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    Beyotime yo pro 1 pi
    Yo Pro 1 Pi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 161 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/yo+pro+1/YO-PRO-1/pmc13010924-111-6-8
    Average 99 stars, based on 161 article reviews
    yo pro 1 pi - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Membrane:

    Article Title: Biomimetic nanoimmunotherapy boosts spatiotemporal PANoptosis and reshapes desmoplastic tumor microenvironment
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER (S)-Crizotinib Aladdin Cat#S288194 Tetrakis(4-carboxyphenyl)porphyrin (TCPP) TCI Cat#A5015 1-octadecene Sigma-Aldrich Cat#O806 Oleic acid Sigma-Aldrich Cat#364525 Polyethylene glycol (PEG) solution (50% (w/v)) Sigma-Aldrich Cat#P7306 Tetrahydrobiopterin (BH4, 11.212) Schircks Labs N/A Critical commercial assays Membrane and Cytosol Protein Extraction Kit Beyotime Cat#P0033 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033 Alexa Fluor 488–conjugated avidin Thermo Fisher Cat#S11223 Cell Counting Kit-8 Beyotime Cat#C0038 Calcein/PI Cell Viability/Cytotoxicity Assay Kit Beyotime Cat#C2015 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062 Apoptosis and Necrosis Detection Kit with YO-PRO-1 and PI Beyotime Cat#C1075 LDH Cytotoxicity Assay Kit Beyotime Cat#C0017 Mouse IL-1β ELISA Kit YUBIBIO Cat#U96-1494E Mouse IL-18 ELISA Kit YUBIBIO Cat#U96-3279E Deposited data Bulk RNA sequencing data This paper [NCBI]: [PRJNA1286157] Experimental models: Cell lines Murine 4T1 breast cancer cell line ATCC Cat#CRL-2539 Murine B16F10 melanoma cell line ATCC Cat#CRL-6475 Experimental models: Organisms/strains Mouse: Female BALB/c Shanghai Model Organisms Center N/A Mouse: Female C57BL/6J Shanghai Model Organisms Center N/A Mouse: Female BALB/c-nu Shanghai Model Organisms Center N/A Software and algorithms GraphPad Prism (version 9.5.1) GraphPad Software https://www.graphpad.com/ Adobe Illustrator Adobe https://www.adobe.com/products/ illustrator/free-trial-download.html ZEN Zeiss https://www.zeiss.com/microscopy/ en/products/software/zeiss-zen.html FlowJo (version 10.8.1) BD Biosciences https://www.flowjo.com/ Living Image (version 4.1) Caliper Life Sciences https://living-image.software. informer.com/4.1/ e2 Cell Reports Medicine 6, 102312, September 16, 2025 Article ll OPEN ACCESS ..

    Protein Extraction:

    Article Title: Biomimetic nanoimmunotherapy boosts spatiotemporal PANoptosis and reshapes desmoplastic tumor microenvironment
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER (S)-Crizotinib Aladdin Cat#S288194 Tetrakis(4-carboxyphenyl)porphyrin (TCPP) TCI Cat#A5015 1-octadecene Sigma-Aldrich Cat#O806 Oleic acid Sigma-Aldrich Cat#364525 Polyethylene glycol (PEG) solution (50% (w/v)) Sigma-Aldrich Cat#P7306 Tetrahydrobiopterin (BH4, 11.212) Schircks Labs N/A Critical commercial assays Membrane and Cytosol Protein Extraction Kit Beyotime Cat#P0033 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033 Alexa Fluor 488–conjugated avidin Thermo Fisher Cat#S11223 Cell Counting Kit-8 Beyotime Cat#C0038 Calcein/PI Cell Viability/Cytotoxicity Assay Kit Beyotime Cat#C2015 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062 Apoptosis and Necrosis Detection Kit with YO-PRO-1 and PI Beyotime Cat#C1075 LDH Cytotoxicity Assay Kit Beyotime Cat#C0017 Mouse IL-1β ELISA Kit YUBIBIO Cat#U96-1494E Mouse IL-18 ELISA Kit YUBIBIO Cat#U96-3279E Deposited data Bulk RNA sequencing data This paper [NCBI]: [PRJNA1286157] Experimental models: Cell lines Murine 4T1 breast cancer cell line ATCC Cat#CRL-2539 Murine B16F10 melanoma cell line ATCC Cat#CRL-6475 Experimental models: Organisms/strains Mouse: Female BALB/c Shanghai Model Organisms Center N/A Mouse: Female C57BL/6J Shanghai Model Organisms Center N/A Mouse: Female BALB/c-nu Shanghai Model Organisms Center N/A Software and algorithms GraphPad Prism (version 9.5.1) GraphPad Software https://www.graphpad.com/ Adobe Illustrator Adobe https://www.adobe.com/products/ illustrator/free-trial-download.html ZEN Zeiss https://www.zeiss.com/microscopy/ en/products/software/zeiss-zen.html FlowJo (version 10.8.1) BD Biosciences https://www.flowjo.com/ Living Image (version 4.1) Caliper Life Sciences https://living-image.software. informer.com/4.1/ e2 Cell Reports Medicine 6, 102312, September 16, 2025 Article ll OPEN ACCESS ..

