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Charles River Laboratories retrogenix cell microarray
BNT351 shows comparable target affinity to 1-18 and no off-target binding (A) Left: the binding affinity of BNT351 and 1-18 to the stabilized HIV-1 envelope protein trimer (SOSIP BG505.664 ) by SPR (mean and individual values of triplicate measurements). Also see A. Right: binding of BNT351 and 1-18 to SOSIP BG505.664 by ELISA (mean ± standard deviation of triplicate measurements). (B) BNT351’s off-target binding was assessed by Retrogenix Cell <t>Microarray</t> Technology platform. Interactions identified during the library screen with ∼6,500 human proteins are shown. Also, see . Interaction with pepsinogen C (PGC) that appeared to be specific was re-tested by flow cytometry with HEK293 cells transfected with PGC. HEK293 cells transfected with FcγRIIIa and FcεRIg were used as a positive control. Duplicate measurements are shown for flow cytometry.
Retrogenix Cell Microarray, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Preclinical assessment of broadly neutralizing HIV-1 antibody BNT351 with optimized pharmacokinetics and potent antiviral activity"

Article Title: Preclinical assessment of broadly neutralizing HIV-1 antibody BNT351 with optimized pharmacokinetics and potent antiviral activity

Journal: iScience

doi: 10.1016/j.isci.2026.116022

BNT351 shows comparable target affinity to 1-18 and no off-target binding (A) Left: the binding affinity of BNT351 and 1-18 to the stabilized HIV-1 envelope protein trimer (SOSIP BG505.664 ) by SPR (mean and individual values of triplicate measurements). Also see A. Right: binding of BNT351 and 1-18 to SOSIP BG505.664 by ELISA (mean ± standard deviation of triplicate measurements). (B) BNT351’s off-target binding was assessed by Retrogenix Cell Microarray Technology platform. Interactions identified during the library screen with ∼6,500 human proteins are shown. Also, see . Interaction with pepsinogen C (PGC) that appeared to be specific was re-tested by flow cytometry with HEK293 cells transfected with PGC. HEK293 cells transfected with FcγRIIIa and FcεRIg were used as a positive control. Duplicate measurements are shown for flow cytometry.
Figure Legend Snippet: BNT351 shows comparable target affinity to 1-18 and no off-target binding (A) Left: the binding affinity of BNT351 and 1-18 to the stabilized HIV-1 envelope protein trimer (SOSIP BG505.664 ) by SPR (mean and individual values of triplicate measurements). Also see A. Right: binding of BNT351 and 1-18 to SOSIP BG505.664 by ELISA (mean ± standard deviation of triplicate measurements). (B) BNT351’s off-target binding was assessed by Retrogenix Cell Microarray Technology platform. Interactions identified during the library screen with ∼6,500 human proteins are shown. Also, see . Interaction with pepsinogen C (PGC) that appeared to be specific was re-tested by flow cytometry with HEK293 cells transfected with PGC. HEK293 cells transfected with FcγRIIIa and FcεRIg were used as a positive control. Duplicate measurements are shown for flow cytometry.

Techniques Used: Binding Assay, Enzyme-linked Immunosorbent Assay, Standard Deviation, Microarray, Flow Cytometry, Transfection, Positive Control

Related Articles

Microarray:

Article Title: Preclinical assessment of broadly neutralizing HIV-1 antibody BNT351 with optimized pharmacokinetics and potent antiviral activity
Article Snippet: .. The Retrogenix cell microarray was performed by Charles River Laboratories and included 6,105 full-length human proteins (plasma membrane proteins, secreted or a cell surface-tethered secreted proteins) and 400 human heterodimers. ..

Article Title: Characterization of JNJ-80948543 a novel CD79bxCD20xCD3 trispecific antibody for B-cell non-Hodgkin lymphoma.
Article Snippet: .. Each binding arm of JNJ-80948543 was evaluated for binding specificity in the Retrogenix® Cell Microarray Technology screen (Charles River Laboratories) and confirmed to be specific for the respective primary targets (data not shown). ..

Article Title: Preclinical assessment of broadly neutralizing HIV-1 antibody BNT351 with optimized pharmacokinetics and potent antiviral activity
Article Snippet: .. The Retrogenix cell microarray 30 was performed by Charles River Laboratories and included 6,105 full-length human proteins (plasma membrane proteins, secreted or a cell surface-tethered secreted proteins) and 400 human heterodimers. ..

Article Title: Characterization of JNJ-80948543 a novel CD79bxCD20xCD3 trispecific antibody for B-cell non-Hodgkin lymphoma.
Article Snippet: .. The individual CD79b, CD20 and CD3 molecules were screened for binding specificity using the Retrogenix® Cell Microarray Technology screen (Charles River Laboratories). ..

Article Title: CHS-114: an afucosylated anti-CCR8 monoclonal antibody that selectively depletes intratumoral Treg cells and induces antitumor immune responses
Article Snippet: .. Screening for off-target binding of anti-CCR8 antibodies Retrogenix cell microarray technology was used to screen for specific target binding. ..

