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wfa  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc wfa
    B. Quantification <t>of</t> <t>GFAP</t> + cell counts for the total striatum. Data was normalized to area. C. Quantification of GFAP + intensity per cell. D. Quantification of GFAP + cell bodv area in the striatum. E. Quantification of average endpoints per GFAP + cell in the striatum. F. Sholl analysis of the branch profile (ramification) of GFAP + cells in the striatum. G. Percentage ofPNNs associated with a GFAP + cells. H. Quantification of WTA intensity per GFAP + cell. I. Quantification of distance of GFAP + cells to soma of PNN-ensheathed neurons. J. Linear regression analysis of GFAP intensity versus <t>WFA</t> + intensity per cell. R 2 values of corresponding linear regressions label on graph. Data represented as mean ± SEM, data was tested for normality prior to statistical analysis. Two-tailed t-tests or Welch’s t-test used when appropriate. Sholl analysis: Two-Way ANOVA with Tukey. Regression analysis: t-test comparison of slopes, p-values: * <0.05, ** <0.01. N = 4-5 mice/group, 3-5 sections/mouse, 13-41 cells/mouse.
    Wfa, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wfa/bio_rxiv__64898__2026__03__25__714174-7-64-58
    Average 86 stars, based on 1 article reviews
    wfa - by Bioz Stars, 2026-10
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    1) Product Images from "Glial cell and perineuronal net interactions in the dorsal striatum of aged mice"

    Article Title: Glial cell and perineuronal net interactions in the dorsal striatum of aged mice

    Journal: bioRxiv

    doi: 10.64898/2026.03.25.714174

    B. Quantification of GFAP + cell counts for the total striatum. Data was normalized to area. C. Quantification of GFAP + intensity per cell. D. Quantification of GFAP + cell bodv area in the striatum. E. Quantification of average endpoints per GFAP + cell in the striatum. F. Sholl analysis of the branch profile (ramification) of GFAP + cells in the striatum. G. Percentage ofPNNs associated with a GFAP + cells. H. Quantification of WTA intensity per GFAP + cell. I. Quantification of distance of GFAP + cells to soma of PNN-ensheathed neurons. J. Linear regression analysis of GFAP intensity versus WFA + intensity per cell. R 2 values of corresponding linear regressions label on graph. Data represented as mean ± SEM, data was tested for normality prior to statistical analysis. Two-tailed t-tests or Welch’s t-test used when appropriate. Sholl analysis: Two-Way ANOVA with Tukey. Regression analysis: t-test comparison of slopes, p-values: * <0.05, ** <0.01. N = 4-5 mice/group, 3-5 sections/mouse, 13-41 cells/mouse.
    Figure Legend Snippet: B. Quantification of GFAP + cell counts for the total striatum. Data was normalized to area. C. Quantification of GFAP + intensity per cell. D. Quantification of GFAP + cell bodv area in the striatum. E. Quantification of average endpoints per GFAP + cell in the striatum. F. Sholl analysis of the branch profile (ramification) of GFAP + cells in the striatum. G. Percentage ofPNNs associated with a GFAP + cells. H. Quantification of WTA intensity per GFAP + cell. I. Quantification of distance of GFAP + cells to soma of PNN-ensheathed neurons. J. Linear regression analysis of GFAP intensity versus WFA + intensity per cell. R 2 values of corresponding linear regressions label on graph. Data represented as mean ± SEM, data was tested for normality prior to statistical analysis. Two-tailed t-tests or Welch’s t-test used when appropriate. Sholl analysis: Two-Way ANOVA with Tukey. Regression analysis: t-test comparison of slopes, p-values: * <0.05, ** <0.01. N = 4-5 mice/group, 3-5 sections/mouse, 13-41 cells/mouse.

    Techniques Used: Two Tailed Test, Comparison

    Related Articles

    Staining:

    Article Title: Glial cell and perineuronal net interactions in the dorsal striatum of aged mice
    Article Snippet: .. Coronal 30-μm hemisections containing the dorsal striatum were processed in two independent staining/imaging cohorts: ( ) microglia were labeled with Iba1 (rabbit; FUJIFILM Wako, #P3088) in combination with the lysosomal/phagocytic marker CD68 (rat; Bio-Rad, #MCA1957) and perineuronal nets (PNNs) were visualized using fluorescein-conjugated Wisteria floribunda agglutinin (WFA; Vector Laboratories, #FL-1351); ( ) astrocytes were labeled with GFAP (rabbit; Cell Signaling Technology, #12389) together with WFA to visualize PNNs. .. Secondary antibodies included goat anti-rat Alexa Fluor 594 (Invitrogen, #A48264), goat anti-rabbit Alexa Fluor 635 (Invitrogen, #A31577), and goat anti-mouse Alexa Fluor 594 (Invitrogen, #A21125), as appropriate for each experiment.

    Labeling:

    Article Title: Glial cell and perineuronal net interactions in the dorsal striatum of aged mice
    Article Snippet: .. Coronal 30-μm hemisections containing the dorsal striatum were processed in two independent staining/imaging cohorts: ( ) microglia were labeled with Iba1 (rabbit; FUJIFILM Wako, #P3088) in combination with the lysosomal/phagocytic marker CD68 (rat; Bio-Rad, #MCA1957) and perineuronal nets (PNNs) were visualized using fluorescein-conjugated Wisteria floribunda agglutinin (WFA; Vector Laboratories, #FL-1351); ( ) astrocytes were labeled with GFAP (rabbit; Cell Signaling Technology, #12389) together with WFA to visualize PNNs. .. Secondary antibodies included goat anti-rat Alexa Fluor 594 (Invitrogen, #A48264), goat anti-rabbit Alexa Fluor 635 (Invitrogen, #A31577), and goat anti-mouse Alexa Fluor 594 (Invitrogen, #A21125), as appropriate for each experiment.

