yo pro 1 pi (Beyotime)
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Yo Pro 1 Pi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 161 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yo+pro+1/YO-PRO-1/pmc13010924-111-6-8
Average 99 stars, based on 161 article reviews
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Membrane:Article Title: Biomimetic nanoimmunotherapy boosts spatiotemporal PANoptosis and reshapes desmoplastic tumor microenvironment Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER (S)-Crizotinib Aladdin Cat#S288194 Tetrakis(4-carboxyphenyl)porphyrin (TCPP) TCI Cat#A5015 1-octadecene Sigma-Aldrich Cat#O806 Oleic acid Sigma-Aldrich Cat#364525 Polyethylene glycol (PEG) solution (50% (w/v)) Sigma-Aldrich Cat#P7306 Tetrahydrobiopterin (BH4, 11.212) Schircks Labs N/A Critical commercial assays Membrane and Cytosol Protein Extraction Kit Beyotime Cat#P0033 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033 Alexa Fluor 488–conjugated avidin Thermo Fisher Cat#S11223 Cell Counting Kit-8 Beyotime Cat#C0038 Calcein/PI Cell Viability/Cytotoxicity Assay Kit Beyotime Cat#C2015 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062 Apoptosis and Necrosis Detection Kit with Protein Extraction:Article Title: Biomimetic nanoimmunotherapy boosts spatiotemporal PANoptosis and reshapes desmoplastic tumor microenvironment Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER (S)-Crizotinib Aladdin Cat#S288194 Tetrakis(4-carboxyphenyl)porphyrin (TCPP) TCI Cat#A5015 1-octadecene Sigma-Aldrich Cat#O806 Oleic acid Sigma-Aldrich Cat#364525 Polyethylene glycol (PEG) solution (50% (w/v)) Sigma-Aldrich Cat#P7306 Tetrahydrobiopterin (BH4, 11.212) Schircks Labs N/A Critical commercial assays Membrane and Cytosol Protein Extraction Kit Beyotime Cat#P0033 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033 Alexa Fluor 488–conjugated avidin Thermo Fisher Cat#S11223 Cell Counting Kit-8 Beyotime Cat#C0038 Calcein/PI Cell Viability/Cytotoxicity Assay Kit Beyotime Cat#C2015 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062 Apoptosis and Necrosis Detection Kit with Avidin-Biotin Assay:Article Title: Biomimetic nanoimmunotherapy boosts spatiotemporal PANoptosis and reshapes desmoplastic tumor microenvironment Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER (S)-Crizotinib Aladdin Cat#S288194 Tetrakis(4-carboxyphenyl)porphyrin (TCPP) TCI Cat#A5015 1-octadecene Sigma-Aldrich Cat#O806 Oleic acid Sigma-Aldrich Cat#364525 Polyethylene glycol (PEG) solution (50% (w/v)) Sigma-Aldrich Cat#P7306 Tetrahydrobiopterin (BH4, 11.212) Schircks Labs N/A Critical commercial assays Membrane and Cytosol Protein Extraction Kit Beyotime Cat#P0033 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033 Alexa Fluor 488–conjugated avidin Thermo Fisher Cat#S11223 Cell Counting Kit-8 Beyotime Cat#C0038 Calcein/PI Cell Viability/Cytotoxicity Assay Kit Beyotime Cat#C2015 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062 Apoptosis and Necrosis Detection Kit with CCK-8 Assay:Article Title: Biomimetic nanoimmunotherapy boosts spatiotemporal PANoptosis and reshapes desmoplastic tumor microenvironment Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER (S)-Crizotinib Aladdin Cat#S288194 Tetrakis(4-carboxyphenyl)porphyrin (TCPP) TCI Cat#A5015 1-octadecene Sigma-Aldrich Cat#O806 Oleic acid Sigma-Aldrich Cat#364525 Polyethylene glycol (PEG) solution (50% (w/v)) Sigma-Aldrich Cat#P7306 Tetrahydrobiopterin (BH4, 11.212) Schircks Labs N/A Critical commercial assays Membrane and Cytosol Protein Extraction Kit Beyotime Cat#P0033 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033 Alexa Fluor 488–conjugated avidin Thermo Fisher Cat#S11223 Cell Counting Kit-8 Beyotime Cat#C0038 Calcein/PI Cell Viability/Cytotoxicity Assay Kit Beyotime Cat#C2015 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062 Apoptosis and Necrosis Detection Kit with LDH Cytotoxicity Assay:Article Title: Biomimetic nanoimmunotherapy boosts spatiotemporal PANoptosis and reshapes desmoplastic tumor