Journal: Materials Today Bio

Article Title: Spatiotemporal piezoelectric microspheres for wireless endometrial repair with improved pregnancy outcomes

doi: 10.1016/j.mtbio.2026.102915

Figure Lengend Snippet: Fabrication of spatiotemporal piezoelectric microspheres and functional mechanism for endometrial repair. a, Overview of the synthesis process for spatiotemporal piezoelectric microspheres. b-c, In endometrium-injured rats, the present spatiotemporal piezoelectric microspheres facilitated tissue restoration. Leveraging ultrasound-enhanced transdermal delivery, the SPCM exerted anti-inflammatory, antibacterial, promoted vascular regeneration and cell proliferation. Collectively, these actions promoted healing of the damaged endometrium and markedly improved reproductive outcomes. BTO: BaTiO 3 ; UV: ultrasound; ACT: acteoside; PDA: polydopamine; SPCM: spatiotemporally programmable core-shell microsphere. Schemes created with BioRender.com .

Article Snippet: Acteoside (CAS:61276-17-3) was bought from Shanghai Yuanye Bio-Technology Co., Ltd. Lipopolysaccharide (LPS) was bought from Beijing Solarbio Science & Technology Co., Ltd (Beijing, China). α-SMA, MVD, VEGF, IL-6, IL-10 antibodies for rat were manufactured by Proteintech Group, Inc. (Chicago, USA).

Techniques: Functional Assay

Journal: Neurotherapeutics

Article Title: Acteoside exerts neuroprotective effects by preventing α-synuclein aggregation and oxidative stress in models of Parkinson’s disease

doi: 10.1016/j.neurot.2025.e00825

Figure Lengend Snippet: Acteoside binds to α-syn. (A) RPLC-MS chromatograms in negative ion mode of V. thapsus crude extract (upper panel) and V. thapsus crude extract fished by α-syn (lower panel). Extracted ion acteoside, 623.19 m / z . (B) Chemical structure of acteoside. (C) Acteoside-α-syn interaction by Native-MS. Enlargement in the region of α-syn 7+ charge state of Native-MS spectra, obtained at distinct acteoside concentrations (0–280 μM). The peaks corresponding to unbound α-syn (blue circle) and 1:1 α-syn-acteoside complex (red circle) are labeled. (D) Histogram reporting the fraction of unbound α-syn (blue) and α-syn-acteoside complex (red) as a function of the analyte charge state. (E) Fraction of protein in the bound state vs. total ligand concentration. The solid line represents the non-linear fit of the experimental data with a 1:1 binding curve ( K d ∼0.9 mM).

Article Snippet: Commercial α-syn (Merck Millipore) and acteoside (Merck) powders were individually resuspended in 100 mM ammonium acetate pH 7 at 1 mg/mL stock concentration.

Techniques: Labeling, Concentration Assay, Binding Assay

Journal: Neurotherapeutics

Article Title: Acteoside exerts neuroprotective effects by preventing α-synuclein aggregation and oxidative stress in models of Parkinson’s disease

doi: 10.1016/j.neurot.2025.e00825

Figure Lengend Snippet: Acteoside inhibits α-syn aggregation. (A) α-syn aggregation process, followed by ThT fluorescence after 72 h, in the absence (cnt 1, solvent control 2.5 % ethanol) or presence of V. thapsus (solvent 2.5 % ethanol) and in the absence (cnt 2, solvent control water) or presence of acteoside (100 μM or 50 μM in aqueous solution). (B) Kinetic of α-syn aggregation process, detected by ThT fluorescence, in the absence (cnt) or presence of acteoside (100 μM or 50 μM), with a zoom of the 20–30 h time interval. (C) TEM images of 70 μM α-syn after 72 h of aggregation in the absence (cnt) or presence of acteoside (50 μM in aqueous solution). (D) α-syn fibril elongation: 35 μM monomeric protein was incubated with 1 μM of preformed fibrils, detected by ThT fluorescence, in the absence (cnt) or presence of increasing concentration of acteoside (1 μM, 25 μM, 50 μM and 100 μM). Representative data from n = 3 independent experiments.

Article Snippet: Commercial α-syn (Merck Millipore) and acteoside (Merck) powders were individually resuspended in 100 mM ammonium acetate pH 7 at 1 mg/mL stock concentration.

