Journal: iScience

Article Title: A 2B adenosine receptor activation and modulation by protein kinase C

doi: 10.1016/j.isci.2023.107178

Figure Lengend Snippet: Probing the mechanisms of enhancement of cAMP accumulation by PMA treatment (A and B) Effect of PMA pretreatment on forskolin (A) and CTX (B)-induced cAMP accumulation in H9C2 cells. Cells were pretreated with PMA (1 μM) for 40 min followed by addition of forskolin (10 μM) and incubate for 20 min or CTX (0.5 μg/mL) for 60 min. PTX (200 ng/mL) was incubated with cells overnight. The bar graph is shown in mean ± S.E.M. of at least three individual experiments performed in triplicate. #p < 0.05, significantly different from CTX group (Student’s t test). CTX, cholera toxin. PTX, pertussis toxin. The mean value of maximal cAMP accumulation induced by 10 μM of forskolin was set as 100%. (C–F) Effects of kinase inhibitors and A 2B AR antagonists on enhancement of cAMP accumulation induced by PMA pretreatment. The maximum activation level of by NECA (10 μM) was set as 100%. The background of each individual cell type was set as 0%. Cells were incubated with PTX (200 ng/mL) overnight and with antagonists or inhibitors for 20 min before addition of PMA (1 μM) and incubated for 40 min, followed by addition of the A 2B agonist BAY and incubation continued for additional 20 min. PTX, pertussis toxin. The concentration of PSB603, MRS1754, GF109203X or GO6983 used was 1 μM; 10 μM H89 was used. (G) Time-course of PMA (1 μM)-induced increase of ERK1/2 activity. GO6983, H89, or PSB603 (1 μM) was added 20 min before PMA addition. Graphs shown are from three individual experiments performed in triplicate. ∗p < 0.05 (significantly different for the corresponding values in “+PMA” group, One-way ANOVA, Tukey’s post hoc test).

Article Snippet: PSB603 , Tocris , Cat. No. 3198/10.

Techniques: Incubation, Activation Assay, Concentration Assay, Activity Assay

Journal: iScience

Article Title: A 2B adenosine receptor activation and modulation by protein kinase C

doi: 10.1016/j.isci.2023.107178

Figure Lengend Snippet: Intracellular calcium mobilization in H9C2 cells and HEK293-A 2B cells (A and B) H9C2. (C,D). HEK293-A 2B . (B,C) Cells were incubated with 1 μM PSB603, PMA, or YM254890 for 20 min. For the PMA+GO6983 group, GO6983 (1 μM) was added 20 min followed by addition of PMA (1 μM) for 20 min, and intracellular calcium mobilization was measured after the addition of agonists using FLIPR Tetra as described in Methods section. D. Comparison of NECA- and PKC-induced calcium mobilization. Error bars represent SEM from at least three independent experiments performed in triplicate.

Article Snippet: PSB603 , Tocris , Cat. No. 3198/10.

Techniques: Incubation, Comparison

Journal: iScience

Article Title: A 2B adenosine receptor activation and modulation by protein kinase C

doi: 10.1016/j.isci.2023.107178

Figure Lengend Snippet:

Article Snippet: PSB603 , Tocris , Cat. No. 3198/10.

Techniques: Membrane, Knock-Out, Amplified Luminescent Proximity Homogenous Assay, Recombinant, Software

Journal: Journal of Clinical Investigation

Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice

doi: 10.1172/jci34203

Figure Lengend Snippet: Figure 1 VILI in mice gene-targeted for individual ARs. Previously characterized A1AR–/– (A; ref. 29), A2AAR–/– (B; ref. 30), A2BAR–/– (C), or A3AR–/– mice (D; ref. 22) or corresponding littermate controls were exposed to VILI, and survival times were determined during VILI. Mechanical ventilation was applied using pressure-con- trolled settings (inspiratory pressure of 35 mbar, inspired oxygen concentration 100%; respiratory rate and inspiratory/expiratory ratio were adjusted to maintain normal pH) until a cardiac standstill was observed in the surface electrocardio- gram. Note the significantly attenuated survival of A2BAR–/– mice (C; P < 0.001, n = 8). Albumin concentration in the BAL fluid was determined by ELISA. For this purpose, the mice were mechanically ventilated using pressure-controlled venti- lation with an inspired oxygen concentration of 100% for 180 minutes at 45 mbar. Note the significantly increased albumin concentration in the BAL fluid of A2BAR–/– mice (C; P < 0.001, n = 6).

Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10–3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10–7 M, 300 μl intratracheally; Tocris); or vehicle (13).

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Journal of Clinical Investigation

Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice

doi: 10.1172/jci34203

Figure Lengend Snippet: Figure 2 VILI in mice gene-targeted for the A2BAR. (A–H) A2BAR–/– mice or littermate controls (A2BAR+/+) were mechanically ventilated using pressure- controlled ventilation with an inspired oxygen concentration of 100% over 180 minutes at 45 mbar. (A) Following ventilation, lungs were excised en bloc and weighed. Lungs were lyophilized for 48 hours, and lung water content (mg lung water/mg dry tissue) was determined. Results are presented as mean ± SD (n = 6). (B) To assess pulmonary gas exchange, blood gas analyses were performed by obtaining arterial blood via cardiac puncture. Analysis was performed immediately, and the ratio of the arterial partial pressure of oxygen (PaO2) to the fraction of inspired oxygen (FiO2) was determined. Results are presented as mean ± SD (n = 6). (C) Pulmonary neutrophil accumulation was quantified using a MPO assay. MPO activity was assessed using a spectrophotometric reaction with O-dianisidine hydrochloride. Absorbance at 450 nm was measured and reported as difference in OD (ΔOD) over 5 minutes. Results are presented as mean ± SD (n = 6). (D–I) TNF-α, IL-6, KC, IL-10, NF-κB, and IκBα levels were evaluated in lung tissue homogenates using a mouse ELISA. Results are presented as mean ± SD (n = 6). (J) For quantification of histological tissue damage by VILI following 180 min ventilation, VILI scores were assessed in A2BAR–/– or corresponding littermate control mice. Results are displayed as median (midline within boxes) and range (bars above and below boxes) (n = 4). (K) One of 4 representative photomicrographs (original magnification, ×200) stained with hematoxylin and eosin is displayed.

Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10–3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10–7 M, 300 μl intratracheally; Tocris); or vehicle (13).

Techniques: Concentration Assay, MPO Assay, Activity Assay, Enzyme-linked Immunosorbent Assay, Control, Staining

Journal: Journal of Clinical Investigation

Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice

doi: 10.1172/jci34203

Figure Lengend Snippet: Figure 3 Transcriptional consequences of mechanical ventilation on AR expression. (A) C57BL/6 mice were mechanically ventilated (inspiratory pressure of 35 mbar, 100% oxygen). After the indicated time periods, lungs were harvested, total RNA was isolated, and A1AR, A2AAR, A2BAR, and A3AR mRNA levels were determined by real-time RT-PCR. Data were calculated relative to the internal housekeeping gene (β-actin) and are expressed as mean fold change compared with control (0 min ventilation) ± SD at each indicated time (n = 4). Note selective induction of the A2BAR gene dur- ing high-pressure ventilation (10-fold, P < 0.01; n = 4). (B) Comparative gene expression of pulmonary ARs using real- time PCR. Relative expression levels in untreated controls or in mice after 180 min mechanical ventilation (inspiratory pressure of 35 mbar, 100% oxygen) are shown. Values are expressed as mean ± SEM (n = 4). *P < 0.05 compared with A2AAR. (C) Mice were mechanically ventilated (35 mbar inspiratory pressure, 100% oxygen), and lungs were har- vested at the indicated time points, shock frozen, and lysed, and proteins were resolved by SDS-PAGE. Resultant west- ern blots were probed with anti-A2BAR antibodies. To con- trol for loading conditions, blots were stripped and reprobed for actin expression. One representative experiment of 3 is shown. (D) To examine the influence of mechanical venti- lation on pulmonary A2BAR expression patterns, C57BL/6 mice were ventilated in a pressure-controlled setting over 0 h or 3 h (35 mbar inspiratory pressure, 100% inspired oxy- gen concentration). Lungs were stained with antibodies for A2BAR. IgG controls were used at identical concentrations and staining conditions as the target primary antibodies (original magnification, ×400; n = 4).

Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10–3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10–7 M, 300 μl intratracheally; Tocris); or vehicle (13).

Techniques: Expressing, Isolation, Quantitative RT-PCR, Control, Gene Expression, Real-time Polymerase Chain Reaction, SDS Page, Concentration Assay, Staining

Journal: Journal of Clinical Investigation

Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice

doi: 10.1172/jci34203

Figure Lengend Snippet: Figure 4 cAMP levels and PKA activity during VILI. (A and B) To assess cAMP levels and PKA activ- ity in pulmonary tissues during VILI, A2BAR–/– mice and littermate controls were mechani- cally ventilated using pressure-controlled ventilation with an inspired oxygen concentration of 100% over 180 minutes at 45 mbar. Animals were euthanized, and lungs were perfused with 5 ml of PBS through the right ventricle. Lungs were excised, shock-frozen utilizing liquid nitrogen, and mechanically homogenized. cAMP levels (A) and PKA activity (B) were determined by ELISA. Note that the increases in cAMP and PKA activity associated with mechanical ventilation were abolished in A2BAR–/– mice. (C) Treatment with the specific β2-adrenergic agonist zinterol during VILI. Control C57BL/6 mice were treated with 300 μl 10–7 M intratracheal zinterol, and baseline cAMP levels were determined in lung homogenates using a competitive immunoassay kit. (D) Control C57BL/6 mice were treated with 300 μl 10–7 M intratracheal zinterol or vehicle control, followed by mechanical ventila- tion using pressure-controlled settings at an inspired oxygen concentration of 100% and 45 mbar inspiratory pressure for 0 or 180 min. Albumin concentration in the BAL fluid was determined by murine ELISA. (E) Survival times during VILI after 300 μl 10–7 M intratracheal zinterol or vehicle treatment. Mechanical ventilation was applied using pressure-controlled settings (inspiratory pressure of 35 mbar, inspired oxygen concentration 100%, respiratory rate and inspiratory/expiratory ratio were adjusted to maintain normal pH) until a cardiac standstill was observed in the surface electrocardiogram.

Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10–3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10–7 M, 300 μl intratracheally; Tocris); or vehicle (13).

Techniques: Activity Assay, Concentration Assay, Enzyme-linked Immunosorbent Assay, Control

Journal: Journal of Clinical Investigation

Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice

doi: 10.1172/jci34203

Figure Lengend Snippet: Figure 5 A2BAR antagonist treatment PSB1115 during VILI. (A–C), A2BAR+/+, A2AAR–/–, and A2BAR–/– mice and their corresponding littermate controls were treated with 10 mg/kg PSB1115 or vehicle 30 minutes prior to induction of anesthesia. Mechanical ventilation was begun, and mice were ventilated using pressure-controlled settings (inspiratory pressure of 45 mbar, 100% inspired oxygen concentration) until a cardiac standstill was observed in the surface electrocardiogram (P < 0.01, n = 8). (D) Mechanical ventilation was begun, and mice were ventilated for 0 or 180 minutes using pressure-controlled settings (inspiratory pressure of 45 mbar, 100% inspired oxygen concentration). Albumin concentration in the BAL fluid was determined by ELISA (n = 6). (E) Pulmonary neutrophil sequestration was quantified using a MPO assay. MPO activity was assessed using a spectrophotometric reaction with O-dianisidine hydrochloride. Absorbance at 450 nm was measured and reported as differ- ence in OD over 5 minutes (n = 6). (F–H) TNF-α, NF-κB, and IL-10 levels were evaluated in lung tissue homogenates using a murine ELISA (n = 6). (I) To assess pulmonary gas exchange, blood gas analyses were performed by obtaining arterial blood via cardiac puncture. Analysis was performed immediately, and the ratio of the arterial partial pressure of oxygen to the fraction of inspired oxygen was determined. Results are presented as mean ± SD (n = 6).

Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10–3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10–7 M, 300 μl intratracheally; Tocris); or vehicle (13).

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, MPO Assay, Activity Assay

Journal: Journal of Clinical Investigation

Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice

doi: 10.1172/jci34203

Figure Lengend Snippet: Figure 6 A2BAR agonist (BAY 60- 6583) treatment. (A–C) A2BAR+/+, A2AAR–/–, and A2BAR–/– mice and their cor- responding littermate controls were treated with 2 mg/kg BAY 60-6583 or vehicle 30 minutes prior to induction of anesthe- sia. Mechanical ventilation was begun, and mice were ventilated using pressure- controlled settings (inspirato- ry pressure of 45 mbar, 100% inspired oxygen concentra- tion) until a cardiac standstill was observed in the surface electrocardiogram (P < 0.01, n = 8). In other studies, albu- min concentrations in the BAL fluid were determined by ELISA after mechanical ventilation using pressure- controlled settings with an inspired oxygen concentration of 100% for 180 minutes at 45 mbar. (D) Pulmonary neutrophil sequestration was quantified using a MPO assay (n = 6). (E–G) TNF-α, NF-κB, and IL-10 levels were evaluated in lung tissue homogenates using murine ELISA (n = 6). (H) To assess pulmonary gas exchange, blood gas analyses were per- formed by obtaining arterial blood via cardiac puncture. The ratio of the arterial partial pressure of oxygen to the frac- tion of inspired oxygen was determined. Results are pre- sented as mean ± SD (n = 6).

Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10–3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10–7 M, 300 μl intratracheally; Tocris); or vehicle (13).

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, MPO Assay

Journal: Journal of Clinical Investigation

Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice

doi: 10.1172/jci34203

Figure Lengend Snippet: Figure 7 A2BAR signaling during LPS-induced lung injury. (A and B) A2BAR–/– and A2BAR+/+ mice were exposed to LPS inhalation for 30 minutes. Twenty-four hours after LPS exposure, TNF-α in lung tis- sue homogenates and albumin concentration in the BAL fluid were determined using a murine ELISA (n = 6). (C and D) A2BAR+/+ mice received 2 mg/kg BAY 60-6583 i.p. or were treated with vehicle 30 minutes prior to LPS inhalation. (C) TNF-α levels in lung tissues and (D) albumin concentrations in the BAL fluid (n = 6).

Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10–3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10–7 M, 300 μl intratracheally; Tocris); or vehicle (13).

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Journal of Clinical Investigation

Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice

doi: 10.1172/jci34203

Figure Lengend Snippet: Figure 8 VILI in A2BAR bone marrow–chimeric mice. A2BAR bone marrow–chimeric mice were subjected to VILI using mechanical ventila- tion for 180 minutes at an inspiratory pres- sure of 45 mbar and 100% inspired oxygen concentration. (A) Albumin concentration in the BAL fluid was determined by ELISA. WT/WT, A2BAR+/+/A2BAR+/+; KO/WT, A2BAR–/–/A2BAR+/+; KO/KO, A2BAR–/–/ A2BAR–/–; WT/KO, A2BAR+/+/A2BAR–/–. (B) Following ventilation at the indicated settings, lungs were excised en bloc and weighed. Lungs were lyophilized for 48 hours, and lung water content (mg lung water/mg dry tissue) was determined. Note the increased albu- min concentration and lung water content in A2BAR–/–/A2BAR–/– and A2BAR+/+/A2BAR–/– mice compared with A2BAR+/+/A2BAR+/+ mice (P < 0.001). (C) Pulmonary neutrophil sequestration was quantified using a MPO assay. MPO activity was assessed using a spectrophotometric reaction with O-dianisi- dine hydrochloride. Absorbance at 450 nm was measured and reported as difference in OD over 5 minutes. (D–F) TNF-α, IL-6, and KC levels were evaluated in lung tissue homogenates using a mouse ELISA. Note the similar degree of pulmonary inflamma- tion in A2BAR–/–/A2BAR+/+ and A2BAR+/+/ A2BAR–/– mice compared with A2BAR+/+/ A2BAR+/+ mice. n = 6.

Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10–3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10–7 M, 300 μl intratracheally; Tocris); or vehicle (13).

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, MPO Assay, Activity Assay

Journal: Journal of Clinical Investigation

Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice

doi: 10.1172/jci34203

Figure Lengend Snippet: Figure 9 BAY 60-6583–dependent lung protection during VILI in A2BAR bone marrow–chimeric mice. (A–D) A2BAR bone marrow–chimeric mice were treated with BAY 60-6583 (2 mg/kg) 30 minutes prior to induction of anesthesia. Mechanical ventilation was begun, and mice were ven- tilated using an inspiratory pressure of 45 mbar, 100% inspired oxygen concentration, for 180 minutes. Results are presented as mean ± SD. n = 6. (A) Albumin concentration in the BAL fluid was determined by ELISA. (B) Following mechanical ventilation, lungs were excised en bloc and weighed. Lungs were then lyophilized for 48 hours, and lung water content (mg lung water/mg dry tissue) was determined. Capillary- alveolar barrier protection was observed only in A2BAR+/+/A2BAR+/+ and A2BAR–/–/A2BAR+/+ mice (P < 0.001; n = 6). (C) Pulmonary neu- trophil sequestration was quantified using a MPO assay. MPO activity was assessed using a spectrophotometric reaction with O-dianisidine hydrochloride. Absorbance at 450 nm was measured and reported as difference in OD over 5 minutes. (D) TNF-α levels were evaluated in lung tissue homogenates using a mouse ELISA. Note the similar lev- els of pulmonary inflammation in A2BAR–/–/A2BAR+/+ and A2BAR+/+/ A2BAR–/– mice.

Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10–3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10–7 M, 300 μl intratracheally; Tocris); or vehicle (13).

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, MPO Assay, Activity Assay

Journal: Journal of Clinical Investigation

Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice

doi: 10.1172/jci34203

Figure Lengend Snippet: Figure 10 Contribution of A2BAR signaling to AFC during VILI. (A) To determine whether A2BAR signaling affects pulmonary fluid transport, we measured AFC using a mechanically ventilated live mouse model. A2BAR–/– mice and littermate controls were mechanically ventilated in a pressure-controlled setting at 45 mbar for 0 to 180 minutes. AFC was measured by instilling 300 μl of iso-osmolar 0.9% NaCl solution with 5% BSA. Mechanical ventilation was continued for 30 minutes, and AFC was measured in the presence or absence of the ENaC inhibitor amiloride (1 mM). *P < 0.01 compared with no amiloride. n = 8. (B) Influence of A2BAR antagonist PSB1115 on AFC during VILI. Follow- ing induction of VILI, control mice received PSB1115 (1 μM) alone or in combination with amiloride (1 mM), and AFC was determined. *P < 0.01 compared with no amiloride. n = 8. (C) VILI was induced in A2BAR bone marrow–chimeric mice, and AFC was determined. *P < 0.001 compared with A2BAR+/+/A2BAR+/+. n = 8. (D) Influence of A2BAR agonist BAY 60-6583 on AFC during VILI. Following induction of VILI, control mice received BAY 60-6583 (1 μM) alone or in combination with amiloride (1 mM), and AFC was determined *P < 0.01 compared with no amiloride. n = 8. (E and F) Control mice were treated with intratracheal BAY 60-6583 (1 μM, 100 μl) and/or amiloride (1 mM) or vehicle following tracheotomy and initiation of mechanical ventilation. Mice were ventilated using pressure-controlled settings (inspiratory pressure of 45 mbar, 100% inspired oxygen concentration) until a cardiac standstill was observed in the surface electrocardiogram. Note that the longer survival time during VILI with A2BAR agonist treatment was abolished following amiloride treatment (n = 8).

Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10–3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10–7 M, 300 μl intratracheally; Tocris); or vehicle (13).

Techniques: Control, Concentration Assay

Journal: Journal of Clinical Investigation

Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice

doi: 10.1172/jci34203

Figure Lengend Snippet: Figure 11 Influence of β2-adrenergic and/or A2BAR signaling on AFC during VILI. (A) Epinephrine plasma levels in A2BAR–/– and A2BAR+/+ mice that were mechanically ventilated in a pressure-controlled setting at 45 mbar over 180 minutes. (B) Basal cAMP levels in lung tissue from A2BAR+/+ mice that were treated with zinterol and/or BAY 60-6583. (C and D) To determine β2-adrenergic and A2BAR signaling effects on pulmonary fluid trans- port, A2BAR–/– and A2BAR+/+ mice were mechanically ventilated in a pressure-controlled setting at 45 mbar for 0 to 180 minutes. AFC was measured by instilling 300 μl of iso-osmolar 0.9% NaCl solution with 5% BSA. Mechanical ventilation was continued for 30 minutes, and AFC was measured in the presence or absence of the nonselective β-adrenergic receptor antagonist pro- pranolol (intratracheal instillation of 10–4 M propranolol combined with 3 mg/kg i.p.) with or without BAY 60-6583 (10–3 M to the instilled fluid) or in the presence or absence of the β-adrenergic agonist zinterol (intratra- cheal, 10–7 M). *P < 0.01 compared with no propranolol (C) or zinterol (D), by ANOVA with Bonferroni post-hoc test. n = 8. In subsets of experiments, either proprano- lol or zinterol were added together with BAY 60-6583 10–3 M to the instilled fluid. §P < 0.01 compared with propranolol alone (C) or zinterol alone (D), by ANOVA with Bonferroni post-hoc test. n = 8.

Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10–3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10–7 M, 300 μl intratracheally; Tocris); or vehicle (13).

Techniques: Clinical Proteomics

Journal: Bioengineered

Article Title: PSB0788 ameliorates maternal inflammation‐induced periventricular leukomalacia-like injury

doi: 10.1080/21655979.2022.2061296

Figure Lengend Snippet: PSB0788 treatment increased MBP expression. (a) Intraperitoneal injection of PSB0788 at E19 was performed to observe changes in oligodendrocytes at all stages in brains of offspring rats at P7. (b) Immunohistochemical results showed that the number of MBP positive cells in the shoulder of the corpus callosum in the PVL-PSB group was significantly increased compared to the PVL-CON group. Scale bar .100 μm. (c) The results of western blot showed that the level of MBP protein in the cerebral white matter of offspring rats in the PVL-PSB group was increased significantly. Data were expressed as mean ±SD (n = 5). # P < 0.05 for student’s t-test.

Article Snippet: SD rats in the selective A 2B AR antagonist group (PVL-PSB group) were intraperitoneally injected with LPS on E17 and E18, and 10 μg/kg PSB0788 (Tocris Bioscience) on E19.

