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vestashield vibrance antifade mounting medium  (Vector Laboratories)


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    Vector Laboratories vestashield vibrance antifade mounting medium
    Vestashield Vibrance Antifade Mounting Medium, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 885 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vestashield+mounting+medium/VECTASHIELD+Vibrance+Antifade+Mounting+Medium/bio_rxiv__2025__09__15__676190-152-5-10
    Average 99 stars, based on 885 article reviews
    vestashield vibrance antifade mounting medium - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Evidence for in Vivo Nicotine-Induced Alveolar Interstitial Fibroblast-to-Myofibroblast Transdifferentiation
    Article Snippet: The slides were then washed × 3 with PBS, with double distilled water × 2, and were then incubated with oil red O (Sigma, St. Louis, MO) for 15 to 30 minutes. .. Slides were rinsed × 3 for 5 minutes and then mounted and cover-slipped with Vestashield mounting medium, with DAPI (Vector Laboratories, Inc, CA) visualization under fluorescence microscope. ..

    Article Title: Selective Expression of CSPG Receptors PTPσ and LAR in Poorly Regenerating Reticulospinal Neurons of Lamprey
    Article Snippet: .. For fluorescence staining, sections were incubated with streptavidin conjugated with Alexa Fluor 680 (1:500) in 0.04% BSA/0.05% Tween-20/PBS for 2 hours, washed in PBS, and mounted with VESTASHIELD mounting medium (Vector, Burlingame, CA, H-1000); or sections were incubated with streptavidin conjugated with alkaline phosphatase (AP) in 0.04% BSA/0.05% Tween-20/PBS for 2 hours and washed with PBS followed by SMT buffer (100 mM NaCl, 50 mM MgCl 2 , 100 mM Tris, pH 9.5, 0.1% Tween-20). .. The chromogenic reaction was carried out in a solution containing 20 μl of nitro-blue tetrazolium/5-bromo-4-chloro-3-inodolyl phosphate (NBT/BCIP) stock solution (Roche Applied Science) in every 1 ml of SMT on ice in the dark for 10 minutes or until the reaction was completed (staining was monitored under a dissecting microscope).

    Article Title: Selective expression of CSPG receptors PTPσ and LAR in poorly regenerating reticulospinal neurons of lamprey.
    Article Snippet: .. For fluorescence staining, sections were incubated with streptavidin conjugated with Alexa Fluor 680 (1:500) in 0.04% BSA/0.05% Tween20/PBS for 2 hours, washed in PBS, and mounted with VESTASHIELD mounting medium (Vector, Burlingame, CA, H-1000); or sections were incubated with streptavidin conjugated with alkaline phosphatase (AP) in 0.04% BSA/0.05% Tween-20/PBS for 2 hours and washed with PBS followed by SMT buffer (100 mM NaCl, 50 mM MgCl2, 100 mM Tris, pH 9.5, 0.1% Tween-20). .. The chromogenic reaction was carried out in a solution containing 20 ll of nitro-blue tetrazolium/5-bromo-4chloro-3-inodolyl phosphate (NBT/BCIP) stock solution (Roche Applied Science) in every 1 ml of SMT on ice in the dark for 10 minutes or until the reaction was completed (staining was monitored under a dissecting microscope).

    Article Title: Treatment for nicotine-induced lung disease using peroxisome proliferator-activated receptor gamma agonists
    Article Snippet: The slides were then washed 3× with PBS and with double-distilled water 2× and were then incubated with oil red 0 (Sigma) for 15-30 min. .. Slides were rinsed 3× for 5 min and then mounted and coverslipped with Vestashield mounting medium with 4′,6′-diamidino-2-phenylindole (Vector Laboratories) visualization under a fluorescence microscope. ..