microenvironment Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER (S)-Crizotinib Aladdin Cat#S288194 Tetrakis(4-carboxyphenyl)porphyrin (TCPP) TCI Cat#A5015 1-octadecene Sigma-Aldrich Cat#O806 Oleic acid Sigma-Aldrich Cat#364525 Polyethylene glycol (PEG) solution (50% (w/v)) Sigma-Aldrich Cat#P7306 Tetrahydrobiopterin (BH4, 11.212) Schircks Labs N/A Critical commercial assays Membrane and Cytosol Protein Extraction Kit Beyotime Cat#P0033 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033 Alexa Fluor 488–conjugated avidin Thermo Fisher Cat#S11223 Cell Counting Kit-8 Beyotime Cat#C0038 Calcein/PI Cell Viability/Cytotoxicity Assay Kit Beyotime Cat#C2015 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062 Apoptosis and Necrosis Detection Kit with Article Title: Targeting ROS-metabolism dual pathways to trigger PANoptosis by cobalt-vanadium oxides biomimetic nanocakes for tumor immunotherapy. Article Snippet: To overcome the resistance of tumor cells to death through a single pathway, PANoptosis, a novel synergistic mode of cell death, can simultaneously activates pyroptosis, apoptosis, and necroptosis.. Most current strategies for inducing PANoptosis focused on amplifying reactive oxygen species (ROS) through exogenous stimuli, while the regulatory role of tumor cell metabolic characteristic in this process has long been overlooked.. We propose a ROS-Metabolism dual axis synergistic induction of PANoptosis, simultaneously enhancing antitumor immune responses, and construct a novel bionic nanoplatform named CVOVM. Enzyme-linked Immunosorbent Assay:Article Title: Biomimetic nanoimmunotherapy boosts spatiotemporal PANoptosis and reshapes desmoplastic tumor microenvironment Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER (S)-Crizotinib Aladdin Cat#S288194 Tetrakis(4-carboxyphenyl)porphyrin (TCPP) TCI Cat#A5015 1-octadecene Sigma-Aldrich Cat#O806 Oleic acid Sigma-Aldrich Cat#364525 Polyethylene glycol (PEG) solution (50% (w/v)) Sigma-Aldrich Cat#P7306 Tetrahydrobiopterin (BH4, 11.212) Schircks Labs N/A Critical commercial assays Membrane and Cytosol Protein Extraction Kit Beyotime Cat#P0033 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033 Alexa Fluor 488–conjugated avidin Thermo Fisher Cat#S11223 Cell Counting Kit-8 Beyotime Cat#C0038 Calcein/PI Cell Viability/Cytotoxicity Assay Kit Beyotime Cat#C2015 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062 Apoptosis and Necrosis Detection Kit with RNA Sequencing:Article Title: Biomimetic nanoimmunotherapy boosts spatiotemporal PANoptosis and reshapes desmoplastic tumor microenvironment Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER (S)-Crizotinib Aladdin Cat#S288194 Tetrakis(4-carboxyphenyl)porphyrin (TCPP) TCI Cat#A5015 1-octadecene Sigma-Aldrich Cat#O806 Oleic acid Sigma-Aldrich Cat#364525 Polyethylene glycol (PEG) solution (50% (w/v)) Sigma-Aldrich Cat#P7306 Tetrahydrobiopterin (BH4, 11.212) Schircks Labs N/A Critical commercial assays Membrane and Cytosol Protein Extraction Kit Beyotime Cat#P0033 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033 Alexa Fluor 488–conjugated avidin Thermo Fisher Cat#S11223 Cell Counting Kit-8 Beyotime Cat#C0038 Calcein/PI Cell Viability/Cytotoxicity Assay Kit Beyotime Cat#C2015 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062 Apoptosis and Necrosis Detection Kit with Software:Article Title: Biomimetic nanoimmunotherapy boosts spatiotemporal PANoptosis and reshapes desmoplastic tumor microenvironment Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER (S)-Crizotinib Aladdin Cat#S288194 Tetrakis(4-carboxyphenyl)porphyrin (TCPP) TCI Cat#A5015 1-octadecene Sigma-Aldrich Cat#O806 Oleic acid Sigma-Aldrich Cat#364525 Polyethylene glycol (PEG) solution (50% (w/v)) Sigma-Aldrich Cat#P7306 Tetrahydrobiopterin (BH4, 11.212) Schircks Labs N/A Critical commercial assays Membrane and Cytosol Protein Extraction Kit Beyotime Cat#P0033 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033 Alexa Fluor 488–conjugated avidin Thermo Fisher Cat#S11223 Cell Counting