Techniques: Expressing, Injection, Immunohistochemical staining, Western Blot

Journal: Bioengineered

Article Title: PSB0788 ameliorates maternal inflammation‐induced periventricular leukomalacia-like injury

doi: 10.1080/21655979.2022.2061296

Figure Lengend Snippet: PSB0788 treatment of PVL promoted the development and differentiation of OPCs. (a) The number of NG2 positive cells in the shoulder of the corpus callosum in the PVL-PSB group was higher than in the PVL-CON group. Scale bar .20 μm.(b) NG2 protein level increased in the cerebral white matter of offspring rats in the PVL-PSB group. (c) The number of CC-1/Olig2 positive cells in the shoulder of the corpus callosum increased in the PVL-PSB group. Scale bar .100 μm.(d) Oligodendrocytes in the PVL-PSB group tended to be normal. Scale bar .2 μm. Data were expressed as mean ±SD (n = 5). # P < 0.05 for student’s t-test.

Article Snippet: SD rats in the selective A 2B AR antagonist group (PVL-PSB group) were intraperitoneally injected with LPS on E17 and E18, and 10 μg/kg PSB0788 (Tocris Bioscience) on E19.

Techniques:

Journal: Bioengineered

Article Title: PSB0788 ameliorates maternal inflammation‐induced periventricular leukomalacia-like injury

doi: 10.1080/21655979.2022.2061296

Figure Lengend Snippet: PSB0788 treatment activated anti-inflammatory microglia. (a) The level of inflammatory cytokines IL-1 β, IL-6 and TNF-α were not significantly different in the PVL-PSB group from those in the PVL-CON group, while IL-10 was significantly increased in the PVL-PSB group. # P < 0.05 for student’s t-test.

Article Snippet: SD rats in the selective A 2B AR antagonist group (PVL-PSB group) were intraperitoneally injected with LPS on E17 and E18, and 10 μg/kg PSB0788 (Tocris Bioscience) on E19.

Techniques:

Journal: Toxicological Sciences

Article Title: Late Protective Effect of Netrin-1 in the Murine Acetaminophen Hepatotoxicity Model

doi: 10.1093/toxsci/kfaa041

Figure Lengend Snippet: The adenosine A2B receptor antagonist PSB1115 blocked protective effects of netrin-1. Fasted C57BL/6J mice were co-treated with 300 mg/kg acetaminophen (APAP) and netrin-1 (1 µg/mouse) or saline as control, with some mice also receiving PSB1115. Those mice received a second dose of PDB115 6 h after APAP, followed by sacrifice at 24 h and collection of blood and liver tissues. Plasma alanine aminotransferase activity (A) was measured and hematoxylin and eosin (B) and TUNEL (C) staining carried out on liver sections (×50 magnification). Data represent means ± SE of n = 5–10 animals per group. *p < .05 (compared with APAP alone). #p < .05 (compared with APAP+netrin-1).

Article Snippet: In some experiments, mice were cotreated with the A2BAR antagonist PSB1115 (Santa Cruz Biotechnology) (5 mg/kg, i.p.) dissolved in saline, followed by a second treatment with PSB1115 6 h after APAP, and euthanized at 24 h after APAP.

Techniques: Saline, Control, Clinical Proteomics, Activity Assay, TUNEL Assay, Staining

Journal: Toxicological Sciences

Article Title: Late Protective Effect of Netrin-1 in the Murine Acetaminophen Hepatotoxicity Model

doi: 10.1093/toxsci/kfaa041

Figure Lengend Snippet: Blocking adenosine A2B receptor attenuated netrin-1-induced macrophage infiltration. Fasted mice were co-treated with 300 mg/kg acetaminophen (APAP) and netrin-1 (1 µg/mouse) or saline as control, with some mice also receiving PSB1115 at 0 and 6 h after APAP. Mice were sacrificed at 24 h and liver tissue sections used for immunohistochemistry staining of F4/80-positive macrophages (×50 magnification). Panels on the right represent an enlargement of the areas defined on the left.

Article Snippet: In some experiments, mice were cotreated with the A2BAR antagonist PSB1115 (Santa Cruz Biotechnology) (5 mg/kg, i.p.) dissolved in saline, followed by a second treatment with PSB1115 6 h after APAP, and euthanized at 24 h after APAP.

Techniques: Blocking Assay, Saline, Control, Immunohistochemistry, Staining