Review



ythdf3 mutants  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Addgene inc ythdf3 mutants
    (A) Numbers of male and female fish of each genotype. Sibling control and double <t>ythdf2;ythdf3</t> homozygotes were offspring from the same cross, depicted on top. (B) Gonad histology of double homozygous ( ythdf2 −/− ; ythdf3 −/− ) and sibling control fish from the cross in (A). At 27 dpf, mutants exhibit less developed juvenile ovaries than controls. At 35 dpf, 6 sibling fish had adult ovaries and 8 had testes, while all 12 ythdf2 −/− ; ythdf3 −/− fish had testes. I, stage I oocytes; II, stage II oocytes; triangle, apoptotic oocyte; sg, spermatogonia; sc, spermatocytes. n, replicate number with similar gonads. Scale bars, 40 μm. (C) Numbers of male and female fish of each genotype, following treatment with 17α-ethynylestradiol (EE2). Fish were from the same cross as in (A).
    Ythdf3 Mutants, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ythdf3+mutants/YTHDF3+(Plasmid+%23156133)/pmc11407899-297-8-24
    Average 93 stars, based on 4 article reviews
    ythdf3 mutants - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Ythdf m 6 A Readers Function Redundantly during Zebrafish Development"

    Article Title: Ythdf m 6 A Readers Function Redundantly during Zebrafish Development

    Journal: Cell reports

    doi: 10.1016/j.celrep.2020.108598

    (A) Numbers of male and female fish of each genotype. Sibling control and double ythdf2;ythdf3 homozygotes were offspring from the same cross, depicted on top. (B) Gonad histology of double homozygous ( ythdf2 −/− ; ythdf3 −/− ) and sibling control fish from the cross in (A). At 27 dpf, mutants exhibit less developed juvenile ovaries than controls. At 35 dpf, 6 sibling fish had adult ovaries and 8 had testes, while all 12 ythdf2 −/− ; ythdf3 −/− fish had testes. I, stage I oocytes; II, stage II oocytes; triangle, apoptotic oocyte; sg, spermatogonia; sc, spermatocytes. n, replicate number with similar gonads. Scale bars, 40 μm. (C) Numbers of male and female fish of each genotype, following treatment with 17α-ethynylestradiol (EE2). Fish were from the same cross as in (A).
    Figure Legend Snippet: (A) Numbers of male and female fish of each genotype. Sibling control and double ythdf2;ythdf3 homozygotes were offspring from the same cross, depicted on top. (B) Gonad histology of double homozygous ( ythdf2 −/− ; ythdf3 −/− ) and sibling control fish from the cross in (A). At 27 dpf, mutants exhibit less developed juvenile ovaries than controls. At 35 dpf, 6 sibling fish had adult ovaries and 8 had testes, while all 12 ythdf2 −/− ; ythdf3 −/− fish had testes. I, stage I oocytes; II, stage II oocytes; triangle, apoptotic oocyte; sg, spermatogonia; sc, spermatocytes. n, replicate number with similar gonads. Scale bars, 40 μm. (C) Numbers of male and female fish of each genotype, following treatment with 17α-ethynylestradiol (EE2). Fish were from the same cross as in (A).