    Avidin-Biotin Assay:

    Article Title: Biomimetic nanoimmunotherapy boosts spatiotemporal PANoptosis and reshapes desmoplastic tumor microenvironment
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER (S)-Crizotinib Aladdin Cat#S288194 Tetrakis(4-carboxyphenyl)porphyrin (TCPP) TCI Cat#A5015 1-octadecene Sigma-Aldrich Cat#O806 Oleic acid Sigma-Aldrich Cat#364525 Polyethylene glycol (PEG) solution (50% (w/v)) Sigma-Aldrich Cat#P7306 Tetrahydrobiopterin (BH4, 11.212) Schircks Labs N/A Critical commercial assays Membrane and Cytosol Protein Extraction Kit Beyotime Cat#P0033 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033 Alexa Fluor 488–conjugated avidin Thermo Fisher Cat#S11223 Cell Counting Kit-8 Beyotime Cat#C0038 Calcein/PI Cell Viability/Cytotoxicity Assay Kit Beyotime Cat#C2015 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062 Apoptosis and Necrosis Detection Kit with YO-PRO-1 and PI Beyotime Cat#C1075 LDH Cytotoxicity Assay Kit Beyotime Cat#C0017 Mouse IL-1β ELISA Kit YUBIBIO Cat#U96-1494E Mouse IL-18 ELISA Kit YUBIBIO Cat#U96-3279E Deposited data Bulk RNA sequencing data This paper [NCBI]: [PRJNA1286157] Experimental models: Cell lines Murine 4T1 breast cancer cell line ATCC Cat#CRL-2539 Murine B16F10 melanoma cell line ATCC Cat#CRL-6475 Experimental models: Organisms/strains Mouse: Female BALB/c Shanghai Model Organisms Center N/A Mouse: Female C57BL/6J Shanghai Model Organisms Center N/A Mouse: Female BALB/c-nu Shanghai Model Organisms Center N/A Software and algorithms GraphPad Prism (version 9.5.1) GraphPad Software https://www.graphpad.com/ Adobe Illustrator Adobe https://www.adobe.com/products/ illustrator/free-trial-download.html ZEN Zeiss https://www.zeiss.com/microscopy/ en/products/software/zeiss-zen.html FlowJo (version 10.8.1) BD Biosciences https://www.flowjo.com/ Living Image (version 4.1) Caliper Life Sciences https://living-image.software. informer.com/4.1/ e2 Cell Reports Medicine 6, 102312, September 16, 2025 Article ll OPEN ACCESS ..

    CCK-8 Assay:

    Article Title: Biomimetic nanoimmunotherapy boosts spatiotemporal PANoptosis and reshapes desmoplastic tumor microenvironment
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER (S)-Crizotinib Aladdin Cat#S288194 Tetrakis(4-carboxyphenyl)porphyrin (TCPP) TCI Cat#A5015 1-octadecene Sigma-Aldrich Cat#O806 Oleic acid Sigma-Aldrich Cat#364525 Polyethylene glycol (PEG) solution (50% (w/v)) Sigma-Aldrich Cat#P7306 Tetrahydrobiopterin (BH4, 11.212) Schircks Labs N/A Critical commercial assays Membrane and Cytosol Protein Extraction Kit Beyotime Cat#P0033 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033 Alexa Fluor 488–conjugated avidin Thermo Fisher Cat#S11223 Cell Counting Kit-8 Beyotime Cat#C0038 Calcein/PI Cell Viability/Cytotoxicity Assay Kit Beyotime Cat#C2015 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062 Apoptosis and Necrosis Detection Kit with YO-PRO-1 and PI Beyotime Cat#C1075 LDH Cytotoxicity Assay Kit Beyotime Cat#C0017 Mouse IL-1β ELISA Kit YUBIBIO Cat#U96-1494E Mouse IL-18 ELISA Kit YUBIBIO Cat#U96-3279E Deposited data Bulk RNA sequencing data This paper [NCBI]: [PRJNA1286157] Experimental models: Cell lines Murine 4T1 breast cancer cell line ATCC Cat#CRL-2539 Murine B16F10 melanoma cell line ATCC Cat#CRL-6475 Experimental models: Organisms/strains Mouse: Female BALB/c Shanghai Model Organisms Center N/A Mouse: Female C57BL/6J Shanghai Model Organisms Center N/A Mouse: Female BALB/c-nu Shanghai Model Organisms Center N/A Software and algorithms GraphPad Prism (version 9.5.1) GraphPad Software https://www.graphpad.com/ Adobe Illustrator Adobe https://www.adobe.com/products/ illustrator/free-trial-download.html ZEN Zeiss https://www.zeiss.com/microscopy/ en/products/software/zeiss-zen.html FlowJo (version 10.8.1) BD Biosciences https://www.flowjo.com/ Living Image (version 4.1) Caliper Life Sciences https://living-image.software. informer.com/4.1/ e2 Cell Reports Medicine 6, 102312, September 16, 2025 Article ll OPEN ACCESS ..

