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vsvg
Vsvg, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 634 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vsvg/VSV%2EG+(Plasmid+%2314888)/pm41922773-579-11-12
Average 96 stars, based on 634 article reviews
Vsvg, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 634 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vsvg/VSV%2EG+(Plasmid+%2314888)/pm41922773-579-11-12
Average 96 stars, based on 634 article reviews
vsvg - by Bioz Stars,
2026-09
96/100 stars
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Plasmid Preparation:Article Title: Pre-existing cell states predict resistance to multiple treatments. Article Snippet: .. Per plate, we combined 500 μL of OPTI-MEM (Gibco, 31985062) with 80 μL of PEI (Polysciences, 23966-1) in a tube while combining 500 μL OPTI-MEM with 10 μg barcode plasmid, 5 μg Article Title: Adaptive response to electrical pulse stimulation is impaired in FSHD myotubes by DUX4 gene network activation Article Snippet: The PCR product was cloned into the modified vector using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs) and confirmed by Sanger sequencing (Azenta). .. Lentiviral particles were produced in 293T cells via co-transfection of pCW57.1-LEUTX or empty pCW57.1(Addgene plasmid #41393), PAX2 (Addgene #12260), and Article Title: Induced Proprioceptor and Low‐Threshold Mechanoreceptor Neurons Derived from Human Pluripotent Stem Cells Exhibit Distinct Functional Mechanosensory Properties Article Snippet: For lentiviral production, HEK293T cells were passaged with Accutase (#00‐4555‐56, ThermoFisher) and were seeded at a density of 5000 000 cells/T75 flask. .. Lentiviral particles were produced 24 h after seeding (90–100% confluence) by co‐transfecting 12 μg of the plasmid encoding for the expression vector of interest, or 12 μg of the reverse tetracycline transactivator vector FUW‐M2rtTA (#20342, Addgene), with the lentiviral packaging plasmids: 6 μg pMDL (#12251, Addgene), 3 μg Article Title: Induced Proprioceptor and Low-Threshold Mechanoreceptor Neurons Derived from Human Pluripotent Stem Cells Exhibit Distinct Functional Mechanosensory Properties. Article Snippet: For lentiviral production, HEK293T cells were passaged with Accutase (#00-4555-56, ThermoFisher) and were seeded at a density of 5000 000 cells/T75 flask. .. Lentiviral particles were produced 24 h after seeding (90–100% confluence) by co-transfecting 12 μg of the plasmid encoding for the expression vector of interest, or 12 μg of the reverse tetracycline transactivator vector FUW-M2rtTA (#20342, Addgene), with the lentiviral packaging plasmids: 6 μg pMDL (#12251, Addgene), 3 μg Article Title: Adaptive response to electrical pulse stimulation is impaired in FSHD myotubes by DUX4 gene network activation. Article Snippet: The PCR product was cloned into the modified vector using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs) and confirmed by Sanger sequencing (Azenta). .. Lentiviral particles were produced in 293T cells via co-transfection of pCW57.1-LEUTX or empty pCW57.1(Addgene plasmid #41393), PAX2 (Addgene #12260), and Produced:Article Title: Adaptive response to electrical pulse stimulation is impaired in FSHD myotubes by DUX4 gene network activation Article Snippet: The PCR product was cloned into the modified vector using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs) and confirmed by Sanger sequencing (Azenta). .. Lentiviral particles were produced in 293T cells via co-transfection of pCW57.1-LEUTX or empty pCW57.1(Addgene plasmid #41393), PAX2 (Addgene #12260), and Article Title: Coordinated DNA methyltransferase 3A and methyltransferase-like 7A activity reprograms the tumor microenvironment through discoidin domain receptor 1 signaling. Article Snippet: .. Cell transduction and experimental groups Lentiviral particles were produced in HEK293T cells using Lipofectamine 2000 (#11668030; Thermo Fisher, Waltham, MA, USA). pHAGE-puro (#118692; Addgene, Watertown, MA, USA) was co-transfected with pSPAX2 (#12260; Addgene, Watertown, MA, USA) and pMD2.G (#12259; Addgene, Watertown, MA, USA) for overexpression. pSuper-retro-puro was packaged with gag/pol (#14887; Addgene, Watertown, MA, USA) and Article Title: Coordinated DNA methyltransferase 3A and