Techniques: Fluorescence, Solvent, Control, Incubation, Concentration Assay

Journal: Neurotherapeutics

Article Title: Acteoside exerts neuroprotective effects by preventing α-synuclein aggregation and oxidative stress in models of Parkinson’s disease

doi: 10.1016/j.neurot.2025.e00825

Figure Lengend Snippet: Acetoside activates the NRF2 signaling pathway. (A) Temporal course of intracellular/cell-associated level of acteoside in neuroblastoma cells. Data are expressed as pmol acteoside/μg proteins. (B) SH-SY5Y cells were grown for 72 h in the absence or presence of 5 μM α-syn, obtained after 72 h of aggregation without or with 1 mM acteoside. ROS level was evaluated by H 2 DCFDA staining. ∗ p < 0.05. (C) SH-SY5Y pTet-SNCA-FLAG cells were treated with 6 μg/mL doxycycline to induce α-syn overexpression, in the absence or presence of 100 μM acteoside for 48 h. Cells were fixed and immunostained with anti-Syn33 antibody (specific for oligomeric species). Graphs represent mean ± SD of oligomeric α-syn fluorescence quantified as Integrated Density (IntDen) using ImageJ software. (D) SH-SY5Y pTet-SNCA-FLAG cells were treated with 6 μg/mL doxycycline to induce α-syn overexpression, in the absence or presence of 100 μM acteoside for 48 h. The level of α-syn (both endogenous and FLAG-tagged overexpressed) was evaluated by Western blot. Vinculin was used as loading control. (E) Western analysis using anti-NRF2 and anti-vinculin antibody on protein extracts from neuroblastoma cells grown for 48 h in the absence (cnt) or in the presence of acteoside (100 μM). (F) Quantitative analysis of NRF2 protein level in (E). (G) Immunofluorescence images of neuroblastoma cells treated with 100 μM of acteoside for 48 h. Fixed cells were immunostained with anti-NRF2 antibody. Nuclei were stained by DAPI (blue). (H) Quantitative analysis of nuclear localization in (G) performed using Cell-ACDC software. (I) Relative mRNA expression levels of NQO1 and HMOX1 genes in neuroblastoma cells grown in the absence (cnt) or in the presence of acteoside (100 μM) for 48 h. (J) SH-SY5Y cells were pretreated with 100 μM acteoside and/or 5 μM ML385 (NRF2 inhibitor) for 2 h, then treated with 5 μM α-syn fibrils for 72 h. ROS level was evaluated by H 2 DCFDA staining. Data represent mean ± SD from n = 3 independent experiments,∗ p < 0.05.

Article Snippet: Commercial α-syn (Merck Millipore) and acteoside (Merck) powders were individually resuspended in 100 mM ammonium acetate pH 7 at 1 mg/mL stock concentration.

Techniques: Staining, Over Expression, Fluorescence, Software, Western Blot, Control, Immunofluorescence, Expressing

Journal: Neurotherapeutics

Article Title: Acteoside exerts neuroprotective effects by preventing α-synuclein aggregation and oxidative stress in models of Parkinson’s disease

doi: 10.1016/j.neurot.2025.e00825

Figure Lengend Snippet: Effect of acteoside on DJ-1β mutant flies. (A) Motor performance of DJ-1β mutant flies treated with 250 and 400 μM acteoside was evaluated by performing a climbing assay ( n = 80–120 5-day-old female flies for each condition). (B) Protein carbonylation level in DJ-1β mutant flies treated with 250 and 400 μM acteoside was analyzed using 2,4-dinitrophenylhydrazine (DNPH) derivatization, data are expressed as arbitrary units (a.u.) per mg of protein. Results were normalized to data obtained from flies cultured in vehicle medium (0.1 % DMSO). Values are expressed as mean ± SD from three independent experiments in which three biological replicates were used. Groups were statistically compared by one-way ANOVA analysis followed by Tukey’s post hoc test (∗ p < 0.05). (C) Motor performance of DJ-1β mutant flies treated post-eclosion with 400 μM acteoside was evaluated by performing a climbing assay 5 and 10 days after treatment initiation ( n = 80–120 female flies per condition). (D) Protein carbonylation level in DJ-1β mutant flies treated post-eclosion with 400 μM acteoside was analyzed 10 days after treatment using 2,4-dinitrophenylhydrazine (DNPH) derivatization, data are expressed as arbitrary units (a.u.) per mg of protein. Results were normalized to data obtained from control flies cultured in vehicle medium (0.1 % DMSO). Values are expressed as mean ± SD from three independent experiments. ∗ p < 0.05.

Article Snippet: Commercial α-syn (Merck Millipore) and acteoside (Merck) powders were individually resuspended in 100 mM ammonium acetate pH 7 at 1 mg/mL stock concentration.

Techniques: Mutagenesis, Climbing Assay, Derivatization, Cell Culture, Control