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rna solution  (Zymo Research)


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    Zymo Research rna solution
    Rna Solution, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 487 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3+12+buffer+solutions/RNA+Extraction+Buffer/pmc10235121-159-1-9
    Average 99 stars, based on 487 article reviews
    rna solution - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Extraction:

    Article Title: Impact of an acute heat shock during in vitro maturation on interleukin 6 and its associated receptor component transcripts in bovine cumulus-oocyte complexes
    Article Snippet: .. After supernatant removal, COCs were lysed in extraction buffer (Quick-RNA Kit; Zymo Research, Irvine, CA, USA) and stored at -80°C until RNA isolation. ..

    Isolation:

    Article Title: Impact of an acute heat shock during in vitro maturation on interleukin 6 and its associated receptor component transcripts in bovine cumulus-oocyte complexes
    Article Snippet: .. After supernatant removal, COCs were lysed in extraction buffer (Quick-RNA Kit; Zymo Research, Irvine, CA, USA) and stored at -80°C until RNA isolation. ..

    Article Title: Low Molecular Weight and High Deacetylation Degree Chitosan Batch Alleviates Pathogenesis, Toxin Accumulation, and Fusarium Gene Regulation in Barley Leaf Pathosystem.
    Article Snippet: .. RNA Extraction and mRNA Sequencing Total RNA was extracted from 100 mg of barley leaves infected with Fg using the RNA isolation kit (Zymo-R2072, Irvine, CA, USA), RNA extraction buffer (50 mM Tris-HCl pH = 8.0, 150 mM LiCl, 5 mM EDTA pH = 8.0, 1% SDS), Trizol reagent, and phenol: chloroform (1:1), 80% ethanol) according to the manufacturer’s protocol. .. The isolated RNA was quantified using a NanoDrop spectrophotometer (NanoDrop Technologies®, Wilmington, DE, USA).

    Article Title: Pi-starvation induced transcriptional changes in barley revealed by a comprehensive RNA-Seq and degradome analyses
    Article Snippet: The samples were measured in two technical and three biological replicates using an Infinite F200 Pro (TECAN, Switzerland). .. Four procedures of RNA isolation were used, depending on the following experiments: (i) small RNA expression level analysis (ddPCR using TaqManTM MicroRNA assays, NGS of small RNAs, Northern hybridization); (ii) shoot transcriptome analysis; (iii) degradome - PARE (Parallel Analysis of RNA Ends) [ ] analysis for mRNA cleaved by miRNA; or (iv) validation of RNA-Seq data using ddPCR. (i) Small RNA expression level analysis RNA isolation was performed using a modified method allowing enrichment of small RNAs, according to the detailed protocol we published before [ ]. (ii) RNA for RNA-Seq RNA was extracted from a 100 mg of shoot sample using RNA extraction buffer [ ] and a Direct-Zol RNA MiniPrep Kit (Zymo Research). .. According to the Lexogen’s SENSE mRNA-Seq Library prep kit v2 user guide DNase treatment step was omitted to avoid RNA hydrolysis. (iii) RNA for degradome analysis Procedure of RNA isolation from barley root and shoot (growing in low-Pi conditions) used for degradome profiling was performed using a method described by German et al. using RNA extraction buffer [ ], along with some modifications that we have described previously [ , ]. (iv) mRNA-Seq data validation To validate the transcript level of significantly changed genes, we used precise dd-PCR analysis.

    Article Title: Low Molecular Weight and High Deacetylation Degree Chitosan Batch Alleviates Pathogenesis, Toxin Accumulation, and Fusarium Gene Regulation in Barley Leaf Pathosystem
    Article Snippet: .. Total RNA was extracted from 100 mg of barley leaves infected with Fg using the RNA isolation kit (Zymo-R2072, Irvine, CA, USA), RNA extraction buffer (50 mM Tris-HCl pH = 8.0, 150 mM LiCl, 5 mM EDTA pH = 8.0, 1% SDS), Trizol reagent, and phenol: chloroform (1:1), 80% ethanol) according to the manufacturer’s protocol. .. The isolated RNA was quantified using a NanoDrop spectrophotometer (NanoDrop Technologies ® , Wilmington, DE, USA).

