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strain 9320  (ATCC)


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    Structured Review

    ATCC strain 9320
    Strain 9320, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/9320s/Human+respiratory+syncytial+virus/us12372525-385-8-10
    Average 95 stars, based on 39 article reviews
    strain 9320 - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Infection:

    Article Title: ZFP91 restricts RSV replication by driving K48-linked ubiquitination and proteasomal degradation of M2-1.
    Article Snippet: .. When cell confluence reached 70%–90%, cells were infected with mCherry-labeled RSV subtype A (L19 strain) at a multiplicity of infection (MOI) of 1 in DMEM supplemented with 2% serum for 2 h, with gentle agitation every 15 min. For subtype validation experiments, RSV subtype B (ATCC VR-955) was used to infect Hep2 cells under the same conditions. ..

    Gentle:

    Article Title: ZFP91 restricts RSV replication by driving K48-linked ubiquitination and proteasomal degradation of M2-1.
    Article Snippet: .. When cell confluence reached 70%–90%, cells were infected with mCherry-labeled RSV subtype A (L19 strain) at a multiplicity of infection (MOI) of 1 in DMEM supplemented with 2% serum for 2 h, with gentle agitation every 15 min. For subtype validation experiments, RSV subtype B (ATCC VR-955) was used to infect Hep2 cells under the same conditions. ..

    Biomarker Discovery:

    Article Title: ZFP91 restricts RSV replication by driving K48-linked ubiquitination and proteasomal degradation of M2-1.
    Article Snippet: .. When cell confluence reached 70%–90%, cells were infected with mCherry-labeled RSV subtype A (L19 strain) at a multiplicity of infection (MOI) of 1 in DMEM supplemented with 2% serum for 2 h, with gentle agitation every 15 min. For subtype validation experiments, RSV subtype B (ATCC VR-955) was used to infect Hep2 cells under the same conditions. ..

    other:

    Article Title: Reliability of RSV Antigen Diagnostic Tests in the Presence of Nirsevimab
    Article Snippet: RSV A2 (ATCC VR-1540) and B9320 (ATCC VR-955) were purchased from American Type Culture Collection (Manassas, VA) and propagated before use in Hep-2 (ATCC CCL-23) or Vero cells (ATCC CCL-81), respectively.

    Article Title: Rhein inhibits respiratory syncytial virus entry by stabilizing the prefusion conformation of the fusion protein.
    Article Snippet: Two representative RSV subtype B strains (9320 (ATCC VR-955) and 9393) were used.

    Article Title: Rhein inhibits respiratory syncytial virus entry by stabilizing the prefusion conformation of the fusion protein.
    Article Snippet: RSV strains, including subtype A (A2 strain) and subtype B (9320 and 9393 strains), were obtained from the Virus Institute of Wuhan University (Wuhan, China).

    Concentration Assay:

    Article Title:
    Article Snippet: .. Type Strain Source Concentration Detected Unit B WV/14617/85 ATCC VR-1400 4.07E+00 TCID50/mL 9320 ATCC VR-955 4.07E+00 TCID50/mL Strain Source Concentration Detected Unit TW-183 ATCC VR-2282 6.43E-02 TCID50/mL AR-39 ATCC 53592 3.09E+00 TCID50/mL TWAR (CDC/CWL-029) ATCC VR-1310 1.24E+01 TCID50/mL TWAR strain 2043 ATCC VR-1355 7.72E-01 TCID50/mL TWAR strain 2023 ATCC VR-1356 1.93E-01 TCID50/mL CM-1 ATCC VR-1360 1.93E-01 TCID50/mL J-21 ATCC VR-1435 7.72E-01 TCID50/mL AO3 ATCC VR-1452 1.93E-01 TCID50/mL Laboratory testing was supplemented with in silico data where prediction rules were used to predict reactivity and cross‐reactivity of specific Influenza A strains. ..