    LDH Cytotoxicity Assay:

    Article Title: Biomimetic nanoimmunotherapy boosts spatiotemporal PANoptosis and reshapes desmoplastic tumor microenvironment
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER (S)-Crizotinib Aladdin Cat#S288194 Tetrakis(4-carboxyphenyl)porphyrin (TCPP) TCI Cat#A5015 1-octadecene Sigma-Aldrich Cat#O806 Oleic acid Sigma-Aldrich Cat#364525 Polyethylene glycol (PEG) solution (50% (w/v)) Sigma-Aldrich Cat#P7306 Tetrahydrobiopterin (BH4, 11.212) Schircks Labs N/A Critical commercial assays Membrane and Cytosol Protein Extraction Kit Beyotime Cat#P0033 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033 Alexa Fluor 488–conjugated avidin Thermo Fisher Cat#S11223 Cell Counting Kit-8 Beyotime Cat#C0038 Calcein/PI Cell Viability/Cytotoxicity Assay Kit Beyotime Cat#C2015 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062 Apoptosis and Necrosis Detection Kit with YO-PRO-1 and PI Beyotime Cat#C1075 LDH Cytotoxicity Assay Kit Beyotime Cat#C0017 Mouse IL-1β ELISA Kit YUBIBIO Cat#U96-1494E Mouse IL-18 ELISA Kit YUBIBIO Cat#U96-3279E Deposited data Bulk RNA sequencing data This paper [NCBI]: [PRJNA1286157] Experimental models: Cell lines Murine 4T1 breast cancer cell line ATCC Cat#CRL-2539 Murine B16F10 melanoma cell line ATCC Cat#CRL-6475 Experimental models: Organisms/strains Mouse: Female BALB/c Shanghai Model Organisms Center N/A Mouse: Female C57BL/6J Shanghai Model Organisms Center N/A Mouse: Female BALB/c-nu Shanghai Model Organisms Center N/A Software and algorithms GraphPad Prism (version 9.5.1) GraphPad Software https://www.graphpad.com/ Adobe Illustrator Adobe https://www.adobe.com/products/ illustrator/free-trial-download.html ZEN Zeiss https://www.zeiss.com/microscopy/ en/products/software/zeiss-zen.html FlowJo (version 10.8.1) BD Biosciences https://www.flowjo.com/ Living Image (version 4.1) Caliper Life Sciences https://living-image.software. informer.com/4.1/ e2 Cell Reports Medicine 6, 102312, September 16, 2025 Article ll OPEN ACCESS ..

    Article Title: Targeting ROS-metabolism dual pathways to trigger PANoptosis by cobalt-vanadium oxides biomimetic nanocakes for tumor immunotherapy.
    Article Snippet: To overcome the resistance of tumor cells to death through a single pathway, PANoptosis, a novel synergistic mode of cell death, can simultaneously activates pyroptosis, apoptosis, and necroptosis.. Most current strategies for inducing PANoptosis focused on amplifying reactive oxygen species (ROS) through exogenous stimuli, while the regulatory role of tumor cell metabolic characteristic in this process has long been overlooked.. We propose a ROS-Metabolism dual axis synergistic induction of PANoptosis, simultaneously enhancing antitumor immune responses, and construct a novel bionic nanoplatform named CVOVM.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Biomimetic nanoimmunotherapy boosts spatiotemporal PANoptosis and reshapes desmoplastic tumor microenvironment
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER (S)-Crizotinib Aladdin Cat#S288194 Tetrakis(4-carboxyphenyl)porphyrin (TCPP) TCI Cat#A5015 1-octadecene Sigma-Aldrich Cat#O806 Oleic acid Sigma-Aldrich Cat#364525 Polyethylene glycol (PEG) solution (50% (w/v)) Sigma-Aldrich Cat#P7306 Tetrahydrobiopterin (BH4, 11.212) Schircks Labs N/A Critical commercial assays Membrane and Cytosol Protein Extraction Kit Beyotime Cat#P0033 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033 Alexa Fluor 488–conjugated avidin Thermo Fisher Cat#S11223 Cell Counting Kit-8 Beyotime Cat#C0038 Calcein/PI Cell Viability/Cytotoxicity Assay Kit Beyotime Cat#C2015 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062 Apoptosis and Necrosis Detection Kit with YO-PRO-1 and PI Beyotime Cat#C1075 LDH Cytotoxicity Assay Kit Beyotime Cat#C0017 Mouse IL-1β ELISA Kit YUBIBIO Cat#U96-1494E Mouse IL-18 ELISA Kit YUBIBIO Cat#U96-3279E Deposited data Bulk RNA sequencing data This paper [NCBI]: [PRJNA1286157] Experimental models: Cell lines Murine 4T1 breast cancer cell line ATCC Cat#CRL-2539 Murine B16F10 melanoma cell line ATCC Cat#CRL-6475 Experimental models: Organisms/strains Mouse: Female BALB/c Shanghai Model Organisms Center N/A Mouse: Female C57BL/6J Shanghai Model Organisms Center N/A Mouse: Female BALB/c-nu Shanghai Model Organisms Center N/A Software and algorithms GraphPad Prism (version 9.5.1) GraphPad Software https://www.graphpad.com/ Adobe Illustrator Adobe https://www.adobe.com/products/ illustrator/free-trial-download.html ZEN Zeiss https://www.zeiss.com/microscopy/ en/products/software/zeiss-zen.html FlowJo (version 10.8.1) BD Biosciences https://www.flowjo.com/ Living Image (version 4.1) Caliper Life Sciences https://living-image.software. informer.com/4.1/ e2 Cell Reports Medicine 6, 102312, September 16, 2025 Article ll OPEN ACCESS ..

