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3d cell explorer-fluo holotomographic microscope  (NanoLive Inc)

 
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    NanoLive Inc 3d cell explorer-fluo holotomographic microscope
    <t>Holotomographic</t> images of patient-derived CAF morphology in vitro. ( A ) An example of adherent–fibrotic, stellate-shaped morphology. ( B ) Intermediate CAFs with elongated and star-like cell morphology. ( C ) Invasive–migratory spindle-shaped morphology. Brighter regions correspond to areas of higher dry mass density. A 90 × 90 µm field of view is shown.
    3d Cell Explorer Fluo Holotomographic Microscope, supplied by NanoLive Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3d+interactive+microscopy+visualization+software/live+cell+three+dimensional+holotomographic+microscopy+3d+cell+explorer/pmc11852712-78-12-17
    Average 90 stars, based on 1 article reviews
    3d cell explorer-fluo holotomographic microscope - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Patient-Derived Cancer-Associated Fibroblasts Support the Colonization of Tumor Cells in Head and Neck Squamous Cell Carcinoma"

    Article Title: Patient-Derived Cancer-Associated Fibroblasts Support the Colonization of Tumor Cells in Head and Neck Squamous Cell Carcinoma

    Journal: Biomedicines

    doi: 10.3390/biomedicines13020358

    Holotomographic images of patient-derived CAF morphology in vitro. ( A ) An example of adherent–fibrotic, stellate-shaped morphology. ( B ) Intermediate CAFs with elongated and star-like cell morphology. ( C ) Invasive–migratory spindle-shaped morphology. Brighter regions correspond to areas of higher dry mass density. A 90 × 90 µm field of view is shown.
    Figure Legend Snippet: Holotomographic images of patient-derived CAF morphology in vitro. ( A ) An example of adherent–fibrotic, stellate-shaped morphology. ( B ) Intermediate CAFs with elongated and star-like cell morphology. ( C ) Invasive–migratory spindle-shaped morphology. Brighter regions correspond to areas of higher dry mass density. A 90 × 90 µm field of view is shown.

    Techniques Used: Derivative Assay, In Vitro

    Holotomographic imaging of tumor cells with direct physical contact with CAFs. ( A ) Tumor cells containing lipid droplets (bright white dots) and vacuoles of different sizes (black circles). Fine adhesions can be seen attaching cells to the plate surface. ( B ) An example of a tumor cell with increased surface area, the appearance of stress fibers (straight-line structures), and further membrane disruption. ( C ) Tumor cells containing small vacuoles, seen as black circles (left). The disruption of tumor cell plasma membranes is also visible as brighter areas around the tumor cell perimeter. Brighter regions correspond to areas of higher dry mass density. A 90 × 90 µm field of view is shown.
    Figure Legend Snippet: Holotomographic imaging of tumor cells with direct physical contact with CAFs. ( A ) Tumor cells containing lipid droplets (bright white dots) and vacuoles of different sizes (black circles). Fine adhesions can be seen attaching cells to the plate surface. ( B ) An example of a tumor cell with increased surface area, the appearance of stress fibers (straight-line structures), and further membrane disruption. ( C ) Tumor cells containing small vacuoles, seen as black circles (left). The disruption of tumor cell plasma membranes is also visible as brighter areas around the tumor cell perimeter. Brighter regions correspond to areas of higher dry mass density. A 90 × 90 µm field of view is shown.

    Techniques Used: Imaging, Membrane, Disruption, Clinical Proteomics

    Related Articles

    other:

    Article Title: Efflux pump inhibitors from plant compounds: A new strategy to modulate bacterial growth and morphology
    Article Snippet: Measurements were performed with the use of the DHT system (3D Cell Explorer, Nanolive, Ecublens, Switzerland) with using top-stage incubator (Okolab for Nanolive, Pozzuoli, Italy) and the spatial distribution of the refractive index (RI) was obtained as the measurement result.

    Article Title: Patient-Derived Cancer-Associated Fibroblasts Support the Colonization of Tumor Cells in Head and Neck Squamous Cell Carcinoma
    Article Snippet: Next, the cells were analyzed for their morphology and interaction under a 3D Cell Explorer-fluo holotomographic microscope (Nanolive SA, Tolochenaz, Switzerland) with an air objective at 60× magnification.

    Article Title: GPR56/ADGRG1 induces biased Rho-ROCK-MLC and JAK-STAT3 signaling to promote amoeboid-like morphology and IL-6 upregulation in melanoma cells
    Article Snippet: Cell morphologies were observed and recorded using Holo-Tomography 3D Cell Explorer (NanoLive, Switzerland).