    In Silico:

    Article Title:
    Article Snippet: .. Type Strain Source Concentration Detected Unit B WV/14617/85 ATCC VR-1400 4.07E+00 TCID50/mL 9320 ATCC VR-955 4.07E+00 TCID50/mL Strain Source Concentration Detected Unit TW-183 ATCC VR-2282 6.43E-02 TCID50/mL AR-39 ATCC 53592 3.09E+00 TCID50/mL TWAR (CDC/CWL-029) ATCC VR-1310 1.24E+01 TCID50/mL TWAR strain 2043 ATCC VR-1355 7.72E-01 TCID50/mL TWAR strain 2023 ATCC VR-1356 1.93E-01 TCID50/mL CM-1 ATCC VR-1360 1.93E-01 TCID50/mL J-21 ATCC VR-1435 7.72E-01 TCID50/mL AO3 ATCC VR-1452 1.93E-01 TCID50/mL Laboratory testing was supplemented with in silico data where prediction rules were used to predict reactivity and cross‐reactivity of specific Influenza A strains. ..

    Virus:

    Article Title: N4-hydroxycytidine and derivatives and anti-viral uses related thereto
    Article Snippet: Virus Preparation—The RSV strain Long and RSV strain 9320 were obtained from ATCC (catalog #VR-26 and catalog #VR-955, respectively) and were grown in HEp2 cells for the production of stock virus pools. .. Virus Preparation—The RSV strain Long and RSV strain 9320 were obtained from ATCC (catalog #VR-26 and catalog #VR-955, respectively) and were grown in HEp2 cells for the production of stock virus pools. ..