    Marker:

    Article Title: Glial cell and perineuronal net interactions in the dorsal striatum of aged mice
    Article Snippet: .. Coronal 30-μm hemisections containing the dorsal striatum were processed in two independent staining/imaging cohorts: ( ) microglia were labeled with Iba1 (rabbit; FUJIFILM Wako, #P3088) in combination with the lysosomal/phagocytic marker CD68 (rat; Bio-Rad, #MCA1957) and perineuronal nets (PNNs) were visualized using fluorescein-conjugated Wisteria floribunda agglutinin (WFA; Vector Laboratories, #FL-1351); ( ) astrocytes were labeled with GFAP (rabbit; Cell Signaling Technology, #12389) together with WFA to visualize PNNs. .. Secondary antibodies included goat anti-rat Alexa Fluor 594 (Invitrogen, #A48264), goat anti-rabbit Alexa Fluor 635 (Invitrogen, #A31577), and goat anti-mouse Alexa Fluor 594 (Invitrogen, #A21125), as appropriate for each experiment.



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    wfa  (Cell Signaling Technology Inc)
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    B. Quantification <t>of</t> <t>GFAP</t> + cell counts for the total striatum. Data was normalized to area. C. Quantification of GFAP + intensity per cell. D. Quantification of GFAP + cell bodv area in the striatum. E. Quantification of average endpoints per GFAP + cell in the striatum. F. Sholl analysis of the branch profile (ramification) of GFAP + cells in the striatum. G. Percentage ofPNNs associated with a GFAP + cells. H. Quantification of WTA intensity per GFAP + cell. I. Quantification of distance of GFAP + cells to soma of PNN-ensheathed neurons. J. Linear regression analysis of GFAP intensity versus <t>WFA</t> + intensity per cell. R 2 values of corresponding linear regressions label on graph. Data represented as mean ± SEM, data was tested for normality prior to statistical analysis. Two-tailed t-tests or Welch’s t-test used when appropriate. Sholl analysis: Two-Way ANOVA with Tukey. Regression analysis: t-test comparison of slopes, p-values: * <0.05, ** <0.01. N = 4-5 mice/group, 3-5 sections/mouse, 13-41 cells/mouse.
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    Image Search Results


    B. Quantification of GFAP + cell counts for the total striatum. Data was normalized to area. C. Quantification of GFAP + intensity per cell. D. Quantification of GFAP + cell bodv area in the striatum. E. Quantification of average endpoints per GFAP + cell in the striatum. F. Sholl analysis of the branch profile (ramification) of GFAP + cells in the striatum. G. Percentage ofPNNs associated with a GFAP + cells. H. Quantification of WTA intensity per GFAP + cell. I. Quantification of distance of GFAP + cells to soma of PNN-ensheathed neurons. J. Linear regression analysis of GFAP intensity versus WFA + intensity per cell. R 2 values of corresponding linear regressions label on graph. Data represented as mean ± SEM, data was tested for normality prior to statistical analysis. Two-tailed t-tests or Welch’s t-test used when appropriate. Sholl analysis: Two-Way ANOVA with Tukey. Regression analysis: t-test comparison of slopes, p-values: * <0.05, ** <0.01. N = 4-5 mice/group, 3-5 sections/mouse, 13-41 cells/mouse.

    Journal: bioRxiv

    Article Title: Glial cell and perineuronal net interactions in the dorsal striatum of aged mice

    doi: 10.64898/2026.03.25.714174

    Figure Lengend Snippet: B. Quantification of GFAP + cell counts for the total striatum. Data was normalized to area. C. Quantification of GFAP + intensity per cell. D. Quantification of GFAP + cell bodv area in the striatum. E. Quantification of average endpoints per GFAP + cell in the striatum. F. Sholl analysis of the branch profile (ramification) of GFAP + cells in the striatum. G. Percentage ofPNNs associated with a GFAP + cells. H. Quantification of WTA intensity per GFAP + cell. I. Quantification of distance of GFAP + cells to soma of PNN-ensheathed neurons. J. Linear regression analysis of GFAP intensity versus WFA + intensity per cell. R 2 values of corresponding linear regressions label on graph. Data represented as mean ± SEM, data was tested for normality prior to statistical analysis. Two-tailed t-tests or Welch’s t-test used when appropriate. Sholl analysis: Two-Way ANOVA with Tukey. Regression analysis: t-test comparison of slopes, p-values: * <0.05, ** <0.01. N = 4-5 mice/group, 3-5 sections/mouse, 13-41 cells/mouse.

    Article Snippet: Coronal 30-μm hemisections containing the dorsal striatum were processed in two independent staining/imaging cohorts: ( ) microglia were labeled with Iba1 (rabbit; FUJIFILM Wako, #P3088) in combination with the lysosomal/phagocytic marker CD68 (rat; Bio-Rad, #MCA1957) and perineuronal nets (PNNs) were visualized using fluorescein-conjugated Wisteria floribunda agglutinin (WFA; Vector Laboratories, #FL-1351); ( ) astrocytes were labeled with GFAP (rabbit; Cell Signaling Technology, #12389) together with WFA to visualize PNNs.

    Techniques: Two Tailed Test, Comparison