    Article Title: Patient-Derived Cancer-Associated Fibroblasts Support the Colonization of Tumor Cells in Head and Neck Squamous Cell Carcinoma
    Article Snippet: Next, the cells were analyzed for their morphology and interaction under a 3D Cell Explorer-fluo holotomographic microscope (Nanolive SA, Tolochenaz, Switzerland) with an air objective at 60×magnification.

    Microscopy:

    Article Title: Immune evasion through mitochondrial transfer in the tumour microenvironment.
    Article Snippet: .. Twenty-four hours later, we began capturing images every 30 min using a digital holographic microscope (3D Cell Explorer CX-A, Nanolive). .. The images were analysed using Fiji software (https://imagej.net/ software/fiji).

    Article Title: Immune evasion through mitochondrial transfer in the tumour microenvironment
    Article Snippet: .. Twenty-four hours later, we began capturing images every 30 min using a digital holographic microscope (3D Cell Explorer CX-A, Nanolive). .. The images were analysed using Fiji software ( https://imagej.net/software/fiji ).

    Article Title: Type-dependent effects of nanoplastics on microglial activation and CXCR2-mediated chemotactic migration.
    Article Snippet: To analyze the uptake and internalization patterns of the three different nanoplastics in microglia, cells were subcultured in tissue culture dishes (FluoroDishTM, WPI Inc., USA). .. After 3 hours of exposure to 50 μg mL−1 concentration of each nanoplastic, the cells were analyzed using a holotomographic microscope equipped with a fluorescence module (3D Cell ExplorerFluo, Nanolive, Lausanne, Switzerland). ..

    Concentration Assay:

    Article Title: Type-dependent effects of nanoplastics on microglial activation and CXCR2-mediated chemotactic migration.
    Article Snippet: To analyze the uptake and internalization patterns of the three different nanoplastics in microglia, cells were subcultured in tissue culture dishes (FluoroDishTM, WPI Inc., USA). .. After 3 hours of exposure to 50 μg mL−1 concentration of each nanoplastic, the cells were analyzed using a holotomographic microscope equipped with a fluorescence module (3D Cell ExplorerFluo, Nanolive, Lausanne, Switzerland). ..

    Fluorescence:

    Article Title: Type-dependent effects of nanoplastics on microglial activation and CXCR2-mediated chemotactic migration.
    Article Snippet: To analyze the uptake and internalization patterns of the three different nanoplastics in microglia, cells were subcultured in tissue culture dishes (FluoroDishTM, WPI Inc., USA). .. After 3 hours of exposure to 50 μg mL−1 concentration of each nanoplastic, the cells were analyzed using a holotomographic microscope equipped with a fluorescence module (3D Cell ExplorerFluo, Nanolive, Lausanne, Switzerland). ..



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    ( A ) <t>3D</t> surface projection of IHCs and OHCs from the cochlear 32 kHz region of Clic5 +/− , Clic5 −/− and Clic5 −/− injected with scAAV. Clic5 at 12 weeks, stained with a CLIC5 (red) and actin (gray). ( B ) Representative confocal images of IHCs from the apical turn of Clic5 +/− and Clic5 −/− injected with scAAV. Clic5 at 12 weeks, stained with a CLIC5 (red), actin (gray), and FLAG tag (green). ( C ) Representative confocal images of IHCs and OHCs from the cochlear 8 kHz region of Clic5 +/− , Clic5 −/− , and Clic5 −/− injected with scAAV. Clic5 at 12 weeks, stained with actin. ( D ) Stereocilia length measurements of IHC at different regions of the cochlea, n = 3 for Clic5 +/− , Clic5 −/− , and Clic5 −/− injected with scAAV. Clic5 . ( E ) Quantification of OHC survival at different regions of the cochlea, n = 3 for Clic5 +/− , Clic5 −/− , and Clic5 −/− injected with scAAV. Clic5 . ( F ) Quantification of IHC survival at different regions of the cochlea, n = 3 for Clic5 +/− , Clic5 −/− , and Clic5 −/− injected with scAAV. Clic5 . ( G ) High-magnification scanning electron <t>microscopy</t> images of outer and inner hair bundles from the cochlea middle-turn of the Clic5 −/− and Clic5 −/− injected with scAAV. Clic5 at 12 weeks. ( H ) Representative images of IHCs and OHCs at 12 weeks at different regions of the cochlea of Clic5 −/ − injected with scAAV. Clic5 . ( I ) ABR thresholds at 4 weeks of Clic5 +/− ( n = 11), Clic5 −/− ( n = 10), and Clic5 −/− injected with scAAV. Clic5 ( n = 10). ( J ) ABR thresholds at 8 weeks of Clic5 +/− ( n = 11), Clic5 −/− ( n = 11), and Clic5 −/− injected with scAVV. Clic5 (n = 7). ( K ) ABR thresholds at 12 weeks of Clic5 +/− ( n = 10), Clic5 −/− ( n = 10), and Clic5 −/− injected with scAAV. Clic5 ( n = 7). ( L ) DPOAE thresholds at 4 weeks of Clic5 +/− ( n = 12), Clic5 −/ − ( n = 12), and Clic5 −/− injected with scAAV. Clic5 ( n = 10). ( M ) ABR thresholds at 3-time points of Clic5 −/− injected with scAAV. Clic5: 4 weeks ( n = 10), 8 weeks ( n = 7), and 12 weeks ( n = 7). ( N ) Freezing behavior duration in a fear conditioning assay at 12 weeks of Clic5 +/− ( n = 14), Clic5 − /− ( n = 14), and Clic5 −/− injected with scAAV. Clic5 ( n = 6). ( O ) Expression rates of scAAV. Clic5 across the cochlea as a function of viral titer, n = 3 for each group. ( P ) ABR thresholds at 4 weeks of Clic5 −/ − injected with scAAV .Clic5 at different viral doses: 1.52 × 10¹³ gc/ml ( n = 10), 1.52 × 10 12 gc/ml ( n = 3) and 1.52 × 10 11 gc/ml ( n = 3). Data information: Statistical tests were two-way ANOVA with Holm–Sidak correction for multiple comparisons for ( D – F , I – N , P ), and one-way ANOVA followed by Tukey correction for multiple comparisons for ( O ). Plots show mean ± SD. ns not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 (Exact P values are provided in Appendix Table S ). Scale bars = 10 μm for ( A , C , H ), 2 μm for ( B ), 0.5 μm for OHC ( G ), and 2 μm for IHC ( G ). .
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    Image Search Results


