Review



matchmaker gold yeast  (TaKaRa)


Bioz Verified Symbol TaKaRa is a verified supplier
Bioz Manufacturer Symbol TaKaRa manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    TaKaRa matchmaker gold yeast
    Matchmaker Gold Yeast, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 4252 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/yeast+matchmaker/Matchmaker+Gold+Yeast+Two-Hybrid+System/pm42127116-319-8-14
    Average 99 stars, based on 4252 article reviews
    matchmaker gold yeast - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    In Vivo:

    Article Title: Cytoplasmic relocation of Daxx induced by Ro52 and FLASH
    Article Snippet: .. The yeast Matchmaker Two-Hybrid System 3 (Clontech) was used to examine the in vivo interaction of these wild-type and truncated proteins. .. For the assays, the cDNA of Ro52 (full-length or truncated) was subcloned into pGBKT7 (a Gal4 DNA-binding domain vector for Gal4-BD fusion), and the cDNA of Daxx or FLASH (full-length or truncated) was subcloned into pGADT7 (a Gal4-activating domain vector for Gal4-AD fusion).

    Article Title: Interaction of NUB1 with the proteasome subunit S5a.
    Article Snippet: NUB1 interacts with a ubiquitin-like protein NEDD8 to target the NEDD8 monomer and neddylated proteins to the proteasome for degradation.. Therefore, NUB1 is thought to be a potent downregulator of NEDD8 conjugation system.. Since NUB1 possesses a UBL domain, which was previously shown to be an S5a-interacting motif in RAD23/HHR23, we initially hypothesized that NUB1 interacts with the S5a subunit of the proteasome through its UBL domain.

    Article Title: NUB1 Suppresses the Formation of Lewy Body-Like Inclusions by Proteasomal Degradation of Synphilin-1
    Article Snippet: .. 21 The yeast Matchmaker Two-Hybrid System 3 (Clontech) was used to examine the in vivo interaction of synphilin-1 with these mutants. .. To do so, the cDNA of synphilin-1 was subcloned into pGADT7 (a Gal4 DNA-activating domain vector for Gal4-AD fusion), and the cDNA of each mutant of NUB1 was subcloned into pGBKT7 (a Gal4 DNA-binding domain vector for Gal4-BD fusion).

    Control:

    Article Title: Inducible Yeast Two-Hybrid with Quantitative Measures
    Article Snippet: .. Yeast Matchmaker Two-Hybrid System was obtained from Clontech (now Takara Bio), including pGBKT7 bait vector, pGADT7 prey vector, and pGBKT7- Lamin (human lamin C amino acids 66-230), pGBKT7- p53 (mouse p53 amino acids 72-390), and pGADT7- Large-T (SV40 large-T antigen amino acids 84-708) as control plasmids. ..

    Mutagenesis:

    Article Title: Interaction of NUB1 with the proteasome subunit S5a.
    Article Snippet: NUB1 interacts with a ubiquitin-like protein NEDD8 to target the NEDD8 monomer and neddylated proteins to the proteasome for degradation.. Therefore, NUB1 is thought to be a potent downregulator of NEDD8 conjugation system.. Since NUB1 possesses a UBL domain, which was previously shown to be an S5a-interacting motif in RAD23/HHR23, we initially hypothesized that NUB1 interacts with the S5a subunit of the proteasome through its UBL domain.

    Protein-Protein interactions:

    Article Title: Compositions and methods for regulating SAS1R
    Article Snippet: .. Analysis of SLLP1-SAS1R Interactions by Yeast Matchmaker Two-Hybrid (Y2H) System 3: Protein—protein interactions between mouse SLLP1 and mouse SAS1R were studied in the advanced GAL4 based yeast two-hybrid system 3 (Clontech, CA). ..



