y 27632 (Tocris)

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Tocris y 27632
Y 27632, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 2642 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/y+27632/Y-27632+dihydrochloride/pm42860416-483-55-56
Average 96 stars, based on 2642 article reviews

y 27632 - by Bioz Stars, 2026-10

96/100 stars

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Cell Culture:

Article Title: Charting the transition from in vitro gliogenesis to the in vivo maturation of human glial progenitor cells transplanted into the hypomyelinated mouse brain.
Article Snippet: Electroporation was performed using an Amaxa 4D-Nucleofector (Lonza) with the P3 primary cell kit (Lonza, V4XP-3024) according to manufacturer’s guidelines. .. Following nucleofection, cells were transferred to 10 cm cell culture dishes and cultured with mTeSR1 supplemented with 10 μM Y-27632 (Tocris, 1254) for 24h. .. Antibiotic selection was initiated 48–72h post-electroporation using 0.5 μg ml−1 puromycin (Thermo Fisher Scientific, A1113803).

Article Title: The gene-regulatory evolution of the human skeleton.
Article Snippet: .. In brief, iPS cells were counted and seeded at 9,000 cells per well in triplicate onto Matrigel-coated 24-well plates (10 μl ml−1 in DMEM, at least 1 h at 37 °C) and cultured for 24 h in mTeSR1 (STEMCELL Technologies, 85850) supplemented with Y-27632 (Tocris, 1254, 10 μM) and penicillin–streptomycin (25 IU ml−1). ..

other:

Article Title: Tissue‐Stimulator Platform for Electrically Stimulating Pancreatic β‐Cells for Long‐Term Functional Regulation
Article Snippet: Briefly, the fresh porcine pancreatic tissue was purchased from a local butcher, chopped into small pieces, and then washed with distilled water for 6 h. Subsequently, the sliced tissues were treated seven times with 1% Triton X-100 solution (T1020; Biosesang, Republic of Korea) for 12 h each.

Stem Cell:

Article Title: Molecular and Proteomic Profiling of Human Cerebral Organoid‑Derived Small Extracellular Vesicles
Article Snippet: .. Briefly, hiPSCs were plated on a low‐attachment U‐bottom 96 well plate with embryonic stem cell media (ESCM) (DMEM/F‐12 (Gibco, Thermo Fisher Scientific, 31330‐038), 20% KnockOut Serum Replacement (KOSR) (Gibco, Thermo Fisher Scientific, 10828028), 1× MEM Non‐Essential Amino Acids Solution (MEM‐NEAA) (Lonza, 11140050), 1× GlutaMAX Supplement (Gibco, Thermo Fisher Scientific, 35050061), 1% Penicillin‐Streptomycin (Gibco, Thermo Fisher Scientific, 15140122), 0.385 mM 2‐mercaptoethanol (Gibco, Thermo Fisher Scientific, 21985023), Y‐27632 (50 μM) (Tocris, 1254), 4 ng/mL Human FGF‐basic Recombinant Protein (only for the first 4 days) (Gibco, Thermo Fisher Scientific, Cat no. PHG0024) and incubated with medium change every other day for the generation of the embryoid bodies (EBs). .. On day 6, EBs were transferred into the low attachment 12 well plates with Neural Induction Media (NIM) (DMEM/F‐12) supplemented with 1× N‐2 MAX Media Supplement (R&D Systems, AR009), 1× GlutaMAX, 1× MEM‐NEAA, 1 μg/mL Heparin (Sigma‐Aldrich, H3149) for 4 days.

Knock-Out:

Recombinant:

Incubation:

Centrifugation:

Article Title: Retinal organoid–based evaluation of icariin-loaded extracellular vesicles for hypoxia-induced retinal injury via mitochondrial modulation
Article Snippet: Passaging was conducted every 4–5 days by treating the hESC colonies with Accutase (Stem Cell Technologies) at 37 °C for 5 min when 70%–80% confluency. .. Following the detachment of hESCs as single cells, they were resuspended in mTeSR1 supplemented with 20 μM Y-27632 (Tocris, Bristol, City of Bristol, United Kingdom), pelleted by centrifugation at 1,350 rpm for 10 min at room temperature, and subsequently passaged at a ratio 1:10 to 1:50. .. After passaging, the remaining cells are stored at −80 °C in a stock solution containing 90% KnockOut Serum Replacement (KOSR; Gibco, Waltham, MA, USA) and 10% dimethyl sulfoxide (DMSO; Duchefa Biochemie, Haarlem, North Holland, Netherlands).

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