    RNA Extraction:


    Article Title: Low Molecular Weight and High Deacetylation Degree Chitosan Batch Alleviates Pathogenesis, Toxin Accumulation, and Fusarium Gene Regulation in Barley Leaf Pathosystem.
    Article Snippet: .. RNA Extraction and mRNA Sequencing Total RNA was extracted from 100 mg of barley leaves infected with Fg using the RNA isolation kit (Zymo-R2072, Irvine, CA, USA), RNA extraction buffer (50 mM Tris-HCl pH = 8.0, 150 mM LiCl, 5 mM EDTA pH = 8.0, 1% SDS), Trizol reagent, and phenol: chloroform (1:1), 80% ethanol) according to the manufacturer’s protocol. .. The isolated RNA was quantified using a NanoDrop spectrophotometer (NanoDrop Technologies®, Wilmington, DE, USA).

    Article Title: Pi-starvation induced transcriptional changes in barley revealed by a comprehensive RNA-Seq and degradome analyses
    Article Snippet: The samples were measured in two technical and three biological replicates using an Infinite F200 Pro (TECAN, Switzerland). .. Four procedures of RNA isolation were used, depending on the following experiments: (i) small RNA expression level analysis (ddPCR using TaqManTM MicroRNA assays, NGS of small RNAs, Northern hybridization); (ii) shoot transcriptome analysis; (iii) degradome - PARE (Parallel Analysis of RNA Ends) [ ] analysis for mRNA cleaved by miRNA; or (iv) validation of RNA-Seq data using ddPCR. (i) Small RNA expression level analysis RNA isolation was performed using a modified method allowing enrichment of small RNAs, according to the detailed protocol we published before [ ]. (ii) RNA for RNA-Seq RNA was extracted from a 100 mg of shoot sample using RNA extraction buffer [ ] and a Direct-Zol RNA MiniPrep Kit (Zymo Research). .. According to the Lexogen’s SENSE mRNA-Seq Library prep kit v2 user guide DNase treatment step was omitted to avoid RNA hydrolysis. (iii) RNA for degradome analysis Procedure of RNA isolation from barley root and shoot (growing in low-Pi conditions) used for degradome profiling was performed using a method described by German et al. using RNA extraction buffer [ ], along with some modifications that we have described previously [ , ]. (iv) mRNA-Seq data validation To validate the transcript level of significantly changed genes, we used precise dd-PCR analysis.

    Article Title: Prostaglandin EP2 receptor antagonist ameliorates neuroinflammation in a two-hit mouse model of Alzheimer's disease.
    Article Snippet: .. Sixteen different blood cell parameters were analyzed, including differential white blood cells (WBC), red blood cells (RBC) and platelet counts, distribution width for different cell types, levels of hemoglobin and hematocrit. qRT‐PCR As previously described [52], 10–13 frozen neocortex tissues from each group were homogenized using a sonicator in 1 ml RNA extraction buffer supplied with the Quick-RNA MiniPrep Kit (Zymo Research). ..

    Article Title: Prostaglandin EP2 receptor antagonist ameliorates neuroinflammation in a two-hit mouse model of Alzheimer’s disease
    Article Snippet: .. As previously described [ ], 10–13 frozen neocortex tissues from each group were homogenized using a sonicator in 1 ml RNA extraction buffer supplied with the Quick-RNA MiniPrep Kit (Zymo Research). ..

    Article Title: Low Molecular Weight and High Deacetylation Degree Chitosan Batch Alleviates Pathogenesis, Toxin Accumulation, and Fusarium Gene Regulation in Barley Leaf Pathosystem
    Article Snippet: .. Total RNA was extracted from 100 mg of barley leaves infected with Fg using the RNA isolation kit (Zymo-R2072, Irvine, CA, USA), RNA extraction buffer (50 mM Tris-HCl pH = 8.0, 150 mM LiCl, 5 mM EDTA pH = 8.0, 1% SDS), Trizol reagent, and phenol: chloroform (1:1), 80% ethanol) according to the manufacturer’s protocol. .. The isolated RNA was quantified using a NanoDrop spectrophotometer (NanoDrop Technologies ® , Wilmington, DE, USA).