    RNA Sequencing:

    Article Title: Biomimetic nanoimmunotherapy boosts spatiotemporal PANoptosis and reshapes desmoplastic tumor microenvironment
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER (S)-Crizotinib Aladdin Cat#S288194 Tetrakis(4-carboxyphenyl)porphyrin (TCPP) TCI Cat#A5015 1-octadecene Sigma-Aldrich Cat#O806 Oleic acid Sigma-Aldrich Cat#364525 Polyethylene glycol (PEG) solution (50% (w/v)) Sigma-Aldrich Cat#P7306 Tetrahydrobiopterin (BH4, 11.212) Schircks Labs N/A Critical commercial assays Membrane and Cytosol Protein Extraction Kit Beyotime Cat#P0033 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033 Alexa Fluor 488–conjugated avidin Thermo Fisher Cat#S11223 Cell Counting Kit-8 Beyotime Cat#C0038 Calcein/PI Cell Viability/Cytotoxicity Assay Kit Beyotime Cat#C2015 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062 Apoptosis and Necrosis Detection Kit with YO-PRO-1 and PI Beyotime Cat#C1075 LDH Cytotoxicity Assay Kit Beyotime Cat#C0017 Mouse IL-1β ELISA Kit YUBIBIO Cat#U96-1494E Mouse IL-18 ELISA Kit YUBIBIO Cat#U96-3279E Deposited data Bulk RNA sequencing data This paper [NCBI]: [PRJNA1286157] Experimental models: Cell lines Murine 4T1 breast cancer cell line ATCC Cat#CRL-2539 Murine B16F10 melanoma cell line ATCC Cat#CRL-6475 Experimental models: Organisms/strains Mouse: Female BALB/c Shanghai Model Organisms Center N/A Mouse: Female C57BL/6J Shanghai Model Organisms Center N/A Mouse: Female BALB/c-nu Shanghai Model Organisms Center N/A Software and algorithms GraphPad Prism (version 9.5.1) GraphPad Software https://www.graphpad.com/ Adobe Illustrator Adobe https://www.adobe.com/products/ illustrator/free-trial-download.html ZEN Zeiss https://www.zeiss.com/microscopy/ en/products/software/zeiss-zen.html FlowJo (version 10.8.1) BD Biosciences https://www.flowjo.com/ Living Image (version 4.1) Caliper Life Sciences https://living-image.software. informer.com/4.1/ e2 Cell Reports Medicine 6, 102312, September 16, 2025 Article ll OPEN ACCESS ..

    Software:

    Article Title: Biomimetic nanoimmunotherapy boosts spatiotemporal PANoptosis and reshapes desmoplastic tumor microenvironment
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER (S)-Crizotinib Aladdin Cat#S288194 Tetrakis(4-carboxyphenyl)porphyrin (TCPP) TCI Cat#A5015 1-octadecene Sigma-Aldrich Cat#O806 Oleic acid Sigma-Aldrich Cat#364525 Polyethylene glycol (PEG) solution (50% (w/v)) Sigma-Aldrich Cat#P7306 Tetrahydrobiopterin (BH4, 11.212) Schircks Labs N/A Critical commercial assays Membrane and Cytosol Protein Extraction Kit Beyotime Cat#P0033 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033 Alexa Fluor 488–conjugated avidin Thermo Fisher Cat#S11223 Cell Counting Kit-8 Beyotime Cat#C0038 Calcein/PI Cell Viability/Cytotoxicity Assay Kit Beyotime Cat#C2015 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062 Apoptosis and Necrosis Detection Kit with YO-PRO-1 and PI Beyotime Cat#C1075 LDH Cytotoxicity Assay Kit Beyotime Cat#C0017 Mouse IL-1β ELISA Kit YUBIBIO Cat#U96-1494E Mouse IL-18 ELISA Kit YUBIBIO Cat#U96-3279E Deposited data Bulk RNA sequencing data This paper [NCBI]: [PRJNA1286157] Experimental models: Cell lines Murine 4T1 breast cancer cell line ATCC Cat#CRL-2539 Murine B16F10 melanoma cell line ATCC Cat#CRL-6475 Experimental models: Organisms/strains Mouse: Female BALB/c Shanghai Model Organisms Center N/A Mouse: Female C57BL/6J Shanghai Model Organisms Center N/A Mouse: Female BALB/c-nu Shanghai Model Organisms Center N/A Software and algorithms GraphPad Prism (version 9.5.1) GraphPad Software https://www.graphpad.com/ Adobe Illustrator Adobe https://www.adobe.com/products/ illustrator/free-trial-download.html ZEN Zeiss https://www.zeiss.com/microscopy/ en/products/software/zeiss-zen.html FlowJo (version 10.8.1) BD Biosciences https://www.flowjo.com/ Living Image (version 4.1) Caliper Life Sciences https://living-image.software. informer.com/4.1/ e2 Cell Reports Medicine 6, 102312, September 16, 2025 Article ll OPEN ACCESS ..