    Similar Products

    matchmaker gold yeast  (TaKaRa)
    99
    TaKaRa matchmaker gold yeast
    Matchmaker Gold Yeast, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/yeast+matchmaker/Matchmaker+Gold+Yeast+Two-Hybrid+System/pm42127116-319-8-14
    Average 99 stars, based on 1 article reviews
    matchmaker gold yeast - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    two hybrid system  (TaKaRa)
    99
    TaKaRa two hybrid system
    Protein interactions between UvBhlh6 and other bHLH transcription factors. (a) <t>Yeast</t> <t>two‐hybrid</t> assay assessing interactions between UvBhlh6 and UvBhlh1, UvBhlh4 and UvBhlh7. Yeast cells (10 2 –10 6 cells/mL) co‐transformed with prey and bait vectors were spotted on SD/−Leu/−Trp and SD/−Ade/−His/−Leu/−Trp media. Positive control: PGADT7 (AD‐T) + pGBKT7‐53 (BD‐53); negative control: PGADT7 (AD‐T) + pGBKT7‐Lam (BD‐Lam). (b) Bimolecular fluorescence complementation assay detecting interactions between UvBhlh6 and UvBhlh1, UvBhlh4 or UvBhlh7. YFP signals and DAPI staining were visualised using an LSM710 confocal microscope (Zeiss). Scale bar, 10 μm.
    Two Hybrid System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/yeast+matchmaker/Matchmaker+Gold+Yeast+Two-Hybrid+System/pmc13136075-204-9-11
    Average 99 stars, based on 1 article reviews
    two hybrid system - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    one hybrid library screening system  (TaKaRa)
    98
    TaKaRa one hybrid library screening system
    Protein interactions between UvBhlh6 and other bHLH transcription factors. (a) <t>Yeast</t> <t>two‐hybrid</t> assay assessing interactions between UvBhlh6 and UvBhlh1, UvBhlh4 and UvBhlh7. Yeast cells (10 2 –10 6 cells/mL) co‐transformed with prey and bait vectors were spotted on SD/−Leu/−Trp and SD/−Ade/−His/−Leu/−Trp media. Positive control: PGADT7 (AD‐T) + pGBKT7‐53 (BD‐53); negative control: PGADT7 (AD‐T) + pGBKT7‐Lam (BD‐Lam). (b) Bimolecular fluorescence complementation assay detecting interactions between UvBhlh6 and UvBhlh1, UvBhlh4 or UvBhlh7. YFP signals and DAPI staining were visualised using an LSM710 confocal microscope (Zeiss). Scale bar, 10 μm.
    One Hybrid Library Screening System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/yeast+matchmaker/Matchmaker+Gold+Yeast+One-Hybrid+Library+Screening+System/10__1016_slash_j__indcrop__2026__123372-72-13-17
    Average 98 stars, based on 1 article reviews
    one hybrid library screening system - by Bioz Stars, 2026-09
    98/100 stars
    		  Buy from Supplier
    cd601 02 yeast strain ah109 takara  (TaKaRa)
    99
    TaKaRa cd601 02 yeast strain ah109 takara
    Protein interactions between UvBhlh6 and other bHLH transcription factors. (a) <t>Yeast</t> <t>two‐hybrid</t> assay assessing interactions between UvBhlh6 and UvBhlh1, UvBhlh4 and UvBhlh7. Yeast cells (10 2 –10 6 cells/mL) co‐transformed with prey and bait vectors were spotted on SD/−Leu/−Trp and SD/−Ade/−His/−Leu/−Trp media. Positive control: PGADT7 (AD‐T) + pGBKT7‐53 (BD‐53); negative control: PGADT7 (AD‐T) + pGBKT7‐Lam (BD‐Lam). (b) Bimolecular fluorescence complementation assay detecting interactions between UvBhlh6 and UvBhlh1, UvBhlh4 or UvBhlh7. YFP signals and DAPI staining were visualised using an LSM710 confocal microscope (Zeiss). Scale bar, 10 μm.
    Cd601 02 Yeast Strain Ah109 Takara, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/yeast+matchmaker/Matchmaker+Gold+Yeast+Two-Hybrid+System/pm42054208-566-71-75
    Average 99 stars, based on 1 article reviews
    cd601 02 yeast strain ah109 takara - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Protein interactions between UvBhlh6 and other bHLH transcription factors. (a) Yeast two‐hybrid assay assessing interactions between UvBhlh6 and UvBhlh1, UvBhlh4 and UvBhlh7. Yeast cells (10 2 –10 6 cells/mL) co‐transformed with prey and bait vectors were spotted on SD/−Leu/−Trp and SD/−Ade/−His/−Leu/−Trp media. Positive control: PGADT7 (AD‐T) + pGBKT7‐53 (BD‐53); negative control: PGADT7 (AD‐T) + pGBKT7‐Lam (BD‐Lam). (b) Bimolecular fluorescence complementation assay detecting interactions between UvBhlh6 and UvBhlh1, UvBhlh4 or UvBhlh7. YFP signals and DAPI staining were visualised using an LSM710 confocal microscope (Zeiss). Scale bar, 10 μm.