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    Description of the evolution experiment workflow and model plasmid properties. (a) The novel kanamycin resistance allele ( nptII ) was introduced into a homoplasmic population of plasmid hosts where it integrates into a single plasmid copy in a subset of cells. The donor population was evolved by daily serial transfers; agitation prevents conjugation within the donor culture (Table S5). Cell type frequencies are quantified by ddPCR. Donors are mated daily with a plasmid-free recipient population on solid agar. Recipient genotypes are determined by differential plating. This design allows separate observation of conjugation and segregational drift. Plasmids copy number is supplied in the table below as the mean ±95% CI. The presence of partitioning system (Par) and toxin-antitoxin system (TA) is indicated. (b) Model plasmid stability in the Acinetobacter baylyi <t>BD413</t> host over 7 days (transfers). Error bars correspond to standard deviation. (c) Relative fitness w of homoplasmic ancestral hosts compared with plasmid-free hosts (with the following sample sizes: n pNCL =8; n pCLR =6; n pCHR =8) (left), homoplasmic novel allele host compared to plasmid-free cells (n pNCL =6; n pCLR =5; n pCHR =7) (middle), and homoplasmic ancestral allele hosts compared with homoplasmic novel allele hosts (n pNCL =7; n pCLR =5; n pCHR =8) (right). Error bars correspond to 95% CI; time series data of the measurements, linear regression analysis and relative fitness values are supplied in Fig. S1. d) Allele frequency as quantified by the established ddPCR protocol was validated by plating. Error bars correspond to 95% CI.
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    Description of the evolution experiment workflow and model plasmid properties. (a) The novel kanamycin resistance allele ( nptII ) was introduced into a homoplasmic population of plasmid hosts where it integrates into a single plasmid copy in a subset of cells. The donor population was evolved by daily serial transfers; agitation prevents conjugation within the donor culture (Table S5). Cell type frequencies are quantified by ddPCR. Donors are mated daily with a plasmid-free recipient population on solid agar. Recipient genotypes are determined by differential plating. This design allows separate observation of conjugation and segregational drift. Plasmids copy number is supplied in the table below as the mean ±95% CI. The presence of partitioning system (Par) and toxin-antitoxin system (TA) is indicated. (b) Model plasmid stability in the Acinetobacter baylyi <t>BD413</t> host over 7 days (transfers). Error bars correspond to standard deviation. (c) Relative fitness w of homoplasmic ancestral hosts compared with plasmid-free hosts (with the following sample sizes: n pNCL =8; n pCLR =6; n pCHR =8) (left), homoplasmic novel allele host compared to plasmid-free cells (n pNCL =6; n pCLR =5; n pCHR =7) (middle), and homoplasmic ancestral allele hosts compared with homoplasmic novel allele hosts (n pNCL =7; n pCLR =5; n pCHR =8) (right). Error bars correspond to 95% CI; time series data of the measurements, linear regression analysis and relative fitness values are supplied in Fig. S1. d) Allele frequency as quantified by the established ddPCR protocol was validated by plating. Error bars correspond to 95% CI.
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    Description of the evolution experiment workflow and model plasmid properties. (a) The novel kanamycin resistance allele ( nptII ) was introduced into a homoplasmic population of plasmid hosts where it integrates into a single plasmid copy in a subset of cells. The donor population was evolved by daily serial transfers; agitation prevents conjugation within the donor culture (Table S5). Cell type frequencies are quantified by ddPCR. Donors are mated daily with a plasmid-free recipient population on solid agar. Recipient genotypes are determined by differential plating. This design allows separate observation of conjugation and segregational drift. Plasmids copy number is supplied in the table below as the mean ±95% CI. The presence of partitioning system (Par) and toxin-antitoxin system (TA) is indicated. (b) Model plasmid stability in the Acinetobacter baylyi <t>BD413</t> host over 7 days (transfers). Error bars correspond to standard deviation. (c) Relative fitness w of homoplasmic ancestral hosts compared with plasmid-free hosts (with the following sample sizes: n pNCL =8; n pCLR =6; n pCHR =8) (left), homoplasmic novel allele host compared to plasmid-free cells (n pNCL =6; n pCLR =5; n pCHR =7) (middle), and homoplasmic ancestral allele hosts compared with homoplasmic novel allele hosts (n pNCL =7; n pCLR =5; n pCHR =8) (right). Error bars correspond to 95% CI; time series data of the measurements, linear regression analysis and relative fitness values are supplied in Fig. S1. d) Allele frequency as quantified by the established ddPCR protocol was validated by plating. Error bars correspond to 95% CI.
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    strain 9320  (ATCC)
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    Description of the evolution experiment workflow and model plasmid properties. (a) The novel kanamycin resistance allele ( nptII ) was introduced into a homoplasmic population of plasmid hosts where it integrates into a single plasmid copy in a subset of cells. The donor population was evolved by daily serial transfers; agitation prevents conjugation within the donor culture (Table S5). Cell type frequencies are quantified by ddPCR. Donors are mated daily with a plasmid-free recipient population on solid agar. Recipient genotypes are determined by differential plating. This design allows separate observation of conjugation and segregational drift. Plasmids copy number is supplied in the table below as the mean ±95% CI. The presence of partitioning system (Par) and toxin-antitoxin system (TA) is indicated. (b) Model plasmid stability in the Acinetobacter baylyi <t>BD413</t> host over 7 days (transfers). Error bars correspond to standard deviation. (c) Relative fitness w of homoplasmic ancestral hosts compared with plasmid-free hosts (with the following sample sizes: n pNCL =8; n pCLR =6; n pCHR =8) (left), homoplasmic novel allele host compared to plasmid-free cells (n pNCL =6; n pCLR =5; n pCHR =7) (middle), and homoplasmic ancestral allele hosts compared with homoplasmic novel allele hosts (n pNCL =7; n pCLR =5; n pCHR =8) (right). Error bars correspond to 95% CI; time series data of the measurements, linear regression analysis and relative fitness values are supplied in Fig. S1. d) Allele frequency as quantified by the established ddPCR protocol was validated by plating. Error bars correspond to 95% CI.
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    acinetobacter sp  (DSMZ)
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    Description of the evolution experiment workflow and model plasmid properties. (a) The novel kanamycin resistance allele ( nptII ) was introduced into a homoplasmic population of plasmid hosts where it integrates into a single plasmid copy in a subset of cells. The donor population was evolved by daily serial transfers; agitation prevents conjugation within the donor culture (Table S5). Cell type frequencies are quantified by ddPCR. Donors are mated daily with a plasmid-free recipient population on solid agar. Recipient genotypes are determined by differential plating. This design allows separate observation of conjugation and segregational drift. Plasmids copy number is supplied in the table below as the mean ±95% CI. The presence of partitioning system (Par) and toxin-antitoxin system (TA) is indicated. (b) Model plasmid stability in the Acinetobacter baylyi <t>BD413</t> host over 7 days (transfers). Error bars correspond to standard deviation. (c) Relative fitness w of homoplasmic ancestral hosts compared with plasmid-free hosts (with the following sample sizes: n pNCL =8; n pCLR =6; n pCHR =8) (left), homoplasmic novel allele host compared to plasmid-free cells (n pNCL =6; n pCLR =5; n pCHR =7) (middle), and homoplasmic ancestral allele hosts compared with homoplasmic novel allele hosts (n pNCL =7; n pCLR =5; n pCHR =8) (right). Error bars correspond to 95% CI; time series data of the measurements, linear regression analysis and relative fitness values are supplied in Fig. S1. d) Allele frequency as quantified by the established ddPCR protocol was validated by plating. Error bars correspond to 95% CI.
    Acinetobacter Sp, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Description of the evolution experiment workflow and model plasmid properties. (a) The novel kanamycin resistance allele ( nptII ) was introduced into a homoplasmic population of plasmid hosts where it integrates into a single plasmid copy in a subset of cells. The donor population was evolved by daily serial transfers; agitation prevents conjugation within the donor culture (Table S5). Cell type frequencies are quantified by ddPCR. Donors are mated daily with a plasmid-free recipient population on solid agar. Recipient genotypes are determined by differential plating. This design allows separate observation of conjugation and segregational drift. Plasmids copy number is supplied in the table below as the mean ±95% CI. The presence of partitioning system (Par) and toxin-antitoxin system (TA) is indicated. (b) Model plasmid stability in the Acinetobacter baylyi BD413 host over 7 days (transfers). Error bars correspond to standard deviation. (c) Relative fitness w of homoplasmic ancestral hosts compared with plasmid-free hosts (with the following sample sizes: n pNCL =8; n pCLR =6; n pCHR =8) (left), homoplasmic novel allele host compared to plasmid-free cells (n pNCL =6; n pCLR =5; n pCHR =7) (middle), and homoplasmic ancestral allele hosts compared with homoplasmic novel allele hosts (n pNCL =7; n pCLR =5; n pCHR =8) (right). Error bars correspond to 95% CI; time series data of the measurements, linear regression analysis and relative fitness values are supplied in Fig. S1. d) Allele frequency as quantified by the established ddPCR protocol was validated by plating. Error bars correspond to 95% CI.