methyltransferase-like 7A activity reprograms the tumor microenvironment through discoidin domain receptor 1 signaling Article Snippet: .. Lentiviral particles were produced in HEK293T cells using Lipofectamine 2000 (#11668030; Thermo Fisher, Waltham, MA, USA). pHAGE-puro (#118692; Addgene, Watertown, MA, USA) was co-transfected with pSPAX2 (#12260; Addgene, Watertown, MA, USA) and pMD2.G (#12259; Addgene, Watertown, MA, USA) for overexpression. pSuper-retro-puro was packaged with gag/pol (#14887; Addgene, Watertown, MA, USA) and Article Title: Induced Proprioceptor and Low‐Threshold Mechanoreceptor Neurons Derived from Human Pluripotent Stem Cells Exhibit Distinct Functional Mechanosensory Properties Article Snippet: For lentiviral production, HEK293T cells were passaged with Accutase (#00‐4555‐56, ThermoFisher) and were seeded at a density of 5000 000 cells/T75 flask. .. Lentiviral particles were produced 24 h after seeding (90–100% confluence) by co‐transfecting 12 μg of the plasmid encoding for the expression vector of interest, or 12 μg of the reverse tetracycline transactivator vector FUW‐M2rtTA (#20342, Addgene), with the lentiviral packaging plasmids: 6 μg pMDL (#12251, Addgene), 3 μg Article Title: Induced Proprioceptor and Low-Threshold Mechanoreceptor Neurons Derived from Human Pluripotent Stem Cells Exhibit Distinct Functional Mechanosensory Properties. Article Snippet: For lentiviral production, HEK293T cells were passaged with Accutase (#00-4555-56, ThermoFisher) and were seeded at a density of 5000 000 cells/T75 flask. .. Lentiviral particles were produced 24 h after seeding (90–100% confluence) by co-transfecting 12 μg of the plasmid encoding for the expression vector of interest, or 12 μg of the reverse tetracycline transactivator vector FUW-M2rtTA (#20342, Addgene), with the lentiviral packaging plasmids: 6 μg pMDL (#12251, Addgene), 3 μg Article Title: Adaptive response to electrical pulse stimulation is impaired in FSHD myotubes by DUX4 gene network activation. Article Snippet: The PCR product was cloned into the modified vector using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs) and confirmed by Sanger sequencing (Azenta). .. Lentiviral particles were produced in 293T cells via co-transfection of pCW57.1-LEUTX or empty pCW57.1(Addgene plasmid #41393), PAX2 (Addgene #12260), and Transduction:Article Title: Coordinated DNA methyltransferase 3A and methyltransferase-like 7A activity reprograms the tumor microenvironment through discoidin domain receptor 1 signaling. Article Snippet: .. Cell transduction and experimental groups Lentiviral particles were produced in HEK293T cells using Lipofectamine 2000 (#11668030; Thermo Fisher, Waltham, MA, USA). pHAGE-puro (#118692; Addgene, Watertown, MA, USA) was co-transfected with pSPAX2 (#12260; Addgene, Watertown, MA, USA) and pMD2.G (#12259; Addgene, Watertown, MA, USA) for overexpression. pSuper-retro-puro was packaged with gag/pol (#14887; Addgene, Watertown, MA, USA) and Over Expression:Article Title: Coordinated DNA methyltransferase 3A and methyltransferase-like 7A activity reprograms the tumor microenvironment through discoidin domain receptor 1 signaling. Article Snippet: .. Cell transduction and experimental groups Lentiviral particles were produced in HEK293T cells using Lipofectamine 2000 (#11668030; Thermo Fisher, Waltham, MA, USA). pHAGE-puro (#118692; Addgene, Watertown, MA, USA) was co-transfected with pSPAX2 (#12260; Addgene, Watertown, MA, USA) and pMD2.G (#12259; Addgene, Watertown, MA, USA) for overexpression. pSuper-retro-puro was packaged with gag/pol (#14887; Addgene, Watertown, MA, USA) and Article Title: Coordinated DNA methyltransferase 3A and methyltransferase-like 7A activity reprograms the tumor microenvironment through discoidin domain receptor 1 signaling Article Snippet: .. Lentiviral particles were produced in HEK293T cells using Lipofectamine 2000 (#11668030; Thermo Fisher, Waltham, MA, USA). pHAGE-puro (#118692; Addgene, Watertown, MA, USA) was co-transfected with pSPAX2 (#12260; Addgene, Watertown, MA, USA) and pMD2.G (#12259; Addgene, Watertown, MA, USA) for overexpression. pSuper-retro-puro was packaged with gag/pol (#14887; Addgene, Watertown, MA, USA) and Knockdown:Article Title: Coordinated DNA methyltransferase 3A and methyltransferase-like 