Article Title: Ramantamig (JNJ-79635322), a novel T-cell-engaging trispecific antibody targeting BCMA, GPRC5D, and CD3, in multiple myeloma models
Article Snippet: For all in vivo studies, female nonobese diabetic (NOD) severe combined immunodeficiency (scid) gamma (NSG; The Jackson Laboratory) mice aged ∼5 to 8 weeks and weighing ∼20 g were used. .. The binding specificity of the individual BCMA and GPRC5D antibodies were assessed using the Retrogenix Cell Microarray Technology screen (Charles River Laboratories). ..

Clinical Proteomics:

Article Title: Preclinical assessment of broadly neutralizing HIV-1 antibody BNT351 with optimized pharmacokinetics and potent antiviral activity
Article Snippet: .. The Retrogenix cell microarray was performed by Charles River Laboratories and included 6,105 full-length human proteins (plasma membrane proteins, secreted or a cell surface-tethered secreted proteins) and 400 human heterodimers. ..

Article Title: Preclinical assessment of broadly neutralizing HIV-1 antibody BNT351 with optimized pharmacokinetics and potent antiviral activity
Article Snippet: .. The Retrogenix cell microarray 30 was performed by Charles River Laboratories and included 6,105 full-length human proteins (plasma membrane proteins, secreted or a cell surface-tethered secreted proteins) and 400 human heterodimers. ..

Membrane:

Article Title: Preclinical assessment of broadly neutralizing HIV-1 antibody BNT351 with optimized pharmacokinetics and potent antiviral activity
Article Snippet: .. The Retrogenix cell microarray was performed by Charles River Laboratories and included 6,105 full-length human proteins (plasma membrane proteins, secreted or a cell surface-tethered secreted proteins) and 400 human heterodimers. ..

Article Title: Preclinical assessment of broadly neutralizing HIV-1 antibody BNT351 with optimized pharmacokinetics and potent antiviral activity
Article Snippet: .. The Retrogenix cell microarray 30 was performed by Charles River Laboratories and included 6,105 full-length human proteins (plasma membrane proteins, secreted or a cell surface-tethered secreted proteins) and 400 human heterodimers. ..

Binding Assay:

Article Title: Characterization of JNJ-80948543 a novel CD79bxCD20xCD3 trispecific antibody for B-cell non-Hodgkin lymphoma.
Article Snippet: .. Each binding arm of JNJ-80948543 was evaluated for binding specificity in the Retrogenix® Cell Microarray Technology screen (Charles River Laboratories) and confirmed to be specific for the respective primary targets (data not shown). ..

Article Title: Overcoming claudin family homology: discovery of ARC101, a highly potent CLDN6-specific T-cell engager with a novel CD3 binder for ovarian adenocarcinoma
Article Snippet: MFI values were extracted, background-subtracted using secondary antibody control wells, and plotted in Prism (GraphPad, version 10.4) using a four-parameter nonlinear regression. .. Off-target binding interactions of ARC101 with human membrane and secreted proteins were assessed using Retrogenix® Cell Microarray Technology (Charles River Laboratories). ..

Article Title: Characterization of JNJ-80948543 a novel CD79bxCD20xCD3 trispecific antibody for B-cell non-Hodgkin lymphoma.
Article Snippet: .. The individual CD79b, CD20 and CD3 molecules were screened for binding specificity using the Retrogenix® Cell Microarray Technology screen (Charles River Laboratories). ..

Article Title: CHS-114: an afucosylated anti-CCR8 monoclonal antibody that selectively depletes intratumoral Treg cells and induces antitumor immune responses
Article Snippet: .. Importantly, as specificity can often be challenging for antibodies against GPCRs, a cell-based proteome array screen (Retrogenix) demonstrated that CHS-114 bound exclusively to CCR8 and exhibited no discernable off-target binding to extracellular or secreted proteins (Fig. 3B). ..

Article Title: CHS-114: an afucosylated anti-CCR8 monoclonal antibody that selectively depletes intratumoral Treg cells and induces antitumor immune responses
Article Snippet: .. Screening for off-target binding of anti-CCR8 antibodies Retrogenix cell microarray technology was used to screen for specific target binding. ..

Article Title: Ramantamig (JNJ-79635322), a novel T-cell-engaging trispecific antibody targeting BCMA, GPRC5D, and CD3, in multiple myeloma models
Article Snippet: For all in vivo studies, female nonobese diabetic (NOD) severe combined immunodeficiency (scid) gamma (NSG; The Jackson Laboratory) mice aged ∼5 to 8 weeks and weighing ∼20 g were used. .. The binding specificity of the individual BCMA and GPRC5D antibodies were assessed using the Retrogenix Cell Microarray Technology screen (Charles River Laboratories). ..



