autofluorescence (Vector Laboratories)
Structured Review
Autofluorescence, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 904 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vector+trueview+autofluorescence/Vector%C2%AE+TrueVIEW%C2%AE+Autofluorescence+Quenching+Kit/pmc12919959-231-1-4
Average 99 stars, based on 904 article reviews
Images
Related Articles
other:Article Title: Characterization of cutaneous wound healing in swine Article Snippet: The paraffin sections were deparaffinized, retrieved for antigens with 1 mM EDTA (pH 8.0) with 0.05% Tween 20 at 95 °C for 25 minutes, quenched with Vector TrueVIEW autofluorescence quenching Kit (catalog number Article Title: Chronic cold exposure induces plasticity of mitochondrial calcium uptake in beige and brown fat of UCP1-deficient mice Article Snippet: After extensive washing in PBS, secondary antibodies anti-mouse Alexa 594 (Invitrogen) at 1:500 were added for 1 h. Article Title: CD44 expression in renal tubules during maladaptive repair is a potential marker for the transition from acute kidney injury to chronic kidney disease in rats. Article Snippet: All IF sections were treated with a Article Title: Electrophilic compound screening identifies GPX4-dependent ferroptosis as a senescence vulnerability. Article Snippet: Following PBS washes, tissue autofluorescence was quenched using the Incubation:Article Title: The Colonic Mucus Layer is Thinner and is Associated with Goblet Cell Hyperplasia in the db/db Mouse Model of Type 2 Diabetes Article Snippet: Unbound secondary antibodies were washed off with PBS (2 x 10 min), then sections were incubated with the nuclear marker DAPI (1: 1000 in PBS, 5 min at RT; Sigma Aldrich). .. Tissues were washed (1 x 10 min PBS), then incubated with Vector TrueVIEW Autofluorescence Quenching Article Title: Aurora kinase A enables collective invasion and metastasis by endowing a leader cell phenotype and stabilizing Eplin-mediated cohesion with follower cells Article Snippet: .. Each section was then blocked with 1% BSA 5% horse serum in PBS for 1 h at room temperature, permeabilized with 0.3% Triton-X 100 in PBS, then incubated with primary antibody at 4°C overnight, secondary antibody at room temperature for 2 hours, and used the Article Title: Impaired removal of dying brain cells by microglia in Gpr34 deficient mice. Article Snippet: .. After all antibodies were washed off, slides were incubated with DAPI (Fisher Scientific) at 1:10,000 RT for 5 minutes before being washed an incubated with Staining:Article Title: Cell fate specification during respiratory development requires ARID1A-containing canonical BAF complex activity. Article Snippet: The corresponding secondary antibodies (1:200) used include: Goat anti-Guinea Pig IgG Alexa Fluor 555 (Invitrogen, A21435), Goat anti-Guinea Pig IgG Alexa Fluor 647 (Invitrogen, A21450), Goat anti-Mouse IgG1 Alexa AR TI CL E IN P RE SS | Lee et al. | Epithelial Arid1a patterns lung development Fluor 488 (Invitrogen, A21121), Goat anti-Mouse IgG2b Alexa Fluor 568 (Invitrogen, A21144), Goat anti-Mouse IgG Alexa Fluor 568 (Invitrogen, A11001), Goat anti-Rabbit IgG Alexa Fluor 568 (Invitrogen, A11036), Donkey anti-Rabbit IgG Alexa Fluor 647 (Invitrogen, A31573), Goat antiRat Alexa Fluor 647 (Invitrogen, A21247), and Donkey anti-Goat IgG Alexa Fluor 568 (Invitrogen, A11057). .. Following secondary antibody application, the sections were quenched to remove autofluorescence using Vector TrueVIEW Autofluorescence Quenching kit ( |
![Citrullinated and Homocitrullinated Proteins/Peptides are Present in DBA/1J Metatarsophalangeal Joints. A) Representative hematoxylin + eosin (H&E) at 80× magnification and immunofluorescent micrographs at 20× magnification of joints from DBA/1J mice immunized with bovine type II collagen (arthritic: N = 9; non-arthritic: N = 10) or PBS (N = 9). Scale bars represent 500 μm. Max projections of Z-stacks show nuclei <t>(DAPI;</t> blue), citrullinated proteins/peptides (CitP; green), and homocitrullinated proteins/peptides (HomoCitP; red). Corrected total fluorescence intensity for B) CitP and C) HomoCitP in three joint structures: bone marrow, synovium, and cartilage. Graphs show the median [IQR], with each symbol representing an individual mouse. Statistical analysis was performed using Kruskal-Wallis with Dunn's multiple comparisons test, and the resulting p-values were p = 0.0046, p = 0.0084, p = 0.4076, p = 0.0143, p = 0.0136, and p = 0.1733 respectively, with p-values for pairwise comparisons indicated on the graphs. ns: not significant.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_0342/pmc12810342/pmc12810342__gr3.jpg)