    ( A ) 3D surface projection of IHCs and OHCs from the cochlear 32 kHz region of Clic5 +/− , Clic5 −/− and Clic5 −/− injected with scAAV. Clic5 at 12 weeks, stained with a CLIC5 (red) and actin (gray). ( B ) Representative confocal images of IHCs from the apical turn of Clic5 +/− and Clic5 −/− injected with scAAV. Clic5 at 12 weeks, stained with a CLIC5 (red), actin (gray), and FLAG tag (green). ( C ) Representative confocal images of IHCs and OHCs from the cochlear 8 kHz region of Clic5 +/− , Clic5 −/− , and Clic5 −/− injected with scAAV. Clic5 at 12 weeks, stained with actin. ( D ) Stereocilia length measurements of IHC at different regions of the cochlea, n = 3 for Clic5 +/− , Clic5 −/− , and Clic5 −/− injected with scAAV. Clic5 . ( E ) Quantification of OHC survival at different regions of the cochlea, n = 3 for Clic5 +/− , Clic5 −/− , and Clic5 −/− injected with scAAV. Clic5 . ( F ) Quantification of IHC survival at different regions of the cochlea, n = 3 for Clic5 +/− , Clic5 −/− , and Clic5 −/− injected with scAAV. Clic5 . ( G ) High-magnification scanning electron microscopy images of outer and inner hair bundles from the cochlea middle-turn of the Clic5 −/− and Clic5 −/− injected with scAAV. Clic5 at 12 weeks. ( H ) Representative images of IHCs and OHCs at 12 weeks at different regions of the cochlea of Clic5 −/ − injected with scAAV. Clic5 . ( I ) ABR thresholds at 4 weeks of Clic5 +/− ( n = 11), Clic5 −/− ( n = 10), and Clic5 −/− injected with scAAV. Clic5 ( n = 10). ( J ) ABR thresholds at 8 weeks of Clic5 +/− ( n = 11), Clic5 −/− ( n = 11), and Clic5 −/− injected with scAVV. Clic5 (n = 7). ( K ) ABR thresholds at 12 weeks of Clic5 +/− ( n = 10), Clic5 −/− ( n = 10), and Clic5 −/− injected with scAAV. Clic5 ( n = 7). ( L ) DPOAE thresholds at 4 weeks of Clic5 +/− ( n = 12), Clic5 −/ − ( n = 12), and Clic5 −/− injected with scAAV. Clic5 ( n = 10). ( M ) ABR thresholds at 3-time points of Clic5 −/− injected with scAAV. Clic5: 4 weeks ( n = 10), 8 weeks ( n = 7), and 12 weeks ( n = 7). ( N ) Freezing behavior duration in a fear conditioning assay at 12 weeks of Clic5 +/− ( n = 14), Clic5 − /− ( n = 14), and Clic5 −/− injected with scAAV. Clic5 ( n = 6). ( O ) Expression rates of scAAV. Clic5 across the cochlea as a function of viral titer, n = 3 for each group. ( P ) ABR thresholds at 4 weeks of Clic5 −/ − injected with scAAV .Clic5 at different viral doses: 1.52 × 10¹³ gc/ml ( n = 10), 1.52 × 10 12 gc/ml ( n = 3) and 1.52 × 10 11 gc/ml ( n = 3). Data information: Statistical tests were two-way ANOVA with Holm–Sidak correction for multiple comparisons for ( D – F , I – N , P ), and one-way ANOVA followed by Tukey correction for multiple comparisons for ( O ). Plots show mean ± SD. ns not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 (Exact P values are provided in Appendix Table S ). Scale bars = 10 μm for ( A , C , H ), 2 μm for ( B ), 0.5 μm for OHC ( G ), and 2 μm for IHC ( G ). .

