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wide field inverted microscope  (Nikon)


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    Structured Review

    Nikon wide field inverted microscope
    Wide Field Inverted Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 14722 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wide+field+microscope/Inverted+Microscopes/pm41786829-121-38-41
    Average 99 stars, based on 14722 article reviews
    wide field inverted microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Imaging:

    Article Title: CoVET: Cryo-Electron Tomography guided by optical electrophysiology
    Article Snippet: .. Imaging was performed using an inverted wide-field microscope (Eclipse Ti2, Nikon) with an sCMOS camera (ORCA-Flash 4, Hamamatsu) and a 10× objective lens (numerical aperture: 0.45; CFI Plan Apo Lambda D 10X, Nikon). .. A high-power red LED (Solis 623C, Thorlabs) was used as the excitation source for BeRST1, with the irradiance set to less than 18 mW/mm 2 to avoid photodamage to neurons.

    Article Title: Correlative voltage imaging and cryo-electron tomography bridge neuronal activity and molecular structure
    Article Snippet: .. Imaging was performed using an inverted wide-field microscope (Eclipse Ti2, Nikon) with an sCMOS camera (ORCA-Flash 4, Hamamatsu) and a 10× objective lens (numerical aperture: 0.45; CFI Plan Apo Lambda D 10X, Nikon). .. A high-power red LED (Solis 623 C, Thorlabs) was used as the excitation source for BeRST1, with the irradiance set to less than 18 mW/mm2 to avoid photodamage to neurons.

    Article Title: Molecular tools and methods for transgene integration and their transposition dependent expression
    Article Snippet: The cells growing in glass-bottom 35 mm dish (Matek) were incubated in a microscope chamber at 37° C., under 5% CO2 in a humidified atmosphere. .. For time-lapse imaging, we used an inverted wide field microscope (Nikon Ti Eclipse) operated by Micromanager software equipped with a sCMOS Camera (Orca Flash4LT, Hamamatsu) and a 10× objective (CFI Plan APO LBDA, NA 0.45, Nikon). .. The cells growing in glass-bottom 35 mm dish (Matek) were incubated in a microscope chamber at 37° C., under 5% CO2 in a humidified atmosphere.

    Article Title: LED-based multicolor extended resolution transmission fluorescence microscopy
    Article Snippet: For biological sample imaging, the paraffin section of mouse kidney tissue was stained with iFluor 488 wheat germ agglutinin (WGA) (excitation: 491 nm; emission: 516 nm), iFluor 546 Vimentin (excitation: 541 nm; emission: 557 nm), and iFluor 647 α -smooth muscle actin (α-SMA) (excitation: 656 nm; emission: 670 nm), purchased from Wuhan Servicebio Technology Co., Ltd., China. .. The prototype imaging system used in this study is based on an inverted wide-field microscope (TE300, Nikon, Tokyo, Japan). ..

    Microscopy:

    Article Title: CoVET: Cryo-Electron Tomography guided by optical electrophysiology
    Article Snippet: .. Imaging was performed using an inverted wide-field microscope (Eclipse Ti2, Nikon) with an sCMOS camera (ORCA-Flash 4, Hamamatsu) and a 10× objective lens (numerical aperture: 0.45; CFI Plan Apo Lambda D 10X, Nikon). .. A high-power red LED (Solis 623C, Thorlabs) was used as the excitation source for BeRST1, with the irradiance set to less than 18 mW/mm 2 to avoid photodamage to neurons.

    Article Title: Supplementary Information for: Continuous monitoring biosensing mediated by single-molecule plasmon-enhanced fluorescence in complex matrices
    Article Snippet: .. Both scattering and fluorescence image sequences were measured by objective-type total internal reflection microscopy on an inverted wide-field microscope (Nikon Ti2). ..

    Article Title: Correlative voltage imaging and cryo-electron tomography bridge neuronal activity and molecular structure
    Article Snippet: .. Imaging was performed using an inverted wide-field microscope (Eclipse Ti2, Nikon) with an sCMOS camera (ORCA-Flash 4, Hamamatsu) and a 10× objective lens (numerical aperture: 0.45; CFI Plan Apo Lambda D 10X, Nikon). .. A high-power red LED (Solis 623 C, Thorlabs) was used as the excitation source for BeRST1, with the irradiance set to less than 18 mW/mm2 to avoid photodamage to neurons.

    Article Title: Molecular tools and methods for transgene integration and their transposition dependent expression
    Article Snippet: The cells growing in glass-bottom 35 mm dish (Matek) were incubated in a microscope chamber at 37° C., under 5% CO2 in a humidified atmosphere. .. For time-lapse imaging, we used an inverted wide field microscope (Nikon Ti Eclipse) operated by Micromanager software equipped with a sCMOS Camera (Orca Flash4LT, Hamamatsu) and a 10× objective (CFI Plan APO LBDA, NA 0.45, Nikon). .. The cells growing in glass-bottom 35 mm dish (Matek) were incubated in a microscope chamber at 37° C., under 5% CO2 in a humidified atmosphere.