    Double Staining:

    Article Title: Transferrin receptor serves a role in promoting PANoptosis in thyroid cancer
    Article Snippet: Transfections were performed using Lipo8000 TM transfection reagent (cat. no. C0533-7.5 ml; Beyotime Institute of Biotechnology), with BCPAP cells transfected with TFRC overexpression plasmid or its NC (blank PGMLV-CMV-MCS-EF1-ZsGreen1-T2A-Puro plasmid) and K1 cells transfected with TFRC shRNA or its NC (oligo control). .. The PI/Calcein-AM Double Staining Kit (cat. no. CA1630; Beijing Solarbio Science & Technology Co., Ltd.) and Apoptosis and Necrosis Detection Kit with YO-PRO-1 and PI Kit (cat. no. C1075S; Beyotime Institute of Biotechnology) were used to assess PANoptosis in BCPAP and K1 cells. ..

    Concentration Assay:

    Article Title: Emestrin‐Type Epidithiodiketopiperazines Inhibited Gasdermin D‐Mediated Pyroptosis via Caspase‐3/7 Activation
    Article Snippet: The starved cells were then primed with 1 μg/mL LPS (Sigma; L4391) and 3 mM ATP (Sigma; A2383), and supplemented with either vehicle or the indicated compounds for 3 h. Subsequently, the cells were treated with 10 μM nigericin (Selleck; S6653) for 1 h. Both the cells and culture medium were collected for further analysis. .. For HCS, THP‐1 cells were differentiated with PMA and seeded into 96‐well plates at a density of 6 × 10 4 cells/well for 48 h. Prior to treatment, the cells were starved in RPMI 1640 medium for 24 h. Starved cells were then primed with 1 μg/mL LPS and 3 mM ATP, each supplemented with one of 536 different compounds in separate wells at a final concentration of 1 μM for 3 h. Following this, cells were treated with 10 μM Nigericin and 1 μM YO‐PRO‐1 (Beyotime; C2022) for 1 h. The cells were washed with PBS and fixed with paraformaldehyde (PFA) for 10 min. .. Fixed cells were then imaged and analyzed using the Opera Phenix HCS system (Revvity).

    Labeling:

    Article Title: Targeting HIF-1α rescues microglial efferocytosis via the SLC7A11-TAM pathway to ameliorate Sepsis-associated encephalopathy.
    Article Snippet: Sepsis-associated encephalopathy (SAE) is a common and debilitating complication of sepsis, yet its cellular mechanisms and targeted therapies remain unclear.. Microglia preserve neuroinflammatory homeostasis and neural circuit integrity through efferocytosis, but how this process is altered in SAE and regulated by immunometabolism is poorly defined.. Here, we investigated the molecular basis of microglial efferocytosis impairment using LPS-stimulated BV2 cells and a cecal ligation and puncture (CLP) murine SAE model. We integrated RNA sequencing, HIF-1α and SLC7A11 gainand loss-of-function approaches and in vitro functional assays.

    ATP Assay:

    Article Title: Targeting ROS-metabolism dual pathways to trigger PANoptosis by cobalt-vanadium oxides biomimetic nanocakes for tumor immunotherapy.
    Article Snippet: To overcome the resistance of tumor cells to death through a single pathway, PANoptosis, a novel synergistic mode of cell death, can simultaneously activates pyroptosis, apoptosis, and necroptosis.. Most current strategies for inducing PANoptosis focused on amplifying reactive oxygen species (ROS) through exogenous stimuli, while the regulatory role of tumor cell metabolic characteristic in this process has long been overlooked.. We propose a ROS-Metabolism dual axis synergistic induction of PANoptosis, simultaneously enhancing antitumor immune responses, and construct a novel bionic nanoplatform named CVOVM.

    other:

    Article Title: A biomimetic arsenic-based nanozyme enhances dual epigenetic regulation to improve the efficacy of immunotherapy for acute myeloid leukaemia.
    Article Snippet: Aberrant epigenetic modifications in acute myeloid leukaemia (AML) limit immunotherapeutic efficacy.. Arsenic ions are capable of undergoing valence transformation in organisms, exhibit favourable enzyme-mimetic activity, and thereby play a crucial role in enhancing dual epigenetic regulation.. Therefore, to achieve potent epigenetic regulation, in this study, a biomimetic arsenic-based nanozyme (As/ZIF-8@M) that exhibits leukaemia cell recognition and phagocytosis capabilities, as well as bone marrow homing effects, was engineered.



