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autofluorescence  (Vector Laboratories)


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    Structured Review

    Vector Laboratories autofluorescence
    Autofluorescence, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 904 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+trueview+autofluorescence/Vector%C2%AE+TrueVIEW%C2%AE+Autofluorescence+Quenching+Kit/pmc12919959-231-1-4
    Average 99 stars, based on 904 article reviews
    autofluorescence - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    other:

    Article Title: Characterization of cutaneous wound healing in swine
    Article Snippet: The paraffin sections were deparaffinized, retrieved for antigens with 1 mM EDTA (pH 8.0) with 0.05% Tween 20 at 95 °C for 25 minutes, quenched with Vector TrueVIEW autofluorescence quenching Kit (catalog number SP-8400-15, Vector labs, Newark, CA), and blocked with 10% donkey serum with 3% BSA for 2 hours.

    Article Title: Chronic cold exposure induces plasticity of mitochondrial calcium uptake in beige and brown fat of UCP1-deficient mice
    Article Snippet: After extensive washing in PBS, secondary antibodies anti-mouse Alexa 594 (Invitrogen) at 1:500 were added for 1 h. Slides were quenched with Vector TrueVIEW Autofluorescence Quenching Kit (SP-8400-15), and coverslips were mounted with VECTASHIELD Vibrance Antifade Mounting Medium (Vector Laboratories, H-1700-10).

    Article Title: CD44 expression in renal tubules during maladaptive repair is a potential marker for the transition from acute kidney injury to chronic kidney disease in rats.
    Article Snippet: All IF sections were treated with a Vector TrueVIEW Autofluorescence Quenching Kit (Vector Laboratories, Burlingame, CA, USA) to eliminate autofluorescence and then mounted with a Vibrance Antifade Mounting Medium containing DAPI (Vector Laboratories).

    Article Title: Electrophilic compound screening identifies GPX4-dependent ferroptosis as a senescence vulnerability.
    Article Snippet: Following PBS washes, tissue autofluorescence was quenched using the Vector TrueVIEW Autofluorescence Quenching Kit according to the manufacturer’s instructions.

    Incubation:

    Article Title: The Colonic Mucus Layer is Thinner and is Associated with Goblet Cell Hyperplasia in the db/db Mouse Model of Type 2 Diabetes
    Article Snippet: Unbound secondary antibodies were washed off with PBS (2 x 10 min), then sections were incubated with the nuclear marker DAPI (1: 1000 in PBS, 5 min at RT; Sigma Aldrich). .. Tissues were washed (1 x 10 min PBS), then incubated with Vector TrueVIEW Autofluorescence Quenching solution (Vector Laboratories) for 5 min at RT. .. Sections were then washed (1 x 10 min PBS) before mounting with ProLong Diamond Antifade mounting medium (Thermo Fisher Scientific).

    Article Title: Aurora kinase A enables collective invasion and metastasis by endowing a leader cell phenotype and stabilizing Eplin-mediated cohesion with follower cells
    Article Snippet: .. Each section was then blocked with 1% BSA 5% horse serum in PBS for 1 h at room temperature, permeabilized with 0.3% Triton-X 100 in PBS, then incubated with primary antibody at 4°C overnight, secondary antibody at room temperature for 2 hours, and used the Vector TrueVIEW Autofluorescence Quenching Kit (Vector Laboratories, SP-8400-15) per the manufacturer’s recommendation before incubating with Hoechst 33342 (1: 2,000) for 10 minutes at room temperature and mounting with VECTASHIELD Vibrance Antifade Mounting Medium. ..

    Article Title: Impaired removal of dying brain cells by microglia in Gpr34 deficient mice.
    Article Snippet: .. After all antibodies were washed off, slides were incubated with DAPI (Fisher Scientific) at 1:10,000 RT for 5 minutes before being washed an incubated with Vector TrueVIEW Autofluorescence Quenching Kit for 5 minutes at RT. .. Slides were then washed with water, and mounted with VectaShield hard set antifade mounting media (Vector) and left to dry at 4*C overnight.