    Journal: Molecular Plant Pathology

    Article Title: The Basic Helix–Loop–Helix Transcription Factors UvBhlh1 and UvBhlh6 Coordinate Virulence in the Rice False Smut Fungus Ustilaginoidea virens

    doi: 10.1111/mpp.70267

    Figure Lengend Snippet: Protein interactions between UvBhlh6 and other bHLH transcription factors. (a) Yeast two‐hybrid assay assessing interactions between UvBhlh6 and UvBhlh1, UvBhlh4 and UvBhlh7. Yeast cells (10 2 –10 6 cells/mL) co‐transformed with prey and bait vectors were spotted on SD/−Leu/−Trp and SD/−Ade/−His/−Leu/−Trp media. Positive control: PGADT7 (AD‐T) + pGBKT7‐53 (BD‐53); negative control: PGADT7 (AD‐T) + pGBKT7‐Lam (BD‐Lam). (b) Bimolecular fluorescence complementation assay detecting interactions between UvBhlh6 and UvBhlh1, UvBhlh4 or UvBhlh7. YFP signals and DAPI staining were visualised using an LSM710 confocal microscope (Zeiss). Scale bar, 10 μm.

    Article Snippet: Protein–protein interactions were analysed using the Matchmaker Gold Yeast Two‐Hybrid System (Clontech).

    Techniques: Y2H Assay, Transformation Assay, Positive Control, Negative Control, Bimolecular Fluorescence Complementation Assay, Staining, Microscopy

    Subcellular localisation and protein interactions involving UvBhlh6. (a) Co‐localisation of DAPI and UvBhlh1‐eGFP, UvBhlh4‐eGFP, UvBhlh6‐eGFP and UvBhlh7‐eGFP in mycelia. Scale bar, 10 μm. (b) Yeast two‐hybrid assay assessing interactions between UvBhlh6 and its putative interacting partners identified by affinity capture‐mass spectrometry. Yeast cells were spotted on SD/−Leu/−Trp and SD/−Ade/−His/−Leu/−Trp media. Controls: Positive, pGADT7 (AD‐T) + pGBKT7‐53 (BD‐53); negative, pGADT7 (AD‐T) + pGBKT7‐Lam (BD‐Lam).

    Journal: Molecular Plant Pathology

    Article Title: The Basic Helix–Loop–Helix Transcription Factors UvBhlh1 and UvBhlh6 Coordinate Virulence in the Rice False Smut Fungus Ustilaginoidea virens

    doi: 10.1111/mpp.70267

    Figure Lengend Snippet: Subcellular localisation and protein interactions involving UvBhlh6. (a) Co‐localisation of DAPI and UvBhlh1‐eGFP, UvBhlh4‐eGFP, UvBhlh6‐eGFP and UvBhlh7‐eGFP in mycelia. Scale bar, 10 μm. (b) Yeast two‐hybrid assay assessing interactions between UvBhlh6 and its putative interacting partners identified by affinity capture‐mass spectrometry. Yeast cells were spotted on SD/−Leu/−Trp and SD/−Ade/−His/−Leu/−Trp media. Controls: Positive, pGADT7 (AD‐T) + pGBKT7‐53 (BD‐53); negative, pGADT7 (AD‐T) + pGBKT7‐Lam (BD‐Lam).

    Article Snippet: Protein–protein interactions were analysed using the Matchmaker Gold Yeast Two‐Hybrid System (Clontech).

    Techniques: Y2H Assay, Mass Spectrometry