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    PPF suppresses NLRP3-mediated pyroptosis in BV2 cells following OGD/R. (A) SEM observation of BV2 cell morphology (magnification, ×20,000; scale bar, 2 μ <t>m).</t> <t>(B)</t> <t>Yo-Pro-1</t> and Hoechst 33342 staining for assessing the pyroptosis levels in BV-2 cells. (C) Quantitative analysis of Yo-Pro-1 and Hoechst 33342 staining revealed that PPF deceased pyroptosis levels in BV-2 cells. (D) Immunofluorescence staining of NLRP3 in BV2 cells (magnification, ×40; scale bar, 50 μ m). (E) Immunofluorescence staining of ASC in BV2 cells (magnification, ×40; scale bar, 50 μ m). (F) Quantitative analysis of immunofluorescence staining revealed that NLRP3 and ASC fluorescence intensity increased in OGD/R-treated BV2 cells, while PPF decreased the fluorescence intensity of both proteins. (G) Caspase-1 activity was detected in BV2 cells following OGD/R using Caspase-1 Activity Assay kit. (H) Western blotting of NLRP3, ASC, cleaved-caspase-1, pro-caspase-1, GSDMD-N, GSDMD, IL-18, IL-1β, and GAPDH (internal reference). (I) Western blot analysis revealed elevated NLRP3, ASC, IL-1β and IL-18, cleaved-caspase-1/pro-caspase-1 levels (J), and GSDMD-N/GSDMD levels (K) in OGD/R-treated BV2 cells, PPF treatment reduced these proteins levels. (L) ELISA showed that PPF decreased IL-1β and IL-18 levels. ** P<0.01. PPF, Propofol; OGD/R, Oxygen-glucose deprivation/reoxygenation; ASC, apoptosis-associated speck-like protein containing a CARD; GSDMD, gasdermin D.
    Beyo3dtm Yo Pro 1, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PPF suppresses NLRP3-mediated pyroptosis in BV2 cells following OGD/R. (A) SEM observation of BV2 cell morphology (magnification, ×20,000; scale bar, 2 μ <t>m).</t> <t>(B)</t> <t>Yo-Pro-1</t> and Hoechst 33342 staining for assessing the pyroptosis levels in BV-2 cells. (C) Quantitative analysis of Yo-Pro-1 and Hoechst 33342 staining revealed that PPF deceased pyroptosis levels in BV-2 cells. (D) Immunofluorescence staining of NLRP3 in BV2 cells (magnification, ×40; scale bar, 50 μ m). (E) Immunofluorescence staining of ASC in BV2 cells (magnification, ×40; scale bar, 50 μ m). (F) Quantitative analysis of immunofluorescence staining revealed that NLRP3 and ASC fluorescence intensity increased in OGD/R-treated BV2 cells, while PPF decreased the fluorescence intensity of both proteins. (G) Caspase-1 activity was detected in BV2 cells following OGD/R using Caspase-1 Activity Assay kit. (H) Western blotting of NLRP3, ASC, cleaved-caspase-1, pro-caspase-1, GSDMD-N, GSDMD, IL-18, IL-1β, and GAPDH (internal reference). (I) Western blot analysis revealed elevated NLRP3, ASC, IL-1β and IL-18, cleaved-caspase-1/pro-caspase-1 levels (J), and GSDMD-N/GSDMD levels (K) in OGD/R-treated BV2 cells, PPF treatment reduced these proteins levels. (L) ELISA showed that PPF decreased IL-1β and IL-18 levels. ** P<0.01. PPF, Propofol; OGD/R, Oxygen-glucose deprivation/reoxygenation; ASC, apoptosis-associated speck-like protein containing a CARD; GSDMD, gasdermin D.
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    PPF suppresses NLRP3-mediated pyroptosis in BV2 cells following OGD/R. (A) SEM observation of BV2 cell morphology (magnification, ×20,000; scale bar, 2 μ <t>m).</t> <t>(B)</t> <t>Yo-Pro-1</t> and Hoechst 33342 staining for assessing the pyroptosis levels in BV-2 cells. (C) Quantitative analysis of Yo-Pro-1 and Hoechst 33342 staining revealed that PPF deceased pyroptosis levels in BV-2 cells. (D) Immunofluorescence staining of NLRP3 in BV2 cells (magnification, ×40; scale bar, 50 μ m). (E) Immunofluorescence staining of ASC in BV2 cells (magnification, ×40; scale bar, 50 μ m). (F) Quantitative analysis of immunofluorescence staining revealed that NLRP3 and ASC fluorescence intensity increased in OGD/R-treated BV2 cells, while PPF decreased the fluorescence intensity of both proteins. (G) Caspase-1 activity was detected in BV2 cells following OGD/R using Caspase-1 Activity Assay kit. (H) Western blotting of NLRP3, ASC, cleaved-caspase-1, pro-caspase-1, GSDMD-N, GSDMD, IL-18, IL-1β, and GAPDH (internal reference). (I) Western blot analysis revealed elevated NLRP3, ASC, IL-1β and IL-18, cleaved-caspase-1/pro-caspase-1 levels (J), and GSDMD-N/GSDMD levels (K) in OGD/R-treated BV2 cells, PPF treatment reduced these proteins levels. (L) ELISA showed that PPF decreased IL-1β and IL-18 levels. ** P<0.01. PPF, Propofol; OGD/R, Oxygen-glucose deprivation/reoxygenation; ASC, apoptosis-associated speck-like protein containing a CARD; GSDMD, gasdermin D.
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    PPF suppresses NLRP3-mediated pyroptosis in BV2 cells following OGD/R. (A) SEM observation of BV2 cell morphology (magnification, ×20,000; scale bar, 2 μ <t>m).</t> <t>(B)</t> <t>Yo-Pro-1</t> and Hoechst 33342 staining for assessing the pyroptosis levels in BV-2 cells. (C) Quantitative analysis of Yo-Pro-1 and Hoechst 33342 staining revealed that PPF deceased pyroptosis levels in BV-2 cells. (D) Immunofluorescence staining of NLRP3 in BV2 cells (magnification, ×40; scale bar, 50 μ m). (E) Immunofluorescence staining of ASC in BV2 cells (magnification, ×40; scale bar, 50 μ m). (F) Quantitative analysis of immunofluorescence staining revealed that NLRP3 and ASC fluorescence intensity increased in OGD/R-treated BV2 cells, while PPF decreased the fluorescence intensity of both proteins. (G) Caspase-1 activity was detected in BV2 cells following OGD/R using Caspase-1 Activity Assay kit. (H) Western blotting of NLRP3, ASC, cleaved-caspase-1, pro-caspase-1, GSDMD-N, GSDMD, IL-18, IL-1β, and GAPDH (internal reference). (I) Western blot analysis revealed elevated NLRP3, ASC, IL-1β and IL-18, cleaved-caspase-1/pro-caspase-1 levels (J), and GSDMD-N/GSDMD levels (K) in OGD/R-treated BV2 cells, PPF treatment reduced these proteins levels. (L) ELISA showed that PPF decreased IL-1β and IL-18 levels. ** P<0.01. PPF, Propofol; OGD/R, Oxygen-glucose deprivation/reoxygenation; ASC, apoptosis-associated speck-like protein containing a CARD; GSDMD, gasdermin D.
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    Image Search Results