    Staining:

    Article Title: Cell fate specification during respiratory development requires ARID1A-containing canonical BAF complex activity.
    Article Snippet: The corresponding secondary antibodies (1:200) used include: Goat anti-Guinea Pig IgG Alexa Fluor 555 (Invitrogen, A21435), Goat anti-Guinea Pig IgG Alexa Fluor 647 (Invitrogen, A21450), Goat anti-Mouse IgG1 Alexa AR TI CL E IN P RE SS | Lee et al. | Epithelial Arid1a patterns lung development Fluor 488 (Invitrogen, A21121), Goat anti-Mouse IgG2b Alexa Fluor 568 (Invitrogen, A21144), Goat anti-Mouse IgG Alexa Fluor 568 (Invitrogen, A11001), Goat anti-Rabbit IgG Alexa Fluor 568 (Invitrogen, A11036), Donkey anti-Rabbit IgG Alexa Fluor 647 (Invitrogen, A31573), Goat antiRat Alexa Fluor 647 (Invitrogen, A21247), and Donkey anti-Goat IgG Alexa Fluor 568 (Invitrogen, A11057). .. Following secondary antibody application, the sections were quenched to remove autofluorescence using Vector TrueVIEW Autofluorescence Quenching kit (Vector Labs, SP8400), stained with DAPI (Invitrogen, D1306; 1:2000) and mounted using Prolong Gold antifade mounting medium (Invitrogen, P36930). .. Immunofluorescence for Yap (Rabbit, Cell Signaling Technology, 4912 - 1:100), Taz (Rabbit, Cell Signaling Technology, 83669 - 1:200), p-Smad1/5/9 (Rabbit, Cell Signaling Technology, 13820, 1:100) and non-phospho (Active) beta-catenin (Rabbit, Cell Signaling Technology, 8814 - 1:500) was performed using ImmPRESS® HRP Horse Anti-Rabbit IgG Polymer Detection Kit (Vector Labs, MP-7401-50) which was followed Tyramide signal amplification (TSA Plus Cyanine 3.5, Akoya Biosciences, NEL763001KT) with an 8 min exposure time.



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    Citrullinated and Homocitrullinated Proteins/Peptides are Present in DBA/1J Metatarsophalangeal Joints. A) Representative hematoxylin + eosin (H&E) at 80× magnification and immunofluorescent micrographs at 20× magnification of joints from DBA/1J mice immunized with bovine type II collagen (arthritic: N = 9; non-arthritic: N = 10) or PBS (N = 9). Scale bars represent 500 μm. Max projections of Z-stacks show nuclei <t>(DAPI;</t> blue), citrullinated proteins/peptides (CitP; green), and homocitrullinated proteins/peptides (HomoCitP; red). Corrected total fluorescence intensity for B) CitP and C) HomoCitP in three joint structures: bone marrow, synovium, and cartilage. Graphs show the median [IQR], with each symbol representing an individual mouse. Statistical analysis was performed using Kruskal-Wallis with Dunn's multiple comparisons test, and the resulting p-values were p = 0.0046, p = 0.0084, p = 0.4076, p = 0.0143, p = 0.0136, and p = 0.1733 respectively, with p-values for pairwise comparisons indicated on the graphs. ns: not significant.
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    Citrullinated and Homocitrullinated Proteins/Peptides are Present in DBA/1J Metatarsophalangeal Joints. A) Representative hematoxylin + eosin (H&E) at 80× magnification and immunofluorescent micrographs at 20× magnification of joints from DBA/1J mice immunized with bovine type II collagen (arthritic: N = 9; non-arthritic: N = 10) or PBS (N = 9). Scale bars represent 500 μm. Max projections of Z-stacks show nuclei <t>(DAPI;</t> blue), citrullinated proteins/peptides (CitP; green), and homocitrullinated proteins/peptides (HomoCitP; red). Corrected total fluorescence intensity for B) CitP and C) HomoCitP in three joint structures: bone marrow, synovium, and cartilage. Graphs show the median [IQR], with each symbol representing an individual mouse. Statistical analysis was performed using Kruskal-Wallis with Dunn's multiple comparisons test, and the resulting p-values were p = 0.0046, p = 0.0084, p = 0.4076, p = 0.0143, p = 0.0136, and p = 0.1733 respectively, with p-values for pairwise comparisons indicated on the graphs. ns: not significant.
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    Citrullinated and Homocitrullinated Proteins/Peptides are Present in DBA/1J Metatarsophalangeal Joints. A) Representative hematoxylin + eosin (H&E) at 80× magnification and immunofluorescent micrographs at 20× magnification of joints from DBA/1J mice immunized with bovine type II collagen (arthritic: N = 9; non-arthritic: N = 10) or PBS (N = 9). Scale bars represent 500 μm. Max projections of Z-stacks show nuclei <t>(DAPI;</t> blue), citrullinated proteins/peptides (CitP; green), and homocitrullinated proteins/peptides (HomoCitP; red). Corrected total fluorescence intensity for B) CitP and C) HomoCitP in three joint structures: bone marrow, synovium, and cartilage. Graphs show the median [IQR], with each symbol representing an individual mouse. Statistical analysis was performed using Kruskal-Wallis with Dunn's multiple comparisons test, and the resulting p-values were p = 0.0046, p = 0.0084, p = 0.4076, p = 0.0143, p = 0.0136, and p = 0.1733 respectively, with p-values for pairwise comparisons indicated on the graphs. ns: not significant.
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    Image Search Results


