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k1087  (MedChemExpress)


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    Structured Review

    MedChemExpress k1087
    K1087, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vf+488/VF+488+Click-iT+EdU+Universal+Cell+Proliferation+Detection+Kit/pmc13156996-53-9-5
    Average 94 stars, based on 5 article reviews
    k1087 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Single-cell transcriptomics identifies PDGFRA + progenitors orchestrating angiogenesis and periodontal tissue regeneration
    Article Snippet: .. DFSCs and SCAP were seeded in 24-well plates (Corning, USA) at 1 × 10 4 cells per well and incubated for 24 h. Cells were then treated with EdU at a working concentration of 10 μmol/L in 200 μL of culture medium for 48 h. Afterward, the cells were detected using a VF 488 Click-iT EdU universal cell proliferation detection kit (HY-K1087, MCE, China) according to the manufacturer’s instructions. ..

    Article Title: Single-cell transcriptomics identifies PDGFRA + progenitors orchestrating angiogenesis and periodontal tissue regeneration.
    Article Snippet: .. EdU assay DFSCs and SCAP were seeded in 24-well plates (Corning, USA) at 1 × 104 cells per well and incubated for 24 h. Cells were then treated with EdU at a working concentration of 10 μmol/L in 200 μL of culture medium for 48 h. Afterward, the cells were detected using a VF 488 Click-iT EdU universal cell proliferation detection kit (HY-K1087, MCE, China) according to the manufacturer’s instructions. ..

    Concentration Assay:

    Article Title: Single-cell transcriptomics identifies PDGFRA + progenitors orchestrating angiogenesis and periodontal tissue regeneration
    Article Snippet: .. DFSCs and SCAP were seeded in 24-well plates (Corning, USA) at 1 × 10 4 cells per well and incubated for 24 h. Cells were then treated with EdU at a working concentration of 10 μmol/L in 200 μL of culture medium for 48 h. Afterward, the cells were detected using a VF 488 Click-iT EdU universal cell proliferation detection kit (HY-K1087, MCE, China) according to the manufacturer’s instructions. ..

    Article Title: Single-cell transcriptomics identifies PDGFRA + progenitors orchestrating angiogenesis and periodontal tissue regeneration.
    Article Snippet: .. EdU assay DFSCs and SCAP were seeded in 24-well plates (Corning, USA) at 1 × 104 cells per well and incubated for 24 h. Cells were then treated with EdU at a working concentration of 10 μmol/L in 200 μL of culture medium for 48 h. Afterward, the cells were detected using a VF 488 Click-iT EdU universal cell proliferation detection kit (HY-K1087, MCE, China) according to the manufacturer’s instructions. ..

    EdU Assay:

    Article Title: Single-cell transcriptomics identifies PDGFRA + progenitors orchestrating angiogenesis and periodontal tissue regeneration.
    Article Snippet: .. EdU assay DFSCs and SCAP were seeded in 24-well plates (Corning, USA) at 1 × 104 cells per well and incubated for 24 h. Cells were then treated with EdU at a working concentration of 10 μmol/L in 200 μL of culture medium for 48 h. Afterward, the cells were detected using a VF 488 Click-iT EdU universal cell proliferation detection kit (HY-K1087, MCE, China) according to the manufacturer’s instructions. ..



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    PDGFRA + DFSCs do not show functional superiority over PDGFRA − DFSCs in vitro. a Representative images of colonies formed by DFSCs (scale bar = 250 μm) and EdU staining images showing DFSC <t>proliferation</t> (scale bar = 50 μm). b Alizarin red S staining images showing DFSC osteogenesis. Scale bar = 100 μm. c Quantification of colony diameter in CFU assay. d Quantification of EdU + cells percent (%). e Quantification of fold change of mineralized area. f Quantification of the fold change of ALP activity. Data were presented as mean ± SD. n = 3 per group. ns not significant ( P > 0.05)
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    Image Search Results