    Journal: bioRxiv

    Article Title: Horizontal transfer promotes allele segregation in multicopy plasmids

    doi: 10.64898/2026.03.13.711586

    Figure Lengend Snippet: Description of the evolution experiment workflow and model plasmid properties. (a) The novel kanamycin resistance allele ( nptII ) was introduced into a homoplasmic population of plasmid hosts where it integrates into a single plasmid copy in a subset of cells. The donor population was evolved by daily serial transfers; agitation prevents conjugation within the donor culture (Table S5). Cell type frequencies are quantified by ddPCR. Donors are mated daily with a plasmid-free recipient population on solid agar. Recipient genotypes are determined by differential plating. This design allows separate observation of conjugation and segregational drift. Plasmids copy number is supplied in the table below as the mean ±95% CI. The presence of partitioning system (Par) and toxin-antitoxin system (TA) is indicated. (b) Model plasmid stability in the Acinetobacter baylyi BD413 host over 7 days (transfers). Error bars correspond to standard deviation. (c) Relative fitness w of homoplasmic ancestral hosts compared with plasmid-free hosts (with the following sample sizes: n pNCL =8; n pCLR =6; n pCHR =8) (left), homoplasmic novel allele host compared to plasmid-free cells (n pNCL =6; n pCLR =5; n pCHR =7) (middle), and homoplasmic ancestral allele hosts compared with homoplasmic novel allele hosts (n pNCL =7; n pCLR =5; n pCHR =8) (right). Error bars correspond to 95% CI; time series data of the measurements, linear regression analysis and relative fitness values are supplied in Fig. S1. d) Allele frequency as quantified by the established ddPCR protocol was validated by plating. Error bars correspond to 95% CI.

    Article Snippet: All experiments were performed using the model strain Acinetobacter baylyi BD413 and BD4 (DSM 588 and DSM 586; German Collection of Microorganisms and Cell Cultures, DSMZ).

    Techniques: Plasmid Preparation, Conjugation Assay, Standard Deviation