5x sds loading buffer (Applygen Technologies)

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Applygen Technologies 5x sds loading buffer
5x Sds Loading Buffer, supplied by Applygen Technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5x+sds+loading+buffer/buffer+loading/pmc12768472-107-6-44
Average 86 stars, based on 1 article reviews

5x sds loading buffer - by Bioz Stars, 2026-09

86/100 stars

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SDS Page:

Article Title: Forsythiaside A Alleviates Kidney Injury and Intestinal Epithelium Dysfunction in IgA Nephropathy by Inhibiting TLR4/NF-κB Signaling.
Article Snippet: The lysates were then centrifuged at 12000×g for 20 min at 4°C and the protein concentration in the resulting supernatant was measured using a Bradford protein assay kit (#P1510; APPLYGEN). .. Then, 20 μg of protein from the samples were mixed with SDS- PAGE loading buffer (#B1007; APPLYGEN), heated for 5 min at 95°C, separated on SDS- PAGE, and transferred to polyvinylidene fluoride (PVDF) membranes (0.45 μm). ..

Article Title: ICAM1-Mediated Endothelial Cells and Macrophage Interactions in Modulating GBM Malignant Transformation
Article Snippet: .. For Western blotting, radioimmunoprecipitation assay (RIPA) buffer (R0010, Solarbio) was used to prepare whole-cell lysates that were mixed with 5×sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) loading buffer (B1012, Applygen) and separated by 10% SDS-PAGE (PG112, EpiZyme). .. Proteins were transferred to a polyvinylidene fluoride (PVDF) membrane (EQ00010, Millipore) and blocked with 5% non-fat milk (B1009, Applygen) in Tris-Borate-Sodium Tween-20 (TBST).

Article Title: CDK5-Induced HCN2 Channel Dysfunction in the Prelimbic Cortex Drives Allodynia and Anxiety-Like Behaviors in Neuropathic Pain.
Article Snippet: The prelimbic cortex (PL) plays a critical role in processing both the sensory and affective components of pain.. However, the underlying molecular mechanisms remain poorly understood.. In this study, we observed a reduction in hyperpolarization-activated cation current (Ih) in layer V pyramidal neurons of the contralateral PL in a mouse model of spared nerve injury (SNI).

Article Title: The crosstalk between chaperone-mediated autophagy and apoptosis via ATM/p53-mitochondria-dependent signaling contributed to ischemia-induced cerebral injuries.
Article Snippet: Stroke is the second leading cause of death and a leading cause of disability worldwide.. Neuronal loss is a significant factor in determining the outcome of ischemic stroke.. However, there is no effective treatment for neuronal loss caused by stroke.

Western Blot:

Article Title: TRIM16 attenuates TDP43-mediated oxidative injury by coordinating Nrf2 activation and TFR1 autophagic degradation.
Article Snippet: TAR DNA-binding protein 43 (TDP43) aggregation is a well-established pathological hallmark of amyotrophic lateral sclerosis (ALS) and related neurodegenerative disorders, contributing significantly to oxidative stress and neuronal injury.. Here, we report that the M337V mutation in TDP43 exacerbates its proteotoxicity relative to the wild-type protein.. Concurrently, multi-omics analysis revealed a pronounced downregulation of TRIM16 in motor neuron-like cells expressing either wild-type or M337V mutant TDP43.

Concentration Assay:

Article Title: Lactobacillus plantarum RG-034 colonic soluble capsules alleviate functional constipation in rats by promoting short-chain fatty acid generation.
Article Snippet: .. The protein was diluted to an equal concentration with PBS and boiled with 5× loading buffer (B1012, Applygen) for 10 min. Proteins (50 μg) were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to a polyvinylidene difluoride (PVDF) membrane (IPVH00010, Millipore). .. After blocking with 5% degrease milk for 2 h, the membranes were incubated overnight at 4 °C with the following primary antibody: anti-PI3K (1 : 2000, A4992, Abclonal), anti-AKT (1 : 4000, ab179463, Abcam), anti-p-PI3K (1 : 1000, AP0854, Abclonal), anti-p-AKT (1 : 4000, ab81283, Abcam) and anti-GAPDH (1 : 4000, AF0721, Affinity).

Nucleic Acid Electrophoresis:

Membrane:

Radio Immunoprecipitation:

Polyacrylamide Gel Electrophoresis:

Article Title: Selective autophagic degradation of glycolytic activator PFKFB3 contributes to maintaining intestinal epithelial barrier in inflammatory bowel disease
Article Snippet: .. The protein was denatured by adding 5X SDS loading buffer and boiled at 100°C for 10 min, then separated by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE). β-actin antibody and the target protein antibody were diluted separately in primary antibody dilution buffer (cat no. C1240; Applygen) at 1:1,000, followed by overnight incubation at 4°C. .. On the following day, the goat anti-rabbit IgG secondary antibody (cat no. BA1056; Wuhan Boster Biological Technology, Ltd.) was diluted in secondary antibody diluent buffer (cat no. C1241; Applygen) at 1:5,000 and incubated at 37°C for 1 h before being washed three times with PBST buffer, 5 min per wash.

Incubation:

Protease Inhibitor:

Article Title: Deoxypodophyllotoxin inhibits lung adenocarcinoma growth through regulation of FOXO1 nuclear translocation.
Article Snippet: Lung adenocarcinoma (LUAD) is the most common histological subtype of lung cancer and remains associated with high mortality despite advances in current therapies.. Deoxypodophyllotoxin (DPT), a natural lignan derived from Anthriscus sylvestris, has been reported to exhibit antitumor activity in multiple cancer types; however, its molecular mechanism of action in LUAD has not been fully elucidated.. In this study, we investigated the antitumor effects of DPT in LUAD and explored the involvement of Forkhead box O1 (FOXO1) signaling.

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