    Journal: EMBO Molecular Medicine

    Article Title: AAV gene therapy rescues hearing and balance in a model of CLIC5 deafness

    doi: 10.1038/s44321-025-00275-7

    Figure Lengend Snippet: ( A ) 3D surface projection of IHCs and OHCs from the cochlear 32 kHz region of Clic5 +/− , Clic5 −/− and Clic5 −/− injected with scAAV. Clic5 at 12 weeks, stained with a CLIC5 (red) and actin (gray). ( B ) Representative confocal images of IHCs from the apical turn of Clic5 +/− and Clic5 −/− injected with scAAV. Clic5 at 12 weeks, stained with a CLIC5 (red), actin (gray), and FLAG tag (green). ( C ) Representative confocal images of IHCs and OHCs from the cochlear 8 kHz region of Clic5 +/− , Clic5 −/− , and Clic5 −/− injected with scAAV. Clic5 at 12 weeks, stained with actin. ( D ) Stereocilia length measurements of IHC at different regions of the cochlea, n = 3 for Clic5 +/− , Clic5 −/− , and Clic5 −/− injected with scAAV. Clic5 . ( E ) Quantification of OHC survival at different regions of the cochlea, n = 3 for Clic5 +/− , Clic5 −/− , and Clic5 −/− injected with scAAV. Clic5 . ( F ) Quantification of IHC survival at different regions of the cochlea, n = 3 for Clic5 +/− , Clic5 −/− , and Clic5 −/− injected with scAAV. Clic5 . ( G ) High-magnification scanning electron microscopy images of outer and inner hair bundles from the cochlea middle-turn of the Clic5 −/− and Clic5 −/− injected with scAAV. Clic5 at 12 weeks. ( H ) Representative images of IHCs and OHCs at 12 weeks at different regions of the cochlea of Clic5 −/ − injected with scAAV. Clic5 . ( I ) ABR thresholds at 4 weeks of Clic5 +/− ( n = 11), Clic5 −/− ( n = 10), and Clic5 −/− injected with scAAV. Clic5 ( n = 10). ( J ) ABR thresholds at 8 weeks of Clic5 +/− ( n = 11), Clic5 −/− ( n = 11), and Clic5 −/− injected with scAVV. Clic5 (n = 7). ( K ) ABR thresholds at 12 weeks of Clic5 +/− ( n = 10), Clic5 −/− ( n = 10), and Clic5 −/− injected with scAAV. Clic5 ( n = 7). ( L ) DPOAE thresholds at 4 weeks of Clic5 +/− ( n = 12), Clic5 −/ − ( n = 12), and Clic5 −/− injected with scAAV. Clic5 ( n = 10). ( M ) ABR thresholds at 3-time points of Clic5 −/− injected with scAAV. Clic5: 4 weeks ( n = 10), 8 weeks ( n = 7), and 12 weeks ( n = 7). ( N ) Freezing behavior duration in a fear conditioning assay at 12 weeks of Clic5 +/− ( n = 14), Clic5 − /− ( n = 14), and Clic5 −/− injected with scAAV. Clic5 ( n = 6). ( O ) Expression rates of scAAV. Clic5 across the cochlea as a function of viral titer, n = 3 for each group. ( P ) ABR thresholds at 4 weeks of Clic5 −/ − injected with scAAV .Clic5 at different viral doses: 1.52 × 10¹³ gc/ml ( n = 10), 1.52 × 10 12 gc/ml ( n = 3) and 1.52 × 10 11 gc/ml ( n = 3). Data information: Statistical tests were two-way ANOVA with Holm–Sidak correction for multiple comparisons for ( D – F , I – N , P ), and one-way ANOVA followed by Tukey correction for multiple comparisons for ( O ). Plots show mean ± SD. ns not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 (Exact P values are provided in Appendix Table S ). Scale bars = 10 μm for ( A , C , H ), 2 μm for ( B ), 0.5 μm for OHC ( G ), and 2 μm for IHC ( G ). .

    Article Snippet: Imaris 3D Interactive Microscopy Visualization software , Oxford Instruments . , version 10.1.

    Techniques: Injection, Staining, FLAG-tag, Electron Microscopy, Expressing