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Charles River Laboratories retrogenix cell microarray
BNT351 shows comparable target affinity to 1-18 and no off-target binding (A) Left: the binding affinity of BNT351 and 1-18 to the stabilized HIV-1 envelope protein trimer (SOSIP BG505.664 ) by SPR (mean and individual values of triplicate measurements). Also see A. Right: binding of BNT351 and 1-18 to SOSIP BG505.664 by ELISA (mean ± standard deviation of triplicate measurements). (B) BNT351’s off-target binding was assessed by Retrogenix Cell <t>Microarray</t> Technology platform. Interactions identified during the library screen with ∼6,500 human proteins are shown. Also, see . Interaction with pepsinogen C (PGC) that appeared to be specific was re-tested by flow cytometry with HEK293 cells transfected with PGC. HEK293 cells transfected with FcγRIIIa and FcεRIg were used as a positive control. Duplicate measurements are shown for flow cytometry.
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BNT351 shows comparable target affinity to 1-18 and no off-target binding (A) Left: the binding affinity of BNT351 and 1-18 to the stabilized HIV-1 envelope protein trimer (SOSIP BG505.664 ) by SPR (mean and individual values of triplicate measurements). Also see A. Right: binding of BNT351 and 1-18 to SOSIP BG505.664 by ELISA (mean ± standard deviation of triplicate measurements). (B) BNT351’s off-target binding was assessed by Retrogenix Cell <t>Microarray</t> Technology platform. Interactions identified during the library screen with ∼6,500 human proteins are shown. Also, see . Interaction with pepsinogen C (PGC) that appeared to be specific was re-tested by flow cytometry with HEK293 cells transfected with PGC. HEK293 cells transfected with FcγRIIIa and FcεRIg were used as a positive control. Duplicate measurements are shown for flow cytometry.
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BNT351 shows comparable target affinity to 1-18 and no off-target binding (A) Left: the binding affinity of BNT351 and 1-18 to the stabilized HIV-1 envelope protein trimer (SOSIP BG505.664 ) by SPR (mean and individual values of triplicate measurements). Also see A. Right: binding of BNT351 and 1-18 to SOSIP BG505.664 by ELISA (mean ± standard deviation of triplicate measurements). (B) BNT351’s off-target binding was assessed by Retrogenix Cell <t>Microarray</t> Technology platform. Interactions identified during the library screen with ∼6,500 human proteins are shown. Also, see . Interaction with pepsinogen C (PGC) that appeared to be specific was re-tested by flow cytometry with HEK293 cells transfected with PGC. HEK293 cells transfected with FcγRIIIa and FcεRIg were used as a positive control. Duplicate measurements are shown for flow cytometry.
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Image Search Results


BNT351 shows comparable target affinity to 1-18 and no off-target binding (A) Left: the binding affinity of BNT351 and 1-18 to the stabilized HIV-1 envelope protein trimer (SOSIP BG505.664 ) by SPR (mean and individual values of triplicate measurements). Also see A. Right: binding of BNT351 and 1-18 to SOSIP BG505.664 by ELISA (mean ± standard deviation of triplicate measurements). (B) BNT351’s off-target binding was assessed by Retrogenix Cell Microarray Technology platform. Interactions identified during the library screen with ∼6,500 human proteins are shown. Also, see . Interaction with pepsinogen C (PGC) that appeared to be specific was re-tested by flow cytometry with HEK293 cells transfected with PGC. HEK293 cells transfected with FcγRIIIa and FcεRIg were used as a positive control. Duplicate measurements are shown for flow cytometry.

Journal: iScience

Article Title: Preclinical assessment of broadly neutralizing HIV-1 antibody BNT351 with optimized pharmacokinetics and potent antiviral activity

doi: 10.1016/j.isci.2026.116022

Figure Lengend Snippet: BNT351 shows comparable target affinity to 1-18 and no off-target binding (A) Left: the binding affinity of BNT351 and 1-18 to the stabilized HIV-1 envelope protein trimer (SOSIP BG505.664 ) by SPR (mean and individual values of triplicate measurements). Also see A. Right: binding of BNT351 and 1-18 to SOSIP BG505.664 by ELISA (mean ± standard deviation of triplicate measurements). (B) BNT351’s off-target binding was assessed by Retrogenix Cell Microarray Technology platform. Interactions identified during the library screen with ∼6,500 human proteins are shown. Also, see . Interaction with pepsinogen C (PGC) that appeared to be specific was re-tested by flow cytometry with HEK293 cells transfected with PGC. HEK293 cells transfected with FcγRIIIa and FcεRIg were used as a positive control. Duplicate measurements are shown for flow cytometry.

Article Snippet: The Retrogenix cell microarray was performed by Charles River Laboratories and included 6,105 full-length human proteins (plasma membrane proteins, secreted or a cell surface-tethered secreted proteins) and 400 human heterodimers.

Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Standard Deviation, Microarray, Flow Cytometry, Transfection, Positive Control