Kit-8 Beyotime Cat#C0038 Calcein/PI Cell Viability/Cytotoxicity Assay Kit Beyotime Cat#C2015 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062 Apoptosis and Necrosis Detection Kit with Double Staining:Article Title: Transferrin receptor serves a role in promoting PANoptosis in thyroid cancer Article Snippet: Transfections were performed using Lipo8000 TM transfection reagent (cat. no. C0533-7.5 ml; Beyotime Institute of Biotechnology), with BCPAP cells transfected with TFRC overexpression plasmid or its NC (blank PGMLV-CMV-MCS-EF1-ZsGreen1-T2A-Puro plasmid) and K1 cells transfected with TFRC shRNA or its NC (oligo control). .. The PI/Calcein-AM Double Staining Kit (cat. no. CA1630; Beijing Solarbio Science & Technology Co., Ltd.) and Apoptosis and Necrosis Detection Kit with Concentration Assay:Article Title: Emestrin‐Type Epidithiodiketopiperazines Inhibited Gasdermin D‐Mediated Pyroptosis via Caspase‐3/7 Activation Article Snippet: The starved cells were then primed with 1 μg/mL LPS (Sigma; L4391) and 3 mM ATP (Sigma; A2383), and supplemented with either vehicle or the indicated compounds for 3 h. Subsequently, the cells were treated with 10 μM nigericin (Selleck; S6653) for 1 h. Both the cells and culture medium were collected for further analysis. .. For HCS, THP‐1 cells were differentiated with PMA and seeded into 96‐well plates at a density of 6 × 10 4 cells/well for 48 h. Prior to treatment, the cells were starved in RPMI 1640 medium for 24 h. Starved cells were then primed with 1 μg/mL LPS and 3 mM ATP, each supplemented with one of 536 different compounds in separate wells at a final concentration of 1 μM for 3 h. Following this, cells were treated with 10 μM Nigericin and 1 μM Labeling:Article Title: Targeting HIF-1α rescues microglial efferocytosis via the SLC7A11-TAM pathway to ameliorate Sepsis-associated encephalopathy. Article Snippet: Sepsis-associated encephalopathy (SAE) is a common and debilitating complication of sepsis, yet its cellular mechanisms and targeted therapies remain unclear.. Microglia preserve neuroinflammatory homeostasis and neural circuit integrity through efferocytosis, but how this process is altered in SAE and regulated by immunometabolism is poorly defined.. Here, we investigated the molecular basis of microglial efferocytosis impairment using LPS-stimulated BV2 cells and a cecal ligation and puncture (CLP) murine SAE model. We integrated RNA sequencing, HIF-1α and SLC7A11 gainand loss-of-function approaches and in vitro functional assays. ATP Assay:Article Title: Targeting ROS-metabolism dual pathways to trigger PANoptosis by cobalt-vanadium oxides biomimetic nanocakes for tumor immunotherapy. Article Snippet: To overcome the resistance of tumor cells to death through a single pathway, PANoptosis, a novel synergistic mode of cell death, can simultaneously activates pyroptosis, apoptosis, and necroptosis.. Most current strategies for inducing PANoptosis focused on amplifying reactive oxygen species (ROS) through exogenous stimuli, while the regulatory role of tumor cell metabolic characteristic in this process has long been overlooked.. We propose a ROS-Metabolism dual axis synergistic induction of PANoptosis, simultaneously enhancing antitumor immune responses, and construct a novel bionic nanoplatform named CVOVM. other:Article Title: A biomimetic arsenic-based nanozyme enhances dual epigenetic regulation to improve the efficacy of immunotherapy for acute myeloid leukaemia. Article Snippet: Aberrant epigenetic modifications in acute myeloid leukaemia (AML) limit immunotherapeutic efficacy.. Arsenic ions are capable of undergoing valence transformation in organisms, exhibit favourable enzyme-mimetic activity, and thereby play a crucial role in enhancing dual epigenetic regulation.. Therefore, to achieve potent epigenetic regulation, in this study, a biomimetic arsenic-based nanozyme (As/ZIF-8@M) that exhibits leukaemia cell recognition and phagocytosis capabilities, as well as bone marrow homing effects, was engineered. |
