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expression vectors pnic yfp  (Addgene inc)


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    Addgene inc expression vectors pnic yfp
    Expression Vectors Pnic Yfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/yfp+expression+vector/pm41619733-241-10-14?v=Addgene+inc
    Average 93 stars, based on 88 article reviews
    expression vectors pnic yfp - by Bioz Stars, 2026-07
    93/100 stars

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    a , LDH release assay in NLRP3-KO BlaER1 cells reconstituted with the indicated FLAG-tagged NLRP3 constructs or control plasmid. Expression was induced with doxycycline (Dox) for 18 h. Cells were primed with 200 ng/ml LPS for 4h followed by activation with 6.5 µM nigericin, 30 µg/ml imiquimod or 0.025 mg/ml needle toxin for 2 h. Data are represented as mean ± SD, n = 3. b , Western blots of the whole cell lysates from THP-1 NLRP3-KO cells reconstituted with human or zebrafish NLRP3 or zebrafish NLRP3 containing human PYD. Cells were treated with 1 µg/ml LPS for 3 h followed by activation with 20 µM nigericin or 200 µM imiquimod for 1 h. FLAG-tagged NLRP3 constructs and cleaved GSDMD were visualized with corresponding antibodies. c, <t>d,</t> <t>ASC</t> speck formation in HEK293T cells stably expressing <t>YFP-ASC,</t> upon transfection with indicated NLRP3 constructs: human, zebrafish NLRP3 and zebrafish NLRP3 with B30.2-domain deletion (zebrafish NLRP3 ΔB30.2 ) ( c ); wild-type and oligomer-disrupting mutants of zebrafish NLRP3 ( d ). Cells were transfected with the indicated amount of DNA, and the number of ASC specks per image area was calculated 24 h post-transfection. Data are represented as mean ± SD, n = 3.
    Yfp, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/yfp+expression+vector/bio_rxiv__64898__2026__04__17__719140-198-14-7?v=OriGene
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    expression vectors pnic yfp  (Addgene inc)
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    Addgene inc expression vectors pnic yfp
    a , LDH release assay in NLRP3-KO BlaER1 cells reconstituted with the indicated FLAG-tagged NLRP3 constructs or control plasmid. Expression was induced with doxycycline (Dox) for 18 h. Cells were primed with 200 ng/ml LPS for 4h followed by activation with 6.5 µM nigericin, 30 µg/ml imiquimod or 0.025 mg/ml needle toxin for 2 h. Data are represented as mean ± SD, n = 3. b , Western blots of the whole cell lysates from THP-1 NLRP3-KO cells reconstituted with human or zebrafish NLRP3 or zebrafish NLRP3 containing human PYD. Cells were treated with 1 µg/ml LPS for 3 h followed by activation with 20 µM nigericin or 200 µM imiquimod for 1 h. FLAG-tagged NLRP3 constructs and cleaved GSDMD were visualized with corresponding antibodies. c, <t>d,</t> <t>ASC</t> speck formation in HEK293T cells stably expressing <t>YFP-ASC,</t> upon transfection with indicated NLRP3 constructs: human, zebrafish NLRP3 and zebrafish NLRP3 with B30.2-domain deletion (zebrafish NLRP3 ΔB30.2 ) ( c ); wild-type and oligomer-disrupting mutants of zebrafish NLRP3 ( d ). Cells were transfected with the indicated amount of DNA, and the number of ASC specks per image area was calculated 24 h post-transfection. Data are represented as mean ± SD, n = 3.
    Expression Vectors Pnic Yfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    expression vectors pnic cfp  (Addgene inc)