7A activity reprograms the tumor microenvironment through discoidin domain receptor 1 signaling. Article Snippet: .. Cell transduction and experimental groups Lentiviral particles were produced in HEK293T cells using Lipofectamine 2000 (#11668030; Thermo Fisher, Waltham, MA, USA). pHAGE-puro (#118692; Addgene, Watertown, MA, USA) was co-transfected with pSPAX2 (#12260; Addgene, Watertown, MA, USA) and pMD2.G (#12259; Addgene, Watertown, MA, USA) for overexpression. pSuper-retro-puro was packaged with gag/pol (#14887; Addgene, Watertown, MA, USA) and Article Title: Coordinated DNA methyltransferase 3A and methyltransferase-like 7A activity reprograms the tumor microenvironment through discoidin domain receptor 1 signaling Article Snippet: .. Lentiviral particles were produced in HEK293T cells using Lipofectamine 2000 (#11668030; Thermo Fisher, Waltham, MA, USA). pHAGE-puro (#118692; Addgene, Watertown, MA, USA) was co-transfected with pSPAX2 (#12260; Addgene, Watertown, MA, USA) and pMD2.G (#12259; Addgene, Watertown, MA, USA) for overexpression. pSuper-retro-puro was packaged with gag/pol (#14887; Addgene, Watertown, MA, USA) and Expressing:Article Title: Induced Proprioceptor and Low‐Threshold Mechanoreceptor Neurons Derived from Human Pluripotent Stem Cells Exhibit Distinct Functional Mechanosensory Properties Article Snippet: For lentiviral production, HEK293T cells were passaged with Accutase (#00‐4555‐56, ThermoFisher) and were seeded at a density of 5000 000 cells/T75 flask. .. Lentiviral particles were produced 24 h after seeding (90–100% confluence) by co‐transfecting 12 μg of the plasmid encoding for the expression vector of interest, or 12 μg of the reverse tetracycline transactivator vector FUW‐M2rtTA (#20342, Addgene), with the lentiviral packaging plasmids: 6 μg pMDL (#12251, Addgene), 3 μg Article Title: Induced Proprioceptor and Low-Threshold Mechanoreceptor Neurons Derived from Human Pluripotent Stem Cells Exhibit Distinct Functional Mechanosensory Properties. Article Snippet: For lentiviral production, HEK293T cells were passaged with Accutase (#00-4555-56, ThermoFisher) and were seeded at a density of 5000 000 cells/T75 flask. .. Lentiviral particles were produced 24 h after seeding (90–100% confluence) by co-transfecting 12 μg of the plasmid encoding for the expression vector of interest, or 12 μg of the reverse tetracycline transactivator vector FUW-M2rtTA (#20342, Addgene), with the lentiviral packaging plasmids: 6 μg pMDL (#12251, Addgene), 3 μg |
![<t>HIV</t> infection induces miR-422a in CD4+ T cells via expression of Nef (A) Primary CD4+ T cells were stimulated with anti-CD3/CD28 beads for 3 days, then infected with HIV-1 NL4-3 . The spreading infection was measured by evaluating supernatant p24 concentrations by ELISA at each time point. (B and C) The primary (B) and mature miR-422a expression levels (C) were measured by RT-qPCR after infection with HIV-1 in primary CD4+ T cells, as described in (A). (D) Stimulated primary CD4+ T cells were infected with an HIV-1 single-round reporter virus <t>(VSVG-pseudotyped</t> HIV NL4-3 ΔEnv EGFP reporter virus) for 3 days. Cells were then sorted based on EGFP expression using the Sony MA900 multi-application cell sorter and harvested for RNA isolation. miR-422a expression was measured by RT-qPCR. (E) Stimulated primary CD4+ T cells were infected with HIV-1 single-round reporter virus (VSVG-pseudotyped HIV NL4-3 ΔEnv ΔNef GFP reporter virus) for 3 days. Cells were then sorted based on GFP expression for RNA isolation. miR-422a expression was measured by RT-qPCR. (F) Nef was nucleofected into stimulated primary CD4+ T cells for 48 h. Cells were then collected to evaluate miR-422a expression by RT-qPCR. G. Nef was nucleofected into stimulated primary CD4+ T cells for 48 h. Cells were then stained with anti-CD25 and anti-69 antibodies to detect CD25 and CD69 expression by flow cytometry. Each dot represents data from one donor. Data are representative of the results of three independent experiments ( n = 3 biologically independent samples, mean ± SEM). Statistical significance was analyzed by unpaired or paired student t tests. p ≤ 0.05 [∗], p ≤ 0.01 [∗∗], p ≤ 0.001 [∗∗∗], p ≤ 0.0001 [∗∗∗∗].](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_4541/pmc12914541/pmc12914541__gr2.jpg)