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PHORETIX INTERNATIONAL LIMITED 2-d expression software
2 D Expression Software, supplied by PHORETIX INTERNATIONAL LIMITED, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2-d+expression+software/2d+expression+software/pm23201274-112-10-9
Average 90 stars, based on 1 article reviews
2-d expression software - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Expressing:

Article Title: Bacillus anthracis secretome time course under host-simulated conditions and identification of immunogenic proteins
Article Snippet: Corresponding sera from uninfected humans were used as controls (Cambrex, Charles City, IA). .. Three replicate blots were used for computer analysis using the Phoretix 2D Expression software to identify the immunogenic proteins. .. Protein spots were excised, washed, and trypsin digested from 2-DE gels according to manufacturer's instructions using the Xcise robotic workstation (Shimadzu Biotech, Columbia, MD).

Article Title: Study of urinary proteomes in Anderson-Fabry disease
Article Snippet: Background: Anderson-Fabry disease (AFD) is an X-linked genetic disorder with deficient a-galactosidase A activity.. The main aim of this work was to investigate possible differences in urine proteins between healthy controls and AFD patients and to identify abnormal proteins as potential biomarkers of disease.. Material and methods: We studied 2D electrophoresis images of urine samples collected from AFD patients and healthy subjects.

Article Title: Exposure of Rat Neural Stem Cells to Ethanol Affects Cell Numbers and Alters Expression of 28 Proteins.
Article Snippet: Developing brain cells express many proteins but little is known of how their protein composition responds to chronic exposure to alcohol and/or how such changes might relate to alcohol toxicity.. We used cultures derived from embryonic rat brain (previously shown to contain mostly neural stem cells; rat NSC, rNSC), exposed them to ethanol (25–100 mM) for up to 96 h and studied how they reacted.. Ethanol (50 and 100 mM) reduced cell numbers indicating either compromised cell proliferation, cytotoxicity or both.

Article Title: Altered proteins of the anterior cingulate cortex white matter proteome in schizophrenia.
Article Snippet: Alterations in the circuitry between and within different brain regions including the anterior cingulate cortex (ACC) is implicated in the neuropathology of schizophrenia.. The involvement of white matter in schizophrenia is becoming increasingly apparent with reports of structural, morphological and genetic alterations occurring in the disease.. The 2-DE was employed to reveal significantly altered proteins within the ACC white matter proteome in a schizophrenia cohort (n = 10) relative to controls (n = 10).

Article Title: Antipsychotic induced alteration of growth and proteome of rat neural stem cells.
Article Snippet: Neural stem cells (NSCs) play a crucial role in the development and maturation of the central nervous system and therefore have the potential to target by therapeutic agents for a wide variety of diseases including neurodegenerative and neuropsychiatric illnesses.. It has been suggested that antipsychotic drugs have significant effects on NSC activities.. However, the molecular mechanisms underlying antipsychotic-induced changes of NSC activities, particularly growth and protein expression, are largely unknown.

Software:

Article Title: Bacillus anthracis secretome time course under host-simulated conditions and identification of immunogenic proteins
Article Snippet: Corresponding sera from uninfected humans were used as controls (Cambrex, Charles City, IA). .. Three replicate blots were used for computer analysis using the Phoretix 2D Expression software to identify the immunogenic proteins. .. Protein spots were excised, washed, and trypsin digested from 2-DE gels according to manufacturer's instructions using the Xcise robotic workstation (Shimadzu Biotech, Columbia, MD).

Article Title: Study of urinary proteomes in Anderson-Fabry disease
Article Snippet: Background: Anderson-Fabry disease (AFD) is an X-linked genetic disorder with deficient a-galactosidase A activity.. The main aim of this work was to investigate possible differences in urine proteins between healthy controls and AFD patients and to identify abnormal proteins as potential biomarkers of disease.. Material and methods: We studied 2D electrophoresis images of urine samples collected from AFD patients and healthy subjects.

Article Title: Exposure of Rat Neural Stem Cells to Ethanol Affects Cell Numbers and Alters Expression of 28 Proteins.
Article Snippet: Developing brain cells express many proteins but little is known of how their protein composition responds to chronic exposure to alcohol and/or how such changes might relate to alcohol toxicity.. We used cultures derived from embryonic rat brain (previously shown to contain mostly neural stem cells; rat NSC, rNSC), exposed them to ethanol (25–100 mM) for up to 96 h and studied how they reacted.. Ethanol (50 and 100 mM) reduced cell numbers indicating either compromised cell proliferation, cytotoxicity or both.

Article Title: Altered proteins of the anterior cingulate cortex white matter proteome in schizophrenia.
Article Snippet: Alterations in the circuitry between and within different brain regions including the anterior cingulate cortex (ACC) is implicated in the neuropathology of schizophrenia.. The involvement of white matter in schizophrenia is becoming increasingly apparent with reports of structural, morphological and genetic alterations occurring in the disease.. The 2-DE was employed to reveal significantly altered proteins within the ACC white matter proteome in a schizophrenia cohort (n = 10) relative to controls (n = 10).