    Microscopy:

    Article Title: Evidence for in Vivo Nicotine-Induced Alveolar Interstitial Fibroblast-to-Myofibroblast Transdifferentiation
    Article Snippet: The slides were then washed × 3 with PBS, with double distilled water × 2, and were then incubated with oil red O (Sigma, St. Louis, MO) for 15 to 30 minutes. .. Slides were rinsed × 3 for 5 minutes and then mounted and cover-slipped with Vestashield mounting medium, with DAPI (Vector Laboratories, Inc, CA) visualization under fluorescence microscope. ..

    Article Title: Treatment for nicotine-induced lung disease using peroxisome proliferator-activated receptor gamma agonists
    Article Snippet: The slides were then washed 3× with PBS and with double-distilled water 2× and were then incubated with oil red 0 (Sigma) for 15-30 min. .. Slides were rinsed 3× for 5 min and then mounted and coverslipped with Vestashield mounting medium with 4′,6′-diamidino-2-phenylindole (Vector Laboratories) visualization under a fluorescence microscope. ..

    Staining:

    Article Title: Selective Expression of CSPG Receptors PTPσ and LAR in Poorly Regenerating Reticulospinal Neurons of Lamprey
    Article Snippet: .. For fluorescence staining, sections were incubated with streptavidin conjugated with Alexa Fluor 680 (1:500) in 0.04% BSA/0.05% Tween-20/PBS for 2 hours, washed in PBS, and mounted with VESTASHIELD mounting medium (Vector, Burlingame, CA, H-1000); or sections were incubated with streptavidin conjugated with alkaline phosphatase (AP) in 0.04% BSA/0.05% Tween-20/PBS for 2 hours and washed with PBS followed by SMT buffer (100 mM NaCl, 50 mM MgCl 2 , 100 mM Tris, pH 9.5, 0.1% Tween-20). .. The chromogenic reaction was carried out in a solution containing 20 μl of nitro-blue tetrazolium/5-bromo-4-chloro-3-inodolyl phosphate (NBT/BCIP) stock solution (Roche Applied Science) in every 1 ml of SMT on ice in the dark for 10 minutes or until the reaction was completed (staining was monitored under a dissecting microscope).

    Article Title: Selective expression of CSPG receptors PTPσ and LAR in poorly regenerating reticulospinal neurons of lamprey.
    Article Snippet: .. For fluorescence staining, sections were incubated with streptavidin conjugated with Alexa Fluor 680 (1:500) in 0.04% BSA/0.05% Tween20/PBS for 2 hours, washed in PBS, and mounted with VESTASHIELD mounting medium (Vector, Burlingame, CA, H-1000); or sections were incubated with streptavidin conjugated with alkaline phosphatase (AP) in 0.04% BSA/0.05% Tween-20/PBS for 2 hours and washed with PBS followed by SMT buffer (100 mM NaCl, 50 mM MgCl2, 100 mM Tris, pH 9.5, 0.1% Tween-20). .. The chromogenic reaction was carried out in a solution containing 20 ll of nitro-blue tetrazolium/5-bromo-4chloro-3-inodolyl phosphate (NBT/BCIP) stock solution (Roche Applied Science) in every 1 ml of SMT on ice in the dark for 10 minutes or until the reaction was completed (staining was monitored under a dissecting microscope).

    Incubation:

    Article Title: Selective Expression of CSPG Receptors PTPσ and LAR in Poorly Regenerating Reticulospinal Neurons of Lamprey
    Article Snippet: .. For fluorescence staining, sections were incubated with streptavidin conjugated with Alexa Fluor 680 (1:500) in 0.04% BSA/0.05% Tween-20/PBS for 2 hours, washed in PBS, and mounted with VESTASHIELD mounting medium (Vector, Burlingame, CA, H-1000); or sections were incubated with streptavidin conjugated with alkaline phosphatase (AP) in 0.04% BSA/0.05% Tween-20/PBS for 2 hours and washed with PBS followed by SMT buffer (100 mM NaCl, 50 mM MgCl 2 , 100 mM Tris, pH 9.5, 0.1% Tween-20). .. The chromogenic reaction was carried out in a solution containing 20 μl of nitro-blue tetrazolium/5-bromo-4-chloro-3-inodolyl phosphate (NBT/BCIP) stock solution (Roche Applied Science) in every 1 ml of SMT on ice in the dark for 10 minutes or until the reaction was completed (staining was monitored under a dissecting microscope).