    PDGFRA + DFSCs do not show functional superiority over PDGFRA − DFSCs in vitro. a Representative images of colonies formed by DFSCs (scale bar = 250 μm) and EdU staining images showing DFSC proliferation (scale bar = 50 μm). b Alizarin red S staining images showing DFSC osteogenesis. Scale bar = 100 μm. c Quantification of colony diameter in CFU assay. d Quantification of EdU + cells percent (%). e Quantification of fold change of mineralized area. f Quantification of the fold change of ALP activity. Data were presented as mean ± SD. n = 3 per group. ns not significant ( P > 0.05)

    Journal: International Journal of Oral Science

    Article Title: Single-cell transcriptomics identifies PDGFRA + progenitors orchestrating angiogenesis and periodontal tissue regeneration

    doi: 10.1038/s41368-025-00384-6

    Figure Lengend Snippet: PDGFRA + DFSCs do not show functional superiority over PDGFRA − DFSCs in vitro. a Representative images of colonies formed by DFSCs (scale bar = 250 μm) and EdU staining images showing DFSC proliferation (scale bar = 50 μm). b Alizarin red S staining images showing DFSC osteogenesis. Scale bar = 100 μm. c Quantification of colony diameter in CFU assay. d Quantification of EdU + cells percent (%). e Quantification of fold change of mineralized area. f Quantification of the fold change of ALP activity. Data were presented as mean ± SD. n = 3 per group. ns not significant ( P > 0.05)

    Article Snippet: DFSCs and SCAP were seeded in 24-well plates (Corning, USA) at 1 × 10 4 cells per well and incubated for 24 h. Cells were then treated with EdU at a working concentration of 10 μmol/L in 200 μL of culture medium for 48 h. Afterward, the cells were detected using a VF 488 Click-iT EdU universal cell proliferation detection kit (HY-K1087, MCE, China) according to the manufacturer’s instructions.

    Techniques: Functional Assay, In Vitro, Staining, Colony-forming Unit Assay, Activity Assay

    Endothelial PDGFBB treatment improves the functionality of PDGFRA + DFSCs. a Representative images of colonies formed by DFSCs (scale bar = 250 μm) and EdU staining images showing DFSC proliferation (scale bar = 50 μm). b Alizarin red S staining images showing DFSC osteogenesis. Scale bar = 100 μm. c Quantification of colony diameter in CFU assay. d Quantification of EdU + cells percent (%). e Quantification of fold change of mineralized area. f Quantification of the fold change of ALP activity. Data were presented as mean ± SD. n = 3 per group. * P < 0.05; ** P < 0.01; *** P < 0.001; ns not significant ( P > 0.05)

    Journal: International Journal of Oral Science

    Article Title: Single-cell transcriptomics identifies PDGFRA + progenitors orchestrating angiogenesis and periodontal tissue regeneration

    doi: 10.1038/s41368-025-00384-6

    Figure Lengend Snippet: Endothelial PDGFBB treatment improves the functionality of PDGFRA + DFSCs. a Representative images of colonies formed by DFSCs (scale bar = 250 μm) and EdU staining images showing DFSC proliferation (scale bar = 50 μm). b Alizarin red S staining images showing DFSC osteogenesis. Scale bar = 100 μm. c Quantification of colony diameter in CFU assay. d Quantification of EdU + cells percent (%). e Quantification of fold change of mineralized area. f Quantification of the fold change of ALP activity. Data were presented as mean ± SD. n = 3 per group. * P < 0.05; ** P < 0.01; *** P < 0.001; ns not significant ( P > 0.05)

    Article Snippet: DFSCs and SCAP were seeded in 24-well plates (Corning, USA) at 1 × 10 4 cells per well and incubated for 24 h. Cells were then treated with EdU at a working concentration of 10 μmol/L in 200 μL of culture medium for 48 h. Afterward, the cells were detected using a VF 488 Click-iT EdU universal cell proliferation detection kit (HY-K1087, MCE, China) according to the manufacturer’s instructions.

    Techniques: Staining, Colony-forming Unit Assay, Activity Assay