    Citrullinated and Homocitrullinated Proteins/Peptides are Present in DBA/1J Metatarsophalangeal Joints. A) Representative hematoxylin + eosin (H&E) at 80× magnification and immunofluorescent micrographs at 20× magnification of joints from DBA/1J mice immunized with bovine type II collagen (arthritic: N = 9; non-arthritic: N = 10) or PBS (N = 9). Scale bars represent 500 μm. Max projections of Z-stacks show nuclei (DAPI; blue), citrullinated proteins/peptides (CitP; green), and homocitrullinated proteins/peptides (HomoCitP; red). Corrected total fluorescence intensity for B) CitP and C) HomoCitP in three joint structures: bone marrow, synovium, and cartilage. Graphs show the median [IQR], with each symbol representing an individual mouse. Statistical analysis was performed using Kruskal-Wallis with Dunn's multiple comparisons test, and the resulting p-values were p = 0.0046, p = 0.0084, p = 0.4076, p = 0.0143, p = 0.0136, and p = 0.1733 respectively, with p-values for pairwise comparisons indicated on the graphs. ns: not significant.

    Journal: Journal of Translational Autoimmunity

    Article Title: T cell proliferative response to a homocitrullinated peptide correlates with joint pathology in collagen induced arthritis

    doi: 10.1016/j.jtauto.2025.100345

    Figure Lengend Snippet: Citrullinated and Homocitrullinated Proteins/Peptides are Present in DBA/1J Metatarsophalangeal Joints. A) Representative hematoxylin + eosin (H&E) at 80× magnification and immunofluorescent micrographs at 20× magnification of joints from DBA/1J mice immunized with bovine type II collagen (arthritic: N = 9; non-arthritic: N = 10) or PBS (N = 9). Scale bars represent 500 μm. Max projections of Z-stacks show nuclei (DAPI; blue), citrullinated proteins/peptides (CitP; green), and homocitrullinated proteins/peptides (HomoCitP; red). Corrected total fluorescence intensity for B) CitP and C) HomoCitP in three joint structures: bone marrow, synovium, and cartilage. Graphs show the median [IQR], with each symbol representing an individual mouse. Statistical analysis was performed using Kruskal-Wallis with Dunn's multiple comparisons test, and the resulting p-values were p = 0.0046, p = 0.0084, p = 0.4076, p = 0.0143, p = 0.0136, and p = 0.1733 respectively, with p-values for pairwise comparisons indicated on the graphs. ns: not significant.

    Article Snippet: Following application of TrueView autofluorescence quencher, slides were mounted using Vectashield Vibrance Antifade mounting media with DAPI (SP-8500-15; Vector Laboratories; USA) and air dried for 2 h before imaging on the Nikon Ti2-E microscope.

    Techniques: Fluorescence