    Techniques Used: Control

    (A) MZ ythdf2 ;MZ ythdf3 , background-matched wild-type, and unrelated TU-AB wild-type zebrafish embryos develop at similar rates. Parents of mutant and background-matched control embryos were 17α-ethynylestradiol treated. n, replicate number of embryos at same developmental stage. Scale bars, 500 μm. (B) Biplot of expression (log 2 RPKM) of maternal (n = 13,642) and m 6 A-modified (n = 2,280) mRNAs between wild-type and MZ ythdf2 ;MZ ythdf3 , from 6 hpf poly(A) mRNA-seq. Dashed lines, 2-fold change. (C) Cumulative distribution of fold changes in maternal mRNA abundance (log 2 RPKM) between 4 and 0 hpf in MZ ythdf2 ;MZ ythdf3 embryos, for m 6 A-modified (n = 708) and non-modified (n = 841) mRNAs, from poly(A) mRNA-seq. p values computed by a Mann-Whitney U test. (D) Schematic of cross and genotyping strategy for triple Ythdf mutants. Female fish ( ythdf1 +/− ; ythdf2 −/− ; ythdf3 +/− ) were crossed to males ( ythdf1 −/− ; ythdf -+/− ; ythdf3 −/− ) to generate triple homozygotes (1 of 8 possible genotypes). Every 3 days, 48 larvae were genotyped, with 200 more fish genotyped at 30 dpf. (E) Percentage of triple heterozygous (het) or triple homozygous (homo) fish during development. Dotted line, expected percentage (12.5%) of each genotype, from cross in (D). (F) Number of fish with each genotype from cross in (D) at 30 dpf. For each ythdf allele: filled circle, heterozygous; m, homozygous. Dotted line, expected fish number (25), equal for all genotypes.
    Figure Legend Snippet: (A) MZ ythdf2 ;MZ ythdf3 , background-matched wild-type, and unrelated TU-AB wild-type zebrafish embryos develop at similar rates. Parents of mutant and background-matched control embryos were 17α-ethynylestradiol treated. n, replicate number of embryos at same developmental stage. Scale bars, 500 μm. (B) Biplot of expression (log 2 RPKM) of maternal (n = 13,642) and m 6 A-modified (n = 2,280) mRNAs between wild-type and MZ ythdf2 ;MZ ythdf3 , from 6 hpf poly(A) mRNA-seq. Dashed lines, 2-fold change. (C) Cumulative distribution of fold changes in maternal mRNA abundance (log 2 RPKM) between 4 and 0 hpf in MZ ythdf2 ;MZ ythdf3 embryos, for m 6 A-modified (n = 708) and non-modified (n = 841) mRNAs, from poly(A) mRNA-seq. p values computed by a Mann-Whitney U test. (D) Schematic of cross and genotyping strategy for triple Ythdf mutants. Female fish ( ythdf1 +/− ; ythdf2 −/− ; ythdf3 +/− ) were crossed to males ( ythdf1 −/− ; ythdf -+/− ; ythdf3 −/− ) to generate triple homozygotes (1 of 8 possible genotypes). Every 3 days, 48 larvae were genotyped, with 200 more fish genotyped at 30 dpf. (E) Percentage of triple heterozygous (het) or triple homozygous (homo) fish during development. Dotted line, expected percentage (12.5%) of each genotype, from cross in (D). (F) Number of fish with each genotype from cross in (D) at 30 dpf. For each ythdf allele: filled circle, heterozygous; m, homozygous. Dotted line, expected fish number (25), equal for all genotypes.

    Techniques Used: Mutagenesis, Control, Expressing, Modification, MANN-WHITNEY

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, Nick Translation, SYBR Green Assay, RNA Extraction, Reverse Transcription, Mutagenesis, Software

    Related Articles

    Recombinant:

    Article Title: Ythdf m 6 A Readers Function Redundantly during Zebrafish Development.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit anti-Ythdf1 this manuscript Ynzyme custom order rabbit anti-Ythdf2 this manuscript Ynzyme custom order rabbit anti-Ythdf3 this manuscript Ynzyme custom order rabbit anti-Actin Abcam Cat# ab8227; RRID: AB_2305186 goat anti-rabbit IgG, (H+L) HRP conjugate Millipore Cat# AP307P; RRID: AB_92641 Chemicals, Peptides, and Recombinant Proteins triptolide Sigma-aldrich Cat# T3652 a-amanitin Sigma-aldrich Cat# A2263 low melt agarose AmericanBio Cat# CAS: 9012-36-6 N6-methyladenosine 5’-triphosphate TriLink Cat# N-1013-5 m7G(50)ppp(50)G RNA cap structure analog New England BioLabs Cat# S1404S 17 a-ethynylestradiol (EE2) Sigma-Aldrich Cat# E4876 Critical Commercial Assays AmpliScribe-T7-Flash Transcription kit Epicenter Cat# ASF3257 HiScribe SP6 RNA Synthesis Kit New England BioLabs Cat# E2070S mMessage mMachine SP6 Transcription Kit Invitrogen Cat# 74104 Nick Translation Kit Sigma-Aldrich Cat# 10976776001 Poly(A) tailing kit Invitrogen Cat# AM1350 Power SYBR Green PCR Master Mix Applied Biosystems Cat# 4368706 Protein A Dynabeads Invitrogen Cat# 10008D RNeasy RNA extraction kit QIAGEN Cat# AM1340 Superscript III Reverse Transcriptase kit Invitrogen Cat# 18080093 TRIzol reagent Invitrogen Cat# 15596-018 Deposited Data mRNA-sequencing of MZythdf2, MZythdf2;MZythdf3, or MZdicer mutants this manuscript SRP297464 mRNA-sequencing of MZythdf2 mutants Zhao et al., 2017 GSE79213 mRNA-sequencing time course of zebrafish embryos Vejnar et al., 2019 Beaudoin et al., 2018 Bazzini et al., 2016 SRP189512 SRP149556 SRP072296 PAL-sequencing in zebrafish embryos Subtelny et al., 2014 GSE52809 TAIL-sequencing in zebrafish embryos Chang et al., 2018 https://doi.org/10.5281/zenodo.2640028 Experimental Models: Organisms/Strains Zebrafish: TU-AB and TLF strains Zebrafish International Resource Center N/A Zebrafish: miR-430 cluster deletion mutant Liu et al., 2013 N/A Zebrafish: ythdf2 8bp mutants Zhao et al., 2017 N/A Zebrafish: dicer mutants Giraldez et al., 2006 N/A Zebrafish: mettl3 mutants this manuscript N/A Zebrafish: mettl14 mutants this manuscript N/A Zebrafish: ythdf1 mutants this manuscript N/A Zebrafish: ythdf2 223bp mutants this manuscript N/A (Continued on next page) Cell Reports 33, 108598, December 29, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Zebrafish: ythdf3 mutants this manuscript N/A Zebrafish: ythdf2;ythdf3 double mutants this manuscript N/A Zebrafish: ythdf1;ythdf2;ythdf3 triple mutants this manuscript N/A Oligonucleotides See Table S3 for primers and oligonucleotides used in this manuscript Recombinant DNA pSP64T-ythdf1-3xflag this manuscript Addgene #164488 pSP64T-ythdf2-3xflag this manuscript Addgene #164489 pSP64T-ythdf3-3xflag this manuscript Addgene #164490 pCS2+methylated-reporter this manuscript Addgene #164491 pT3TS-nCas9n Jao et al., 2013 Addgene #46757 Software and Algorithms STAR Dobin et al., 2013 https://github.com/alexdobin/STAR Python (data analysis) Python 3.8 https://www.python.org DESeq2 Love et al., 2014 https://bioc.ism.ac.jp/packages/3.1/bioc/ html/DESeq2.html ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ SnapGene GSL Biotech snapgene.com CRISPRscan Moreno-Mateos et al., 2015 crisprscan.org ..

    Software:

    Article Title: Ythdf m 6 A Readers Function Redundantly during Zebrafish Development.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit anti-Ythdf1 this manuscript Ynzyme custom order rabbit anti-Ythdf2 this manuscript Ynzyme custom order rabbit anti-Ythdf3 this manuscript Ynzyme custom order rabbit anti-Actin Abcam Cat# ab8227; RRID: AB_2305186 goat anti-rabbit IgG, (H+L) HRP conjugate Millipore Cat# AP307P; RRID: AB_92641 Chemicals, Peptides, and Recombinant Proteins triptolide Sigma-aldrich Cat# T3652 a-amanitin Sigma-aldrich Cat# A2263 low melt agarose AmericanBio Cat# CAS: 9012-36-6 N6-methyladenosine 5’-triphosphate TriLink Cat# N-1013-5 m7G(50)ppp(50)G RNA cap structure analog New England BioLabs Cat# S1404S 17 a-ethynylestradiol (EE2) Sigma-Aldrich Cat# E4876 Critical Commercial Assays AmpliScribe-T7-Flash Transcription kit Epicenter Cat# ASF3257 HiScribe SP6 RNA Synthesis Kit New England BioLabs Cat# E2070S mMessage mMachine SP6 Transcription Kit Invitrogen Cat# 74104 Nick Translation Kit Sigma-Aldrich Cat# 10976776001 Poly(A) tailing kit Invitrogen Cat# AM1350 Power SYBR Green PCR Master Mix Applied Biosystems Cat# 4368706 Protein A Dynabeads Invitrogen Cat# 10008D RNeasy RNA extraction kit QIAGEN Cat# AM1340 Superscript III Reverse Transcriptase kit Invitrogen Cat# 18080093 TRIzol reagent Invitrogen Cat# 15596-018 Deposited Data mRNA-sequencing of MZythdf2, MZythdf2;MZythdf3, or MZdicer mutants this manuscript SRP297464 mRNA-sequencing of MZythdf2 mutants Zhao et al., 2017 GSE79213 mRNA-sequencing time course of zebrafish embryos Vejnar et al., 2019 Beaudoin et al., 2018 Bazzini et al., 2016 SRP189512 SRP149556 SRP072296 PAL-sequencing in zebrafish embryos Subtelny et al., 2014 GSE52809 TAIL-sequencing in zebrafish embryos Chang et al., 2018 https://doi.org/10.5281/zenodo.2640028 Experimental Models: Organisms/Strains Zebrafish: TU-AB and TLF strains Zebrafish International Resource Center N/A Zebrafish: miR-430 cluster deletion mutant Liu et al., 2013 N/A Zebrafish: ythdf2 8bp mutants Zhao et al., 2017 N/A Zebrafish: dicer mutants Giraldez et al., 2006 N/A Zebrafish: mettl3 mutants this manuscript N/A Zebrafish: mettl14 mutants this manuscript N/A Zebrafish: ythdf1 mutants this manuscript N/A Zebrafish: ythdf2 223bp mutants this manuscript N/A (Continued on next page) Cell Reports 33, 108598, December 29, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Zebrafish: ythdf3 mutants this manuscript N/A Zebrafish: ythdf2;ythdf3 double mutants this manuscript N/A Zebrafish: ythdf1;ythdf2;ythdf3 triple mutants this manuscript N/A Oligonucleotides See Table S3 for primers and oligonucleotides used in this manuscript Recombinant DNA pSP64T-ythdf1-3xflag this manuscript Addgene #164488 pSP64T-ythdf2-3xflag this manuscript Addgene #164489 pSP64T-ythdf3-3xflag this manuscript Addgene #164490 pCS2+methylated-reporter this manuscript Addgene #164491 pT3TS-nCas9n Jao et al., 2013 Addgene #46757 Software and Algorithms STAR Dobin et al., 2013 https://github.com/alexdobin/STAR Python (data analysis) Python 3.8 https://www.python.org DESeq2 Love et al., 2014 https://bioc.ism.ac.jp/packages/3.1/bioc/ html/DESeq2.html ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ SnapGene GSL Biotech snapgene.com CRISPRscan Moreno-Mateos et al., 2015 crisprscan.org ..

    Plasmid Preparation:

    Article Title: Ythdf m 6 A Readers Function Redundantly during Zebrafish Development
    Article Snippet: After PCR amplification, products were purified using QIAquick PCR purification kit (QIAGEN, 28104). sgRNAs were synthesized from the purified PCR product by T7 IVT using an AmpliScribe-T7-Flash Transcription kit (Epicenter, ASF3257), reaction at 37°C for 6 h). sgRNAs were DNase-treated, purified by sodium acetate and ethanol precipitation, and checked for RNA integrity by agarose gel electrophoresis. .. For gene editing to generate ythdf2 −223/−223 and ythdf3 mutants, 30 pg of each sgRNA was co-injected with 150 pg of Cas9 (plasmid pT3TS-nCas9n, Addgene #46757, ( )) capped mRNA synthesized using mMessage mMachine T3 Transcription kit (Thermo Fisher Scientific, AM1340). ..

    Synthesized:

    Article Title: Ythdf m 6 A Readers Function Redundantly during Zebrafish Development
    Article Snippet: After PCR amplification, products were purified using QIAquick PCR purification kit (QIAGEN, 28104). sgRNAs were synthesized from the purified PCR product by T7 IVT using an AmpliScribe-T7-Flash Transcription kit (Epicenter, ASF3257), reaction at 37°C for 6 h). sgRNAs were DNase-treated, purified by sodium acetate and ethanol precipitation, and checked for RNA integrity by agarose gel electrophoresis. .. For gene editing to generate ythdf2 −223/−223 and ythdf3 mutants, 30 pg of each sgRNA was co-injected with 150 pg of Cas9 (plasmid pT3TS-nCas9n, Addgene #46757, ( )) capped mRNA synthesized using mMessage mMachine T3 Transcription kit (Thermo Fisher Scientific, AM1340). ..



