

    Article Title: Stable and potent selenomab-drug conjugates

    doi: 10.1016/j.chembiol.2017.02.012

    Figure Lengend Snippet: (A) Human breast cancer cell line KPL-4 was xenografted into the mammary fat pads of female NSG mice, grown to ~150 mm3, randomized into 5 groups comprising 6 mice each, and treated with i.v. (tail vein) injections of the indicated ADCs and controls four times every week at 5 mg/kg. Mean ± SD values are plotted. (B) Four female CD-1 mice were injected i.v. with 6 mg/kg of anti-HER2 scFv-Fc(Ser396Sec)/CN29. The plasma concentrations of total antibody and intact ADC were quantified by ELISA at the indicated time points, using HER2 ECD for capturing and either anti-His mAb (total antibody) or anti-MMAF mAb (intact ADC) for detection. Shown are mean ± SD values for each time point. See also Figure S5, Figure S6, Figure S7, and Table S6.

    Article Snippet: Human Lambda ELISA Quantitation Set , Bethyl Laboratories , Cat#E80-116.

    Techniques: Injection, Clinical Proteomics, Enzyme-linked Immunosorbent Assay

    KEY RESOURCES TABLE

    Journal: Cell chemical biology

    Article Title: Stable and potent selenomab-drug conjugates

    doi: 10.1016/j.chembiol.2017.02.012

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Human Lambda ELISA Quantitation Set , Bethyl Laboratories , Cat#E80-116.

    Techniques: Virus, Recombinant, Marker, Conjugation Assay, Proliferation Assay, Enzyme-linked Immunosorbent Assay, Quantitation Assay, Software