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    a , LDH release assay in NLRP3-KO BlaER1 cells reconstituted with the indicated FLAG-tagged NLRP3 constructs or control plasmid. Expression was induced with doxycycline (Dox) for 18 h. Cells were primed with 200 ng/ml LPS for 4h followed by activation with 6.5 µM nigericin, 30 µg/ml imiquimod or 0.025 mg/ml needle toxin for 2 h. Data are represented as mean ± SD, n = 3. b , Western blots of the whole cell lysates from THP-1 NLRP3-KO cells reconstituted with human or zebrafish NLRP3 or zebrafish NLRP3 containing human PYD. Cells were treated with 1 µg/ml LPS for 3 h followed by activation with 20 µM nigericin or 200 µM imiquimod for 1 h. FLAG-tagged NLRP3 constructs and cleaved GSDMD were visualized with corresponding antibodies. c, <t>d,</t> <t>ASC</t> speck formation in HEK293T cells stably expressing <t>YFP-ASC,</t> upon transfection with indicated NLRP3 constructs: human, zebrafish NLRP3 and zebrafish NLRP3 with B30.2-domain deletion (zebrafish NLRP3 ΔB30.2 ) ( c ); wild-type and oligomer-disrupting mutants of zebrafish NLRP3 ( d ). Cells were transfected with the indicated amount of DNA, and the number of ASC specks per image area was calculated 24 h post-transfection. Data are represented as mean ± SD, n = 3.
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    a , LDH release assay in NLRP3-KO BlaER1 cells reconstituted with the indicated FLAG-tagged NLRP3 constructs or control plasmid. Expression was induced with doxycycline (Dox) for 18 h. Cells were primed with 200 ng/ml LPS for 4h followed by activation with 6.5 µM nigericin, 30 µg/ml imiquimod or 0.025 mg/ml needle toxin for 2 h. Data are represented as mean ± SD, n = 3. b , Western blots of the whole cell lysates from THP-1 NLRP3-KO cells reconstituted with human or zebrafish NLRP3 or zebrafish NLRP3 containing human PYD. Cells were treated with 1 µg/ml LPS for 3 h followed by activation with 20 µM nigericin or 200 µM imiquimod for 1 h. FLAG-tagged NLRP3 constructs and cleaved GSDMD were visualized with corresponding antibodies. c, <t>d,</t> <t>ASC</t> speck formation in HEK293T cells stably expressing <t>YFP-ASC,</t> upon transfection with indicated NLRP3 constructs: human, zebrafish NLRP3 and zebrafish NLRP3 with B30.2-domain deletion (zebrafish NLRP3 ΔB30.2 ) ( c ); wild-type and oligomer-disrupting mutants of zebrafish NLRP3 ( d ). Cells were transfected with the indicated amount of DNA, and the number of ASC specks per image area was calculated 24 h post-transfection. Data are represented as mean ± SD, n = 3.
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    a , LDH release assay in NLRP3-KO BlaER1 cells reconstituted with the indicated FLAG-tagged NLRP3 constructs or control plasmid. Expression was induced with doxycycline (Dox) for 18 h. Cells were primed with 200 ng/ml LPS for 4h followed by activation with 6.5 µM nigericin, 30 µg/ml imiquimod or 0.025 mg/ml needle toxin for 2 h. Data are represented as mean ± SD, n = 3. b , Western blots of the whole cell lysates from THP-1 NLRP3-KO cells reconstituted with human or zebrafish NLRP3 or zebrafish NLRP3 containing human PYD. Cells were treated with 1 µg/ml LPS for 3 h followed by activation with 20 µM nigericin or 200 µM imiquimod for 1 h. FLAG-tagged NLRP3 constructs and cleaved GSDMD were visualized with corresponding antibodies. c, <t>d,</t> <t>ASC</t> speck formation in HEK293T cells stably expressing <t>YFP-ASC,</t> upon transfection with indicated NLRP3 constructs: human, zebrafish NLRP3 and zebrafish NLRP3 with B30.2-domain deletion (zebrafish NLRP3 ΔB30.2 ) ( c ); wild-type and oligomer-disrupting mutants of zebrafish NLRP3 ( d ). Cells were transfected with the indicated amount of DNA, and the number of ASC specks per image area was calculated 24 h post-transfection. Data are represented as mean ± SD, n = 3.