    Sequencing:

    Article Title: Low Molecular Weight and High Deacetylation Degree Chitosan Batch Alleviates Pathogenesis, Toxin Accumulation, and Fusarium Gene Regulation in Barley Leaf Pathosystem.
    Article Snippet: .. RNA Extraction and mRNA Sequencing Total RNA was extracted from 100 mg of barley leaves infected with Fg using the RNA isolation kit (Zymo-R2072, Irvine, CA, USA), RNA extraction buffer (50 mM Tris-HCl pH = 8.0, 150 mM LiCl, 5 mM EDTA pH = 8.0, 1% SDS), Trizol reagent, and phenol: chloroform (1:1), 80% ethanol) according to the manufacturer’s protocol. .. The isolated RNA was quantified using a NanoDrop spectrophotometer (NanoDrop Technologies®, Wilmington, DE, USA).

    Infection:

    Article Title: Low Molecular Weight and High Deacetylation Degree Chitosan Batch Alleviates Pathogenesis, Toxin Accumulation, and Fusarium Gene Regulation in Barley Leaf Pathosystem.
    Article Snippet: .. RNA Extraction and mRNA Sequencing Total RNA was extracted from 100 mg of barley leaves infected with Fg using the RNA isolation kit (Zymo-R2072, Irvine, CA, USA), RNA extraction buffer (50 mM Tris-HCl pH = 8.0, 150 mM LiCl, 5 mM EDTA pH = 8.0, 1% SDS), Trizol reagent, and phenol: chloroform (1:1), 80% ethanol) according to the manufacturer’s protocol. .. The isolated RNA was quantified using a NanoDrop spectrophotometer (NanoDrop Technologies®, Wilmington, DE, USA).

    Article Title: Low Molecular Weight and High Deacetylation Degree Chitosan Batch Alleviates Pathogenesis, Toxin Accumulation, and Fusarium Gene Regulation in Barley Leaf Pathosystem
    Article Snippet: .. Total RNA was extracted from 100 mg of barley leaves infected with Fg using the RNA isolation kit (Zymo-R2072, Irvine, CA, USA), RNA extraction buffer (50 mM Tris-HCl pH = 8.0, 150 mM LiCl, 5 mM EDTA pH = 8.0, 1% SDS), Trizol reagent, and phenol: chloroform (1:1), 80% ethanol) according to the manufacturer’s protocol. .. The isolated RNA was quantified using a NanoDrop spectrophotometer (NanoDrop Technologies ® , Wilmington, DE, USA).

    RNA Expression:

    Article Title: Pi-starvation induced transcriptional changes in barley revealed by a comprehensive RNA-Seq and degradome analyses
    Article Snippet: The samples were measured in two technical and three biological replicates using an Infinite F200 Pro (TECAN, Switzerland). .. Four procedures of RNA isolation were used, depending on the following experiments: (i) small RNA expression level analysis (ddPCR using TaqManTM MicroRNA assays, NGS of small RNAs, Northern hybridization); (ii) shoot transcriptome analysis; (iii) degradome - PARE (Parallel Analysis of RNA Ends) [ ] analysis for mRNA cleaved by miRNA; or (iv) validation of RNA-Seq data using ddPCR. (i) Small RNA expression level analysis RNA isolation was performed using a modified method allowing enrichment of small RNAs, according to the detailed protocol we published before [ ]. (ii) RNA for RNA-Seq RNA was extracted from a 100 mg of shoot sample using RNA extraction buffer [ ] and a Direct-Zol RNA MiniPrep Kit (Zymo Research). .. According to the Lexogen’s SENSE mRNA-Seq Library prep kit v2 user guide DNase treatment step was omitted to avoid RNA hydrolysis. (iii) RNA for degradome analysis Procedure of RNA isolation from barley root and shoot (growing in low-Pi conditions) used for degradome profiling was performed using a method described by German et al. using RNA extraction buffer [ ], along with some modifications that we have described previously [ , ]. (iv) mRNA-Seq data validation To validate the transcript level of significantly changed genes, we used precise dd-PCR analysis.

    Next-Generation Sequencing:

    Article Title: Pi-starvation induced transcriptional changes in barley revealed by a comprehensive RNA-Seq and degradome analyses
    Article Snippet: The samples were measured in two technical and three biological replicates using an Infinite F200 Pro (TECAN, Switzerland). .. Four procedures of RNA isolation were used, depending on the following experiments: (i) small RNA expression level analysis (ddPCR using TaqManTM MicroRNA assays, NGS of small RNAs, Northern hybridization); (ii) shoot transcriptome analysis; (iii) degradome - PARE (Parallel Analysis of RNA Ends) [ ] analysis for mRNA cleaved by miRNA; or (iv) validation of RNA-Seq data using ddPCR. (i) Small RNA expression level analysis RNA isolation was performed using a modified method allowing enrichment of small RNAs, according to the detailed protocol we published before [ ]. (ii) RNA for RNA-Seq RNA was extracted from a 100 mg of shoot sample using RNA extraction buffer [ ] and a Direct-Zol RNA MiniPrep Kit (Zymo Research). .. According to the Lexogen’s SENSE mRNA-Seq Library prep kit v2 user guide DNase treatment step was omitted to avoid RNA hydrolysis. (iii) RNA for degradome analysis Procedure of RNA isolation from barley root and shoot (growing in low-Pi conditions) used for degradome profiling was performed using a method described by German et al. using RNA extraction buffer [ ], along with some modifications that we have described previously [ , ]. (iv) mRNA-Seq data validation To validate the transcript level of significantly changed genes, we used precise dd-PCR analysis.

    Northern Blot:

    Article Title: Pi-starvation induced transcriptional changes in barley revealed by a comprehensive RNA-Seq and degradome analyses
    Article Snippet: The samples were measured in two technical and three biological replicates using an Infinite F200 Pro (TECAN, Switzerland). .. Four procedures of RNA isolation were used, depending on the following experiments: (i) small RNA expression level analysis (ddPCR using TaqManTM MicroRNA assays, NGS of small RNAs, Northern hybridization); (ii) shoot transcriptome analysis; (iii) degradome - PARE (Parallel Analysis of RNA Ends) [ ] analysis for mRNA cleaved by miRNA; or (iv) validation of RNA-Seq data using ddPCR. (i) Small RNA expression level analysis RNA isolation was performed using a modified method allowing enrichment of small RNAs, according to the detailed protocol we published before [ ]. (ii) RNA for RNA-Seq RNA was extracted from a 100 mg of shoot sample using RNA extraction buffer [ ] and a Direct-Zol RNA MiniPrep Kit (Zymo Research). .. According to the Lexogen’s SENSE mRNA-Seq Library prep kit v2 user guide DNase treatment step was omitted to avoid RNA hydrolysis. (iii) RNA for degradome analysis Procedure of RNA isolation from barley root and shoot (growing in low-Pi conditions) used for degradome profiling was performed using a method described by German et al. using RNA extraction buffer [ ], along with some modifications that we have described previously [ , ]. (iv) mRNA-Seq data validation To validate the transcript level of significantly changed genes, we used precise dd-PCR analysis.

    Hybridization:

    Article Title: Pi-starvation induced transcriptional changes in barley revealed by a comprehensive RNA-Seq and degradome analyses
    Article Snippet: The samples were measured in two technical and three biological replicates using an Infinite F200 Pro (TECAN, Switzerland). .. Four procedures of RNA isolation were used, depending on the following experiments: (i) small RNA expression level analysis (ddPCR using TaqManTM MicroRNA assays, NGS of small RNAs, Northern hybridization); (ii) shoot transcriptome analysis; (iii) degradome - PARE (Parallel Analysis of RNA Ends) [ ] analysis for mRNA cleaved by miRNA; or (iv) validation of RNA-Seq data using ddPCR. (i) Small RNA expression level analysis RNA isolation was performed using a modified method allowing enrichment of small RNAs, according to the detailed protocol we published before [ ]. (ii) RNA for RNA-Seq RNA was extracted from a 100 mg of shoot sample using RNA extraction buffer [ ] and a Direct-Zol RNA MiniPrep Kit (Zymo Research). .. According to the Lexogen’s SENSE mRNA-Seq Library prep kit v2 user guide DNase treatment step was omitted to avoid RNA hydrolysis. (iii) RNA for degradome analysis Procedure of RNA isolation from barley root and shoot (growing in low-Pi conditions) used for degradome profiling was performed using a method described by German et al. using RNA extraction buffer [ ], along with some modifications that we have described previously [ , ]. (iv) mRNA-Seq data validation To validate the transcript level of significantly changed genes, we used precise dd-PCR analysis.