    Article Title: Selective expression of CSPG receptors PTPσ and LAR in poorly regenerating reticulospinal neurons of lamprey.
    Article Snippet: .. For fluorescence staining, sections were incubated with streptavidin conjugated with Alexa Fluor 680 (1:500) in 0.04% BSA/0.05% Tween20/PBS for 2 hours, washed in PBS, and mounted with VESTASHIELD mounting medium (Vector, Burlingame, CA, H-1000); or sections were incubated with streptavidin conjugated with alkaline phosphatase (AP) in 0.04% BSA/0.05% Tween-20/PBS for 2 hours and washed with PBS followed by SMT buffer (100 mM NaCl, 50 mM MgCl2, 100 mM Tris, pH 9.5, 0.1% Tween-20). .. The chromogenic reaction was carried out in a solution containing 20 ll of nitro-blue tetrazolium/5-bromo-4chloro-3-inodolyl phosphate (NBT/BCIP) stock solution (Roche Applied Science) in every 1 ml of SMT on ice in the dark for 10 minutes or until the reaction was completed (staining was monitored under a dissecting microscope).

    Article Title: Generation of a human iPS cell line (CABi003-A) from a patient with age-related macular degeneration carrying the CFH Y402H polymorphism.
    Article Snippet: .. After incubation, samples were washed 3 times in 0.2% Triton-X100/PBS, and incubated with the secondary antibodies in blocking solution at room temperature for 1 h. After 3 washes, coverslips were mounted with Vestashield mounting medium (Vector H1200) containing 4,6-diamidino-2-phenylindole (DAPI). .. Imaging was performed in a fluorescence microscope Leica DM6000 B. Antibodies are listed in Table 2.

    Saline:

    Article Title: Composition for preventing or treating a spinal cord injury
    Article Snippet: The next day, it was washed with saline, and then treated with a secondary antibody conjugated with Alexa Fluor 488 (1:400, Invitrogen) or cyanine 3 (1:500, Jackson ImmunoResearch) for 1 hour at a room temperature. .. After washing twice with saline, slides were mounted with Vestashield mounting medium (DAPI, Vector Laboratories, Burlingame, Calif.) including DAPI (4,6-diamidino-2-phenylindole). .. Finally, it was observed with Leica CTR 4000 Fluorescence microscopy or Zeiss LSM 510 META confocal microscopy, and the results were shown in FIG. 6.

    Blocking Assay:

    Article Title: Generation of a human iPS cell line (CABi003-A) from a patient with age-related macular degeneration carrying the CFH Y402H polymorphism.
    Article Snippet: .. After incubation, samples were washed 3 times in 0.2% Triton-X100/PBS, and incubated with the secondary antibodies in blocking solution at room temperature for 1 h. After 3 washes, coverslips were mounted with Vestashield mounting medium (Vector H1200) containing 4,6-diamidino-2-phenylindole (DAPI). .. Imaging was performed in a fluorescence microscope Leica DM6000 B. Antibodies are listed in Table 2.



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    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Integration of transcriptomes of senescent cell models with multi-tissue patient samples reveals reduced COL6A3 as an inducer of senescence

    doi: 10.1016/j.celrep.2023.113371

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Cells were washed with dH2O and each coverslip mounted on a top of a standard microscopy glass slide using 5 μL of Vestashield Mounting Medium for fluorescence (Vector Laboratory Inc H-1000) and images captured.

    Techniques: Imaging, Recombinant, SYBR Green Assay, Control, shRNA, Plasmid Preparation, Fluorescence, Software, Expressing