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    a , LDH release assay in NLRP3-KO BlaER1 cells reconstituted with the indicated FLAG-tagged NLRP3 constructs or control plasmid. Expression was induced with doxycycline (Dox) for 18 h. Cells were primed with 200 ng/ml LPS for 4h followed by activation with 6.5 µM nigericin, 30 µg/ml imiquimod or 0.025 mg/ml needle toxin for 2 h. Data are represented as mean ± SD, n = 3. b , Western blots of the whole cell lysates from THP-1 NLRP3-KO cells reconstituted with human or zebrafish NLRP3 or zebrafish NLRP3 containing human PYD. Cells were treated with 1 µg/ml LPS for 3 h followed by activation with 20 µM nigericin or 200 µM imiquimod for 1 h. FLAG-tagged NLRP3 constructs and cleaved GSDMD were visualized with corresponding antibodies. c, <t>d,</t> <t>ASC</t> speck formation in HEK293T cells stably expressing <t>YFP-ASC,</t> upon transfection with indicated NLRP3 constructs: human, zebrafish NLRP3 and zebrafish NLRP3 with B30.2-domain deletion (zebrafish NLRP3 ΔB30.2 ) ( c ); wild-type and oligomer-disrupting mutants of zebrafish NLRP3 ( d ). Cells were transfected with the indicated amount of DNA, and the number of ASC specks per image area was calculated 24 h post-transfection. Data are represented as mean ± SD, n = 3.
    Pcdna5/Frt/To Expression Vector Containing Yfp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
    pcdna5/frt/to expression vector containing yfp - by Bioz Stars, 2026-07
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    pcmv6 ac yfp vector  (OriGene)
    90
    OriGene pcmv6 ac yfp vector
    a , LDH release assay in NLRP3-KO BlaER1 cells reconstituted with the indicated FLAG-tagged NLRP3 constructs or control plasmid. Expression was induced with doxycycline (Dox) for 18 h. Cells were primed with 200 ng/ml LPS for 4h followed by activation with 6.5 µM nigericin, 30 µg/ml imiquimod or 0.025 mg/ml needle toxin for 2 h. Data are represented as mean ± SD, n = 3. b , Western blots of the whole cell lysates from THP-1 NLRP3-KO cells reconstituted with human or zebrafish NLRP3 or zebrafish NLRP3 containing human PYD. Cells were treated with 1 µg/ml LPS for 3 h followed by activation with 20 µM nigericin or 200 µM imiquimod for 1 h. FLAG-tagged NLRP3 constructs and cleaved GSDMD were visualized with corresponding antibodies. c, <t>d,</t> <t>ASC</t> speck formation in HEK293T cells stably expressing <t>YFP-ASC,</t> upon transfection with indicated NLRP3 constructs: human, zebrafish NLRP3 and zebrafish NLRP3 with B30.2-domain deletion (zebrafish NLRP3 ΔB30.2 ) ( c ); wild-type and oligomer-disrupting mutants of zebrafish NLRP3 ( d ). Cells were transfected with the indicated amount of DNA, and the number of ASC specks per image area was calculated 24 h post-transfection. Data are represented as mean ± SD, n = 3.
    Pcmv6 Ac Yfp Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/yfp+expression+vector/pmc10617771-183-32-35?v=OriGene
    Average 90 stars, based on 1 article reviews
    pcmv6 ac yfp vector - by Bioz Stars, 2026-07
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    a , LDH release assay in NLRP3-KO BlaER1 cells reconstituted with the indicated FLAG-tagged NLRP3 constructs or control plasmid. Expression was induced with doxycycline (Dox) for 18 h. Cells were primed with 200 ng/ml LPS for 4h followed by activation with 6.5 µM nigericin, 30 µg/ml imiquimod or 0.025 mg/ml needle toxin for 2 h. Data are represented as mean ± SD, n = 3. b , Western blots of the whole cell lysates from THP-1 NLRP3-KO cells reconstituted with human or zebrafish NLRP3 or zebrafish NLRP3 containing human PYD. Cells were treated with 1 µg/ml LPS for 3 h followed by activation with 20 µM nigericin or 200 µM imiquimod for 1 h. FLAG-tagged NLRP3 constructs and cleaved GSDMD were visualized with corresponding antibodies. c, d, ASC speck formation in HEK293T cells stably expressing YFP-ASC, upon transfection with indicated NLRP3 constructs: human, zebrafish NLRP3 and zebrafish NLRP3 with B30.2-domain deletion (zebrafish NLRP3 ΔB30.2 ) ( c ); wild-type and oligomer-disrupting mutants of zebrafish NLRP3 ( d ). Cells were transfected with the indicated amount of DNA, and the number of ASC specks per image area was calculated 24 h post-transfection. Data are represented as mean ± SD, n = 3.