Article Title: Antipsychotic induced alteration of growth and proteome of rat neural stem cells.
Article Snippet: Neural stem cells (NSCs) play a crucial role in the development and maturation of the central nervous system and therefore have the potential to target by therapeutic agents for a wide variety of diseases including neurodegenerative and neuropsychiatric illnesses.. It has been suggested that antipsychotic drugs have significant effects on NSC activities.. However, the molecular mechanisms underlying antipsychotic-induced changes of NSC activities, particularly growth and protein expression, are largely unknown.

other:

Article Title: Early presence of an enolase in the oviposition injecta of the aphid parasitoid Aphidius ervi analyzed with chitosan beads as artificial hosts.
Article Snippet: Maternal factors of female wasps that are injected into hosts with their eggs at oviposition play a major role in strategies used by insect parasitoids to overcome host immunity, and to regulate host physiology during early stages of parasitism.. Here, we attempted to precisely determine and compare the protein patterns injected by the endoparasitoid Aphidius ervi into two different host systems.. Chitosan beads of aphid size designed as artificial and physiologically inert hosts were used as oviposition medium, to be compared with the natural aphid host as young nymphs of Macrosiphum euphorbiae.

Article Title: A protease activity-depleted environment for heterologous proteins migrating towards the leaf cell apoplast.
Article Snippet: The 2-D gels were stained with Coomassie Blue, digitalized with an Amersham Image Scanner (GE Healthcare, Baie d’Urfé, QC, Canada) and analysed using the Phoretix 2-D Expression software, v. 2005 (NonLinear USA, Durham NC) (Badri et al., 2009b), with three biological (plant) replicates per treatment to allow for statistical analyses.



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Silver-stained <t>two-dimensional</t> gel of proteins extracted from Medicago sativa leaves grown under control (A) and drought (B) conditions. In the first dimension, 125 mg of total protein was loaded on a 18 cm IEF strip with a linear gradient of pH 4–7. The second dimension was conducted in 12% polyacrylamide (w/v) gels (20×20 cm) (for details, see Materials and methods). The gel image analyses conducted with Progenesis SameSpots software v3.0 and the subsequent mass spectrometry analyses identified up to 26 proteins (marked by arrows) that, statistically, were involved in the plant response to drought.
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Silver-stained <t>two-dimensional</t> gel of proteins extracted from Medicago sativa leaves grown under control (A) and drought (B) conditions. In the first dimension, 125 mg of total protein was loaded on a 18 cm IEF strip with a linear gradient of pH 4–7. The second dimension was conducted in 12% polyacrylamide (w/v) gels (20×20 cm) (for details, see Materials and methods). The gel image analyses conducted with Progenesis SameSpots software v3.0 and the subsequent mass spectrometry analyses identified up to 26 proteins (marked by arrows) that, statistically, were involved in the plant response to drought.
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Image Search Results


Silver-stained two-dimensional gel of proteins extracted from Medicago sativa leaves grown under control (A) and drought (B) conditions. In the first dimension, 125 mg of total protein was loaded on a 18 cm IEF strip with a linear gradient of pH 4–7. The second dimension was conducted in 12% polyacrylamide (w/v) gels (20×20 cm) (for details, see Materials and methods). The gel image analyses conducted with Progenesis SameSpots software v3.0 and the subsequent mass spectrometry analyses identified up to 26 proteins (marked by arrows) that, statistically, were involved in the plant response to drought.

Journal: Journal of Experimental Botany

Article Title: Plant physiology and proteomics reveals the leaf response to drought in alfalfa ( Medicago sativa L.)

doi: 10.1093/jxb/erq249

Figure Lengend Snippet: Silver-stained two-dimensional gel of proteins extracted from Medicago sativa leaves grown under control (A) and drought (B) conditions. In the first dimension, 125 mg of total protein was loaded on a 18 cm IEF strip with a linear gradient of pH 4–7. The second dimension was conducted in 12% polyacrylamide (w/v) gels (20×20 cm) (for details, see Materials and methods). The gel image analyses conducted with Progenesis SameSpots software v3.0 and the subsequent mass spectrometry analyses identified up to 26 proteins (marked by arrows) that, statistically, were involved in the plant response to drought.

Article Snippet: Images of the two-dimensional gels were acquired with the ProXPRESS 2D proteomic Imaging System and analysed using Phoretix 2-D Expression Software v2004 (Nonlinear Dynamics, Newcastle upon Tyne, UK).

Techniques: Staining, Two-Dimensional Gel Electrophoresis, Stripping Membranes, Software, Mass Spectrometry

Annotation of up/down-regulated identified spots following drought in silver stained  two-dimensional  electrophoresis gels of leaves Spot no. represents the number of proteins assigned. Spot volume (%) is an estimation of relative protein abundance. The pI and molecular mass ( M r ) values shown are the theoretical and experimental values. SC represents the protein sequence coverage (%) score, which is the Mascot score of the in-solution digestion protocol. Function, the predicted protein function is assigned according to the NCBInr-protein sequence database.

Journal: Journal of Experimental Botany

Article Title: Plant physiology and proteomics reveals the leaf response to drought in alfalfa ( Medicago sativa L.)

doi: 10.1093/jxb/erq249

Figure Lengend Snippet: Annotation of up/down-regulated identified spots following drought in silver stained two-dimensional electrophoresis gels of leaves Spot no. represents the number of proteins assigned. Spot volume (%) is an estimation of relative protein abundance. The pI and molecular mass ( M r ) values shown are the theoretical and experimental values. SC represents the protein sequence coverage (%) score, which is the Mascot score of the in-solution digestion protocol. Function, the predicted protein function is assigned according to the NCBInr-protein sequence database.

Article Snippet: Images of the two-dimensional gels were acquired with the ProXPRESS 2D proteomic Imaging System and analysed using Phoretix 2-D Expression Software v2004 (Nonlinear Dynamics, Newcastle upon Tyne, UK).

Techniques: Staining, Electrophoresis, Sequencing, Binding Assay