    Similar Products

    ythdf3 mutants  (Addgene inc)
    93
    Addgene inc ythdf3 mutants
    (A) Numbers of male and female fish of each genotype. Sibling control and double <t>ythdf2;ythdf3</t> homozygotes were offspring from the same cross, depicted on top. (B) Gonad histology of double homozygous ( ythdf2 −/− ; ythdf3 −/− ) and sibling control fish from the cross in (A). At 27 dpf, mutants exhibit less developed juvenile ovaries than controls. At 35 dpf, 6 sibling fish had adult ovaries and 8 had testes, while all 12 ythdf2 −/− ; ythdf3 −/− fish had testes. I, stage I oocytes; II, stage II oocytes; triangle, apoptotic oocyte; sg, spermatogonia; sc, spermatocytes. n, replicate number with similar gonads. Scale bars, 40 μm. (C) Numbers of male and female fish of each genotype, following treatment with 17α-ethynylestradiol (EE2). Fish were from the same cross as in (A).
    Ythdf3 Mutants, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ythdf3+mutants/YTHDF3+(Plasmid+%23156133)/pmc11407899-297-8-24
    Average 93 stars, based on 1 article reviews
    ythdf3 mutants - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Numbers of male and female fish of each genotype. Sibling control and double ythdf2;ythdf3 homozygotes were offspring from the same cross, depicted on top. (B) Gonad histology of double homozygous ( ythdf2 −/− ; ythdf3 −/− ) and sibling control fish from the cross in (A). At 27 dpf, mutants exhibit less developed juvenile ovaries than controls. At 35 dpf, 6 sibling fish had adult ovaries and 8 had testes, while all 12 ythdf2 −/− ; ythdf3 −/− fish had testes. I, stage I oocytes; II, stage II oocytes; triangle, apoptotic oocyte; sg, spermatogonia; sc, spermatocytes. n, replicate number with similar gonads. Scale bars, 40 μm. (C) Numbers of male and female fish of each genotype, following treatment with 17α-ethynylestradiol (EE2). Fish were from the same cross as in (A).

    Journal: Cell reports

    Article Title: Ythdf m 6 A Readers Function Redundantly during Zebrafish Development

    doi: 10.1016/j.celrep.2020.108598

    Figure Lengend Snippet: (A) Numbers of male and female fish of each genotype. Sibling control and double ythdf2;ythdf3 homozygotes were offspring from the same cross, depicted on top. (B) Gonad histology of double homozygous ( ythdf2 −/− ; ythdf3 −/− ) and sibling control fish from the cross in (A). At 27 dpf, mutants exhibit less developed juvenile ovaries than controls. At 35 dpf, 6 sibling fish had adult ovaries and 8 had testes, while all 12 ythdf2 −/− ; ythdf3 −/− fish had testes. I, stage I oocytes; II, stage II oocytes; triangle, apoptotic oocyte; sg, spermatogonia; sc, spermatocytes. n, replicate number with similar gonads. Scale bars, 40 μm. (C) Numbers of male and female fish of each genotype, following treatment with 17α-ethynylestradiol (EE2). Fish were from the same cross as in (A).

    Article Snippet: For gene editing to generate ythdf2 −223/−223 and ythdf3 mutants, 30 pg of each sgRNA was co-injected with 150 pg of Cas9 (plasmid pT3TS-nCas9n, Addgene #46757, ( )) capped mRNA synthesized using mMessage mMachine T3 Transcription kit (Thermo Fisher Scientific, AM1340).