    Biomarker Discovery:

    Article Title: Pi-starvation induced transcriptional changes in barley revealed by a comprehensive RNA-Seq and degradome analyses
    Article Snippet: The samples were measured in two technical and three biological replicates using an Infinite F200 Pro (TECAN, Switzerland). .. Four procedures of RNA isolation were used, depending on the following experiments: (i) small RNA expression level analysis (ddPCR using TaqManTM MicroRNA assays, NGS of small RNAs, Northern hybridization); (ii) shoot transcriptome analysis; (iii) degradome - PARE (Parallel Analysis of RNA Ends) [ ] analysis for mRNA cleaved by miRNA; or (iv) validation of RNA-Seq data using ddPCR. (i) Small RNA expression level analysis RNA isolation was performed using a modified method allowing enrichment of small RNAs, according to the detailed protocol we published before [ ]. (ii) RNA for RNA-Seq RNA was extracted from a 100 mg of shoot sample using RNA extraction buffer [ ] and a Direct-Zol RNA MiniPrep Kit (Zymo Research). .. According to the Lexogen’s SENSE mRNA-Seq Library prep kit v2 user guide DNase treatment step was omitted to avoid RNA hydrolysis. (iii) RNA for degradome analysis Procedure of RNA isolation from barley root and shoot (growing in low-Pi conditions) used for degradome profiling was performed using a method described by German et al. using RNA extraction buffer [ ], along with some modifications that we have described previously [ , ]. (iv) mRNA-Seq data validation To validate the transcript level of significantly changed genes, we used precise dd-PCR analysis.

    RNA sequencing:

    Article Title: Pi-starvation induced transcriptional changes in barley revealed by a comprehensive RNA-Seq and degradome analyses
    Article Snippet: The samples were measured in two technical and three biological replicates using an Infinite F200 Pro (TECAN, Switzerland). .. Four procedures of RNA isolation were used, depending on the following experiments: (i) small RNA expression level analysis (ddPCR using TaqManTM MicroRNA assays, NGS of small RNAs, Northern hybridization); (ii) shoot transcriptome analysis; (iii) degradome - PARE (Parallel Analysis of RNA Ends) [ ] analysis for mRNA cleaved by miRNA; or (iv) validation of RNA-Seq data using ddPCR. (i) Small RNA expression level analysis RNA isolation was performed using a modified method allowing enrichment of small RNAs, according to the detailed protocol we published before [ ]. (ii) RNA for RNA-Seq RNA was extracted from a 100 mg of shoot sample using RNA extraction buffer [ ] and a Direct-Zol RNA MiniPrep Kit (Zymo Research). .. According to the Lexogen’s SENSE mRNA-Seq Library prep kit v2 user guide DNase treatment step was omitted to avoid RNA hydrolysis. (iii) RNA for degradome analysis Procedure of RNA isolation from barley root and shoot (growing in low-Pi conditions) used for degradome profiling was performed using a method described by German et al. using RNA extraction buffer [ ], along with some modifications that we have described previously [ , ]. (iv) mRNA-Seq data validation To validate the transcript level of significantly changed genes, we used precise dd-PCR analysis.

    Modification:

    Article Title: Pi-starvation induced transcriptional changes in barley revealed by a comprehensive RNA-Seq and degradome analyses
    Article Snippet: The samples were measured in two technical and three biological replicates using an Infinite F200 Pro (TECAN, Switzerland). .. Four procedures of RNA isolation were used, depending on the following experiments: (i) small RNA expression level analysis (ddPCR using TaqManTM MicroRNA assays, NGS of small RNAs, Northern hybridization); (ii) shoot transcriptome analysis; (iii) degradome - PARE (Parallel Analysis of RNA Ends) [ ] analysis for mRNA cleaved by miRNA; or (iv) validation of RNA-Seq data using ddPCR. (i) Small RNA expression level analysis RNA isolation was performed using a modified method allowing enrichment of small RNAs, according to the detailed protocol we published before [ ]. (ii) RNA for RNA-Seq RNA was extracted from a 100 mg of shoot sample using RNA extraction buffer [ ] and a Direct-Zol RNA MiniPrep Kit (Zymo Research). .. According to the Lexogen’s SENSE mRNA-Seq Library prep kit v2 user guide DNase treatment step was omitted to avoid RNA hydrolysis. (iii) RNA for degradome analysis Procedure of RNA isolation from barley root and shoot (growing in low-Pi conditions) used for degradome profiling was performed using a method described by German et al. using RNA extraction buffer [ ], along with some modifications that we have described previously [ , ]. (iv) mRNA-Seq data validation To validate the transcript level of significantly changed genes, we used precise dd-PCR analysis.



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