    Article Title: Rapid pronucleus assembly using cytoplasmic RNAs in fertilized eggs of <i>Xenopus laevis</i>
    Article Snippet: The small hypercondensed sperm nucleus undergoes a dramatic transformation into a large round pronucleus with relaxed chromatin during the brief cleavage period in metazoan embryos, enabling the activation of chromatin functions necessary for subsequent development.. However, it remains unclear whether the egg cytoplasm-specific physicochemical properties play a role in pronucleus assembly.. Here, we evaluated the impact of abundant RNAs in eggs on pronucleus assembly in Xenopus laevis cell-free reconstitution system.

    Article Title: Supporting Information (SI) for Remodeling of perturbed chromatin can initiate de novo transcriptional and post-transcriptional silencing
    Article Snippet: .. Images were acquired on an inverted wide-field microscope (Nikon TE2000) equipped with a 100×/1.4 NA immersion objective and a Prime BSI Express sCMOS camera (Teledyne Photometrics) with 6.5×6.5 μm pixel size. .. A SPECTRA X Light Engine lamp (Lumencor) set to power 50, for 100 ms, was used to illuminate the samples.

    Article Title: LED-based multicolor extended resolution transmission fluorescence microscopy
    Article Snippet: For biological sample imaging, the paraffin section of mouse kidney tissue was stained with iFluor 488 wheat germ agglutinin (WGA) (excitation: 491 nm; emission: 516 nm), iFluor 546 Vimentin (excitation: 541 nm; emission: 557 nm), and iFluor 647 α -smooth muscle actin (α-SMA) (excitation: 656 nm; emission: 670 nm), purchased from Wuhan Servicebio Technology Co., Ltd., China. .. The prototype imaging system used in this study is based on an inverted wide-field microscope (TE300, Nikon, Tokyo, Japan). ..

    Article Title: Enhancing power density and cycle life of NMC811 battery cathodes via combined dense calendering and laser patterning
    Article Snippet: .. This consisted of a custom-built inverted wide-field microscope fitted with an air objective (20×/NA0.4, TU Plan EPI ELWD, Nikon) with wide-field illumination (740 nm, Thorlabs fiber-coupled LED, M740F2). ..

    Fluorescence:

    Article Title: Supplementary Information for: Continuous monitoring biosensing mediated by single-molecule plasmon-enhanced fluorescence in complex matrices
    Article Snippet: .. Both scattering and fluorescence image sequences were measured by objective-type total internal reflection microscopy on an inverted wide-field microscope (Nikon Ti2). ..

    Software:

    Article Title: Molecular tools and methods for transgene integration and their transposition dependent expression
    Article Snippet: The cells growing in glass-bottom 35 mm dish (Matek) were incubated in a microscope chamber at 37° C., under 5% CO2 in a humidified atmosphere. .. For time-lapse imaging, we used an inverted wide field microscope (Nikon Ti Eclipse) operated by Micromanager software equipped with a sCMOS Camera (Orca Flash4LT, Hamamatsu) and a 10× objective (CFI Plan APO LBDA, NA 0.45, Nikon). .. The cells growing in glass-bottom 35 mm dish (Matek) were incubated in a microscope chamber at 37° C., under 5% CO2 in a humidified atmosphere.

    Glass:

    Article Title: Rapid pronucleus assembly using cytoplasmic RNAs in fertilized eggs of <i>Xenopus laevis</i>
    Article Snippet: The small hypercondensed sperm nucleus undergoes a dramatic transformation into a large round pronucleus with relaxed chromatin during the brief cleavage period in metazoan embryos, enabling the activation of chromatin functions necessary for subsequent development.. However, it remains unclear whether the egg cytoplasm-specific physicochemical properties play a role in pronucleus assembly.. Here, we evaluated the impact of abundant RNAs in eggs on pronucleus assembly in Xenopus laevis cell-free reconstitution system.



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    Image Search Results


    Wide-field epifluorescence microscope optical layout. Schematic showing illumination and detection beam paths, component positions, and optical layout. Component specifications detailed in .

    Journal: bioRxiv

    Article Title: Multimodal immobilization of second-instar Drosophila melanogaster larvae using PF-127 hydrogel and diethyl ether for calcium imaging

    doi: 10.64898/2026.03.19.713048

    Figure Lengend Snippet: Wide-field epifluorescence microscope optical layout. Schematic showing illumination and detection beam paths, component positions, and optical layout. Component specifications detailed in .

    Article Snippet: All images were acquired using a custom-built wide-field epifluorescence microscope ( ) constructed on a Thorlabs 30 mm cage system with a cage cube as the central junction for excitation and emission paths.

    Techniques: Microscopy