    Techniques: Control

    (A) MZ ythdf2 ;MZ ythdf3 , background-matched wild-type, and unrelated TU-AB wild-type zebrafish embryos develop at similar rates. Parents of mutant and background-matched control embryos were 17α-ethynylestradiol treated. n, replicate number of embryos at same developmental stage. Scale bars, 500 μm. (B) Biplot of expression (log 2 RPKM) of maternal (n = 13,642) and m 6 A-modified (n = 2,280) mRNAs between wild-type and MZ ythdf2 ;MZ ythdf3 , from 6 hpf poly(A) mRNA-seq. Dashed lines, 2-fold change. (C) Cumulative distribution of fold changes in maternal mRNA abundance (log 2 RPKM) between 4 and 0 hpf in MZ ythdf2 ;MZ ythdf3 embryos, for m 6 A-modified (n = 708) and non-modified (n = 841) mRNAs, from poly(A) mRNA-seq. p values computed by a Mann-Whitney U test. (D) Schematic of cross and genotyping strategy for triple Ythdf mutants. Female fish ( ythdf1 +/− ; ythdf2 −/− ; ythdf3 +/− ) were crossed to males ( ythdf1 −/− ; ythdf -+/− ; ythdf3 −/− ) to generate triple homozygotes (1 of 8 possible genotypes). Every 3 days, 48 larvae were genotyped, with 200 more fish genotyped at 30 dpf. (E) Percentage of triple heterozygous (het) or triple homozygous (homo) fish during development. Dotted line, expected percentage (12.5%) of each genotype, from cross in (D). (F) Number of fish with each genotype from cross in (D) at 30 dpf. For each ythdf allele: filled circle, heterozygous; m, homozygous. Dotted line, expected fish number (25), equal for all genotypes.

    Journal: Cell reports

    Article Title: Ythdf m 6 A Readers Function Redundantly during Zebrafish Development

    doi: 10.1016/j.celrep.2020.108598

    Figure Lengend Snippet: (A) MZ ythdf2 ;MZ ythdf3 , background-matched wild-type, and unrelated TU-AB wild-type zebrafish embryos develop at similar rates. Parents of mutant and background-matched control embryos were 17α-ethynylestradiol treated. n, replicate number of embryos at same developmental stage. Scale bars, 500 μm. (B) Biplot of expression (log 2 RPKM) of maternal (n = 13,642) and m 6 A-modified (n = 2,280) mRNAs between wild-type and MZ ythdf2 ;MZ ythdf3 , from 6 hpf poly(A) mRNA-seq. Dashed lines, 2-fold change. (C) Cumulative distribution of fold changes in maternal mRNA abundance (log 2 RPKM) between 4 and 0 hpf in MZ ythdf2 ;MZ ythdf3 embryos, for m 6 A-modified (n = 708) and non-modified (n = 841) mRNAs, from poly(A) mRNA-seq. p values computed by a Mann-Whitney U test. (D) Schematic of cross and genotyping strategy for triple Ythdf mutants. Female fish ( ythdf1 +/− ; ythdf2 −/− ; ythdf3 +/− ) were crossed to males ( ythdf1 −/− ; ythdf -+/− ; ythdf3 −/− ) to generate triple homozygotes (1 of 8 possible genotypes). Every 3 days, 48 larvae were genotyped, with 200 more fish genotyped at 30 dpf. (E) Percentage of triple heterozygous (het) or triple homozygous (homo) fish during development. Dotted line, expected percentage (12.5%) of each genotype, from cross in (D). (F) Number of fish with each genotype from cross in (D) at 30 dpf. For each ythdf allele: filled circle, heterozygous; m, homozygous. Dotted line, expected fish number (25), equal for all genotypes.

    Article Snippet: For gene editing to generate ythdf2 −223/−223 and ythdf3 mutants, 30 pg of each sgRNA was co-injected with 150 pg of Cas9 (plasmid pT3TS-nCas9n, Addgene #46757, ( )) capped mRNA synthesized using mMessage mMachine T3 Transcription kit (Thermo Fisher Scientific, AM1340).

    Techniques: Mutagenesis, Control, Expressing, Modification, MANN-WHITNEY

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Ythdf m 6 A Readers Function Redundantly during Zebrafish Development

    doi: 10.1016/j.celrep.2020.108598

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: For gene editing to generate ythdf2 −223/−223 and ythdf3 mutants, 30 pg of each sgRNA was co-injected with 150 pg of Cas9 (plasmid pT3TS-nCas9n, Addgene #46757, ( )) capped mRNA synthesized using mMessage mMachine T3 Transcription kit (Thermo Fisher Scientific, AM1340).

    Techniques: Recombinant, Nick Translation, SYBR Green Assay, RNA Extraction, Reverse Transcription, Mutagenesis, Software