    Journal: bioRxiv

    Article Title: Structure of zebrafish NLRP3 reveals a novel mode of inflammasome activation

    doi: 10.64898/2026.04.17.719140

    Figure Lengend Snippet: a , LDH release assay in NLRP3-KO BlaER1 cells reconstituted with the indicated FLAG-tagged NLRP3 constructs or control plasmid. Expression was induced with doxycycline (Dox) for 18 h. Cells were primed with 200 ng/ml LPS for 4h followed by activation with 6.5 µM nigericin, 30 µg/ml imiquimod or 0.025 mg/ml needle toxin for 2 h. Data are represented as mean ± SD, n = 3. b , Western blots of the whole cell lysates from THP-1 NLRP3-KO cells reconstituted with human or zebrafish NLRP3 or zebrafish NLRP3 containing human PYD. Cells were treated with 1 µg/ml LPS for 3 h followed by activation with 20 µM nigericin or 200 µM imiquimod for 1 h. FLAG-tagged NLRP3 constructs and cleaved GSDMD were visualized with corresponding antibodies. c, d, ASC speck formation in HEK293T cells stably expressing YFP-ASC, upon transfection with indicated NLRP3 constructs: human, zebrafish NLRP3 and zebrafish NLRP3 with B30.2-domain deletion (zebrafish NLRP3 ΔB30.2 ) ( c ); wild-type and oligomer-disrupting mutants of zebrafish NLRP3 ( d ). Cells were transfected with the indicated amount of DNA, and the number of ASC specks per image area was calculated 24 h post-transfection. Data are represented as mean ± SD, n = 3.

    Article Snippet: Human full-length ASC was cloned into pLenti-EF1a-C-tGFP (Origene, PS100072) and tGFP was exchanged with YFP.

    Techniques: Lactate Dehydrogenase Assay, Construct, Control, Plasmid Preparation, Expressing, Activation Assay, Western Blot, Stable Transfection, Transfection

    a , Western blot analysis of NLRP3 expression in reconstituted cell lines in following doxycycline (Dox) induction. Bands corresponding to FLAG-tagged NLRP3 constructs are indicated with arrows. b, c , Western blot analysis of the whole cell lysates from HEK293T cells reconstituted with YFP-ASC and transfected with the indicated NLRP3 constructs, demonstrating relative expression levels of NLRP3 constructs used for ( b ) and ( c ). FLAG-tagged NLRP3 constructs and β-actin were visualized with corresponding antibodies. Cells were transfected with 100 ng DNA per well in a 96-well plate, and the lysates were collected 24 h post-transfection.

    Journal: bioRxiv

    Article Title: Structure of zebrafish NLRP3 reveals a novel mode of inflammasome activation

    doi: 10.64898/2026.04.17.719140

    Figure Lengend Snippet: a , Western blot analysis of NLRP3 expression in reconstituted cell lines in following doxycycline (Dox) induction. Bands corresponding to FLAG-tagged NLRP3 constructs are indicated with arrows. b, c , Western blot analysis of the whole cell lysates from HEK293T cells reconstituted with YFP-ASC and transfected with the indicated NLRP3 constructs, demonstrating relative expression levels of NLRP3 constructs used for ( b ) and ( c ). FLAG-tagged NLRP3 constructs and β-actin were visualized with corresponding antibodies. Cells were transfected with 100 ng DNA per well in a 96-well plate, and the lysates were collected 24 h post-transfection.

    Article Snippet: Human full-length ASC was cloned into pLenti-EF1a-C-tGFP (Origene, PS100072) and tGFP was exchanged with YFP.

    Techniques: Western Blot, Expressing, Construct, Transfection