    PPF suppresses NLRP3-mediated pyroptosis in BV2 cells following OGD/R. (A) SEM observation of BV2 cell morphology (magnification, ×20,000; scale bar, 2 μ m). (B) Yo-Pro-1 and Hoechst 33342 staining for assessing the pyroptosis levels in BV-2 cells. (C) Quantitative analysis of Yo-Pro-1 and Hoechst 33342 staining revealed that PPF deceased pyroptosis levels in BV-2 cells. (D) Immunofluorescence staining of NLRP3 in BV2 cells (magnification, ×40; scale bar, 50 μ m). (E) Immunofluorescence staining of ASC in BV2 cells (magnification, ×40; scale bar, 50 μ m). (F) Quantitative analysis of immunofluorescence staining revealed that NLRP3 and ASC fluorescence intensity increased in OGD/R-treated BV2 cells, while PPF decreased the fluorescence intensity of both proteins. (G) Caspase-1 activity was detected in BV2 cells following OGD/R using Caspase-1 Activity Assay kit. (H) Western blotting of NLRP3, ASC, cleaved-caspase-1, pro-caspase-1, GSDMD-N, GSDMD, IL-18, IL-1β, and GAPDH (internal reference). (I) Western blot analysis revealed elevated NLRP3, ASC, IL-1β and IL-18, cleaved-caspase-1/pro-caspase-1 levels (J), and GSDMD-N/GSDMD levels (K) in OGD/R-treated BV2 cells, PPF treatment reduced these proteins levels. (L) ELISA showed that PPF decreased IL-1β and IL-18 levels. ** P<0.01. PPF, Propofol; OGD/R, Oxygen-glucose deprivation/reoxygenation; ASC, apoptosis-associated speck-like protein containing a CARD; GSDMD, gasdermin D.

    Journal: International Journal of Molecular Medicine

    Article Title: Propofol upregulates MFG-E8 in BV2 cells to inhibit pyroptosis mediated by the NF-κB/NLRP3 pathway, thereby ameliorating ischemic-reperfusion neuronal injury

    doi: 10.3892/ijmm.2026.5786

    Figure Lengend Snippet: PPF suppresses NLRP3-mediated pyroptosis in BV2 cells following OGD/R. (A) SEM observation of BV2 cell morphology (magnification, ×20,000; scale bar, 2 μ m). (B) Yo-Pro-1 and Hoechst 33342 staining for assessing the pyroptosis levels in BV-2 cells. (C) Quantitative analysis of Yo-Pro-1 and Hoechst 33342 staining revealed that PPF deceased pyroptosis levels in BV-2 cells. (D) Immunofluorescence staining of NLRP3 in BV2 cells (magnification, ×40; scale bar, 50 μ m). (E) Immunofluorescence staining of ASC in BV2 cells (magnification, ×40; scale bar, 50 μ m). (F) Quantitative analysis of immunofluorescence staining revealed that NLRP3 and ASC fluorescence intensity increased in OGD/R-treated BV2 cells, while PPF decreased the fluorescence intensity of both proteins. (G) Caspase-1 activity was detected in BV2 cells following OGD/R using Caspase-1 Activity Assay kit. (H) Western blotting of NLRP3, ASC, cleaved-caspase-1, pro-caspase-1, GSDMD-N, GSDMD, IL-18, IL-1β, and GAPDH (internal reference). (I) Western blot analysis revealed elevated NLRP3, ASC, IL-1β and IL-18, cleaved-caspase-1/pro-caspase-1 levels (J), and GSDMD-N/GSDMD levels (K) in OGD/R-treated BV2 cells, PPF treatment reduced these proteins levels. (L) ELISA showed that PPF decreased IL-1β and IL-18 levels. ** P<0.01. PPF, Propofol; OGD/R, Oxygen-glucose deprivation/reoxygenation; ASC, apoptosis-associated speck-like protein containing a CARD; GSDMD, gasdermin D.

    Article Snippet: Hoechst 33342 (cat. no. C1025) and Yo-Pro-1 staining solution (500 μ l; cat. no. C1356S; both Beyotime Institute of Biotechnology) were added at 37°C for 30 min.

    Techniques: Staining, Immunofluorescence, Fluorescence, Activity Assay, Western Blot, Enzyme-linked Immunosorbent Assay

    PPF suppresses pyroptosis caused by NF-κB/NLRP3 signaling by upregulating MFG-E8. (A) Western blotting protein bands for MFG-E8, p-NF-κB, NF-κB, and GAPDH (internal reference) in BV2 cells. (B) Western blotting revealed that PPF increased MFG-E8 and decreased p-NF-κB/NF-κB levels (C) in BV2 cells. (D) Western blotting for MFG-E8 and GAPDH in BV2 cells. (E) Following transfection of si-MFG-E8 into BV2 cells, MFG-E8 protein levels were significantly decreased. (F) Dual luciferase reporter gene assay confirmed that NF-κB is the target of MFG-E8. (G) Western blotting protein bands for p-NF-κB, NF-κB, and GAPDH (internal reference) in BV2 cells. (H) si-MFG-E8 attenuated the effects of PPF, resulting in elevated p-NF-κB/NF-κB levels. (I) Yo-Pro-1 and Hoechst 33342 staining for assessing the pyroptosis levels in BV-2 cells (magnification, ×40; scale bar, 50 μ m). (J) Yo-Pro-1 and Hoechst 33342 staining showed that PPF decreased Yo-Pro-1 positivity, while silencing MFG-E8 increased Yo-Pro-1 positivity. ELISA showed that PPF decreased TNF-α (K) and IL-1β (L) levels, silencing MFG-E8 reversed this effect. (M and N) ELISA showed that PPF raised IL-10 levels (M) and decreased IL-6 levels (N), silencing MFG-E8 reversed this effect. (O) Western blotting protein bands for NLRP3, ASC, cleaved-caspase-1, pro-caspase-1, GSDMD-N, GSDMD, IL-1β, IL-18, and GAPDH (internal reference) in BV2 cells. (P-R) Western blot analysis indicated that PPF decreased NLRP3, ASC, IL-1β, and IL-18 levels (P), GSDMD-N/GSDMD levels (Q), cleaved-caspase-1/pro-caspase-1 levels (R), silencing MFG-E8 increased these protein levels. ** P<0.01. PPF, propofol; MFG-E8, milk fat globule-EGF factor 8; OGD/R, Oxygen-glucose deprivation/reoxygenation; ASC, apoptosis-associated speck-like protein containing a CARD; GSDM, gasdermin; OE, overexpression; p-, phosphorylated; LPS, lipopolysaccharide; NC, negative control; ns, not significant; si, small interfering; WT, wild-type; MUT, mutant.

    Journal: International Journal of Molecular Medicine

    Article Title: Propofol upregulates MFG-E8 in BV2 cells to inhibit pyroptosis mediated by the NF-κB/NLRP3 pathway, thereby ameliorating ischemic-reperfusion neuronal injury

    doi: 10.3892/ijmm.2026.5786

    Figure Lengend Snippet: PPF suppresses pyroptosis caused by NF-κB/NLRP3 signaling by upregulating MFG-E8. (A) Western blotting protein bands for MFG-E8, p-NF-κB, NF-κB, and GAPDH (internal reference) in BV2 cells. (B) Western blotting revealed that PPF increased MFG-E8 and decreased p-NF-κB/NF-κB levels (C) in BV2 cells. (D) Western blotting for MFG-E8 and GAPDH in BV2 cells. (E) Following transfection of si-MFG-E8 into BV2 cells, MFG-E8 protein levels were significantly decreased. (F) Dual luciferase reporter gene assay confirmed that NF-κB is the target of MFG-E8. (G) Western blotting protein bands for p-NF-κB, NF-κB, and GAPDH (internal reference) in BV2 cells. (H) si-MFG-E8 attenuated the effects of PPF, resulting in elevated p-NF-κB/NF-κB levels. (I) Yo-Pro-1 and Hoechst 33342 staining for assessing the pyroptosis levels in BV-2 cells (magnification, ×40; scale bar, 50 μ m). (J) Yo-Pro-1 and Hoechst 33342 staining showed that PPF decreased Yo-Pro-1 positivity, while silencing MFG-E8 increased Yo-Pro-1 positivity. ELISA showed that PPF decreased TNF-α (K) and IL-1β (L) levels, silencing MFG-E8 reversed this effect. (M and N) ELISA showed that PPF raised IL-10 levels (M) and decreased IL-6 levels (N), silencing MFG-E8 reversed this effect. (O) Western blotting protein bands for NLRP3, ASC, cleaved-caspase-1, pro-caspase-1, GSDMD-N, GSDMD, IL-1β, IL-18, and GAPDH (internal reference) in BV2 cells. (P-R) Western blot analysis indicated that PPF decreased NLRP3, ASC, IL-1β, and IL-18 levels (P), GSDMD-N/GSDMD levels (Q), cleaved-caspase-1/pro-caspase-1 levels (R), silencing MFG-E8 increased these protein levels. ** P<0.01. PPF, propofol; MFG-E8, milk fat globule-EGF factor 8; OGD/R, Oxygen-glucose deprivation/reoxygenation; ASC, apoptosis-associated speck-like protein containing a CARD; GSDM, gasdermin; OE, overexpression; p-, phosphorylated; LPS, lipopolysaccharide; NC, negative control; ns, not significant; si, small interfering; WT, wild-type; MUT, mutant.

    Article Snippet: Hoechst 33342 (cat. no. C1025) and Yo-Pro-1 staining solution (500 μ l; cat. no. C1356S; both Beyotime Institute of Biotechnology) were added at 37°C for 30 min.

    Techniques: Western Blot, Transfection, Luciferase, Reporter Gene Assay, Staining, Enzyme-linked Immunosorbent Assay, Over Expression, Negative Control, Mutagenesis